Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,757,705 (T)8→7 intergenic (+48/+16) lpp → / ← ldtE murein lipoprotein/murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,6980T.96.7% 508.6 / 8.9 122intergenic (+41/+23)lpp/ldtEmurein lipoprotein/murein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base . (69/49);  total (73/49)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATT  >  NC_000913/1757600‑1757792
                                                                                                  |                                                                                              
tGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtt                                                                                               >  2:651006/1‑100 (MQ=255)
tGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtt                                                                                               <  1:2677948/100‑1 (MQ=255)
  aCGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttt                                                                                             <  1:1298093/100‑1 (MQ=255)
   cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAttttt                                                                                            >  2:2143234/1‑100 (MQ=255)
   cGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAttttt                                                                                            >  2:2821226/1‑100 (MQ=255)
    gCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAttttt                                                                                            <  2:2587550/99‑1 (MQ=255)
    gCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACAATGTGCGCCAtttttc                                                                                           <  1:1778661/100‑2 (MQ=255)
      aGCTGGTGCTAACCAGCGTTTGGACAAGATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttg                                                                                         <  2:2307040/100‑2 (MQ=255)
      aGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttg                                                                                         <  2:221420/100‑2 (MQ=255)
       gCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttgt                                                                                        >  1:2140355/1‑98 (MQ=255)
        cTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttgtc                                                                                       <  2:2040744/100‑4 (MQ=255)
        cTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttgtc                                                                                       >  1:712473/1‑97 (MQ=255)
         tCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCAtttttttgtct                                                                                      >  1:1035606/1‑96 (MQ=255)
             gCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGcc                                                                                  >  1:2212129/1‑100 (MQ=255)
             gCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCg                                                                                 <  1:2066326/100‑1 (MQ=255)
              cTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGt                                                                                <  2:1801001/100‑1 (MQ=255)
               tAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGtt                                                                               <  1:1442226/100‑1 (MQ=255)
               tAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGtt                                                                               >  2:1329681/1‑100 (MQ=255)
                aaCCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGttt                                                                              <  2:270464/100‑1 (MQ=255)
                  ccAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTAc                                                                            >  2:2425596/1‑100 (MQ=255)
                  ccAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTAc                                                                            <  2:532652/100‑1 (MQ=255)
                    aGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCg                                                                          >  1:1495115/1‑100 (MQ=255)
                    aGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCg                                                                          <  2:1609816/100‑1 (MQ=255)
                     gCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGc                                                                         >  1:345601/1‑100 (MQ=255)
                       gTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTa                                                                       <  1:1012481/100‑1 (MQ=255)
                         cTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACt                                                                     >  1:965113/1‑100 (MQ=255)
                          tGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTg                                                                    >  2:1857291/1‑100 (MQ=255)
                           ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGc                                                                   >  1:602793/1‑100 (MQ=255)
                           ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGc                                                                   <  2:305381/100‑1 (MQ=255)
                             acaacaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGt                                                                 <  1:2482476/100‑1 (MQ=255)
                              cttccgatctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTc                                                                <  1:1456984/92‑1 (MQ=255)
                               aacaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCa                                                               <  1:371318/100‑1 (MQ=255)
                                acaTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCAc                                                              >  2:922573/1‑100 (MQ=255)
                                 caTGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGAACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCAcg                                                             >  1:1179085/1‑100 (MQ=255)
                                   tGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCAcgcg                                                           >  1:2413857/1‑100 (MQ=255)
                                     gCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTa                                                         >  2:2313927/1‑100 (MQ=255)
                                       tactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAAc                                                       <  1:885287/100‑1 (MQ=255)
                                        actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACa                                                      <  2:2140342/100‑1 (MQ=255)
                                         ctaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACat                                                     >  1:598199/1‑100 (MQ=255)
                                         ctaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACat                                                     >  2:2279786/1‑100 (MQ=255)
                                         ctaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACat                                                     <  1:437159/100‑1 (MQ=255)
                                             aTACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTc                                                 <  2:2484921/100‑1 (MQ=255)
                                             aTACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTc                                                 >  1:2870598/1‑100 (MQ=255)
                                              tACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTcc                                                <  2:2106837/100‑1 (MQ=255)
                                              tACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTcc                                                >  1:2283741/1‑100 (MQ=255)
                                               aCCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTccc                                               >  1:1231817/1‑100 (MQ=255)
                                                 cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCTGTTTACCGCTACTGCGTCACGCGTAACATATTCCCtt                                             >  2:1078732/1‑100 (MQ=255)
                                                 cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCtt                                             <  1:2240385/100‑1 (MQ=255)
                                                 cGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCtt                                             <  2:1423796/100‑1 (MQ=255)
                                                  gCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTg                                            <  2:193890/100‑1 (MQ=255)
                                                  gCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTg                                            <  1:208696/100‑1 (MQ=255)
                                                  gCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTg                                            >  1:2829348/1‑100 (MQ=255)
                                                  gCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTg                                            <  1:2320703/100‑1 (MQ=255)
                                                    aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGct                                          <  1:2425612/100‑1 (MQ=255)
                                                      gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGctct                                        >  2:2448067/1‑100 (MQ=255)
                                                      gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGctct                                        <  1:257062/100‑1 (MQ=255)
                                                      gTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGctct                                        <  1:2762799/100‑1 (MQ=255)
                                                        aaTAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTgg                                      >  1:1166584/1‑100 (MQ=255)
                                                         aTAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGt                                     <  2:226672/100‑1 (MQ=255)
                                                          tAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGtt                                    >  2:2687406/1‑100 (MQ=255)
                                                            gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCa                                  >  1:796232/1‑100 (MQ=255)
                                                            gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCa                                  >  1:324035/1‑100 (MQ=255)
                                                            gTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCa                                  >  2:1414574/1‑100 (MQ=255)
                                                             tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                                 <  1:2670945/100‑1 (MQ=255)
                                                             tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                                 <  1:809315/100‑1 (MQ=255)
                                                             tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                                 <  2:1035604/100‑1 (MQ=255)
                                                             tACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAc                                 <  2:1297545/100‑1 (MQ=255)
                                                              aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAcc                                <  1:2170632/100‑1 (MQ=255)
                                                              aCCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCAcc                                >  1:1206553/1‑100 (MQ=255)
                                                               ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCGTGCTCTGGTTCACCa                               >  1:2850776/1‑100 (MQ=255)
                                                                  gcagacaaaaaaaTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTc                            >  1:2537835/9‑100 (MQ=37)
                                                                    gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTTTCTTCTTTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCtt                          >  2:86263/1‑99 (MQ=255)
                                                                    gaagtgaaAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTg                          <  1:1329686/100‑1 (MQ=255)
                                                                    agacaaaaaaaTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTg                          >  1:695423/7‑100 (MQ=38)
                                                                       gtgaaAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTgcgc                       >  1:2048925/1‑100 (MQ=255)
                                                                         naaaaaTGGCCCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg                     >  2:2035864/2‑100 (MQ=255)
                                                                         gaaAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg                     >  1:842562/1‑100 (MQ=255)
                                                                         gaaAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg                     <  1:2789554/100‑1 (MQ=255)
                                                                         gaaAAATGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg                     >  2:1538442/1‑100 (MQ=255)
                                                                         aaaaaaTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTg                     >  1:2471094/2‑100 (MQ=255)
                                                                            aaaTGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGAct                  <  1:1322871/100‑1 (MQ=255)
                                                                            aaaTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGAct                  >  1:1715174/1‑100 (MQ=255)
                                                                             aaTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActc                 >  2:712963/1‑100 (MQ=255)
                                                                              aTGGCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActct                >  2:1558345/1‑100 (MQ=255)
                                                                              aTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActct                >  1:1842343/1‑100 (MQ=255)
                                                                              aTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActct                >  1:484946/1‑100 (MQ=255)
                                                                              aTGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGActct                >  1:253384/1‑100 (MQ=255)
                                                                               tGGCGCACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTa               >  1:2667577/1‑100 (MQ=255)
                                                                                ggCGCACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTAc              <  1:861598/100‑1 (MQ=255)
                                                                                 gcgcACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACt             >  1:1271497/1‑100 (MQ=255)
                                                                                 gcgcACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACt             >  2:1304939/1‑100 (MQ=255)
                                                                                  cgcACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTg            >  1:1172247/1‑100 (MQ=255)
                                                                                   gcACATTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGa           <  2:1265553/100‑1 (MQ=255)
                                                                                   gcACAATGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGa           >  1:514578/1‑100 (MQ=255)
                                                                                    cacaTTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGaa          <  1:2451787/100‑1 (MQ=255)
                                                                                    cacaTTGTGCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGaa          >  2:2837488/1‑100 (MQ=255)
                                                                                        ttGTGCGCCATTTTTTTGTCTGACGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGa           >  2:2295295/1‑95 (MQ=255)
                                                                                          gtgCGCCATTTTTTTTTCTTTCTTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGgcgc     >  2:1143520/1‑99 (MQ=255)
                                                                                          gtgCGCCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCa    >  1:1048898/1‑100 (MQ=255)
                                                                                            gcgcCATTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCAtt  >  1:597795/1‑100 (MQ=255)
                                                                                                  |                                                                                              
TGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATT  >  NC_000913/1757600‑1757792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: