Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,499,708 (C)5→4 intergenic (+93/‑202) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,499,7040C.96.5% 481.2 / 8.7 113intergenic (+89/‑206)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (45/64);  total (47/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTC  >  NC_000913/3499605‑3499799
                                                                                                   |                                                                                               
cTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGc                                                                                                 >  2:2413706/1‑100 (MQ=255)
 tGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGcc                                                                                                >  1:705415/1‑100 (MQ=255)
 tGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGcc                                                                                                <  2:13650/100‑1 (MQ=255)
  gCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccc                                                                                               >  2:673872/1‑100 (MQ=255)
    acaGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccg                                                                                             <  2:2110536/100‑2 (MQ=255)
    acaGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccg                                                                                             <  1:2508199/100‑2 (MQ=255)
       gTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGccccgccc                                                                                          <  1:830022/100‑5 (MQ=255)
          aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTa                                                                                      >  2:2456992/1‑100 (MQ=255)
          aGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTa                                                                                      >  2:2222046/1‑100 (MQ=255)
           gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaa                                                                                     >  1:295896/1‑100 (MQ=255)
           gTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaa                                                                                     <  1:469656/100‑1 (MQ=255)
             ctctGACAGAAATCGGCTAACACCGGTTTAACTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaaaa                                                                                   >  2:388732/1‑100 (MQ=255)
              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaaaaa                                                                                  <  2:206745/100‑1 (MQ=255)
              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaaaaa                                                                                  <  1:741498/100‑1 (MQ=255)
              tctGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTaaaaa                                                                                  >  1:849156/1‑100 (MQ=255)
                 gACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAatat                                                                               >  2:1609491/1‑100 (MQ=255)
                   cAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATAttt                                                                             >  1:1556131/1‑100 (MQ=255)
                   cAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATAttt                                                                             >  1:1461605/1‑100 (MQ=255)
                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTc                                                                           >  1:579070/1‑100 (MQ=255)
                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTc                                                                           >  1:26165/1‑100 (MQ=255)
                     gAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTc                                                                           >  1:613364/1‑100 (MQ=255)
                     gAAATCGACTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTc                                                                           <  1:2231596/100‑1 (MQ=255)
                      aaaTCGGCTACCACCGGTTTAATTGCCCGGCGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTCCCCCGCCCGTAAAAATATTTTc                                                                           <  2:1685775/99‑1 (MQ=255)
                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCAc                                                                         <  2:2695123/100‑1 (MQ=255)
                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCAc                                                                         <  1:1324311/100‑1 (MQ=255)
                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCAc                                                                         <  1:939364/100‑1 (MQ=255)
                       aaTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCAc                                                                         <  2:1230415/100‑1 (MQ=255)
                        aTCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACt                                                                        <  1:2473054/100‑1 (MQ=255)
                           ggCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTa                                                                     <  1:710004/100‑1 (MQ=255)
                           ggCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTa                                                                     <  2:1405552/100‑1 (MQ=255)
                             cTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                   <  2:2170810/100‑1 (MQ=255)
                             cTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTaaa                                                                   >  1:928952/1‑100 (MQ=255)
                              tAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAAt                                                                  <  2:1914423/100‑1 (MQ=255)
                              tAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAAt                                                                  <  1:2567491/100‑1 (MQ=255)
                               aaCACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACttagatc                                                                  >  1:1919637/1‑95 (MQ=255)
                                 cacCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCa                                                               >  1:1642317/1‑100 (MQ=255)
                                  acCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCaa                                                              >  1:11000/1‑100 (MQ=255)
                                   ccGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAt                                                             <  2:187416/100‑1 (MQ=255)
                                    cGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAAtt                                                            >  2:2234483/1‑100 (MQ=255)
                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  2:2808117/100‑1 (MQ=255)
                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  2:1795986/100‑1 (MQ=255)
                                      gTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTg                                                          <  1:1974014/100‑1 (MQ=255)
                                       tttAATTGCCCGACGCCCTCTTGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGa                                                         <  2:2576232/100‑1 (MQ=255)
                                        ttAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGAt                                                        <  1:274919/100‑1 (MQ=255)
                                         tAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATa                                                       <  1:1609501/100‑1 (MQ=255)
                                           aTTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATaan                                                     <  2:1472831/100‑2 (MQ=255)
                                           aTTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAAc                                                     >  1:2879156/1‑100 (MQ=255)
                                            ttttccGACCCCCTCTGGATTTTCCGAGGGCGTATTTATTTCTATGTCATTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:1428082/96‑1 (MQ=255)
                                            ttGCCCGACGTCCTCTGGATTTTCCGAGGGCGTATTTTTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:2300535/100‑1 (MQ=255)
                                            ttGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  2:341810/100‑1 (MQ=255)
                                            ttGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACt                                                    <  1:2828997/100‑1 (MQ=255)
                                             tGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTa                                                   <  2:1637593/100‑1 (MQ=255)
                                              gCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                  <  1:27955/100‑1 (MQ=255)
                                               nccGACGCCCTCTGGATTTTCCGAGGGCGTATTAGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATATTCACTTTAAATTCAATTTGATAACTACa                                                 >  2:1738622/2‑100 (MQ=255)
                                               cccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTAc                                                  >  1:759495/1‑100 (MQ=255)
                                               cccGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACa                                                 >  2:1906320/1‑100 (MQ=255)
                                                 cGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATc                                               >  1:1056650/1‑100 (MQ=255)
                                                      cccTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTa                                          <  1:2298880/100‑1 (MQ=255)
                                                       ccTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAg                                         <  1:1674338/100‑1 (MQ=255)
                                                        ctctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATGACTACATCCATTAGc                                        >  1:2847083/1‑100 (MQ=255)
                                                        ctctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGc                                        <  1:2359918/100‑1 (MQ=255)
                                                         tctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTCCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGcc                                       >  2:1249995/1‑100 (MQ=255)
                                                          ctGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGcc                                       <  2:1355708/99‑1 (MQ=255)
                                                               ttttCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                 >  1:1412401/1‑100 (MQ=255)
                                                               ttttCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGACCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAgcg                                 >  1:2033663/1‑100 (MQ=255)
                                                                   ccGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGAt                             <  2:352852/100‑1 (MQ=255)
                                                                    cGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATc                            >  1:2132196/1‑100 (MQ=255)
                                                                     gAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATcc                           <  2:634640/100‑1 (MQ=255)
                                                                        ggCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCtgt                        <  2:2476552/100‑1 (MQ=255)
                                                                         gCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTGCATCCATTAGCCACAGCGCGATCCtgtg                       <  2:1623173/100‑1 (MQ=255)
                                                                         gCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATcc                           <  2:1232588/96‑1 (MQ=255)
                                                                         gCGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACAGCCATTAGCCACAGCGCGATCCtgt                        <  1:1315825/99‑1 (MQ=255)
                                                                          cGTATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGc                      <  2:613362/100‑1 (MQ=255)
                                                                            tATTTGTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCg                     >  1:1127829/1‑99 (MQ=255)
                                                                                 gTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAAttt               >  1:1857182/1‑100 (MQ=255)
                                                                                 gTTTCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAAttt               >  1:1107295/1‑100 (MQ=255)
                                                                                   ttCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtg             <  1:2707090/100‑1 (MQ=255)
                                                                                   ttCTATGTCCTTCTTGCCCCGCCCGTAAAAATATCTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtg             <  2:1532328/100‑1 (MQ=255)
                                                                                    tCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            >  1:136155/1‑100 (MQ=255)
                                                                                    tCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            <  1:551387/100‑1 (MQ=255)
                                                                                    tCTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            <  1:2527743/100‑1 (MQ=255)
                                                                                     cTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgtg           <  2:1461598/100‑1 (MQ=255)
                                                                                     cTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgt            >  2:2236351/1‑99 (MQ=255)
                                                                                     cTATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACACCCATTAGCCACAGCGCGATCCTGTGCGAAATTTtgtg           <  2:1545580/100‑1 (MQ=255)
                                                                                      tATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:1130484/100‑1 (MQ=255)
                                                                                      tATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:318770/100‑1 (MQ=255)
                                                                                      tATGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGa          <  1:571132/100‑1 (MQ=255)
                                                                                        tGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATc        >  2:2110761/1‑100 (MQ=255)
                                                                                        tGTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATc        <  2:1191005/100‑1 (MQ=255)
                                                                                         gTCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCt       >  1:69377/1‑100 (MQ=255)
                                                                                         gTCCTTCTTGCCCCGCCCGAAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAACAACAGCGCGATCCTGTGCGAAATTTTGTGATCt       >  2:138552/1‑100 (MQ=255)
                                                                                          tCCTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCtt      >  1:2504369/1‑100 (MQ=255)
                                                                                           ccTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc     >  2:1560132/1‑100 (MQ=255)
                                                                                           ccTTCTTGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtc     >  1:1578644/1‑100 (MQ=255)
                                                                                            cttcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcc    <  1:909040/100‑1 (MQ=255)
                                                                                              tcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCACTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  <  2:2033649/100‑1 (MQ=255)
                                                                                              tcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCGTTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:1855520/1‑100 (MQ=255)
                                                                                              tcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:543330/1‑100 (MQ=255)
                                                                                              tcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  1:2765813/1‑100 (MQ=255)
                                                                                              tcttGCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTtcctc  >  2:898905/1‑100 (MQ=255)
                                                                                                   |                                                                                               
CTGCACAGTAAGTCTCTGACAGAAATCGGCTAACACCGGTTTAATTGCCCGACGCCCTCTGGATTTTCCGAGGGCGTATTTGTTTCTATGTCCTTCTTGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTC  >  NC_000913/3499605‑3499799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: