Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,183,3451.T22.5% 350.5 / 139.6 142intergenic (+22/+468)zupT/ribBzinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (61/49);  new base T (20/12);  total (81/61)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.65e-01

Only 100 of 151 total aligned reads displayed.

CCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183246‑3183444
                                                                                                    |                                                                                                   
cccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCa                                                                                                      >  1:371703/1‑100 (MQ=255)
cccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCa                                                                                                      >  1:2055859/1‑100 (MQ=255)
 ccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAg                                                                                                    <  1:426869/100‑1 (MQ=255)
 ccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAg                                                                                                    >  1:2433932/1‑100 (MQ=255)
 ccAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAg                                                                                                    <  1:234528/100‑1 (MQ=255)
      tATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCAt                                                                                               <  2:1928728/100‑1 (MQ=255)
        tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                             >  1:1083817/1‑100 (MQ=255)
        tGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATgg                                                                                             <  2:443333/100‑1 (MQ=255)
          gCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGAc                                                                                           >  2:1732330/1‑100 (MQ=255)
             tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                        <  2:2569896/100‑1 (MQ=39)
             tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                        <  2:1044486/100‑1 (MQ=39)
             tACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAAc                                                                                        <  2:2102903/100‑1 (MQ=255)
             atcTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAc                                                                                        <  1:1004638/98‑1 (MQ=39)
                 aTGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAAcagat                                                                                    >  2:1004636/1‑96 (MQ=38)
                  tgtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTg                                                                                   <  1:1302723/100‑1 (MQ=255)
                   gtgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGc                                                                                  <  1:131631/100‑1 (MQ=255)
                    tgGAATGTCAGTGGTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGcc                                                                                 <  2:1497998/100‑1 (MQ=255)
                    tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgat                                                                                 <  1:2418145/100‑3 (MQ=38)
                    tgGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGcc                                                                                 <  2:556018/100‑1 (MQ=255)
                       aaTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCATGCGCTGGACAACTTCTgatgtt                                                                              <  2:2827270/100‑6 (MQ=37)
                       aaTGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGc                                                                              <  1:2045303/100‑1 (MQ=255)
                         tGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCtt                                                                            >  2:934672/1‑100 (MQ=255)
                          gTCAGTGATGGGATTCAGGTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTc                                                                           <  2:180643/100‑1 (MQ=255)
                           tCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCa                                                                          >  2:188677/1‑100 (MQ=255)
                                gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                     >  2:1500648/1‑100 (MQ=255)
                                gATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGaaa                                                                     >  2:2879023/1‑100 (MQ=255)
                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                    <  2:909175/100‑1 (MQ=255)
                                 aTGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAg                                                                    >  1:1230341/1‑100 (MQ=255)
                                      aTTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTa                                                               >  2:608713/1‑100 (MQ=255)
                                      aTTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTa                                                               >  2:1989613/1‑100 (MQ=255)
                                        tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                             <  2:645845/100‑1 (MQ=255)
                                        tCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTAtt                                                             <  2:2727455/100‑1 (MQ=255)
                                         cAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            >  2:2654670/1‑100 (MQ=255)
                                          aGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTc                                                            <  2:437690/99‑1 (MQ=255)
                                          aGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCt                                                           >  2:2646615/1‑100 (MQ=255)
                                           gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                          >  2:2500141/1‑100 (MQ=255)
                                           gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                          >  2:759080/1‑100 (MQ=255)
                                           gTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTa                                                          <  1:595735/100‑1 (MQ=255)
                                            tttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAc                                                         <  1:379017/100‑1 (MQ=255)
                                             ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  1:1902269/100‑1 (MQ=255)
                                             ttAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAcc                                                        <  1:2410955/100‑1 (MQ=255)
                                               aGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCtt                                                      >  1:685186/1‑100 (MQ=255)
                                                gTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCttt                                                     >  1:1980735/1‑100 (MQ=255)
                                                gTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCttt                                                     >  2:2580997/1‑100 (MQ=255)
                                                 tgctgcTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCtttt                                                    >  2:1770390/1‑100 (MQ=255)
                                                  gctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTc                                                   >  2:84624/1‑100 (MQ=255)
                                                   ctgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCg                                                  <  2:2848592/100‑1 (MQ=255)
                                                    tgctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCgg                                                 >  2:1340875/1‑100 (MQ=255)
                                                     gctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGc                                                >  1:2156221/1‑100 (MQ=255)
                                                      ctACAAACGGCGGGAATTGGTTAAAAACAACATCAGAACTTGTCCAGCGCATGGACAATTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCt                                               <  2:1583525/100‑1 (MQ=255)
                                                       tACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTg                                              <  2:305887/100‑1 (MQ=255)
                                                        aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                             >  1:100359/1‑100 (MQ=255)
                                                        aCAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGt                                             >  1:1119200/1‑100 (MQ=255)
                                                         cAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtc                                            <  2:1119828/100‑1 (MQ=255)
                                                           aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTAGCTTTTCGGCTGtctc                                          >  1:2846566/1‑100 (MQ=255)
                                                           aaCGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctc                                          >  2:1329585/1‑100 (MQ=255)
                                                              ggcggGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGtctcttc                                       <  2:634058/100‑1 (MQ=255)
                                                                      ttGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTAc                               <  2:98073/100‑1 (MQ=255)
                                                                        ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                             >  1:1423091/1‑100 (MQ=255)
                                                                        ggTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACCAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTg                             >  2:1851627/1‑100 (MQ=255)
                                                                         gTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGt                            >  2:2192746/1‑100 (MQ=255)
                                                                          ttAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGtt                           >  1:334834/1‑100 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          >  2:2793477/1‑100 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          <  1:429396/100‑1 (MQ=255)
                                                                           tAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGttt                          <  2:1402330/100‑1 (MQ=255)
                                                                            aaaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTa                         <  1:2853870/100‑1 (MQ=255)
                                                                            aaaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTa                         <  1:934674/100‑1 (MQ=255)
                                                                            aaaaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTa                         <  1:1965435/100‑1 (MQ=255)
                                                                              aaaCAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAg                       <  1:949579/100‑1 (MQ=255)
                                                                                acaacaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTa                     >  1:1421884/1‑100 (MQ=255)
                                                                                   ctggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                  >  1:1359020/5‑100 (MQ=37)
                                                                                   acaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                  <  1:644992/100‑1 (MQ=255)
                                                                                   acaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                  <  1:1878264/100‑1 (MQ=255)
                                                                                   acaTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTATACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttt                  >  1:2216793/1‑100 (MQ=255)
                                                                                    caTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTGCTGTTTAAGTATTTg                 <  1:663460/100‑1 (MQ=255)
                                                                                     ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:1251550/3‑100 (MQ=38)
                                                                                     ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:278974/3‑100 (MQ=38)
                                                                                     ggcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  1:893870/3‑100 (MQ=38)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTCTTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTAttttt                >  1:2582911/1‑98 (MQ=255)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  2:528356/1‑100 (MQ=255)
                                                                                     aTCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGt                >  2:1661188/1‑100 (MQ=255)
                                                                                      gcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGtt               >  1:381380/2‑100 (MQ=38)
                                                                                      gcAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGtt               >  1:2580033/2‑100 (MQ=38)
                                                                                       cAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc              >  1:2713398/1‑100 (MQ=38)
                                                                                       cAGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTc              >  2:2638555/1‑100 (MQ=38)
                                                                                        aGAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             >  1:521937/1‑100 (MQ=38)
                                                                                        aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             >  2:1328134/1‑100 (MQ=255)
                                                                                        aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             <  2:2513369/100‑1 (MQ=255)
                                                                                        aGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTcc             <  1:73650/100‑1 (MQ=255)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:2480798/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:2271413/1‑100 (MQ=39)
                                                                                         gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  1:1342843/1‑100 (MQ=255)
                                                                                         gAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcg            >  2:919319/1‑100 (MQ=255)
                                                                                           aGTTGTCCATGCGCTGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCcgcg          >  2:2470011/1‑100 (MQ=39)
                                                                                            gTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCgcgc         >  1:2500890/1‑100 (MQ=255)
                                                                                                tCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCa     >  2:1885018/1‑100 (MQ=255)
                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCaa    >  1:635987/1‑100 (MQ=255)
                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCaa    >  2:2699112/1‑100 (MQ=255)
                                                                                                 ccAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTACGCGCTTc      >  1:1685724/1‑98 (MQ=255)
                                                                                                   aGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGc  <  1:1732343/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
CCCAGCTATGGCGTACTATGTGGAATGTCAGTGATGGGATTCAGTTTAGTGCTGCTACAAACGGCGGGAATTGGTTAAAAACAACATCAGAAGTTGTCCAGCGCATGGACAACTTCTGCCAGCTTCACGAAAGCCTTATTCTACCTTTTCGGCTGTCTCTTCTCTCGTACTGTTTAAGTATTTGTTCCGCGCTTCAAGC  >  NC_000913/3183246‑3183444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: