| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,611,005 | (C)5→6 | coding (300/759 nt) | hyfI → | hydrogenase 4, Fe‑S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,611,000 | 1 | . | C | 95.7% | 236.4 / 3.7 | 70 | coding (295/759 nt) | hyfI | hydrogenase 4, Fe‑S subunit |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (38/29); total (39/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑CC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCGACAC > NC_000913/2610902‑2611094 | ttAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑c > 2:155578/1‑100 (MQ=255)ttAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑c > 2:2557085/1‑100 (MQ=255) tAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc < 2:1671199/100‑1 (MQ=255) gttgttTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc‑c‑ccc < 1:1941367/100‑2 (MQ=255) gttgttTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc‑c‑ccc > 2:2341586/1‑99 (MQ=255) gttgttTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc‑c‑ccc < 2:1909403/100‑2 (MQ=255) tgttTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc‑c‑cccga < 1:2660231/100‑4 (MQ=255) gttTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑cc‑c‑cccgat < 1:1056387/100‑5 (MQ=255) tcaCCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATaaa > 2:506736/1‑100 (MQ=255) gcgTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAAtttn < 1:2806581/100‑2 (MQ=255) gcgTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTtg < 2:108970/100‑1 (MQ=255) gTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTtgtg < 1:1295469/100‑1 (MQ=255) gTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTtgtg < 2:494395/100‑1 (MQ=255) gTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTtgtg < 1:2557103/100‑1 (MQ=255) gTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTtgtg < 1:2546829/100‑1 (MQ=255) aCGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGttt < 1:2800205/100‑1 (MQ=255) cGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTc < 2:2557505/100‑1 (MQ=255) gCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTcc > 2:293943/1‑100 (MQ=255) ccGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCt > 2:1002951/1‑100 (MQ=255) gATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTAc < 1:2753516/100‑1 (MQ=255) atTTTGTTATNTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGCTCATAAAATTTGTGTTTCCTACGgc > 1:1090390/1‑100 (MQ=255) ttttGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGgcg > 2:624141/1‑100 (MQ=255) ttttGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGgcg < 1:2601652/100‑1 (MQ=255) ttttGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGgcg > 1:2252113/1‑100 (MQ=255) tttGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGgcgc > 1:768096/1‑100 (MQ=255) tttGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGgcgc > 1:1286188/1‑100 (MQ=255) tttttatttatTGGCGCATTGATCCGGGCGATGCTTATGCCTGCACTTCGGGCGTATGAGTTTG‑CCTC‑CCGATCATAAAATTTGTGTTTCCTACGGcgcg < 2:1006137/97‑1 (MQ=255) gTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTg > 1:785161/1‑100 (MQ=255) aTTTACTGGCGCAGTCACCCGGGCGATGGGTATGCGTGTACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCTTACGGCGCGTgggg > 2:1525717/1‑100 (MQ=255) aTTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCgg > 2:889746/1‑100 (MQ=255) aTTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCgg < 2:1077765/100‑1 (MQ=255) tttACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGgt < 1:2425141/100‑1 (MQ=255) aCTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTc > 2:1052122/1‑100 (MQ=255) cTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACATCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcg < 1:2748540/100‑1 (MQ=255) tGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcg > 2:840663/1‑99 (MQ=255) ggCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcggc > 2:796759/1‑100 (MQ=255) ggCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcggc < 2:2772501/100‑1 (MQ=255) gcAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcggcggc < 1:1117936/100‑1 (MQ=255) cAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcggcggcg > 1:120310/1‑100 (MQ=255) aGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTcggcggcgg < 2:2070638/100‑1 (MQ=255) cACCCGGGCGACGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTAt > 2:1516953/1‑100 (MQ=255) cccGGGCGATGCGTATGCCTGCACTTCGGGCGTATGGGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTAttt > 1:2040763/1‑100 (MQ=255) cccGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTAttt > 2:244459/1‑100 (MQ=255) cGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTc > 1:158358/1‑100 (MQ=255) gggCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTcc < 1:293944/100‑1 (MQ=255) gCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCAc > 1:2823979/1‑100 (MQ=255) gCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑CC‑CACCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCAc < 1:1107488/100‑1 (MQ=255) gATGCGAATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGa > 1:1105305/1‑100 (MQ=255) aTGCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGAt < 2:768094/100‑1 (MQ=255) tGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑CC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCa > 2:2231641/1‑94 (MQ=255) gCGTATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGAtct < 1:2370860/100‑1 (MQ=255) tATGCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTa < 1:60028/100‑1 (MQ=255) gCCTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAg > 1:2008579/1‑100 (MQ=255) ccTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGc > 2:1396392/1‑100 (MQ=255) ccTGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGc > 1:703636/1‑100 (MQ=255) tccgatctgggCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGt < 2:1061187/92‑1 (MQ=255) tGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGt > 1:1611261/1‑100 (MQ=255) tGCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGt > 2:1331881/1‑100 (MQ=255) gCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTTCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTc > 2:870634/1‑100 (MQ=255) gCACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTc < 1:440453/100‑1 (MQ=255) cACTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCt < 2:2490094/100‑1 (MQ=255) cTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTgg < 1:145759/100‑1 (MQ=255) cTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTgg > 1:2612998/1‑100 (MQ=255) cTTCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTgg > 2:725700/1‑100 (MQ=255) ttCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTggg < 1:1604430/100‑1 (MQ=255) tCGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGAGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTgggg > 1:865160/1‑100 (MQ=255) cGGGCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGc < 2:967189/100‑1 (MQ=255) gggCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGtagatcgga > 1:1061189/1‑92 (MQ=39) ggCGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCgg < 1:1134463/100‑1 (MQ=255) cGTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTa > 1:1690239/1‑100 (MQ=255) gTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAg > 1:903150/1‑100 (MQ=255) gTATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAg > 1:1424897/1‑100 (MQ=255) tATGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGc > 2:503325/1‑100 (MQ=255) aTGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCg < 1:885698/100‑1 (MQ=255) aTGAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCg < 1:2341601/100‑1 (MQ=255) gAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCGac > 2:2073611/1‑100 (MQ=255) gAGTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCATGCGTGGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCGa > 2:2595182/1‑99 (MQ=255) gTCTGCCC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCGacac > 1:1359549/1‑100 (MQ=255) | TTAAGGTTGTTTCATCACCGCGTCACGCCGATATTTTGTTATTTACTGGCGCAGTCACCCGGGCGATGCGTATGCCTGCACTTCGGGCGTATGAGTCTG‑CC‑C‑CCGATCATAAAATTTGTGTTTCCTACGGCGCGTGCGGTGTCGGCGGCGGTATTTTCCACGATCTCTACAGCGTCTGGGGCGGTAGCGACAC > NC_000913/2610902‑2611094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |