Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T24.1% 275.5 / 122.6 116intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (42/46);  new base T (8/20);  total (50/66)
Fisher's exact test for biased strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 128 total aligned reads displayed.

TTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446258‑1446456
                                                                                                    |                                                                                                   
ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                      >  2:1818877/1‑100 (MQ=255)
 tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                    >  2:917617/1‑100 (MQ=255)
 tttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAg                                                                                                    <  2:1809364/100‑1 (MQ=255)
   tCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTa                                                                                                  >  1:2255245/1‑100 (MQ=255)
   tCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTa                                                                                                  <  2:500691/100‑1 (MQ=255)
    ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTan                                                                                                 <  2:2281362/100‑2 (MQ=255)
    ccTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTAc                                                                                                 >  2:788291/1‑100 (MQ=255)
     cTGAAACAAGGTGTATATTCAAAAACTCCTGTCAAATTACCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  2:1789719/1‑100 (MQ=255)
     cTGAAACAAGGTGAATATTCAAAAACTCATGTCAAATTGCCTTTTGCCCTGAAAAATGAATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACa                                                                                                >  1:1779362/1‑100 (MQ=255)
      tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               <  1:1188176/100‑1 (MQ=255)
      tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               >  2:2242280/1‑100 (MQ=255)
       gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACAt                                                                                               >  2:2425124/1‑99 (MQ=255)
       gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              <  2:736195/100‑1 (MQ=255)
       gAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATg                                                                                              >  1:918189/1‑100 (MQ=255)
        aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                             >  2:326268/1‑100 (MQ=255)
        aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                             <  1:14742/100‑1 (MQ=255)
        aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                             <  1:1699220/100‑1 (MQ=255)
        aaaCAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGc                                                                                             <  1:1565032/100‑1 (MQ=255)
           cAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAAc                                                                                          <  2:2199041/100‑1 (MQ=39)
             aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGc                                                                                        <  1:1386220/100‑1 (MQ=255)
              ggTGAATATTCAAAACCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  1:966960/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAGATATCTGTTTTAACTAATTAGCGTTGCAGTACATGCAACGcc                                                                                       >  2:2024603/1‑100 (MQ=255)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  1:2802624/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGcc                                                                                       <  2:1909459/100‑1 (MQ=38)
              ggTGAATATTCAAAAACTCCTGTCAAATTGCCATTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTAAATGCAACGcc                                                                                       >  2:742360/1‑100 (MQ=255)
               gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                      <  2:2760581/100‑1 (MQ=38)
               gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                      <  1:137617/100‑1 (MQ=38)
               gTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCCGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                      <  2:1548633/100‑1 (MQ=38)
                ttaaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTTAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCa                                                                                      <  2:2403640/97‑1 (MQ=37)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCaa                                                                                     <  1:839073/100‑1 (MQ=38)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                     >  2:1618218/1‑100 (MQ=255)
                tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCaa                                                                                     >  2:1488904/1‑100 (MQ=255)
                 gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAt                                                                                    <  2:2775731/100‑1 (MQ=38)
                 gAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAt                                                                                    <  2:1994905/100‑1 (MQ=38)
                 gAATATTAAAAATCTCCTGTCAATTTGCCTTTTGCCCTGAAAAATTCATATTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAt                                                                                    <  2:2582611/100‑1 (MQ=25)
                  aaTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAtt                                                                                   <  1:2188431/100‑1 (MQ=38)
                      ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTACTGCAACGCCAAttagtt                                                                               <  1:2153900/100‑1 (MQ=37)
                      ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                               >  1:1407619/1‑100 (MQ=255)
                      ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAAttagtt                                                                               <  1:320542/100‑1 (MQ=255)
                          aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                           <  1:2302395/100‑1 (MQ=255)
                          aaaaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCg                                                                           <  1:2608960/100‑1 (MQ=255)
                            aaCTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGa                                                                         <  2:1963656/100‑1 (MQ=255)
                                ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAAttt                                                                     >  1:1050963/1‑100 (MQ=255)
                                 cTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTa                                                                    >  2:420219/1‑100 (MQ=255)
                                   gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACCTGCAACGCCAATTAGTTAGCGGAATTTACg                                                                  >  1:400168/1‑100 (MQ=255)
                                   gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                  <  1:19807/100‑1 (MQ=255)
                                   gTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACg                                                                  <  1:645175/100‑1 (MQ=255)
                                    tCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGt                                                                 >  2:1640349/1‑100 (MQ=255)
                                     cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                <  2:226505/100‑1 (MQ=255)
                                     cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                >  1:2374337/1‑100 (MQ=255)
                                      aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCg                                                               <  1:2857297/100‑1 (MQ=255)
                                        aTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGa                                                              >  1:1187315/1‑99 (MQ=255)
                                        aTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGAt                                                             >  2:2662396/1‑100 (MQ=255)
                                         ttGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAAGTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATa                                                            <  1:1368513/100‑1 (MQ=255)
                                             cTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCg                                                        <  1:2869654/100‑1 (MQ=255)
                                               tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGcc                                                      <  1:917619/100‑1 (MQ=255)
                                               tttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGcc                                                      <  1:1679853/100‑1 (MQ=255)
                                                   cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGc                                                  >  1:1164746/1‑100 (MQ=255)
                                                    ccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAGTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                 <  1:2033834/100‑1 (MQ=255)
                                                     cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGt                                                >  1:1058584/1‑100 (MQ=255)
                                                        aaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                             <  2:1104123/100‑1 (MQ=255)
                                                         aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATc                                            >  2:9096/1‑100 (MQ=255)
                                                         aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATc                                            <  1:2059723/100‑1 (MQ=255)
                                                          aaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATcc                                           <  2:2415708/100‑1 (MQ=255)
                                                            aTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCa                                         <  1:1575670/100‑1 (MQ=255)
                                                            aTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCa                                         <  2:214161/100‑1 (MQ=255)
                                                               cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                      >  1:2756072/1‑100 (MQ=255)
                                                               cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                      <  1:1256811/100‑1 (MQ=255)
                                                                  aGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGt                                   >  1:2158829/1‑100 (MQ=255)
                                                                   gTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGtt                                  >  2:1451496/1‑100 (MQ=255)
                                                                     cAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                <  2:2321408/100‑1 (MQ=255)
                                                                     cAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                <  1:2292841/100‑1 (MQ=255)
                                                                     cAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                <  1:420220/100‑1 (MQ=255)
                                                                       aaTATCTGTTTTAACTAATTGGCGTTGCAGTATATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCtt                              >  1:2742089/1‑100 (MQ=255)
                                                                       aaTATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCtt                              <  1:1618228/100‑1 (MQ=255)
                                                                          atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                           <  1:1789732/100‑1 (MQ=255)
                                                                          atCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                           >  1:846858/1‑100 (MQ=255)
                                                                              gctttAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATAcc                       >  1:1955239/3‑100 (MQ=255)
                                                                               ttttAACTAGTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATGTACGTCGATACTCGCCTGGCGTCATCCGAAAGCGTTGCTTAAATACCg                      <  2:661749/100‑1 (MQ=255)
                                                                                  tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   <  1:1309938/100‑1 (MQ=255)
                                                                                  tAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                   <  2:2356787/100‑1 (MQ=255)
                                                                                     cTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaa                >  1:778708/1‑100 (MQ=255)
                                                                                      tAATTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaa               >  1:946944/1‑100 (MQ=38)
                                                                                       aaTTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa              <  1:1488911/100‑1 (MQ=255)
                                                                                        aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             >  1:1020877/1‑100 (MQ=38)
                                                                                        aTTGGCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt             >  1:2348523/1‑100 (MQ=38)
                                                                                          tGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGa           >  2:1186112/1‑100 (MQ=255)
                                                                                           ggCGTTGCATGTACTGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:765130/1‑100 (MQ=38)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          >  2:2321457/1‑100 (MQ=255)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          <  1:237406/100‑1 (MQ=255)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAc          <  1:1640361/100‑1 (MQ=255)
                                                                                           ggCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCCTTGCTTAAATACCGTTGAAAAATGAc          <  1:1764026/100‑1 (MQ=255)
                                                                                              gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct       >  1:1504318/1‑100 (MQ=255)
                                                                                              gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct       >  1:2761420/1‑100 (MQ=255)
                                                                                              gTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGActct       >  1:424519/1‑100 (MQ=255)
                                                                                                  cAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTc   >  2:1266813/1‑100 (MQ=255)
                                                                                                   aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  2:1451050/1‑100 (MQ=255)
                                                                                                   aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  2:10285/1‑100 (MQ=255)
                                                                                                   aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  >  1:2109244/1‑100 (MQ=255)
                                                                                                   aGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa  <  1:1718192/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCAGTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCA  >  NC_000913/1446258‑1446456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: