BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,379,266	(C)_7→8	coding (732/1128 nt)	zapE ←	divisome ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,379,259	1	.	C	89.0%	344.4 / 35.4	109	coding (739/1128 nt)	zapE	divisome ATPase
Reads supporting (aligned to +/- strand): ref base . (8/4); new base C (42/55); total (50/59)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c                                                                                                   <  1:2121627/100‑1 (MQ=255)

  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c                                                                                                 >  2:2408960/1‑100 (MQ=255)

   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑c                                                                                               <  1:861851/100‑1 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc                                                                                              <  2:2335092/100‑1 (MQ=255)

    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc                                                                                              >  2:1602397/1‑100 (MQ=255)

         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc‑cc‑ccg                                                                                       <  1:1677173/100‑3 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc‑cc‑ccgcc                                                                                     >  2:2261421/1‑96 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc‑cc‑ccgcc                                                                                     <  2:249918/100‑5 (MQ=255)

           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑c‑c‑cc‑cc‑ccgcc                                                                                     >  1:1940893/1‑96 (MQ=255)

               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGACTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑CGC‑CC‑CC‑CGCCAgcg                                                                                  <  1:1956332/100‑1 (MQ=255)

                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGcc                                                                                <  1:566879/100‑1 (MQ=255)

                  agaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCa                                                                               <  1:2493455/100‑1 (MQ=255)

                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCaa                                                                              >  1:1397724/1‑100 (MQ=255)

                    ncctccAGCGTCGGGTTCACGCCGCCCATTGCTGCTAAGAACCGATGCTAGAGTTCTAACCTCCGTTGATTCTCGCT‑TAC‑C‑C‑C‑CC‑CC‑CGCCAgcg                                                                                  >  2:423981/5‑95 (MQ=255)

                    aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTGGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAAc                                                                             <  1:823512/100‑1 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGAT‑T‑C‑C‑C‑CC‑CC‑CGCCAGCGCCAAcc                                                                            >  2:2165897/1‑100 (MQ=255)

                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAAcc                                                                            >  1:1937222/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCAt                                                                          >  2:1115254/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCAt                                                                          >  2:1033244/1‑100 (MQ=255)

                       gCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCAt                                                                          >  1:1352091/1‑100 (MQ=255)

                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAg                                                                        <  1:2633865/100‑1 (MQ=255)

                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGtt                                                                      <  1:1025096/100‑1 (MQ=255)

                            cGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGttt                                                                     >  2:1224158/1‑100 (MQ=255)

                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCACCCATAGTTTAt                                                                   >  2:1821745/1‑100 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATc                                                                  <  1:2799977/100‑1 (MQ=255)

                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATc                                                                  <  1:518631/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 <  1:1982363/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 <  2:1937208/100‑1 (MQ=255)

                                tGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATcc                                                                 >  2:2345489/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCa                                                                <  2:1873789/100‑1 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCa                                                                >  2:1887850/1‑100 (MQ=255)

                                 ggTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATACa                                                                >  2:1910914/1‑100 (MQ=255)

                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTCTCCAt                                                               >  1:2307026/1‑100 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2040358/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2802344/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:2060394/100‑1 (MQ=255)

                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTg                                                            <  2:617195/100‑1 (MQ=255)

                                      tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCT‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTgc                                                           <  1:1194083/100‑1 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     <  1:30017/100‑1 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     >  1:2860042/1‑100 (MQ=255)

                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                     >  2:2737950/1‑100 (MQ=255)

                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                    >  1:513647/1‑100 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   >  2:1927907/1‑100 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   <  1:2387424/100‑1 (MQ=255)

                                              cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                   >  1:2360604/1‑100 (MQ=255)

                                              atctgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                  <  2:1713797/97‑1 (MQ=255)

                                                ttgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                 <  1:1520012/100‑1 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                >  1:791080/1‑100 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                <  1:809749/100‑1 (MQ=255)

                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                >  1:791474/1‑100 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               <  2:2886244/100‑1 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CCACGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                               >  2:1848791/1‑100 (MQ=255)

                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttagat                                              >  1:1713810/1‑96 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTACCGCCGGTGAATTTTCTCG‑T‑T‑T‑C‑CTCC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             <  2:2307012/100‑1 (MQ=255)

                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                             >  1:616286/1‑100 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:1822082/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:1190089/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                            <  1:1288466/100‑1 (MQ=255)

                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAATTTATCCATTTGCGCCCGGGTTTCATCg                                            >  1:2526687/1‑100 (MQ=255)

                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                           <  2:2569172/100‑1 (MQ=255)

                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                          >  1:656776/1‑100 (MQ=255)

                                                          tGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAg                                       <  1:1375800/100‑1 (MQ=255)

                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  1:827456/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  1:2251817/1‑100 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:760256/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  2:760359/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     <  1:323109/100‑1 (MQ=255)

                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                     >  2:200625/1‑100 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   <  1:1033246/100‑1 (MQ=255)

                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGc                                   >  1:1783607/1‑100 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               >  2:328423/1‑100 (MQ=255)

                                                                  ggTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaaa                               <  1:2019700/100‑1 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  2:1379494/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              >  1:1645035/1‑100 (MQ=255)

                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                              <  1:2480764/100‑1 (MQ=255)

                                                                    ttGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                             <  1:2547731/100‑1 (MQ=255)

                                                                     tGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                            <  1:2062450/100‑1 (MQ=255)

                                                                      gATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCca                           >  1:2094095/1‑100 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         <  2:513932/100‑1 (MQ=255)

                                                                        tttCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcaca                         >  1:363358/1‑100 (MQ=255)

                                                                         ttCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAg                        <  1:1266467/100‑1 (MQ=255)

                                                                          tCTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGa                       >  1:2698461/1‑100 (MQ=255)

                                                                           cTAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                      >  1:2100421/1‑100 (MQ=255)

                                                                            tAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     >  1:1475725/1‑100 (MQ=255)

                                                                            tAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     <  2:642756/100‑1 (MQ=255)

                                                                            tAACGTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                     <  2:2092044/100‑1 (MQ=255)

                                                                                gTCGGTGAATTTTCTCG‑T‑TCC‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  2:2526670/100‑1 (MQ=255)

                                                                                gTCGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                 <  1:715653/100‑1 (MQ=255)

                                                                                  cGGTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCt                <  2:1009494/99‑1 (MQ=255)

                                                                                   ggTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  2:83028/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             >  1:129487/1‑100 (MQ=255)

                                                                                    gTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  2:631434/100‑1 (MQ=255)

                                                                                    gTGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag             <  1:2692988/100‑1 (MQ=255)

                                                                                     tGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            >  2:623663/1‑100 (MQ=255)

                                                                                     tGAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt            >  2:312164/1‑100 (MQ=255)

                                                                                      gAATTTTCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtg           <  2:2880715/100‑1 (MQ=255)

                                                                                         ttttCTCG‑T‑T‑T‑C‑C‑CCACC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTgagtgagt        >  2:692459/1‑100 (MQ=255)

                                                                                           ttCTCG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAgtgt      >  2:1340694/1‑100 (MQ=255)

                                                                                              tcG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg   <  1:2013134/100‑1 (MQ=255)

                                                                                               cG‑T‑T‑TCC‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGc  <  1:169858/100‑1 (MQ=255)

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCG‑T‑T‑T‑C‑C‑CC‑CC‑CGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGC  >  NC_000913/3379160‑3379353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`