Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 55,620 A→G M497T (ATG→ACG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091355,6200AG97.2% 375.2 / ‑0.6 109M497T (ATG→ACG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (58/48);  total (60/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 111 total aligned reads displayed.

GAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACC  >  NC_000913/55521‑55716
                                                                                                   |                                                                                                
gAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCa                                                                                                  <  1:1753501/100‑1 (MQ=255)
  gTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACTTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCgta                                                                                                <  1:2293607/100‑1 (MQ=255)
    cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATc                                                                                              >  1:780206/1‑100 (MQ=255)
    cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATc                                                                                              <  1:2664839/100‑1 (MQ=255)
     gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATcg                                                                                             >  1:8477/1‑100 (MQ=255)
      tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATcgc                                                                                            >  2:1099003/1‑100 (MQ=255)
          tataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGGGCGCTCGGGTCCATCGTTTGGTTATAACCCTGAGCCAGCATTTCCGTATCGCGTTc                                                                                        >  1:854386/1‑100 (MQ=255)
          tataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGttt                                                                                        <  1:2231202/100‑2 (MQ=255)
          tataGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTc                                                                                        >  2:597711/1‑100 (MQ=255)
             aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCaaa                                                                                     >  1:1100808/1‑100 (MQ=255)
              gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAg                                                                                    >  1:2493259/1‑100 (MQ=255)
                tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGAc                                                                                  <  2:955129/100‑1 (MQ=255)
                tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGAc                                                                                  >  1:225752/1‑100 (MQ=255)
                atggtctttGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGAc                                                                                  <  1:1316911/92‑1 (MQ=255)
                    gCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAtt                                                                              >  1:821646/1‑100 (MQ=255)
                    gCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAtt                                                                              >  1:2013609/1‑100 (MQ=255)
                    gCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAtt                                                                              >  2:2382393/1‑100 (MQ=255)
                     ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAtt                                                                              >  2:2874507/1‑99 (MQ=255)
                         naTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:1924726/2‑100 (MQ=255)
                         gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCTGTATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:1700541/1‑100 (MQ=255)
                         gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGcc                                                                         <  1:1765404/100‑1 (MQ=255)
                         gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:1423080/1‑100 (MQ=255)
                          aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCg                                                                        <  2:2528859/100‑1 (MQ=255)
                           tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGt                                                                       >  2:1736993/1‑100 (MQ=255)
                              cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                    >  2:1922107/1‑100 (MQ=255)
                              cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                    >  2:2300177/1‑100 (MQ=255)
                              cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATAGCGTTCAAAGACCATTTTGCCGTCaa                                                                    >  2:1935739/1‑100 (MQ=255)
                                  aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACttt                                                                <  1:1716322/100‑1 (MQ=255)
                                   tctcACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTg                                                               <  2:1449659/97‑1 (MQ=255)
                                   cGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTg                                                               <  1:443252/100‑1 (MQ=255)
                                    ggCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGa                                                              >  1:690264/1‑100 (MQ=255)
                                    ggCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGa                                                              >  2:2355766/1‑100 (MQ=255)
                                     gCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGTAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGaa                                                             <  1:2321319/100‑1 (MQ=255)
                                      cACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGaga                                                            >  1:1449666/1‑98 (MQ=255)
                                      cACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAt                                                            <  1:1853234/100‑1 (MQ=255)
                                      cACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAt                                                            >  2:297680/1‑100 (MQ=255)
                                      cACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGAATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAt                                                            <  2:2790535/100‑1 (MQ=255)
                                         gTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGc                                                         >  1:917667/1‑100 (MQ=255)
                                          tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                        <  1:1281660/100‑1 (MQ=255)
                                          tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                        >  1:1693933/1‑100 (MQ=255)
                                          tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                        >  2:1342416/1‑100 (MQ=255)
                                          tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                        >  1:1239370/1‑100 (MQ=255)
                                           aCAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                       >  1:2733899/1‑100 (MQ=255)
                                           aCAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTCCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                       <  1:1928383/100‑1 (MQ=255)
                                           aCAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCgg                                                       <  1:2199974/100‑1 (MQ=255)
                                            cAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGc                                                      >  2:2754127/1‑100 (MQ=255)
                                             aaaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCa                                                     <  1:1099006/100‑1 (MQ=255)
                                             aaaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCa                                                     <  2:1381964/100‑1 (MQ=255)
                                              aaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                    <  1:2604748/100‑1 (MQ=255)
                                              aaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                    <  2:2840013/100‑1 (MQ=255)
                                                    gcgcgcgcGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcgcg                                              >  2:2544266/1‑100 (MQ=255)
                                                     cgcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                             <  2:766951/100‑1 (MQ=255)
                                                     cgcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                             <  1:2504465/100‑1 (MQ=255)
                                                     cgcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                             <  2:924917/100‑1 (MQ=255)
                                                      gcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGt                                            <  2:1061737/100‑1 (MQ=255)
                                                       ctcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGtt                                           >  1:1953290/3‑100 (MQ=255)
                                                       cgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGtt                                           >  2:362297/1‑100 (MQ=255)
                                                          gcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAAc                                        <  1:1236235/100‑1 (MQ=255)
                                                            ggTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACgg                                      <  2:816747/100‑1 (MQ=255)
                                                            ggTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACgg                                      <  1:765371/100‑1 (MQ=255)
                                                            ggTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACg                                       <  2:2331007/99‑1 (MQ=255)
                                                               tCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                   >  1:1255659/1‑100 (MQ=255)
                                                               tCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                   <  1:33309/100‑1 (MQ=255)
                                                               tCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                   >  2:1184289/1‑100 (MQ=255)
                                                                ccAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTc                                  <  2:1910750/100‑1 (MQ=255)
                                                                  aGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGt                                >  1:2561779/1‑100 (MQ=255)
                                                                    cGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTcc                              <  2:225751/100‑1 (MQ=255)
                                                                     gTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                             >  2:2011183/1‑100 (MQ=255)
                                                                      tttGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCan                            <  1:1924740/100‑2 (MQ=255)
                                                                         gggTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCtt                         <  1:1342421/100‑1 (MQ=255)
                                                                          ggTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTg                        >  1:624832/1‑100 (MQ=255)
                                                                          ggTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTg                        >  1:2302920/1‑100 (MQ=255)
                                                                            tataACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                      <  1:2382408/100‑1 (MQ=255)
                                                                            tataACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGt                      >  1:1173891/1‑100 (MQ=255)
                                                                            tataACCCGGAGCCAGCATTTCCGTATCGCGTTAAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCACGCTTGGt                      >  2:2463463/1‑100 (MQ=255)
                                                                             ataACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTc                     >  1:2280580/1‑100 (MQ=255)
                                                                              taACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                    <  1:1737006/100‑1 (MQ=255)
                                                                                aCCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTATGCGGTTAACGGATTCGTGCAGCTTGGTCatg                  <  1:1943540/100‑1 (MQ=255)
                                                                                aCCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtg                  <  1:2300190/100‑1 (MQ=255)
                                                                                 cccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                 >  1:1679289/1‑100 (MQ=255)
                                                                                 cccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                 >  2:2827432/1‑100 (MQ=255)
                                                                                 cccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                 >  2:1835034/1‑100 (MQ=255)
                                                                                 cccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                 <  2:359947/100‑1 (MQ=255)
                                                                                    ggAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc              <  2:821645/100‑1 (MQ=255)
                                                                                    ggAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTc              <  1:1423086/100‑1 (MQ=255)
                                                                                     gAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCt             <  1:75700/100‑1 (MQ=255)
                                                                                       gCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg           >  1:2052589/1‑100 (MQ=255)
                                                                                       gCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg           >  1:2050896/1‑100 (MQ=255)
                                                                                         cAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg         >  2:2801556/1‑100 (MQ=255)
                                                                                         cAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg         <  2:780205/100‑1 (MQ=255)
                                                                                         cAGCATTTCCGTATCGCGTTCAAAGAACATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg         >  1:1759091/1‑100 (MQ=255)
                                                                                          aGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        <  1:2827453/100‑1 (MQ=255)
                                                                                          aGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        <  2:1255654/100‑1 (MQ=255)
                                                                                            cATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta      <  2:1157568/100‑1 (MQ=255)
                                                                                             aTTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCTACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtat     >  1:1479132/1‑100 (MQ=255)
                                                                                              tttCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTtata    >  1:202320/1‑100 (MQ=255)
                                                                                              tttCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGACATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCACGAGTtata    >  2:228314/1‑100 (MQ=255)
                                                                                                tCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc  >  2:667421/1‑100 (MQ=255)
                                                                                                tCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATAcc  >  2:529981/1‑100 (MQ=255)
                                                                                                tCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGGTTCTGGAGTTATAcc  >  2:1728136/1‑100 (MQ=255)
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GAGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACC  >  NC_000913/55521‑55716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑