Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,811,584 G→A P31S (CCG→TCG)  rpmB ← 50S ribosomal subunit protein L28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,811,5840GA99.1% 381.8 / ‑6.2 113P31S (CCG→TCG) rpmB50S ribosomal subunit protein L28
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (64/48);  total (65/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 113 total aligned reads displayed.

CCTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGGCAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCGGGACATGTCTAT  >  NC_000913/3811487‑3811680
                                                                                                 |                                                                                                
ccTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACa                                                                                                <  2:448332/100‑1 (MQ=255)
ccTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACa                                                                                                <  1:2349961/100‑1 (MQ=255)
ccTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACa                                                                                                <  1:1792730/100‑1 (MQ=255)
ccTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACa                                                                                                <  2:2485611/100‑1 (MQ=255)
 nttttttATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAg                                                                                               >  1:1378979/2‑100 (MQ=255)
 cTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCTCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAg                                                                                               >  2:1136883/1‑100 (MQ=255)
 cTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAg                                                                                               <  1:1195103/100‑1 (MQ=255)
 cTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAg                                                                                               >  2:609411/1‑100 (MQ=255)
 cTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAg                                                                                               >  2:1975673/1‑100 (MQ=255)
   tttttATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGa                                                                                             >  1:1121431/1‑100 (MQ=255)
     tttATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGaaa                                                                                           >  2:428840/1‑100 (MQ=255)
           gATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGt                                                                                     <  1:945592/100‑1 (MQ=255)
            aTTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGtt                                                                                    <  1:1831288/100‑1 (MQ=255)
             ttACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGttt                                                                                   <  2:1446869/100‑1 (MQ=255)
             ttACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGttt                                                                                   <  2:246466/100‑1 (MQ=255)
              tACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTa                                                                                  >  2:112086/1‑100 (MQ=255)
              tACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTa                                                                                  >  1:2544370/1‑100 (MQ=255)
               acacGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAg                                                                                 >  2:1874597/1‑100 (MQ=255)
                cacGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGt                                                                                >  2:660315/1‑100 (MQ=255)
                cacGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCATCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGt                                                                                >  1:2013105/1‑100 (MQ=255)
                 acGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTc                                                                               >  1:1899501/1‑100 (MQ=255)
                 acGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTc                                                                               >  1:2514994/1‑100 (MQ=255)
                  cGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcg                                                                              <  2:2234188/100‑1 (MQ=255)
                  cGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcg                                                                              >  1:2449134/1‑100 (MQ=255)
                    cATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcgcg                                                                            <  2:1129693/100‑1 (MQ=255)
                    cATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcgcg                                                                            <  1:2457888/100‑1 (MQ=255)
                    cATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcgcg                                                                            <  1:1444302/100‑1 (MQ=255)
                    cATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcgcg                                                                            >  1:2294161/1‑100 (MQ=255)
                    cATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTcgcg                                                                            >  1:162569/1‑100 (MQ=255)
                       ctcTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTc                                                                         <  1:2663324/98‑1 (MQ=255)
                       aCCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGGCAGGAAACGGCGTTTAGTCGCGtcc                                                                         >  2:1230180/1‑98 (MQ=255)
                       aCCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTc                                                                         >  2:362754/1‑100 (MQ=255)
                        ccTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCa                                                                        >  2:2597199/1‑100 (MQ=255)
                        ccTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCa                                                                        >  2:975454/1‑100 (MQ=255)
                         cTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAg                                                                       >  2:2663305/1‑100 (MQ=255)
                         cTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAg                                                                       <  2:749706/100‑1 (MQ=255)
                          tttAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGt                                                                      >  1:207043/1‑100 (MQ=255)
                          tttAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGt                                                                      >  2:2073039/1‑100 (MQ=255)
                              gCAGATACGCTCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCCACTGGAAACGGCGTTTAGTCGCGTTCAGTGCGt                                                                  <  2:1899487/100‑1 (MQ=255)
                              gCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGt                                                                  >  1:2046915/1‑100 (MQ=255)
                              gCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGt                                                                  >  1:2624523/1‑100 (MQ=255)
                              gCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGt                                                                  >  1:2239824/1‑100 (MQ=255)
                              gCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGt                                                                  >  1:1042981/1‑100 (MQ=255)
                               cAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTg                                                                 <  1:253590/100‑1 (MQ=255)
                                aGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTgg                                                                <  1:117067/100‑1 (MQ=255)
                                 gATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTggg                                                               <  1:2868074/100‑1 (MQ=255)
                                 gATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTggg                                                               >  1:602532/1‑100 (MQ=255)
                                   tACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGtatctg                                                             >  1:1154580/1‑95 (MQ=255)
                                   tACGCGCAGGGTGACAAAACGCTTCCCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGATTAGTCGCGTTCAGTGCGTGGGaa                                                             >  1:876040/1‑100 (MQ=255)
                                    aCGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAAc                                                            <  1:427660/100‑1 (MQ=255)
                                     cgcgCAGGGTGACAAAACGCTTCTCGCACTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACg                                                           >  1:1336580/1‑100 (MQ=255)
                                       cgcAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgt                                                         >  2:941410/1‑100 (MQ=255)
                                        gcAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgtt                                                        <  1:410726/100‑1 (MQ=255)
                                        gcAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgtt                                                        <  2:2432478/100‑1 (MQ=255)
                                         cAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttg                                                       <  1:878659/100‑1 (MQ=255)
                                         cAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttg                                                       <  1:318816/100‑1 (MQ=255)
                                          aGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttgt                                                      <  1:1769000/100‑1 (MQ=255)
                                           gggTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttgtt                                                     <  1:937978/100‑1 (MQ=255)
                                           gggTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttgtt                                                     >  2:1122841/1‑100 (MQ=255)
                                           gggTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGgttgtt                                                     >  1:2103175/1‑100 (MQ=255)
                                             gTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTAc                                                   <  1:63281/100‑1 (MQ=255)
                                              tGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTAcc                                                  >  1:2607157/1‑100 (MQ=255)
                                               gACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCg                                                 <  2:1121427/100‑1 (MQ=255)
                                               gACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCg                                                 >  2:684728/1‑100 (MQ=255)
                                               gACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCg                                                 >  2:684209/1‑100 (MQ=255)
                                                aCAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCgg                                                <  1:609413/100‑1 (MQ=255)
                                                 cAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGt                                               >  2:1780995/1‑100 (MQ=255)
                                                 cAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGt                                               >  1:1635179/1‑100 (MQ=255)
                                                  aaaaCGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTc                                              >  2:611697/1‑100 (MQ=255)
                                                    aaCGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCAc                                            >  2:2073960/1‑100 (MQ=255)
                                                        cTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGa                                        >  1:1242572/1‑100 (MQ=255)
                                                           ctcGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGc                                     <  2:1797181/100‑1 (MQ=255)
                                                           ctcGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGc                                     >  2:730043/1‑100 (MQ=255)
                                                              gCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTg                                  <  1:1372401/100‑1 (MQ=255)
                                                                tctcAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGcc                                <  1:2334864/100‑1 (MQ=255)
                                                                tctcAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGcc                                <  2:1962405/100‑1 (MQ=255)
                                                                tctcAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGcc                                <  2:1449879/100‑1 (MQ=255)
                                                                   cAACCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAg                              >  2:1213779/1‑99 (MQ=255)
                                                                     aCCCAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTaa                           >  1:1239326/1‑100 (MQ=255)
                                                                      cccAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAAc                          >  1:1550768/1‑100 (MQ=255)
                                                                      cccAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAAc                          <  1:2597218/100‑1 (MQ=255)
                                                                      cccAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAAc                          >  2:1054642/1‑100 (MQ=255)
                                                                       ccAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACt                         >  2:2652292/1‑100 (MQ=255)
                                                                       ccAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACt                         <  2:602530/100‑1 (MQ=255)
                                                                        cAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACtt                        >  2:1217925/1‑100 (MQ=255)
                                                                        cAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACtt                        >  2:2006335/1‑100 (MQ=255)
                                                                        cAGAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACtt                        >  2:1467563/1‑100 (MQ=255)
                                                                          gAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTgg                      <  2:2239811/100‑1 (MQ=255)
                                                                          gAAACGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTgg                      <  1:677495/100‑1 (MQ=255)
                                                                           aaaCGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGc                     <  1:941411/100‑1 (MQ=255)
                                                                              cGGTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGa                  >  1:1421805/1‑100 (MQ=255)
                                                                               ggTGAGAGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGAc                 >  2:938748/1‑100 (MQ=255)
                                                                                  gagaGTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCg              >  2:622296/1‑100 (MQ=255)
                                                                                   agagTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCgg             <  1:1063001/100‑1 (MQ=255)
                                                                                    gagTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCggg            <  2:162568/100‑1 (MQ=255)
                                                                                    gagTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCggg            <  1:2162935/100‑1 (MQ=255)
                                                                                    gagTGCAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCggg            >  2:2549737/1‑100 (MQ=255)
                                                                                         cAGGTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGACAGACTCGGGACATg       >  1:789939/1‑100 (MQ=255)
                                                                                           ggTTCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCGGGACATGTc     <  2:2385651/100‑1 (MQ=255)
                                                                                              tCGACAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCGGGACATGTCTAt  >  1:2558514/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CCTTTTTTATCGATTACACGCATACCTTTAGCAGATACGCGCAGGGTGACAAAACGCTTCTCGCTCTCAACCCAGAAACGGTGAGAGTGCAGGTTCGGCAGGAAACGGCGTTTAGTCGCGTTCAGTGCGTGGGAACGGTTGTTACCGGTCACCGGACGCTTGCCAGTAACTTGGCAGACTCGGGACATGTCTAT  >  NC_000913/3811487‑3811680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑