Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,105,411 A→G V87A (GTT→GCT)  cpxR ← response regulator in two‑component regulatory system with CpxA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,105,4110AG98.1% 362.5 / ‑5.0 104V87A (GTT→GCT) cpxRresponse regulator in two‑component regulatory system with CpxA
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (49/53);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATACCATTTTT  >  NC_000913/4105314‑4105502
                                                                                                 |                                                                                           
gTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGcg                                                                                           <  1:1482336/100‑1 (MQ=255)
gTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGcg                                                                                           <  1:1483647/100‑1 (MQ=255)
gTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGcg                                                                                           >  2:1463615/1‑100 (MQ=255)
  gCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGcgcg                                                                                         >  1:59418/1‑100 (MQ=255)
   cGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGa                                                                                        >  2:306031/1‑100 (MQ=255)
    gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGAt                                                                                       <  2:2830734/100‑1 (MQ=255)
    gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGAt                                                                                       >  1:1286906/1‑100 (MQ=255)
    gAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGAt                                                                                       <  2:788166/100‑1 (MQ=255)
     aaCGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATc                                                                                      >  1:1749279/1‑100 (MQ=255)
      aCGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCa                                                                                     <  2:2554105/100‑1 (MQ=255)
      aCGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCa                                                                                     <  2:2427612/100‑1 (MQ=255)
        ggCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAg                                                                                   <  1:2357098/100‑1 (MQ=255)
        ggCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAg                                                                                   >  1:354792/1‑100 (MQ=255)
         gcgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGt                                                                                  <  1:1744741/100‑1 (MQ=255)
         gcgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGt                                                                                  >  2:264164/1‑100 (MQ=255)
          cgcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGtt                                                                                 >  1:466228/1‑100 (MQ=255)
           gcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTc                                                                                >  2:848983/1‑100 (MQ=255)
           gcAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTc                                                                                <  2:570069/100‑1 (MQ=255)
            cAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCa                                                                               <  1:1569452/100‑1 (MQ=255)
               gATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACAGTATCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGACAACGCGATCAAGTTCACTg                                                                            >  1:2686506/1‑100 (MQ=255)
                  cgcgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcg                                                                         <  2:1006078/100‑1 (MQ=255)
                  cgcgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcg                                                                         <  1:2816923/100‑1 (MQ=255)
                   gcgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgc                                                                        >  1:2786122/1‑100 (MQ=255)
                    cgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcg                                                                       >  2:258709/1‑100 (MQ=255)
                    cgcgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcg                                                                       >  1:1328792/1‑100 (MQ=255)
                      cgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcgcg                                                                     >  1:2087868/1‑100 (MQ=255)
                      cgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcgcg                                                                     <  1:1853068/100‑1 (MQ=255)
                      cgAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcgc                                                                      >  2:486332/1‑99 (MQ=255)
                      cgAATACGTGCCACCAGCTCACGATCATTAAACGCTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCcgcgcg                                                                     <  2:1526415/100‑1 (MQ=255)
                         aTACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCg                                                                  <  2:58265/100‑1 (MQ=255)
                          tACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGt                                                                 >  2:2184713/1‑100 (MQ=255)
                          tACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGt                                                                 <  2:1882752/100‑1 (MQ=255)
                           aCGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTc                                                                >  1:1243514/1‑100 (MQ=255)
                            cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                               >  1:627188/1‑100 (MQ=255)
                            cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                               >  2:1992225/1‑100 (MQ=255)
                            cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                               <  1:1398219/100‑1 (MQ=255)
                            cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                               <  1:1076649/100‑1 (MQ=255)
                            cGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCa                                                               >  1:2127550/1‑100 (MQ=255)
                             gTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCaa                                                              >  2:1807579/1‑100 (MQ=255)
                             gTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCaa                                                              <  1:330913/100‑1 (MQ=255)
                               gCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACa                                                            <  1:2466485/100‑1 (MQ=255)
                               gCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACa                                                            >  2:619576/1‑100 (MQ=255)
                                ccaccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACAt                                                           <  2:224984/100‑1 (MQ=255)
                                 caccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACAt                                                           >  2:1361467/1‑99 (MQ=255)
                                 caccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATa                                                          >  2:749837/1‑100 (MQ=255)
                                 caccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATa                                                          >  2:979898/1‑100 (MQ=255)
                                  accaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATaa                                                         >  1:1609858/1‑100 (MQ=255)
                                   ccaGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAAt                                                        <  1:2831302/100‑1 (MQ=255)
                                    caGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATg                                                       <  2:1361814/100‑1 (MQ=255)
                                    caGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATg                                                       >  2:1224117/1‑100 (MQ=255)
                                      gCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGTCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGAc                                                     <  2:1606326/100‑1 (MQ=255)
                                      gCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGAc                                                     >  1:269366/1‑100 (MQ=255)
                                       cTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACa                                                    >  2:1366167/1‑100 (MQ=255)
                                        tCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAg                                                   <  1:2836190/100‑1 (MQ=255)
                                           cGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCg                                                <  1:2118682/100‑1 (MQ=255)
                                           cGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCg                                                >  2:2234048/1‑100 (MQ=255)
                                           cGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCg                                                >  2:52100/1‑100 (MQ=255)
                                             aTCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTc                                              <  2:2151502/100‑1 (MQ=255)
                                               cATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTg                                            <  1:498474/100‑1 (MQ=255)
                                                aTTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTgg                                           <  2:58032/100‑1 (MQ=255)
                                                 ttAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCAGCGCGCCGTCAACATAATGACAGGCGTCTGgt                                          <  2:1295904/100‑1 (MQ=255)
                                                 tgaaaCGGTTTCGAGAGATAGTCATCTGCGCACAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgt                                          >  1:1946814/3‑100 (MQ=255)
                                                  tAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtg                                         <  2:781505/100‑1 (MQ=255)
                                                  tAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtg                                         <  2:2127537/100‑1 (MQ=255)
                                                  tAAACGGTTTCGGGAGATAGTCATCTGCGCCAAGCTCAAGGCCGGGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgt                                          >  2:1191392/1‑99 (MQ=255)
                                                   aaaCGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtgt                                        >  2:2400356/1‑100 (MQ=255)
                                                     aCGGTTTCGGGAGATAGTCATCTGCGCCCAGCCCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGgtgtgt                                      >  1:1077766/1‑100 (MQ=255)
                                                      cGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTc                                     <  1:2443275/100‑1 (MQ=255)
                                                      cGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTc                                     >  1:1882222/1‑100 (MQ=255)
                                                          ttCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGc                                 <  1:2714164/100‑1 (MQ=255)
                                                            cGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGa                               <  1:1557761/100‑1 (MQ=255)
                                                            cGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGa                               <  2:822465/100‑1 (MQ=255)
                                                             gggAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGaa                              >  1:2560162/1‑100 (MQ=255)
                                                              ggAGATAGTCCTCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                             >  2:1162884/1‑100 (MQ=255)
                                                              ggAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAg                             <  2:1192722/100‑1 (MQ=255)
                                                                agaTAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTg                           <  1:2201794/100‑1 (MQ=255)
                                                                   tAGTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCtt                        <  1:52100/100‑1 (MQ=255)
                                                                     gTCATCTGCGCCCAGCTGAAGGGCGAGAGCGCGCTCAAGTTCACTGCGGAGCGCCGTCAAAATGATGAAAGGCTTAGGGTGGGTCTGGCGAAGGGGttt                       >  2:2809671/1‑99 (MQ=255)
                                                                     gTCATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCtttt                      >  1:1860377/1‑100 (MQ=255)
                                                                       cATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTaa                    <  1:44695/100‑1 (MQ=255)
                                                                       cATCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTaa                    <  2:1328787/100‑1 (MQ=255)
                                                                        aTCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAt                   <  1:1843910/100‑1 (MQ=255)
                                                                        aTCTGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAt                   <  2:466226/100‑1 (MQ=255)
                                                                        aTCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAt                   <  1:2527369/100‑1 (MQ=255)
                                                                           tGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAAtgtg                <  2:659894/100‑1 (MQ=255)
                                                                           tGCGCCCAGCTCAAGGCCGAGAGCGCGATCAAGATCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCAGGTGTGTCTGGCGAAGTGCTTTTAAtgtg                >  2:1737544/1‑100 (MQ=255)
                                                                             cgcCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGTCGAAGTGCTTTTAATGTGTc              <  2:1766642/100‑1 (MQ=255)
                                                                             cgcCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTc              >  2:471502/1‑100 (MQ=255)
                                                                             cgcCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTc              <  1:2098449/100‑1 (MQ=255)
                                                                              gcCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCg             >  1:526706/1‑100 (MQ=255)
                                                                              gcCCAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCg             <  2:962691/100‑1 (MQ=255)
                                                                               cccAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGa            >  2:48571/1‑100 (MQ=255)
                                                                               cccAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGa            <  2:1107438/100‑1 (MQ=255)
                                                                               cccAGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGa            <  1:1830372/100‑1 (MQ=255)
                                                                                  aGCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAc         >  2:233929/1‑100 (MQ=255)
                                                                                   gCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACGGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAcc        <  2:627186/100‑1 (MQ=255)
                                                                                   gCTCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATAcc        >  1:2691640/1‑100 (MQ=255)
                                                                                     tCAAGGCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATACCAt      <  1:645260/100‑1 (MQ=255)
                                                                                         ggCCGAGAGCGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATACCAttttt  >  1:2450948/1‑100 (MQ=255)
                                                                                         ggCCGAGAGCGCGAACAAGTTCACTGCCGCGCGCCGTCAACATCATGACAGGCGTCTGGTGTGGCTTGCGAAGTGCTTTTAATGTGGCGAGAGCAttttt  >  2:2700554/1‑100 (MQ=255)
                                                                                                 |                                                                                           
GTGCGAACGGCGCAGGATCGCGCGAATACGTGCCACCAGCTCACGATCATTAAACGGTTTCGGGAGATAGTCATCTGCGCCCAGCTCAAGGCCGAGAACGCGATCAAGTTCACTGCCGCGCGCCGTCAACATAATGACAGGCGTCTGGTGTGTCTGGCGAAGTGCTTTTAATGTGTCGATACCATTTTT  >  NC_000913/4105314‑4105502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑