Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9700GA36.4% 273.0 / 199.8 195intergenic (‑180/+128)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (63/61);  new base A (70/1);  total (133/62)
Fisher's exact test for biased strand distribution p-value = 2.77e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.54e-01

Only 100 of 205 total aligned reads displayed.

CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069
                                                                                                   |                                                                                                          
cAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                            >  1:2457955/1‑100 (MQ=255)
cAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATg                                                                                                            <  1:2553735/100‑1 (MQ=255)
 aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCGTTGTTTATGCCGGATgc                                                                                                           >  2:2507133/1‑100 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                                                           >  1:1000697/1‑99 (MQ=255)
  gggAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTATTTATGCCGGATgcg                                                                                                          >  1:2841906/1‑100 (MQ=255)
       tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                     >  1:2204376/1‑100 (MQ=255)
       tGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                                                                                     <  1:2095611/100‑1 (MQ=255)
         naGTATTGCTGCCGGATGGCGCTGCACGCAGAGGNACANCGCCNCCNGGTANACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                   >  1:1402848/2‑100 (MQ=255)
         aaGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTaa                                                                                                   >  1:152175/1‑100 (MQ=255)
          aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                  >  2:1916493/1‑100 (MQ=255)
             aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                               >  1:1151360/1‑100 (MQ=255)
             aTTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGcc                                                                                               <  1:1602278/100‑1 (MQ=255)
              ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                              <  1:1981048/100‑1 (MQ=255)
                gctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                            >  1:2184057/1‑100 (MQ=255)
                 ctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTAt                                                                                           <  2:944258/100‑1 (MQ=255)
                  tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                          <  2:1570983/100‑1 (MQ=255)
                   gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                         >  1:1875217/1‑100 (MQ=255)
                    cctgATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCg                                                                                        <  1:57057/97‑1 (MQ=255)
                      ggATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGc                                                                                      <  2:2049852/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                     <  1:202900/100‑1 (MQ=255)
                       gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                     <  2:697841/100‑2 (MQ=37)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                    <  1:2802245/100‑1 (MQ=255)
                        aTGGCGCTGCACGCAGAGGCACAGCGCCACCCGGTATTCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt                                                                                    <  2:1954071/100‑1 (MQ=255)
                            cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                               <  1:2191731/100‑1 (MQ=255)
                             gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                              >  1:1808665/1‑100 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          <  1:270829/100‑1 (MQ=255)
                                 cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          <  2:354577/100‑1 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      <  1:1726175/100‑1 (MQ=255)
                                     cAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa                                                                      <  2:522884/100‑1 (MQ=255)
                                        agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                   <  2:411961/100‑1 (MQ=255)
                                           cacaGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCa                                                                >  1:181582/1‑100 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              >  1:1057510/1‑100 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  2:1877781/100‑1 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  1:2364772/100‑1 (MQ=255)
                                             caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  1:394598/100‑1 (MQ=255)
                                              aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGTCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                             <  2:2704773/100‑1 (MQ=255)
                                                   cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                        >  1:1263404/1‑100 (MQ=255)
                                                    aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                       <  1:66680/100‑1 (MQ=255)
                                                      cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑Ag                                                   >  2:1185729/1‑100 (MQ=255)
                                                         gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAt                                               <  2:233905/100‑1 (MQ=255)
                                                          tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAtt                                             <  2:2457939/100‑1 (MQ=255)
                                                           ataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAttt                                            >  2:2602900/1‑100 (MQ=255)
                                                            taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAAtttt                                           >  1:421559/1‑100 (MQ=255)
                                                             aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTc                                          >  2:1407566/1‑100 (MQ=255)
                                                             aCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTc                                          <  2:1263636/100‑1 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                       <  1:2569771/100‑1 (MQ=255)
                                                              cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑g                                       >  1:2166583/1‑100 (MQ=255)
                                                                  ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAgg                                   <  2:2125858/100‑1 (MQ=255)
                                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAgg                                   >  2:97915/7‑100 (MQ=18)
                                                                  gcataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAgg                                   >  1:1916155/7‑100 (MQ=18)
                                                                   tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGc                                  <  1:996075/100‑1 (MQ=255)
                                                                        aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGAt                             >  2:764674/1‑100 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATa                            >  1:371195/1‑100 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATa                            <  2:477514/100‑1 (MQ=255)
                                                                         cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATa                            >  1:1630597/1‑100 (MQ=255)
                                                                              tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCgg                       <  2:654336/100‑1 (MQ=255)
                                                                               gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                      <  1:2240555/100‑1 (MQ=255)
                                                                               gcgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGa                      <  2:1482778/100‑1 (MQ=255)
                                                                                cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAg                     <  1:1779697/100‑1 (MQ=255)
                                                                                  gcttttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAgcg                   >  2:2197671/11‑100 (MQ=17)
                                                                                   tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAgcgc                  <  2:73948/100‑1 (MQ=255)
                                                                                   gttttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGC‑‑AGGAAACAC‑‑GTAGGCCTGATAAGCGAAgcgc                  >  1:2581205/10‑100 (MQ=18)
                                                                                    ttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                 <  2:2571594/100‑1 (MQ=255)
                                                                                      ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa              >  1:1026383/7‑100 (MQ=18)
                                                                                      ttgtaggCCGGATAAGGCGTTCGCGCCGCATCCGGCATACAAAAGTTTGCAAATTCAATAAATTGC‑‑AGAATGCAT‑‑GTAGGCCTGATAAGCGAAGCACATc               >  2:2452989/7‑100 (MQ=12)
                                                                                      gTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCACTACATTGC‑‑AGCATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCagc               <  1:1951477/100‑3 (MQ=255)
                                                                                       tttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCa              >  1:2072212/1‑100 (MQ=255)
                                                                                       tgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGC‑‑AATATGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  1:747543/6‑100 (MQ=18)
                                                                                        ttATGCCGGATGCGGCGTTAACGCCGTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg             <  2:500621/100‑1 (MQ=255)
                                                                                        gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg             >  2:2422358/5‑100 (MQ=21)
                                                                                         taggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGC‑‑AATCTTCTG‑‑GTAGGCCTGATAAGCATCGCGCATCAgg            >  1:1280926/4‑100 (MQ=18)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGT‑‑GGGCTCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg            >  1:301902/4‑100 (MQ=21)
                                                                                         taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  1:356265/4‑100 (MQ=21)
                                                                                          nggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGC‑‑‑‑AATTCTCTTGTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:1712541/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc           >  1:217107/3‑100 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc           >  1:2344368/3‑100 (MQ=25)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGc           >  2:1784239/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:2127215/3‑100 (MQ=21)
                                                                                          aggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  2:2019591/3‑100 (MQ=18)
                                                                                          aggCCGGATAAGGCGTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAACTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGc           >  2:1243322/3‑100 (MQ=17)
                                                                                          aggCCGGATAAGGCGGTCACGCCGCATCCGGCCTACGAAACCGCAAGAATTCAATATATTGC‑‑AGGAGCGGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc           >  2:1635350/3‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGC‑‑GAATCCCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  1:2230712/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:2830079/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:1065231/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGC‑‑AGGACCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:2647303/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa          >  1:2689107/2‑100 (MQ=25)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGC‑‑AGTTTATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa          >  2:1327292/2‑100 (MQ=21)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATTGTGTAAATTCAATAAATTGA‑‑TCGATCCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:1028915/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCACGCAAACTCAATATATTGC‑‑AGAGATCAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  1:1217599/2‑100 (MQ=17)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGC‑‑AGGATACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa          >  2:600718/2‑100 (MQ=18)
                                                                                           ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGC‑‑AGGAGTTAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt         >  1:2828885/2‑100 (MQ=21)
                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGC‑‑AGAGTTCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCag         >  1:806694/1‑99 (MQ=17)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGC‑‑ACCATCCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa         >  1:1572285/1‑99 (MQ=21)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGC‑‑AGGAAACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCag         >  1:879250/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACCTTGCTAATTCAATATATTGC‑‑AGGGACTAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCag         >  2:2866730/1‑99 (MQ=18)
                                                                                            gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAACCGCGCAAATTCAATATATTGCGGAGAAAAT‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGctgt        >  2:2659404/1‑97 (MQ=21)
                                                                                             ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAACCGCGCAAATTCAATATATTGCGGAGAAAAT‑‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGctgtt       >  1:477658/1‑96 (MQ=21)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  2:2841885/100‑1 (MQ=255)
                                                                                                 aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg    <  2:806011/100‑1 (MQ=255)
                                                                                                   gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc  <  2:2307376/100‑1 (MQ=255)
                                                                                                   |                                                                                                          
CAGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGC‑‑AGAATTTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCC  >  NC_000913/3705871‑3706069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: