BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,215	(G)_8→7	noncoding (72/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,208	0	G	.	97.2%	436.0 / 7.9	108	noncoding (79/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (2/1); new base . (51/52); total (55/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT  >  NC_000913/4606110‑4606302

tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgg                                                                                               >  1:2273790/1‑100 (MQ=255)

   gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGTCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAggggg                                                                                            <  2:2233413/100‑1 (MQ=255)

   gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAggggg                                                                                            <  1:1209200/100‑1 (MQ=255)

   gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAggggg                                                                                            <  1:1887001/100‑1 (MQ=255)

    tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggg                                                                                           <  1:985826/100‑1 (MQ=255)

    tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggg                                                                                           <  2:2328273/100‑1 (MQ=255)

    tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggg                                                                                           <  1:2428960/100‑1 (MQ=255)

     aaGGACACTAACACCTGAAGCTCGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAggggggg                                                                                          <  2:350694/100‑1 (MQ=255)

        gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggggact                                                                                       <  2:2431030/100‑4 (MQ=255)

        gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggggact                                                                                       <  1:2084304/100‑4 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  2:140822/100‑1 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  2:2883264/100‑1 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  2:2562402/100‑1 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    >  2:1485053/1‑100 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  1:1865489/100‑1 (MQ=255)

          cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    >  1:1204815/1‑100 (MQ=255)

          cacTAACACATGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACCTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  2:263484/100‑1 (MQ=25)

           acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGa                                                                                   <  1:308397/100‑1 (MQ=255)

           acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGa                                                                                   <  1:308342/100‑1 (MQ=255)

           acTAACACATGAAGCTAGCGCGTCTACCAATTCCGCCACCGTCGCACAGTCATCTTACTTTTTTTGAGAACGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTg                                                                                    <  2:2879883/99‑1 (MQ=25)

            ntAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGaa                                                                                  >  1:79958/2‑100 (MQ=255)

            cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAgggggg                                                                                           >  2:769212/1‑92 (MQ=255)

            cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGaa                                                                                  <  1:2636396/100‑1 (MQ=255)

            cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGaa                                                                                  >  1:76509/1‑100 (MQ=255)

            cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGaa                                                                                  >  1:651698/1‑100 (MQ=255)

             tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCATCGCACAAGCGTCGTACTTTTATTGATATCGCCATCG‑TTGATACGAgggggggccttgaa                                                                                   >  2:418695/1‑92 (MQ=12)

              aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAcc                                                                                <  1:2779803/100‑1 (MQ=255)

                cacCTCAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTGCGTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccc                                                                               <  2:1298455/99‑1 (MQ=37)

                 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                             <  2:2129323/100‑1 (MQ=255)

                 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                             <  1:1562102/100‑1 (MQ=255)

                 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccccc                                                                             <  1:2081296/100‑1 (MQ=255)

                 acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccc                                                                               <  2:588488/98‑1 (MQ=255)

                   cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCAc                                                                           <  1:1485060/100‑1 (MQ=255)

                     gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGt                                                                         >  1:1907098/1‑100 (MQ=255)

                      aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGCCTTGAACCCCCACGTc                                                                        >  1:883400/1‑100 (MQ=255)

                       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAAccctcccttc                                                                        >  2:1218246/1‑92 (MQ=38)

                       aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTcc                                                                       >  1:2693228/1‑100 (MQ=255)

                         cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC‑TTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTa                                                                    <  1:2822354/100‑1 (MQ=255)

                          tAGCGCGTCTACCGATTCCGCCACCTTCGCACAGTCAGCTTACTGTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTa                                                                    <  2:1157463/100‑1 (MQ=38)

                          tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTa                                                                    >  2:1779453/1‑100 (MQ=255)

                           aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCTCC‑TCGGTGGGTCCGA‑TGCGGGTACTTTAACCCCCATGTCCGTaa                                                                   >  2:2412671/1‑100 (MQ=25)

                             cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                 >  1:2595030/1‑100 (MQ=255)

                             cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGa                                                                 >  1:869772/1‑100 (MQ=255)

                              gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGaa                                                                >  2:114351/1‑100 (MQ=255)

                               cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAac                                                               <  1:611895/100‑1 (MQ=255)

                                  cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                            >  1:2419120/1‑100 (MQ=255)

                                  cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                            >  1:1691818/1‑100 (MQ=255)

                                   tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                           <  1:482236/100‑1 (MQ=255)

                                    aCCAATTCCGCCACCTTCGCACAGTCATCTTTCTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                           <  2:883399/99‑1 (MQ=255)

                                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAGGGGGGGG‑CTTTAACCGCCACGTCCGTAAGAACACtca                                                          >  2:1749480/1‑98 (MQ=38)

                                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTaa                                                          >  2:2045159/1‑100 (MQ=255)

                                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  1:2538264/1‑100 (MQ=255)

                                    aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  1:2540887/1‑100 (MQ=255)

                                     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                          >  1:2143138/1‑100 (MQ=255)

                                     ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACACCCACGTCCGTAAGAACACTAac                                                         >  2:1076015/1‑100 (MQ=255)

                                        aTTCCGCCACCTTCGCATAGTCATCTTGCTGTTTTTGCTATCGGC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                      <  2:2591860/100‑1 (MQ=25)

                                          tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAATACTAACACCTg                                                    >  1:1765973/1‑100 (MQ=255)

                                           ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCG‑CGGGGGGGACTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGa                                                   >  1:964210/1‑100 (MQ=37)

                                            cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                  >  1:405869/1‑100 (MQ=255)

                                            cGCCACCTTCGCACAGTCATCTTAC‑TTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                 >  1:2396219/1‑100 (MQ=255)

                                             gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAg                                                 >  1:1680757/1‑100 (MQ=255)

                                              ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                >  1:576756/1‑100 (MQ=255)

                                                   ttCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAgcg                                           <  2:1680744/100‑1 (MQ=255)

                                                     cGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACATGAAGCTAGcgcg                                         <  1:1942525/100‑1 (MQ=255)

                                                       cacaGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                       >  1:1649863/1‑100 (MQ=255)

                                                       cacaGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                       <  2:2693208/100‑1 (MQ=255)

                                                       cacaGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                       >  2:2418275/1‑100 (MQ=255)

                                                         caGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                     >  1:2530349/1‑100 (MQ=255)

                                                         caGTCATCTTACTTTTTTTGACATCGCC‑TCGTTTGGTGCG‑CGGCGGGGCCCTCAACCCCCACGTCCGTAAGCCCCCTAACACCCCAAGCTAGCGCGCCtc                                     >  2:2258252/1‑99 (MQ=16)

                                                           gtaaTCTAACTTTATTTGATACCGCC‑TCGTTTGGTCCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                   <  1:1529440/97‑1 (MQ=17)

                                                           gTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                   <  2:1713378/100‑1 (MQ=255)

                                                           gTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                   >  2:833776/1‑100 (MQ=255)

                                                                cTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                              >  2:658503/1‑100 (MQ=255)

                                                                cTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                              >  2:2320782/1‑100 (MQ=255)

                                                                 ttACTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                             >  2:1096246/1‑100 (MQ=255)

                                                                   tacttttttGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                           <  2:2396203/97‑1 (MQ=255)

                                                                    cTTTTTTTGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAACTCCGcc                          >  2:2341547/1‑100 (MQ=255)

                                                                     tttttttGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                            <  1:2312434/97‑1 (MQ=255)

                                                                     tttttttGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                         <  1:418696/100‑1 (MQ=255)

                                                                          ttGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                    >  2:1854424/1‑100 (MQ=255)

                                                                          ttGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                    >  1:1735717/1‑100 (MQ=255)

                                                                           tGATATCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                   >  2:2468655/1‑100 (MQ=255)

                                                                             atatCGCC‑TCGTTTGGTGCGA‑GGGGGCGACTTGCTCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                   <  2:2274475/98‑1 (MQ=14)

                                                                              tatCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                <  1:2015234/100‑1 (MQ=255)

                                                                              tatCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                <  1:2703156/100‑1 (MQ=255)

                                                                              taccGCC‑TCGTTTGGTGCGA‑GGGCTGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                   <  2:192264/94‑1 (MQ=14)

                                                                                tCGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg              <  1:114351/100‑1 (MQ=255)

                                                                                 cGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt             <  1:1749493/100‑1 (MQ=255)

                                                                                 cGCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt             >  1:2558651/1‑100 (MQ=255)

                                                                                  gCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAGCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            <  1:441399/100‑1 (MQ=255)

                                                                                  gCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            >  2:72431/1‑100 (MQ=255)

                                                                                  gCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            >  1:1051346/1‑100 (MQ=255)

                                                                                  gCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            <  1:2002855/100‑1 (MQ=255)

                                                                                  gCC‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg            >  1:2254621/1‑100 (MQ=255)

                                                                                    c‑TCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg          <  1:769214/100‑1 (MQ=255)

                                                                                       tgatgggggCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACGCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa         <  1:1409267/92‑1 (MQ=14)

                                                                                       cGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCTAGTGCGAg        >  2:2141734/1‑100 (MQ=255)

                                                                                         tttGGTGGGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa         <  1:1409390/97‑1 (MQ=255)

                                                                                             gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAatat  >  1:1728499/1‑100 (MQ=255)

                                                                                              tGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat  <  2:632529/99‑1 (MQ=255)

TCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCC‑TCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAT  >  NC_000913/4606110‑4606302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`