Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,168,850 T→C intergenic (‑18/‑223) ycfQ ← / → bhsA repressor for bhsA(ycfR)/biofilm, cell surface and signaling protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,168,8500TC98.1% 371.0 / ‑4.8 108intergenic (‑18/‑223)ycfQ/bhsArepressor for bhsA(ycfR)/biofilm, cell surface and signaling protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (69/36);  total (70/38)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAA  >  NC_000913/1168754‑1168944
                                                                                                |                                                                                              
tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGAc                                                                                             >  1:2158250/1‑100 (MQ=255)
tATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGAc                                                                                             >  1:2889309/1‑100 (MQ=255)
    aaGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACtgtg                                                                                         <  1:199881/100‑1 (MQ=255)
     aGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGtga                                                                                        >  2:1888262/1‑100 (MQ=255)
     aGCGCGGCATCCCTGTCGAACACTTTGGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACtgtg                                                                                         <  1:15693/99‑1 (MQ=255)
      gcgcGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGtgaa                                                                                       >  1:483740/1‑100 (MQ=255)
       cgcgGCATCCTTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGtgaat                                                                                      <  1:523952/100‑1 (MQ=255)
        gcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGtgaatg                                                                                     >  1:2729706/1‑100 (MQ=255)
        gcgGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGtgaatg                                                                                     <  2:2331338/100‑1 (MQ=255)
           gCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGaaa                                                                                  >  2:1602908/1‑100 (MQ=255)
            cATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAg                                                                                 <  1:2468941/100‑1 (MQ=255)
               cgcTGTCGAACACATTTGGGCGGCCACGGCTATTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGtt                                                                              >  1:1949666/3‑100 (MQ=255)
               cccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGtt                                                                              >  1:1367147/1‑100 (MQ=255)
               cccTGTCGAACACTTTTGGGCGGCCACGGCGTTTTTTTACACATTGTGTGTAGTCAGTGGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGtt                                                                              >  1:1580592/1‑100 (MQ=255)
                ccTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGtta                                                                             >  1:578351/1‑100 (MQ=255)
                 cTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGttat                                                                            <  1:292854/100‑1 (MQ=255)
                  tGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTATCTGGCGACTGTGCATGAAAGGgtatt                                                                           >  1:1725269/1‑100 (MQ=255)
                   gTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGttatta                                                                          <  1:2460106/100‑1 (MQ=255)
                     cGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATtata                                                                        <  2:1746595/100‑1 (MQ=255)
                      gAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATaa                                                                       >  1:1991388/1‑100 (MQ=255)
                       aaCACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATaaa                                                                      <  2:446347/100‑1 (MQ=255)
                        acacTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATaaaa                                                                     <  1:970941/100‑1 (MQ=255)
                         cacTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATaaaaa                                                                    >  1:1043250/1‑100 (MQ=255)
                           cTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAaata                                                                  >  1:1912057/1‑100 (MQ=255)
                           cTTTTGGGCGGCCACGGCTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAaataa                                                                 >  1:2464420/1‑100 (MQ=255)
                              ttGGGAGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGACATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTAAAAAAAAAATAATc                                                               <  2:192429/100‑1 (MQ=255)
                               tGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCa                                                              <  2:2763229/100‑1 (MQ=255)
                               tGGGCGGCCACGGCTATTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCa                                                              <  1:2849164/100‑1 (MQ=255)
                               cgatctgcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCa                                                              <  2:74102/94‑1 (MQ=255)
                                 ggcggcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTTGAGACTGTGAATGAAACGTTATTATACAaataattct                                                             >  2:2825903/1‑96 (MQ=255)
                                     gcCACGGCTTTTTTTTATACATTGTTTTTATTCAGTTGCCATGATTCCGTTTTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTcc                                                         >  2:2848877/1‑99 (MQ=255)
                                     gcCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCaagatcg                                                        >  1:74102/1‑94 (MQ=255)
                                        aCGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCAGTTGTACCTGGCGACTGTGAATGAAAAAAAAAAAAAAAAATAATCAcc                                                            <  2:1656501/93‑1 (MQ=255)
                                         cGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCa                                                    >  1:1130773/1‑100 (MQ=255)
                                          ggCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCAc                                                   <  2:2153673/100‑1 (MQ=255)
                                          ggCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCAc                                                   >  1:841250/1‑100 (MQ=255)
                                           gCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCAcc                                                  <  2:1498982/100‑1 (MQ=255)
                                           gCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCAcc                                                  <  2:2729685/100‑1 (MQ=255)
                                              tttttttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGNTATNATAAAAATAATCACCTCCGTTCACCAGt                                               >  1:479/1‑100 (MQ=255)
                                                 ttttGCACATGGTGTTGAGTCAGTTGCCATGAGGCCTGTGGACCTGGCTACTGTGAATGAAAGGTTATTATAAAAATAATCGCCTCCGTTCACCAGTCCa                                            <  2:1031754/100‑1 (MQ=255)
                                                  tttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAg                                           >  2:1869472/1‑100 (MQ=255)
                                                  tttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAg                                           >  1:1845547/1‑100 (MQ=255)
                                                  tttACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAg                                           >  1:329063/1‑100 (MQ=255)
                                                    tACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGAt                                         >  2:253083/1‑100 (MQ=255)
                                                      cacaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATcc                                       >  1:2138669/1‑100 (MQ=255)
                                                      cacaTTGTGTGGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATcc                                       <  1:270615/100‑1 (MQ=255)
                                                       acaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATccc                                      <  2:1292767/100‑1 (MQ=255)
                                                       acaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATccc                                      <  1:1465142/100‑1 (MQ=255)
                                                       acaTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATccc                                      >  1:2573877/1‑100 (MQ=255)
                                                        caTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCa                                     >  1:2525625/1‑100 (MQ=255)
                                                         aTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCa                                     <  2:1993511/99‑1 (MQ=255)
                                                         aTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCAt                                    >  1:1326062/1‑100 (MQ=255)
                                                         aTTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCAt                                    >  2:411856/1‑100 (MQ=255)
                                                          ttGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATa                                   >  1:122649/1‑100 (MQ=255)
                                                            gtgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaa                                 <  1:411857/100‑1 (MQ=255)
                                                            gtgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaa                                 >  1:2383604/1‑100 (MQ=255)
                                                             tgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaaa                                >  1:678155/1‑100 (MQ=255)
                                                             tgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaaa                                >  1:146668/1‑100 (MQ=255)
                                                             tgtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaaa                                >  1:236075/1‑100 (MQ=255)
                                                              gtTGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATaaaaa                               >  1:2646594/1‑100 (MQ=255)
                                                               ttGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaat                              >  1:1704492/1‑100 (MQ=255)
                                                               ttGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaat                              <  2:2174999/100‑1 (MQ=255)
                                                                tGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaata                             >  2:2116956/1‑100 (MQ=255)
                                                                tGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaata                             >  1:2175012/1‑100 (MQ=255)
                                                                tGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaata                             >  2:2199184/1‑100 (MQ=255)
                                                                tGAGTCAGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAaata                             >  1:2270011/1‑100 (MQ=255)
                                                                      aGTTGCCATGATGGCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCt                       >  1:1958038/1‑100 (MQ=255)
                                                                      aGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCt                       >  1:1836696/1‑100 (MQ=255)
                                                                      aGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCt                       >  1:2279579/1‑100 (MQ=255)
                                                                      aGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCt                       >  2:1466066/1‑100 (MQ=255)
                                                                      aGTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCt                       <  1:1801519/100‑1 (MQ=255)
                                                                       gTTGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCtt                      >  1:1596346/1‑100 (MQ=255)
                                                                         tGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTc                    >  1:1769169/1‑100 (MQ=255)
                                                                         tGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTc                    <  2:42438/100‑1 (MQ=255)
                                                                         tGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTc                    <  2:578349/100‑1 (MQ=255)
                                                                         tGCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTc                    >  2:2336418/1‑100 (MQ=255)
                                                                         tGCCATGATGCCGTTGTACCTGGCGACTGTAAAAGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAAAAATTGCTTTc                    >  2:1896913/1‑100 (MQ=255)
                                                                          nccATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCt                   >  2:2568959/2‑100 (MQ=255)
                                                                          gCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCt                   >  1:1070396/1‑100 (MQ=255)
                                                                          gCCATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCt                   >  2:126525/1‑100 (MQ=255)
                                                                          ccgatcttgCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCt                   <  2:1056511/93‑1 (MQ=255)
                                                                           ccATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTa                  >  2:2310484/1‑100 (MQ=255)
                                                                            cATGATGCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTAt                 <  1:2438600/100‑1 (MQ=255)
                                                                            cATGATGCCGTTGTACCTGGCGACTGTGAATCACAGGTTATTATAAAAATAATAACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTac                 <  2:1580583/100‑2 (MQ=255)
                                                                             atgatgCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTAtt                >  2:1998136/1‑100 (MQ=255)
                                                                                 ttcctttGTACCTGGTGACCTTCAATGAATGGTTCTTATAACAGTAATCACCTCCGTTCATCAGTCCAGATCCCATAATAATAATTTCTTTCTAttga              <  2:719998/93‑3 (MQ=255)
                                                                                 tgCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTagatcgg            >  1:1056513/1‑94 (MQ=255)
                                                                                 tgCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAAc            >  2:628464/1‑100 (MQ=255)
                                                                                  gCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACt           <  2:1479312/100‑1 (MQ=255)
                                                                                  gCCGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACt           >  1:106119/1‑100 (MQ=255)
                                                                                    cGTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGa         >  2:1109941/1‑100 (MQ=255)
                                                                                     gTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACGAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGaa        >  2:2023162/1‑100 (MQ=255)
                                                                                     gTTGTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCAAAAAAAAAAAAAAATAATTGCTTTCTATTTAACt           <  2:1324663/97‑1 (MQ=255)
                                                                                        gTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAtt     >  2:2148238/1‑100 (MQ=255)
                                                                                        gTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAtt     >  2:1240571/1‑100 (MQ=255)
                                                                                        gTACCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAAtt     >  2:1240559/1‑100 (MQ=255)
                                                                                          aCCTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTa   >  2:1170918/1‑100 (MQ=255)
                                                                                           ccTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTaa  >  1:1775752/1‑100 (MQ=255)
                                                                                           ccTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTaa  >  2:1542559/1‑100 (MQ=255)
                                                                                           ccTGGCGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTaa  <  2:483738/100‑1 (MQ=255)
                                                                                                |                                                                                              
TATCAAGCGCGGCATCCCTGTCGAACACTTTTGGGCGGCCACGGCTTTTTTTTACACATTGTGTTGAGTCAGTTGCCATGATGCCGTTGTACCTGGTGACTGTGAATGAAAGGTTATTATAAAAATAATCACCTCCGTTCACCAGTCCAGATCCCATAAAAATAATTGCTTTCTATTTAACTGAAATTTAA  >  NC_000913/1168754‑1168944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: