Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,224,2420AT25.9% 203.8 / 84.5 116intergenic (+335/+37)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base A (54/32);  new base T (25/5);  total (79/37)
Fisher's exact test for biased strand distribution p-value = 4.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 116 total aligned reads displayed.

GAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCA  >  NC_000913/1224150‑1224339
                                                                                            |                                                                                                 
gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAACACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGcc                                                                                            <  1:537641/100‑1 (MQ=255)
  ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          <  1:785492/100‑1 (MQ=255)
  ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          >  2:2008780/1‑100 (MQ=255)
  ggTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCtt                                                                                          >  1:1758903/1‑100 (MQ=255)
   gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                         <  1:101700/100‑1 (MQ=255)
    tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGa                                                                                        <  2:2248264/100‑1 (MQ=255)
    tGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGa                                                                                        >  1:320453/1‑100 (MQ=255)
     ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                       >  2:924592/1‑100 (MQ=255)
     ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGaa                                                                                       <  1:2391707/100‑1 (MQ=255)
      gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                      <  1:1887655/100‑3 (MQ=255)
      gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                      <  1:532297/100‑1 (MQ=255)
      gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAg                                                                                      <  1:939497/100‑1 (MQ=255)
       aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGa                                                                                     <  1:1281989/100‑1 (MQ=255)
         aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                                   <  1:330693/100‑1 (MQ=255)
            gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                                >  1:2887144/1‑100 (MQ=255)
            gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                                >  2:829189/1‑100 (MQ=255)
             cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAGCGTGGATGAGAGGGATGAAAGACTAAAGGCAGAGAGAACTCTGCCTGGagtttataat                                                                               >  2:1952901/1‑91 (MQ=255)
             cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                               <  1:1638573/100‑1 (MQ=255)
              aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtttttcatc                                                                              <  2:2524935/100‑11 (MQ=37)
              aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc                                                                              <  1:2096687/100‑1 (MQ=255)
                 gaggagTATCAGCAAGAATAGTCGTCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCt                                                                           <  1:2822016/100‑1 (MQ=255)
                      gTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTAc                                                                      <  2:689472/100‑1 (MQ=255)
                      gTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTAc                                                                      >  2:1440154/1‑100 (MQ=255)
                        aTCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg                                                                    >  1:2284420/1‑100 (MQ=255)
                          cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACa                                                                     >  1:2616151/1‑97 (MQ=255)
                          cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                  >  1:2407382/1‑100 (MQ=255)
                          cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                                  >  1:177461/1‑100 (MQ=255)
                           aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                                 >  2:1853267/1‑100 (MQ=255)
                           aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                                 >  2:2080853/1‑100 (MQ=255)
                            gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                                >  1:92018/1‑100 (MQ=255)
                             cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                               <  1:2444178/100‑1 (MQ=255)
                              aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                              >  1:1509580/1‑100 (MQ=255)
                                  aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                          >  2:2683471/1‑100 (MQ=255)
                                    aCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                        >  1:1270494/1‑100 (MQ=255)
                                       cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                     >  1:2804215/1‑100 (MQ=255)
                                       cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                     >  1:2084590/1‑100 (MQ=255)
                                       cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                     >  1:729923/1‑100 (MQ=255)
                                         ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGctc                                                   >  1:1848770/1‑100 (MQ=255)
                                          cgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGctct                                                  <  1:896471/100‑1 (MQ=255)
                                             tttCCCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCtcttct                                               <  1:2706520/100‑1 (MQ=255)
                                              ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCGGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                              <  2:606424/100‑1 (MQ=255)
                                              ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                              <  2:2887123/100‑1 (MQ=255)
                                                aCCACAACGGGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc                                             <  2:874631/99‑1 (MQ=255)
                                                 ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                           >  2:377110/1‑100 (MQ=255)
                                                 ccACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCtt                                           >  1:2741424/1‑100 (MQ=255)
                                                  cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc                                          >  1:2869059/1‑100 (MQ=255)
                                                  cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc                                          >  1:2427724/1‑100 (MQ=255)
                                                  cacaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTc                                          >  2:1314116/1‑100 (MQ=255)
                                                   acaACGTGGATGAGAGGGATGAAAAACTCAAGGGAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTGTTCTGCTTcc                                         >  2:1903020/1‑100 (MQ=255)
                                                   acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                         <  1:2683491/100‑1 (MQ=255)
                                                   acaACGTGGATGAGAGGGATGAAAAACTCAAGACAGAGAGAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                         <  2:2031609/100‑1 (MQ=255)
                                                    caACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCg                                        <  2:71898/100‑1 (MQ=255)
                                                         tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                                   <  2:2082865/100‑1 (MQ=255)
                                                          ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg                                  >  1:2364735/1‑100 (MQ=255)
                                                          ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg                                  <  1:2008794/100‑1 (MQ=255)
                                                           gATGAAAGGGATGAAAAACTCAAGGCAGAGATAACTCTACCATGAAGATAAATGCACTTTTAAAACCGCATTATTTCAGCTCTTATCCTTccagaa                                     >  2:2745687/1‑92 (MQ=255)
                                                               agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                             >  2:690678/1‑100 (MQ=255)
                                                                gagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCaaga                            >  2:2580365/1‑97 (MQ=255)
                                                                 agGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGtt                           >  1:2323215/1‑100 (MQ=255)
                                                                  gggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGCAGATAAATGCGCATTTACAGCGGGCTTATTTCAGCTCTTCTGCTCCCGATACGCTCAc                              >  2:2418630/1‑96 (MQ=255)
                                                                   ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTc                          <  2:2733926/99‑1 (MQ=255)
                                                                   ggATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCa                         >  2:1005337/1‑100 (MQ=255)
                                                                    gATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAg                        >  2:1073118/1‑100 (MQ=255)
                                                                       gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                     >  2:108140/1‑100 (MQ=255)
                                                                       gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                     >  1:710689/1‑100 (MQ=255)
                                                                        aaaaaCTCAATGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCa                    >  2:2036157/1‑100 (MQ=255)
                                                                        aaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCa                    >  1:284657/1‑100 (MQ=255)
                                                                         tatcttCAAGGCAGAGTTATCTCTGCCTTGAGGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTATTCTGCTTCCGGTAAGGTCACGTTCAGCTCaa                   >  1:611507/6‑100 (MQ=37)
                                                                         aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCaa                   >  1:1258070/1‑100 (MQ=255)
                                                                          aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAg                  <  2:2500767/100‑1 (MQ=255)
                                                                          aaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAg                  <  2:1336774/100‑1 (MQ=255)
                                                                           tcttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa                 >  2:5520/4‑100 (MQ=39)
                                                                           aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa                 <  1:1980432/100‑1 (MQ=255)
                                                                            cttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGaa                >  1:2514891/3‑100 (MQ=255)
                                                                             ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               >  2:414842/2‑100 (MQ=255)
                                                                             ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               >  2:1935749/2‑100 (MQ=255)
                                                                             ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               >  2:2319335/2‑100 (MQ=255)
                                                                             ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               >  1:259708/2‑100 (MQ=255)
                                                                             cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt               >  2:1199279/1‑100 (MQ=255)
                                                                              tCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa              >  2:476576/1‑100 (MQ=255)
                                                                              tCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa              >  2:772837/1‑100 (MQ=255)
                                                                              tCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa              >  1:294971/1‑100 (MQ=255)
                                                                              tCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATa              >  1:893702/1‑100 (MQ=255)
                                                                               cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg             >  2:1443637/1‑100 (MQ=255)
                                                                               cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg             >  2:1022410/1‑100 (MQ=255)
                                                                               cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg             >  1:1044225/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg             >  1:2749898/1‑99 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  2:1369999/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  2:1158436/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:2860319/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:1840028/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  1:1471858/1‑100 (MQ=255)
                                                                                aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa            >  2:941663/1‑100 (MQ=255)
                                                                                 aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa           >  2:1959258/1‑100 (MQ=255)
                                                                                 aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa           >  2:683621/1‑100 (MQ=255)
                                                                                 aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa           >  2:1254754/1‑100 (MQ=255)
                                                                                  ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa          >  2:1925187/1‑100 (MQ=255)
                                                                                     agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAatat       >  2:1559819/1‑100 (MQ=255)
                                                                                     agagATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAatat       >  2:918267/1‑100 (MQ=255)
                                                                                          tAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCa  >  2:2151148/1‑100 (MQ=255)
                                                                                            |                                                                                                 
GAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATCGCCA  >  NC_000913/1224150‑1224339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: