| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 561,696 | (G)6→7 | coding (2000/2604 nt) | sfmD → | putative outer membrane export usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 561,690 | 1 | . | G | 98.6% | 248.7 / ‑3.3 | 71 | coding (1994/2604 nt) | sfmD | putative outer membrane export usher protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (35/35); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
GCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑GGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTG > NC_000913/561594‑561781 | gCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑ggg > 2:1226156/1‑100 (MQ=255)gCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑ggg > 2:2815393/1‑100 (MQ=255) ccACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggg < 1:715806/100‑1 (MQ=255) aCCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcg > 2:1566947/1‑97 (MQ=255) ccTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcgg > 1:2126079/1‑96 (MQ=255) ccTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcg < 2:1367090/99‑4 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcggg < 2:806867/100‑6 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcggg < 1:2354081/100‑6 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcggg > 1:1981739/1‑95 (MQ=255) cTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑gggggggcggg < 1:1207062/100‑6 (MQ=255) tGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGt < 1:1571461/100‑1 (MQ=255) aGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGtat < 2:2807766/100‑1 (MQ=255) aGTGATCACGTAAGCCAGGGTGATACCTCGAACAATGGGTACACGGGCAGCGCCACGGCACACTGGCAGGCCGCTTACGGTACGCT‑GGGGGGCGGGtata < 1:2748061/100‑1 (MQ=255) gTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGtata > 2:1984359/1‑100 (MQ=255) gTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGtata < 2:2865961/100‑1 (MQ=255) gTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGtata < 2:2883850/100‑1 (MQ=255) tATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAAc < 2:735515/100‑1 (MQ=255) tATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAAc < 2:1037176/100‑1 (MQ=255) aTCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACt < 2:1981725/100‑1 (MQ=255) cACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTAc > 2:1936111/1‑100 (MQ=255) cACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTAc > 1:195018/1‑100 (MQ=255) cACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTAc > 1:956180/1‑100 (MQ=255) tAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGAcc < 2:2602414/100‑1 (MQ=255) tAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGAcc > 2:1021289/1‑100 (MQ=255) tAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGAACGCTGGGGGGGCGGGTAGAACTACGAcc > 1:1406344/1‑100 (MQ=255) aaGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACcg < 1:2357251/100‑1 (MQ=255) cAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGAt < 2:940019/100‑1 (MQ=255) cAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGACAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGAt > 2:734864/1‑100 (MQ=255) gggTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCa > 1:2136630/1‑100 (MQ=255) gggTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCa < 2:2872026/100‑1 (MQ=255) gTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAAc > 1:2886912/1‑100 (MQ=255) gATACCTCGAATAATGGGTACACGGGCAGCGCCACGGGAAGCTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATCACTACGACCGCGATCAACa < 2:780163/99‑1 (MQ=255) ccTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACg > 1:1810968/1‑100 (MQ=255) cTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGt > 2:1943622/1‑100 (MQ=255) cTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGt > 1:1294475/1‑100 (MQ=255) cTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGt < 2:550909/100‑1 (MQ=255) cTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGt < 2:1618745/100‑1 (MQ=255) gAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTaa > 2:822024/1‑100 (MQ=255) ataatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACt > 1:43730/1‑100 (MQ=255) ataatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACt > 1:347119/1‑100 (MQ=255) taatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTg < 1:2070790/100‑1 (MQ=255) aatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTgg < 1:2138357/100‑1 (MQ=255) aatGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTgg > 2:315597/1‑100 (MQ=255) atGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGc > 1:875671/1‑100 (MQ=255) atGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGc < 2:3348/100‑1 (MQ=255) atGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGc < 2:2809766/100‑1 (MQ=255) atGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGc < 1:1733679/100‑1 (MQ=255) tGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCa < 2:2868871/100‑1 (MQ=255) tGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCa < 1:1726856/100‑1 (MQ=255) ggttACACGGGCAGCGCCACGGCAAGCTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAg < 2:1721267/97‑1 (MQ=255) ggTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGc > 1:691079/1‑100 (MQ=255) cacGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTc > 1:376888/1‑100 (MQ=255) acGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCt < 1:1049631/100‑1 (MQ=255) acGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCt < 2:2064079/100‑1 (MQ=255) cGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTg > 1:1755567/1‑100 (MQ=255) ggCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGc > 1:567081/1‑100 (MQ=255) ggCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGc < 2:711235/100‑1 (MQ=255) gCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCg > 1:1074108/1‑100 (MQ=255) aGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGgt > 1:786457/1‑100 (MQ=255) gcgcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGgtg > 2:2834741/1‑100 (MQ=255) gcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGtgtg > 2:2199090/1‑100 (MQ=255) gcCACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGtgtg > 1:1422687/1‑100 (MQ=255) gcCACGGCAAACTGGCAGGCCGATTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGtgtg > 2:1677222/1‑100 (MQ=255) cACGGCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGt < 2:379033/100‑1 (MQ=255) ggCAAACTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGCATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCgg > 2:1584704/1‑100 (MQ=255) aaaCTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCa < 1:2822757/100‑1 (MQ=255) aaCTGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCAt < 1:734866/100‑1 (MQ=255) ttccgatctgCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATAAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaa < 1:423325/91‑1 (MQ=255) tGGCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaa < 1:822025/100‑1 (MQ=255) gCAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGaaaa < 2:2057948/100‑1 (MQ=255) cAGGCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGCCGTTAACTGGCAGCTGTCTGGCGGCGTGGTCGGGCATGAAAAt > 2:3923/1‑100 (MQ=255) ggCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATg < 2:1787129/99‑1 (MQ=255) gCCGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGACGGTGTGGTCGGGCATGAAACTgg > 1:844817/1‑99 (MQ=255) ccGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCa > 1:1471108/1‑100 (MQ=255) cGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCAt < 1:1226160/100‑1 (MQ=255) cGCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCAt < 2:2136617/100‑1 (MQ=255) gCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATa > 1:556367/1‑100 (MQ=255) gCTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCGGTCTGGCGGTGTGGTCGGGCATGaaagatcggaa > 2:423324/1‑92 (MQ=255) cTTACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATaa > 1:1238141/1‑100 (MQ=255) ttACGGTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAAc < 2:789449/100‑1 (MQ=255) ggTACGCTGGGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTg > 2:247272/1‑100 (MQ=255) | GCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT‑GGGGGGCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGGCATGAAAATGGCATAACGCTG > NC_000913/561594‑561781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |