Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,233	(G)5→4	coding (346/1146 nt)	glxK →	glycerate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,229	0	G	.	97.7%	381.3 / 1.3	88	coding (342/1146 nt)	glxK	glycerate kinase II
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (43/43);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGC  >  NC_000913/542130‑542324                                                                                                    |                                                                                                cGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACg                                                                                                 >  2:2858821/1‑100 (MQ=255) cGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACg                                                                                                 >  2:2284820/1‑100 (MQ=255)  gACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACgg                                                                                                <  1:68107/100‑1 (MQ=255)   aCGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggg                                                                                               <  1:2745785/100‑1 (MQ=255)   aCGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggg                                                                                               <  1:196821/100‑1 (MQ=255)    cGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACgggg                                                                                              <  2:1650347/100‑1 (MQ=255)    cGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACgggg                                                                                              <  1:1694989/100‑1 (MQ=255)      ggAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggggag                                                                                            >  2:1886858/1‑98 (MQ=255)       gAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggggagt                                                                                           >  2:1919495/1‑97 (MQ=255)       gAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggggagt                                                                                           >  2:358842/1‑97 (MQ=255)        aaaaCGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACggggagtt                                                                                          <  1:2059974/100‑5 (MQ=255)         aaaCGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTa                                                                                        <  1:584105/100‑1 (MQ=255)            cggcggTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAAtt                                                                                     >  2:48396/1‑100 (MQ=255)                 gTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCa                                                                                <  1:1852289/100‑1 (MQ=255)                 gTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCa                                                                                <  2:2692656/100‑1 (MQ=255)                 gTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCa                                                                                >  2:371343/1‑100 (MQ=255)                  tGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTGATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCAt                                                                               <  2:2593410/100‑1 (MQ=255)                     ttGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATgcg                                                                            >  1:2037054/1‑100 (MQ=255)                     ttGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATgcg                                                                            >  2:2528706/1‑100 (MQ=255)                       gagaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCt                                                                          >  2:826187/1‑100 (MQ=255)                        agaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTg                                                                         >  1:199959/1‑100 (MQ=255)                        agaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTg                                                                         <  2:2233450/100‑1 (MQ=255)                         gaTGGCGGCAGCAAGTGGCTTTTTGCTGGTCGCGCCTGAAAAGCGTAATGCGTTGCTGGCCTCCAGTTTTGGGAC‑GGGGAGTTAATTCGTCATGCGCTgg                                                                        <  1:879297/100‑1 (MQ=255)                         gaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTgg                                                                        <  2:1334773/100‑1 (MQ=255)                         gaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTgg                                                                        >  1:603282/1‑100 (MQ=255)                         gaTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTgg                                                                        <  2:1882917/100‑1 (MQ=255)                          aTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGa                                                                       <  1:1978478/100‑1 (MQ=255)                          aTGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGa                                                                       <  2:1857488/100‑1 (MQ=255)                           tGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGAt                                                                      >  2:2151185/1‑100 (MQ=255)                            ggcggcAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATa                                                                     >  1:1584439/1‑100 (MQ=255)                              cggcAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATaa                                                                    >  2:2207670/1‑100 (MQ=255)                              cggcAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAAc                                                                   <  2:2859550/100‑1 (MQ=255)                              cggcAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAAc                                                                   <  1:611183/100‑1 (MQ=255)                                gcagcaAGCGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGa                                                                 >  2:661746/1‑100 (MQ=255)                                   gcaAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACAt                                                              >  2:1291445/1‑100 (MQ=255)                                   gcaAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACAt                                                              >  2:110269/1‑100 (MQ=255)                                    caAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACAtt                                                             >  1:455321/1‑100 (MQ=255)                                     aaGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTc                                                            >  2:83726/1‑100 (MQ=255)                                       gTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGc                                                          >  1:602928/1‑100 (MQ=255)                                       gTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGc                                                          >  2:300218/1‑100 (MQ=255)                                       gTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGc                                                          <  2:2173/100‑1 (MQ=255)                                        tGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGcc                                                         <  1:934580/100‑1 (MQ=255)                                        tGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGcc                                                         <  2:751807/100‑1 (MQ=255)                                        tGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTCTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGcc                                                         <  2:1602545/100‑1 (MQ=255)                                         ggCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCa                                                        <  1:2786550/100‑1 (MQ=255)                                         ggCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCa                                                        <  2:188356/100‑1 (MQ=255)                                          gCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCat                                                       >  2:405996/1‑100 (MQ=255)                                          gCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCat                                                       >  2:661982/1‑100 (MQ=255)                                          gCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCat                                                       <  1:1634882/100‑1 (MQ=255)                                          gCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCat                                                       >  1:1165846/1‑100 (MQ=255)                                           ccTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAAAAACATTCGCCata                                                      >  2:2160495/1‑100 (MQ=255)                                            cTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCatat                                                     >  2:2363740/1‑100 (MQ=255)                                                tGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATattatt                                                 <  1:2447773/100‑1 (MQ=255)                                                 gCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATtc                                                >  2:2663159/1‑100 (MQ=255)                                                 gCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATtc                                                >  2:2847367/1‑100 (MQ=255)                                                   tGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATtctc                                              <  1:1053016/100‑1 (MQ=255)                                                     gTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCg                                             <  1:2765992/100‑1 (MQ=255)                                                       cgcgCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCa                                          >  1:1866772/1‑100 (MQ=255)                                                         cgcCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCAtt                                        >  2:550960/1‑100 (MQ=255)                                                          gcCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTg                                       <  2:1065956/100‑1 (MQ=255)                                                           ccTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTgg                                      >  2:2349570/1‑100 (MQ=255)                                                           ccTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTgg                                      >  1:522901/1‑100 (MQ=255)                                                           ccTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTgg                                      >  1:1386909/1‑100 (MQ=255)                                                            cTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggc                                     >  2:2095904/1‑100 (MQ=255)                                                             tGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTTGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggc                                     <  2:103569/99‑1 (MQ=255)                                                              gAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcgg                                   >  2:130761/1‑100 (MQ=255)                                                              gAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcgg                                   >  2:130128/1‑100 (MQ=255)                                                              gAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcgg                                   >  1:380941/1‑100 (MQ=255)                                                               aaaaGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGTGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcggc                                  <  1:901574/100‑1 (MQ=255)                                                               aaaaGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcggc                                  <  1:2232902/100‑1 (MQ=255)                                                               aaaaGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcggc                                  <  1:901618/100‑1 (MQ=255)                                                               aaaaGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTggcggc                                  <  2:2307759/100‑1 (MQ=255)                                                                   gCGTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTg                              >  1:2539144/1‑100 (MQ=255)                                                                     gTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCg                            <  1:2211381/100‑1 (MQ=255)                                                                     gTAATCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCg                            >  1:590707/1‑100 (MQ=255)                                                                        aTCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGAc                          <  1:1913420/99‑1 (MQ=255)                                                                        aTCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACg                         >  1:2837390/1‑100 (MQ=255)                                                                         tCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACgg                        <  1:1093272/100‑1 (MQ=255)                                                                         tCCGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACgg                        <  1:342443/100‑1 (MQ=255)                                                                          ccGTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGt                       >  1:394541/1‑100 (MQ=255)                                                                            gTTGCTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCg                     <  2:324820/100‑1 (MQ=255)                                                                                cTGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcgg                 >  1:2532778/1‑100 (MQ=255)                                                                                 tGGCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggc                <  2:254146/100‑1 (MQ=255)                                                                                  ggCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcg               >  1:1245499/1‑100 (MQ=255)                                                                                  ggCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcg               >  2:340146/1‑100 (MQ=255)                                                                                  ggCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcg               >  2:342492/1‑100 (MQ=255)                                                                                  ggCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATCCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcg               >  2:1530120/1‑100 (MQ=255)                                                                                   gCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcgg              <  1:2667474/100‑1 (MQ=255)                                                                                   gCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcgg              <  1:987454/100‑1 (MQ=255)                                                                                   gCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcgg              <  1:1932155/100‑1 (MQ=255)                                                                                   gCCTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcggcgg              <  1:1886871/100‑1 (MQ=255)                                                                                     cTCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTa            >  1:165182/1‑100 (MQ=255)                                                                                      tCCAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTAt           <  1:83726/100‑1 (MQ=255)                                                                                        cAGTTTTGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATgg         <  1:1403795/100‑1 (MQ=255)                                                                                           ttttGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCa      <  1:300219/100‑1 (MQ=255)                                                                                            tttGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCa      <  1:2117650/99‑1 (MQ=255)                                                                                              tGGTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATgg   <  2:2037040/100‑1 (MQ=255)                                                                                               ggTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGgc  <  2:199958/100‑1 (MQ=255)                                                                                               ggTAC‑GGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGCGCGACGGTCGACGGCGGTATGGGCATGgc  >  2:2744959/1‑100 (MQ=255)                                                                                                    |                                                                                                CGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGC  >  NC_000913/542130‑542324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑