Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,671,943 G→C intergenic (‑49/‑471) yhjB ← / → yhjC putative DNA‑binding transcriptional response regulator/LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,671,9430GC97.8% 477.3 / ‑4.0 135intergenic (‑49/‑471)yhjB/yhjCputative DNA‑binding transcriptional response regulator/LysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base C (73/59);  total (74/61)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGGTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGTTACA  >  NC_000913/3671846‑3672038
                                                                                                 |                                                                                               
tCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTa                                                                                               <  2:119916/100‑1 (MQ=255)
tCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTa                                                                                               <  1:660130/100‑1 (MQ=255)
  aTTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTAtt                                                                                             >  1:381023/1‑100 (MQ=255)
  aTTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTAtt                                                                                             >  2:2613440/1‑100 (MQ=255)
  aTTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTAtt                                                                                             >  1:2294777/1‑100 (MQ=255)
    tCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAc                                                                                           >  2:1024659/1‑100 (MQ=255)
     ccATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAcc                                                                                          <  1:2441432/100‑1 (MQ=255)
     ccATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAcc                                                                                          >  1:1848035/1‑100 (MQ=255)
     ccATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAc                                                                                           >  2:548845/1‑99 (MQ=255)
      cATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAccc                                                                                         >  1:150569/1‑100 (MQ=255)
       aTGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCcg                                                                                        <  2:209913/100‑1 (MQ=255)
        tGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCcgc                                                                                       <  2:1470223/100‑1 (MQ=255)
        tGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCcgc                                                                                       >  1:1917017/1‑100 (MQ=255)
        tGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCcgc                                                                                       >  1:1451173/1‑100 (MQ=255)
         gAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCcgcg                                                                                      <  1:614407/100‑1 (MQ=255)
           ataaataTTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCgag                                                                                    <  2:2146543/100‑1 (MQ=255)
            taaataTTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAGTACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCgaga                                                                                   <  1:1150631/100‑1 (MQ=255)
            taaataTTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCgaga                                                                                   >  2:1440931/1‑100 (MQ=255)
              aataTTGACTGCCTGTCAAACATGACTATTTGCATAGCTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGaaa                                                                                 >  1:1849391/1‑100 (MQ=255)
              aataTTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGaaa                                                                                 <  2:1566522/100‑1 (MQ=255)
              aataTTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGaaa                                                                                 <  2:1848022/100‑1 (MQ=255)
                tatTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGaaaaa                                                                               >  2:2775990/1‑100 (MQ=255)
                 atTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGaaaaaa                                                                              >  1:922480/1‑100 (MQ=255)
                   tGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATa                                                                            >  1:584057/1‑100 (MQ=255)
                    gACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATaa                                                                           <  2:1068779/100‑1 (MQ=255)
                    gACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATaa                                                                           >  2:2549995/1‑100 (MQ=255)
                     aCTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAg                                                                          <  1:2710475/100‑1 (MQ=255)
                      cTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGa                                                                         >  2:1072230/1‑100 (MQ=255)
                      cTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGa                                                                         <  1:686300/100‑1 (MQ=255)
                           tGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCg                                                                    >  2:186918/1‑100 (MQ=255)
                           tGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCg                                                                    <  2:1020235/100‑1 (MQ=255)
                            gTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGc                                                                   >  1:2679846/1‑100 (MQ=255)
                              cAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCa                                                                 <  2:2018626/100‑1 (MQ=255)
                                    tGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGc                                                           <  2:494478/100‑1 (MQ=255)
                                    tGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAAAAAGAATTCGCCATTTGGc                                                           >  2:39144/1‑100 (MQ=255)
                                    tGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTAAAAAAAAAAAAAAATAAGAATTCGCCATTTGGc                                                           <  2:793664/100‑1 (MQ=255)
                                       cTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTTGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGt                                                        >  2:1276802/1‑100 (MQ=255)
                                        tATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTg                                                       >  2:1092935/1‑100 (MQ=255)
                                         aTTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTgg                                                      >  2:2336886/1‑100 (MQ=255)
                                         aTTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTgg                                                      <  1:217088/100‑1 (MQ=255)
                                          tttGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAATGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGc                                                     <  2:1608876/100‑1 (MQ=255)
                                             gCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCAt                                                  <  1:1476375/100‑1 (MQ=255)
                                              cATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCAtt                                                 >  1:2426715/1‑100 (MQ=255)
                                              cATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCAtt                                                 <  1:1024661/100‑1 (MQ=255)
                                               aTAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTc                                                >  1:2487413/1‑100 (MQ=255)
                                               aTAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTc                                                >  1:538368/1‑100 (MQ=255)
                                               aTAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTc                                                >  1:538367/1‑100 (MQ=255)
                                                 aaCTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTa                                              >  1:1932381/1‑100 (MQ=255)
                                                  aCTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTAc                                             >  1:2427882/1‑100 (MQ=255)
                                                   cTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACa                                            <  2:2127035/100‑1 (MQ=255)
                                                    tGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACag                                           >  2:1496769/1‑100 (MQ=255)
                                                     gAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAATTAAGAATTCGCCATTTGGCGGTGGCCATTCTACaga                                          <  1:2032836/100‑1 (MQ=255)
                                                     gAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACaga                                          <  1:956592/100‑1 (MQ=255)
                                                       aTCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAgaga                                        >  2:602093/1‑100 (MQ=255)
                                                         ctcCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATg                                      >  1:2490671/1‑100 (MQ=255)
                                                          tcCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGa                                     >  1:1633256/1‑100 (MQ=255)
                                                           ccACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTGCAGAGATGAc                                    >  2:97849/1‑100 (MQ=255)
                                                           ccACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTGCAGAGATGAc                                    >  2:98264/1‑100 (MQ=255)
                                                            cACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACg                                   >  2:1700420/1‑100 (MQ=255)
                                                            cACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACg                                   >  2:1617000/1‑100 (MQ=255)
                                                             aCCTTAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACgt                                  <  2:2294764/100‑1 (MQ=255)
                                                              ccTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACgtg                                 <  2:751473/100‑1 (MQ=255)
                                                               cTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTGCAGAGATGACgtgt                                <  1:97849/100‑1 (MQ=255)
                                                               cTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTGCAGAGATGACgtgt                                <  1:98264/100‑1 (MQ=255)
                                                               cTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACgtgt                                >  1:1816823/1‑100 (MQ=255)
                                                                tGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTa                               >  1:974534/1‑100 (MQ=255)
                                                                tGAATACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTa                               >  1:1357159/1‑100 (MQ=255)
                                                                  aaTACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGa                             <  1:2744796/100‑1 (MQ=255)
                                                                   aTACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGaa                            <  2:1668986/100‑1 (MQ=255)
                                                                   aTACGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGaa                            >  1:2122369/1‑100 (MQ=255)
                                                                      cGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGaaaa                          >  1:559067/1‑99 (MQ=255)
                                                                      cGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAAt                         <  1:1072232/100‑1 (MQ=255)
                                                                      cGTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAAt                         >  1:2087766/1‑100 (MQ=255)
                                                                       gTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATa                        >  2:2533791/1‑100 (MQ=255)
                                                                       gTTAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATa                        >  2:282620/1‑100 (MQ=255)
                                                                        ttAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAg                       >  1:2182906/1‑100 (MQ=255)
                                                                        ttAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAg                       >  2:1636937/1‑100 (MQ=255)
                                                                         tAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGt                      <  2:1451166/100‑1 (MQ=255)
                                                                         tAAAAAGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGt                      <  2:2783924/100‑1 (MQ=255)
                                                                           aaaaGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTa                    <  1:233858/100‑1 (MQ=255)
                                                                           aaaaGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTa                    >  2:1368041/1‑100 (MQ=255)
                                                                           aaaaGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTa                    >  1:393993/1‑100 (MQ=255)
                                                                            aaaGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTAc                   <  1:940258/100‑1 (MQ=255)
                                                                             aaGACTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTAcc                  <  1:2599852/100‑1 (MQ=255)
                                                                             aaGACTTAAGTAGTGGAAGGCTATAAAAAAAAAAAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTAcc                  <  2:1027561/100‑1 (MQ=255)
                                                                                aCTTAAGTAGTGGAAGGGTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGat               <  2:255548/100‑1 (MQ=255)
                                                                                 cTTAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGata              <  1:2776011/100‑1 (MQ=255)
                                                                                  ttAAGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGatat             >  1:708935/1‑100 (MQ=255)
                                                                                    aaGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGAtata            >  2:721714/1‑99 (MQ=255)
                                                                                    aaGTAGTGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATaa           >  2:2747916/1‑100 (MQ=255)
                                                                                     agtagtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATaaa          >  1:2521284/1‑100 (MQ=255)
                                                                                       ttatagAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATa        <  1:1882471/95‑1 (MQ=255)
                                                                                       tagtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATa        <  2:529582/100‑1 (MQ=255)
                                                                                        agtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAg       <  1:2282706/100‑1 (MQ=255)
                                                                                         gtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGt      >  1:2662265/1‑100 (MQ=255)
                                                                                         gtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGt      >  2:1974339/1‑100 (MQ=255)
                                                                                         gtGGAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGt      >  2:668927/1‑100 (MQ=255)
                                                                                           ggAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGTTa    >  1:2846775/1‑100 (MQ=255)
                                                                                            gAAGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGTTAc   <  1:1636949/100‑1 (MQ=255)
                                                                                             aaGGCTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGTTACa  <  2:2855792/100‑1 (MQ=255)
                                                                                                 |                                                                                               
TCATTCCATGAATAAATATTGACTGCCTGTCAAACATGACTATTTGCATAACTGAATCTCCACCTGAATACGTTAAAAAGACTTAAGTAGTGGAAGGGTATTACCCGCGAGAAAAAATAAGAATTCGCCATTTGGCGGTGGCCATTCTACAGAGATGACGTGTAGAAAATAGTTACCGATATAAATAGTTACA  >  NC_000913/3671846‑3672038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: