Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 933,690 C→T P156L (CCA→CTA)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,6900CT95.3% 335.6 / 2.7 107P156L (CCA→CTA) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base T (55/46);  total (57/50)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 107 total aligned reads displayed.

TACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACCACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGCTGA  >  NC_000913/933595‑933786
                                                                                               |                                                                                                
 aCCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGc                                                                                             <  1:446231/100‑1 (MQ=255)
  cctcctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCt                                                                                            >  2:2529325/1‑100 (MQ=255)
   ctcctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTa                                                                                           >  2:1116682/1‑100 (MQ=255)
   ctcctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTa                                                                                           >  2:451207/1‑100 (MQ=255)
    tcctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTac                                                                                          >  1:1995912/1‑100 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTCCAACTACTGCTac                                                                                          >  1:1546709/1‑99 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTaca                                                                                         >  2:1253075/1‑100 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTaca                                                                                         >  2:201737/1‑100 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTaca                                                                                         >  1:1242330/1‑100 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTaca                                                                                         >  1:592386/1‑100 (MQ=255)
     cctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTaca                                                                                         <  2:2542728/100‑1 (MQ=255)
      ctGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTacac                                                                                        >  1:1250856/1‑100 (MQ=255)
       tgtgGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTacac                                                                                        >  2:1190620/1‑99 (MQ=255)
       tgtgGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTAcaca                                                                                       >  1:1517246/1‑100 (MQ=255)
        gtgGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGATTAATAAGAAATAAGATACAACTACTGCTACACAg                                                                                      <  2:1865880/100‑1 (MQ=255)
         tgGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGt                                                                                     >  1:1823926/1‑100 (MQ=255)
         tgGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACCACTGCTACACAGt                                                                                     <  2:2845158/100‑1 (MQ=255)
           atCTGGCGGCAATCAACGCTGACGATATCGGGGATTGTGCCGCCGTTTGCGGCATGGGCAGCTGACTAAGCACTACGCTACAACGACTGCTACACAGTAg                                                                                   >  2:2411823/2‑100 (MQ=255)
             cTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCg                                                                                 >  1:672256/1‑100 (MQ=255)
               ggcggcAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCggg                                                                               >  1:1232213/1‑100 (MQ=255)
               ggcggcAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCggg                                                                               >  1:50809/1‑100 (MQ=255)
               ggcggcAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCggg                                                                               >  1:48271/1‑100 (MQ=255)
                 cggcAATCAACGCTTACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCggg                                                                               <  2:511156/98‑1 (MQ=255)
                 cggcAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCggggg                                                                             <  1:869188/100‑1 (MQ=255)
                    cAAGCAACGCTGACCATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAAc                                                                          <  2:2697638/100‑1 (MQ=255)
                     aaTCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACt                                                                         >  2:631377/1‑100 (MQ=255)
                       tCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTAt                                                                       >  1:2524213/1‑100 (MQ=255)
                        cAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTAtt                                                                      >  2:280889/1‑100 (MQ=255)
                         aaCGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTg                                                                     <  2:2535945/100‑1 (MQ=255)
                         aaCGCTGACGATATCTCGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTg                                                                     <  2:1695145/100‑1 (MQ=255)
                          aCGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTgc                                                                    <  2:2325718/100‑1 (MQ=255)
                           cGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTgcg                                                                   <  1:1150664/100‑1 (MQ=255)
                            gCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTgcgc                                                                  >  1:2053089/1‑100 (MQ=255)
                              tGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCgct                                                                 >  2:204816/1‑99 (MQ=255)
                               gtcgttatCTGGTATTGTGCCTCCGGTGGCGTCATTGGCAGCTTACGAAGCACTACGCTACAACCACTGCTACATAGTAGCGGGGGAACTATTGCgctgc                                                               <  2:125351/95‑1 (MQ=255)
                                 cGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGctc                                                             >  1:695455/1‑100 (MQ=255)
                                 cGATATCTGGTATTTTGCCTCAGGTGGCGTCATTGGCAGCTTACTAAGCAATACGCTACAACTACTGGTACACAGTAGCGGGGGAACTAtt                                                                      >  2:2168069/1‑91 (MQ=255)
                                   atatCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTg                                                           <  2:897280/100‑1 (MQ=255)
                                   atatCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACCACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTg                                                           <  1:1118158/100‑1 (MQ=255)
                                     atCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCg                                                         <  1:951984/100‑1 (MQ=255)
                                      tCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGt                                                        <  1:2379446/100‑1 (MQ=255)
                                      tCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGt                                                        >  1:1501360/1‑100 (MQ=255)
                                      tCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGt                                                        >  2:1288947/1‑100 (MQ=255)
                                       cTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGtt                                                       >  1:1122685/1‑100 (MQ=255)
                                         ggTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTg                                                     <  1:2829602/100‑1 (MQ=255)
                                         ggTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTg                                                     >  2:1648894/1‑100 (MQ=255)
                                          gTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTgg                                                    <  2:1871672/100‑1 (MQ=255)
                                           tATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTggg                                                   <  1:977715/100‑1 (MQ=255)
                                                  ccTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCggg                                            <  1:2313428/100‑1 (MQ=255)
                                                  ccTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCggg                                            >  1:2064936/1‑100 (MQ=255)
                                                  ccTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCggg                                            >  2:2583240/1‑100 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          >  2:2023137/1‑100 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          <  2:1469533/100‑1 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          <  2:906054/100‑1 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          <  1:2335912/100‑1 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          <  1:2007020/100‑1 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          >  1:776407/1‑100 (MQ=255)
                                                    tCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGcc                                          <  1:81702/100‑1 (MQ=255)
                                                     ccGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCt                                         >  1:1319268/1‑100 (MQ=255)
                                                      cGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTGTTGCGCTGCTCTGCGTTTGGGCAGCGGGCCt                                         <  2:1546700/99‑1 (MQ=255)
                                                      cGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTg                                        <  2:81335/100‑1 (MQ=255)
                                                          ggCGTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACgt                                    >  1:730743/1‑100 (MQ=255)
                                                             gTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACgttgt                                 <  1:1851291/100‑1 (MQ=255)
                                                             gTCATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACgttgt                                 <  2:578228/100‑1 (MQ=255)
                                                               cATTGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTc                               >  1:243191/1‑100 (MQ=255)
                                                               cATTGGCAGCTTACTAAGCACTACGCTACAACCACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTc                               >  2:1570049/1‑100 (MQ=255)
                                                                 ttGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCAc                             >  1:2514069/1‑100 (MQ=255)
                                                                  tGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCAcc                            <  1:1648907/100‑1 (MQ=255)
                                                                  tGGCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCAcc                            <  1:2187884/100‑1 (MQ=255)
                                                                   ggCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCg                           <  2:1061178/100‑1 (MQ=255)
                                                                   ggCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCg                           >  2:215285/1‑100 (MQ=255)
                                                                    gCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCgg                          >  1:2024526/1‑100 (MQ=255)
                                                                    gCAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCgg                          >  1:278889/1‑100 (MQ=255)
                                                                     cAGCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGt                         <  1:1253079/100‑1 (MQ=255)
                                                                       gCTTACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTg                       >  1:15828/1‑100 (MQ=255)
                                                                        cTTACTAAGCACTACGCTACAACCACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTgg                      <  2:302135/100‑1 (MQ=255)
                                                                         ttACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGt                     <  2:1232209/100‑1 (MQ=255)
                                                                          tACTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTc                    <  2:870021/100‑1 (MQ=255)
                                                                           aCTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCa                   >  1:447817/1‑100 (MQ=255)
                                                                            cTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCAt                  >  2:2529553/1‑100 (MQ=255)
                                                                            cTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCAt                  >  2:817207/1‑100 (MQ=255)
                                                                            cTAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCAt                  >  2:2836501/1‑100 (MQ=255)
                                                                             tAAGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATg                 <  2:1250852/100‑1 (MQ=255)
                                                                              aaGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATgg                >  2:1691323/1‑100 (MQ=255)
                                                                               aGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATggg               >  1:1066739/1‑100 (MQ=255)
                                                                               aGCACTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATggg               <  2:755217/100‑1 (MQ=255)
                                                                                 cACTACGCTACAACTACTGCTACACAGTAGCGGAAAAAAAAAAAAAAAGATCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTg             <  2:2053075/100‑1 (MQ=255)
                                                                                   cTACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGAc           >  2:2732631/1‑100 (MQ=255)
                                                                                    tACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGa            <  2:766897/98‑1 (MQ=255)
                                                                                    tACGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGAcc          <  2:1242326/100‑1 (MQ=255)
                                                                                     aCGCTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCa         <  2:695453/100‑1 (MQ=255)
                                                                                        cTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTg      >  2:1824375/1‑100 (MQ=255)
                                                                                        cTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTg      >  2:1726598/1‑100 (MQ=255)
                                                                                        cTACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTg      >  1:255471/1‑100 (MQ=255)
                                                                                         tACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGc     <  2:2064923/100‑1 (MQ=255)
                                                                                         tACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGc     <  2:50809/100‑1 (MQ=255)
                                                                                         tACAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGc     <  2:48271/100‑1 (MQ=255)
                                                                                           cAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGCTg   <  2:592384/100‑1 (MQ=255)
                                                                                           cAACTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGCTg   <  1:1599807/100‑1 (MQ=255)
                                                                                            aaCTACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGCTGa  >  2:1260273/1‑100 (MQ=255)
                                                                                               |                                                                                                
TACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGGCAGCTTACTAAGCACTACGCTACAACCACTGCTACACAGTAGCGGGGGAACTATTGCGCTGCTCTGCGTTTGGGCAGCGGGCCTGACGTTGTTCACCGGTTGGTCATGGGTGACCATTGCTGA  >  NC_000913/933595‑933786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: