Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,533,408 (A)6→5 intergenic (+28/‑356) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,533,4030A.100.0% 438.5 / NA 98intergenic (+23/‑361)cysK/ptsHcysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (38/60);  total (38/60)

Only 100 of 110 total aligned reads displayed.

TACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACT  >  NC_000913/2533304‑2533500
                                                                                                   |                                                                                                 
tACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTa                                                                                                   <  2:1813361/100‑1 (MQ=255)
 aCCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaa                                                                                                  <  1:1755421/100‑1 (MQ=255)
 aCCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaa                                                                                                  <  1:1358643/100‑1 (MQ=255)
  ccATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaaa                                                                                                 >  2:2789624/1‑100 (MQ=255)
  ccATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaaa                                                                                                 <  1:519651/100‑1 (MQ=255)
   catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaaaa                                                                                                <  2:2330334/100‑1 (MQ=255)
       atcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAACGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaaaaagca                                                                                            >  2:1259327/1‑97 (MQ=255)
       atcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTaaaaagca                                                                                            >  1:2612080/1‑97 (MQ=255)
         cGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCAcc                                                                                         <  2:1130283/100‑1 (MQ=255)
          gggTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCt                                                                                        >  2:252958/1‑100 (MQ=255)
           ggTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCtt                                                                                       >  1:874937/1‑100 (MQ=255)
               aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTa                                                                                   >  1:1602340/1‑100 (MQ=255)
               aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTa                                                                                   >  1:708421/1‑100 (MQ=255)
                gCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAg                                                                                  <  1:634520/100‑1 (MQ=255)
                gCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAg                                                                                  <  1:131959/100‑1 (MQ=255)
                gCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAg                                                                                  >  1:2340388/1‑100 (MQ=255)
                 cggtATTTGAGCGCCGCATTGTTTGCCGATCTGGTCGCTGAGAAAGAATTGCAACAGTAATGCCAGGGTGGTAAAAATGCGT‑AAAAAGCACCTTTTTAgg                                                                                 <  2:1821450/97‑1 (MQ=255)
                 cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAgg                                                                                 >  1:57176/1‑100 (MQ=255)
                 cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAgg                                                                                 <  1:2262318/100‑1 (MQ=255)
                 cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAgg                                                                                 <  2:247696/100‑1 (MQ=255)
                  gTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCtaaaaaggt                                                                                >  1:362060/1‑92 (MQ=255)
                   ttATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTg                                                                               <  1:638563/100‑1 (MQ=255)
                     aTTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCt                                                                             >  1:350524/1‑100 (MQ=255)
                        tAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCtttt                                                                          >  1:2050393/1‑100 (MQ=255)
                        tAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTAAAAAGGTGCtttt                                                                          <  2:706236/100‑1 (MQ=255)
                         aaGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCttttt                                                                         <  2:870827/100‑1 (MQ=255)
                         aaGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCttttt                                                                         <  2:719498/100‑1 (MQ=255)
                          aGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCttttta                                                                        <  1:2488385/100‑2 (MQ=255)
                           gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtg                                                                       <  1:915339/100‑1 (MQ=255)
                           gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtg                                                                       >  1:742274/1‑100 (MQ=255)
                           gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTAAAAAGGTGCtttttac                                                                       <  1:861138/100‑3 (MQ=38)
                            cACCGCATTGTTTGTCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtgt                                                                      <  2:2273885/100‑1 (MQ=255)
                            cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtgt                                                                      >  1:534313/1‑100 (MQ=255)
                            cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtgt                                                                      <  2:86344/100‑1 (MQ=255)
                            cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTAAAAAGGTGCtttttacg                                                                      <  1:1087337/100‑4 (MQ=38)
                             aCCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtgtg                                                                     <  1:2357739/100‑1 (MQ=255)
                             aCCGCATTGTTTGCCGAGCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTtg                                                                       <  2:1924410/98‑1 (MQ=255)
                              ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTgg                                                                    >  2:2473289/1‑100 (MQ=255)
                              ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTgg                                                                    >  1:1364298/1‑100 (MQ=255)
                              ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTgg                                                                    <  1:903962/100‑1 (MQ=255)
                              ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTAAAAAGGTGCtttttacg                                                                      <  1:2266810/98‑4 (MQ=38)
                               cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTAAAAAGGTGCtttttacgca                                                                    <  2:2419318/99‑6 (MQ=37)
                                gccttGTTTGCGGATCTGTTGGCTGAGAAAGAATTGAAAGAGTGATGCCAGCTTGTTAGAAATGCGT‑AAAAAGCACCTTTTTAGGTGCttttgttttg                                                                    <  2:1977144/95‑7 (MQ=255)
                                gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGcc                                                                  <  2:121299/100‑1 (MQ=255)
                                gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGcc                                                                  <  1:2240242/100‑1 (MQ=255)
                                gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGcc                                                                  <  2:955349/100‑1 (MQ=255)
                                 cATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCt                                                                 >  2:582171/1‑100 (MQ=255)
                                  aTTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTg                                                                <  1:252959/100‑1 (MQ=255)
                                     gTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAAGGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCt                                                              <  2:21386/99‑1 (MQ=255)
                                      tttGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTc                                                            <  1:1560504/100‑1 (MQ=255)
                                      tttGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTc                                                            <  1:1000261/100‑1 (MQ=255)
                                        tGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCaa                                                          <  1:2725105/100‑1 (MQ=255)
                                          ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAAc                                                        <  1:972919/100‑1 (MQ=255)
                                          ccGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAAc                                                        <  1:2536026/100‑1 (MQ=255)
                                            gATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACtt                                                      >  1:1267681/1‑100 (MQ=255)
                                               ctctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCg                                                   >  2:974230/1‑100 (MQ=255)
                                                 ctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                 <  1:1020699/100‑1 (MQ=255)
                                                 ctTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                 >  2:1239828/1‑100 (MQ=255)
                                                   tCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcccc                                               >  2:328493/1‑100 (MQ=255)
                                                      cTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcc                                            >  2:1492216/1‑100 (MQ=255)
                                                      cTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcc                                            >  2:454804/1‑100 (MQ=255)
                                                       tGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcct                                           >  2:1675244/1‑100 (MQ=255)
                                                       tGAAAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcct                                           <  2:1333771/100‑1 (MQ=255)
                                                        gagaAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTg                                          >  1:370144/1‑100 (MQ=255)
                                                        gagaAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTg                                          >  2:2590978/1‑100 (MQ=255)
                                                          gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGc                                        >  2:499242/1‑100 (MQ=255)
                                                          gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGc                                        >  1:1720976/1‑100 (MQ=255)
                                                          gaaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGc                                        <  2:1009118/100‑1 (MQ=255)
                                                           aaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCa                                       <  2:889436/100‑1 (MQ=255)
                                                           aaagaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCa                                       >  1:2861256/1‑100 (MQ=255)
                                                             agaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTATGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                     <  2:2644692/100‑1 (MQ=255)
                                                             agaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                     >  2:1917721/1‑100 (MQ=255)
                                                             agaaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCAtt                                     <  2:559875/100‑1 (MQ=255)
                                                               aaTTGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGa                                   <  1:2064572/100‑1 (MQ=255)
                                                                  tGCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTc                                >  2:2440934/1‑100 (MQ=255)
                                                                   gCAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCa                               <  2:2365651/100‑1 (MQ=255)
                                                                    ctgaatcaaTGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                              <  1:1419404/93‑1 (MQ=255)
                                                                    cAACAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                              <  1:1157303/100‑1 (MQ=255)
                                                                     aaCAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                             >  1:1811277/1‑100 (MQ=255)
                                                                     aaCAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                             <  1:53935/100‑1 (MQ=255)
                                                                     aaCAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                             <  1:1612937/100‑1 (MQ=255)
                                                                      aCAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGcc                            <  2:2159975/100‑1 (MQ=255)
                                                                       cAGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                           >  2:243664/1‑100 (MQ=255)
                                                                        aGTAATGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTg                          <  2:2519694/100‑1 (MQ=255)
                                                                           aaTGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCg                       <  1:2034331/100‑1 (MQ=255)
                                                                            aTGCCAGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCgg                      <  2:2318250/100‑1 (MQ=255)
                                                                                 aGCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTg                 <  2:1483612/100‑1 (MQ=255)
                                                                                  gCTTGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTgg                >  1:25671/1‑100 (MQ=255)
                                                                                    ttGTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTa              <  2:1986730/100‑1 (MQ=255)
                                                                                      gTTAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt            <  1:384597/100‑1 (MQ=255)
                                                                                        tAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa          >  2:2750643/1‑100 (MQ=255)
                                                                                        tAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa          >  2:2750636/1‑100 (MQ=255)
                                                                                        tAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa          >  1:369017/1‑100 (MQ=255)
                                                                                        tAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa          <  2:733619/100‑1 (MQ=255)
                                                                                        tAAAAATGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTa          >  2:1275197/1‑100 (MQ=255)
                                                                                         aaaaaTGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTaa         <  1:1259332/100‑1 (MQ=255)
                                                                                          aaaaTGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAc        <  2:708419/100‑1 (MQ=255)
                                                                                             aTGCGT‑AAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg     <  1:2005624/100‑1 (MQ=255)
                                                                                                 acaaaaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACt  >  2:1006502/3‑100 (MQ=38)
                                                                                                 acaaaaaaGCACCTAAAAAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACt  >  2:968572/3‑100 (MQ=38)
                                                                                                   |                                                                                                 
TACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACT  >  NC_000913/2533304‑2533500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑