Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,610,183 G→A I166I (ATC→ATT uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,610,1830GA99.1% 363.2 / ‑4.6 106I166I (ATC→ATTuxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (62/42);  total (64/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 109 total aligned reads displayed.

AATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCG  >  NC_000913/1610087‑1610279
                                                                                                |                                                                                                
aaTAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATc                                                                                               >  2:1758625/1‑100 (MQ=255)
   aaaTGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCaa                                                                                            >  1:187496/1‑100 (MQ=255)
    aaTGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAAc                                                                                           >  2:276355/1‑100 (MQ=255)
    aaTGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAAc                                                                                           <  2:1446208/100‑1 (MQ=255)
     aTGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAAcc                                                                                          <  2:2219655/100‑1 (MQ=255)
        cTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCttt                                                                                       >  2:2478111/1‑100 (MQ=255)
           cTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATc                                                                                    >  1:2461763/1‑100 (MQ=255)
           cTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATc                                                                                    >  2:2074749/1‑100 (MQ=255)
            tGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATcc                                                                                   <  2:2223193/100‑1 (MQ=255)
            tGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATcc                                                                                   >  2:515307/1‑100 (MQ=255)
             ggCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCa                                                                                  >  1:386249/1‑100 (MQ=255)
             ggCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCa                                                                                  >  1:1593017/1‑100 (MQ=255)
             ggCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCa                                                                                  >  1:945695/1‑100 (MQ=255)
               cAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAgc                                                                                <  1:76216/100‑1 (MQ=255)
                aGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAgcg                                                                               >  2:1966022/1‑100 (MQ=255)
                aGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAgcg                                                                               >  1:1946586/1‑100 (MQ=255)
                 gTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAgcgc                                                                              <  2:2132984/100‑1 (MQ=255)
                   gCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCAc                                                                            >  1:658398/1‑100 (MQ=255)
                   gCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCAc                                                                            >  1:1415304/1‑100 (MQ=255)
                     ccACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCg                                                                          >  1:669493/1‑100 (MQ=255)
                     ccACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCg                                                                          >  1:1535382/1‑100 (MQ=255)
                      cACTCTTGTGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGt                                                                         >  2:2881154/1‑100 (MQ=255)
                      cACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGt                                                                         <  2:721318/100‑1 (MQ=255)
                        ctctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTg                                                                       >  2:2383293/1‑100 (MQ=255)
                        ctctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTg                                                                       >  1:245142/1‑100 (MQ=255)
                          ctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGaa                                                                     >  1:2185959/1‑100 (MQ=255)
                          ctTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGaa                                                                     >  1:2142154/1‑100 (MQ=255)
                               gcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                >  2:2664091/1‑100 (MQ=255)
                               gcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                <  1:1900379/100‑1 (MQ=255)
                               gcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                >  1:2860548/1‑100 (MQ=255)
                               gcATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                <  2:1411103/100‑1 (MQ=255)
                                cATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGc                                                                <  1:1900418/99‑1 (MQ=255)
                                cATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCt                                                               >  2:2689440/1‑100 (MQ=255)
                                  tAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTaa                                                             >  1:2852038/1‑100 (MQ=255)
                                  tAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTaa                                                             >  2:1485163/1‑100 (MQ=255)
                                    gcgcAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAg                                                           >  2:1937968/1‑100 (MQ=255)
                                    gcgcAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAg                                                           <  1:607988/100‑1 (MQ=255)
                                     cgcAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGc                                                          <  2:1473799/100‑1 (MQ=255)
                                      ncagcaCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATACAGCGCACCGTTGAAATGGCTAAAGCg                                                         >  2:1302546/2‑100 (MQ=255)
                                          caCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCg                                                     <  1:2318248/100‑1 (MQ=255)
                                          caCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCg                                                     <  2:166141/100‑1 (MQ=255)
                                          caCCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCg                                                     <  2:1955825/100‑1 (MQ=255)
                                             cAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAc                                                  <  2:452192/100‑1 (MQ=255)
                                              aGTTCACGCGACGCGTCGCCATTGTAGTCAATCAACTCACTCGGAATGATAATCCAACCTTTATCCAGCCCACCGTTGAAATGGCTAAAGCGTTc                                                      >  2:644527/1‑95 (MQ=255)
                                               gTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcag                                                >  1:2253771/1‑100 (MQ=255)
                                                 tCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcagca                                              >  1:2176510/1‑100 (MQ=255)
                                                 tCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcagca                                              <  2:1399655/100‑1 (MQ=255)
                                                 tCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAAcagca                                              <  1:1466270/100‑1 (MQ=255)
                                                    cGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGAc                                           <  1:787489/100‑1 (MQ=255)
                                                    cGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGAGAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGAc                                           <  2:1853745/100‑1 (MQ=255)
                                                         cgcgTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTc                                      <  1:2636149/100‑1 (MQ=255)
                                                         cgcgTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTc                                      >  1:1604883/1‑100 (MQ=255)
                                                           cgTCGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAg                                    >  2:645123/1‑100 (MQ=255)
                                                              cGCCATTGTAGTCAATCAACTCACACGGCATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGCTCGAACAGCAGACGGGTCAGttt                                 >  2:1964430/1‑100 (MQ=255)
                                                              cGCCATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGttt                                 <  2:386248/100‑1 (MQ=255)
                                                                ccATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTg                               >  1:2844194/1‑100 (MQ=255)
                                                                ccATTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTg                               <  2:2120926/100‑1 (MQ=255)
                                                                  aTTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGcc                             <  1:2730206/100‑1 (MQ=255)
                                                                  aTTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGcc                             <  1:2478127/100‑1 (MQ=255)
                                                                  aTTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGcc                             <  2:187495/100‑1 (MQ=255)
                                                                  aTTGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGcc                             <  1:263221/100‑1 (MQ=255)
                                                                   ttGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCg                            <  2:651661/100‑1 (MQ=255)
                                                                   ttGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCg                            <  2:424634/100‑1 (MQ=255)
                                                                    tGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCgg                           >  1:711877/1‑100 (MQ=255)
                                                                    tGTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAACAGACGGGTCAGTTTTGCCgg                           <  1:2660535/100‑1 (MQ=255)
                                                                     gTAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGa                          >  1:2026368/1‑100 (MQ=255)
                                                                      tAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGAt                         >  2:740553/1‑100 (MQ=255)
                                                                       aGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATa                        >  1:2090028/1‑100 (MQ=255)
                                                                       aGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATa                        >  2:1236087/1‑100 (MQ=255)
                                                                       aGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATa                        <  1:2881175/100‑1 (MQ=255)
                                                                       aGTCAATCAACTCACACGGAATGATATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAg                       >  2:1627087/1‑100 (MQ=255)
                                                                         tcaatcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGc                      >  1:2329487/1‑100 (MQ=255)
                                                                          caatcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCt                     >  2:641554/1‑100 (MQ=255)
                                                                          caatcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCt                     >  2:830038/1‑100 (MQ=255)
                                                                          caatcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCt                     >  2:2301491/1‑100 (MQ=255)
                                                                          caatcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCt                     >  2:1944028/1‑100 (MQ=255)
                                                                            atcaaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGCCGGGTCAGTTTTGCCGGATAGCTTa                   >  2:2354486/1‑100 (MQ=255)
                                                                               aaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCg                >  1:1436720/1‑100 (MQ=255)
                                                                               aaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCg                <  1:407244/100‑1 (MQ=255)
                                                                               aaCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCg                >  2:526024/1‑100 (MQ=255)
                                                                                aCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGc               >  2:2678334/1‑100 (MQ=255)
                                                                                aCTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGc               >  2:109143/1‑100 (MQ=255)
                                                                                 cTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCt              >  1:2549931/1‑100 (MQ=255)
                                                                                 cTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCt              <  2:245141/100‑1 (MQ=255)
                                                                                   cacaCGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTgg            >  1:1991444/1‑100 (MQ=255)
                                                                                   cacaCGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTgg            >  2:674307/1‑100 (MQ=255)
                                                                                   cacaCGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTgg            <  2:2363057/100‑1 (MQ=255)
                                                                                   cacaCGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTgg            >  1:671568/1‑100 (MQ=255)
                                                                                    acacGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGgc           <  2:1946572/100‑1 (MQ=255)
                                                                                    acacGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGgc           >  1:2612084/1‑100 (MQ=255)
                                                                                     cacGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGgcg          <  2:669491/100‑1 (MQ=255)
                                                                                       cGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTATAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGcgcg        >  2:1609398/1‑100 (MQ=255)
                                                                                       cGGAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGcgcg        <  2:671566/100‑1 (MQ=255)
                                                                                         gAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTc      >  1:817909/1‑100 (MQ=255)
                                                                                         gAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTc      >  1:803262/1‑100 (MQ=255)
                                                                                         gAATGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTc      <  2:2090015/100‑1 (MQ=255)
                                                                                          aaTGATAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCa     <  2:2110565/100‑1 (MQ=255)
                                                                                           atgataATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCAt    >  2:557395/1‑100 (MQ=255)
                                                                                             gataatCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCg  >  2:2424650/1‑100 (MQ=255)
                                                                                             gataatCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGACAGTTTTGCCGGATACCTTACCGCTGGCGCGTCATCg  >  2:306468/1‑100 (MQ=255)
                                                                                                |                                                                                                
AATAAATGCTTCTGGCAGTGCCCACTCTTGCGCATAGCGCAGCACCAGTTCACGCAACGCGTCGCCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCG  >  NC_000913/1610087‑1610279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: