Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,399,911 T→C K85K (AAA→AAG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,9110TC95.2% 330.7 / 2.1 104K85K (AAA→AAG) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (57/41);  total (60/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGT  >  NC_000913/2399815‑2400002
                                                                                                |                                                                                           
cATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTc                                                                                          >  2:2136366/1‑100 (MQ=255)
cATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTc                                                                                          <  1:1894898/100‑1 (MQ=255)
 atatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCg                                                                                         >  2:2876195/1‑100 (MQ=255)
   atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGct                                                                                       >  1:1075615/1‑100 (MQ=255)
   atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGct                                                                                       <  1:1689938/100‑1 (MQ=255)
     tgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGCGGTTGGTCATCAACCCCTCGACCACGCTTTCACGGAACTgcttcgcttc                                                                                     >  1:2170280/3‑100 (MQ=25)
     ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTgcttcgcttc                                                                                     >  1:47394/1‑100 (MQ=255)
     ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTgcttcgcttc                                                                                     >  2:2620896/1‑100 (MQ=255)
     ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACAGAACTgcttcgcttc                                                                                     <  2:2402099/100‑1 (MQ=255)
      gtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttct                                                                                    >  2:241849/2‑100 (MQ=37)
      gtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttct                                                                                    >  2:2822662/2‑100 (MQ=37)
      gtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttct                                                                                    >  2:761311/2‑100 (MQ=37)
      gtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttct                                                                                    >  2:2319943/2‑100 (MQ=37)
      cTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttct                                                                                    >  2:1456257/1‑100 (MQ=255)
       tGAAGATGGCCGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttctt                                                                                   <  1:1396086/100‑1 (MQ=255)
       tGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttctt                                                                                   <  1:251606/100‑1 (MQ=255)
       tGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGcttctt                                                                                   >  1:449015/1‑100 (MQ=37)
           gATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTtcgtc                                                                               <  2:2208345/100‑1 (MQ=255)
            aTGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTtcgtcg                                                                              >  1:1053409/1‑100 (MQ=255)
             tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTcgtcgt                                                                             <  1:1473729/100‑1 (MQ=255)
             tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTcgtcgt                                                                             >  2:2006885/1‑100 (MQ=255)
             tGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTcgtcgt                                                                             >  1:2721965/1‑100 (MQ=255)
               gCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCa                                                                           >  1:329047/1‑100 (MQ=255)
                cAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCaa                                                                          >  1:682692/1‑100 (MQ=255)
                   ttACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATgg                                                                       <  2:1162635/100‑1 (MQ=255)
                    tACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGa                                                                      >  2:212782/1‑100 (MQ=255)
                    tACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGa                                                                      >  2:213155/1‑100 (MQ=255)
                      ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaaa                                                                    >  1:2798953/1‑100 (MQ=255)
                      ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGaaa                                                                    <  1:683322/100‑1 (MQ=255)
                      ccGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGaaa                                                                    <  2:2481490/100‑1 (MQ=255)
                        gCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATa                                                                  <  2:1163394/100‑1 (MQ=255)
                         cccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATaa                                                                 <  1:2372349/100‑1 (MQ=255)
                            tctTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                              <  1:2737508/100‑1 (MQ=255)
                            tctTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAgg                                                              <  2:182335/100‑1 (MQ=255)
                               tCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGt                                                             >  2:688937/1‑98 (MQ=255)
                               tCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGc                                                           >  1:495046/1‑100 (MQ=255)
                                cacaTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGcc                                                          >  2:897697/1‑100 (MQ=255)
                                cacaTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGcc                                                          >  2:897923/1‑100 (MQ=255)
                                  caTACCGGACGGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCAt                                                        >  2:2034289/1‑100 (MQ=255)
                                  caTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCAt                                                        >  2:413305/1‑100 (MQ=255)
                                   aTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATc                                                       <  2:1108579/100‑1 (MQ=255)
                                    tACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                      <  2:1567102/100‑1 (MQ=255)
                                    tACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                                      >  1:1680664/1‑100 (MQ=255)
                                     aCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                                                     <  1:655687/100‑1 (MQ=255)
                                     aCCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCgg                                                     <  1:2777239/100‑1 (MQ=255)
                                      ccGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGa                                                    >  2:192258/1‑100 (MQ=255)
                                       cGGACAGTCGTGCGGGTGGTTCGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGaa                                                   <  2:1426468/100‑1 (MQ=255)
                                          aCAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGcc                                                <  1:951657/100‑1 (MQ=255)
                                           cAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCg                                               >  2:2454212/1‑100 (MQ=255)
                                             ntCGTGCGGGTGGTTGGTCCTCAACCACTTGACCACGCTTTCACGGTACTCCTTCGCCTCTTCGTCGTCGATGCAAATGAACGTTCCCTCGGAACCCgg                                              >  2:2866450/2‑99 (MQ=255)
                                             gTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                                             >  1:2330875/1‑100 (MQ=255)
                                              tCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                            <  2:329046/100‑1 (MQ=255)
                                              tCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                            <  2:482216/100‑1 (MQ=255)
                                              tCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                                            <  1:2002906/100‑1 (MQ=255)
                                               tggtcgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtgt                                           >  2:2146988/8‑100 (MQ=38)
                                               cGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtgt                                           >  1:2314210/1‑100 (MQ=255)
                                                gTGCGTGTTGTTGGTCATCAATCTCTCGACCCCCCGTTCACGGAACTGCTTCGCTTCTTCCTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                                             <  2:2430520/97‑1 (MQ=255)
                                                gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTc                                          <  2:1007596/100‑1 (MQ=255)
                                                gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTc                                          <  1:876539/100‑1 (MQ=255)
                                                gTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTc                                          <  1:193490/100‑1 (MQ=255)
                                                 tGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCa                                         <  2:1445170/100‑1 (MQ=255)
                                                 tGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCa                                         <  1:343432/100‑1 (MQ=255)
                                                  gCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                                        <  1:1015813/100‑1 (MQ=255)
                                                   cgagtggtTGATGACCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                                       >  2:2282704/4‑100 (MQ=255)
                                                   cGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                                       >  2:2111876/1‑100 (MQ=255)
                                                     ggtggtTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACa                                     >  2:1021540/1‑100 (MQ=255)
                                                       tggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                                   <  1:2876216/100‑1 (MQ=255)
                                                        ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa                                  <  2:1627415/100‑1 (MQ=255)
                                                        ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa                                  <  2:1628885/100‑1 (MQ=255)
                                                        ggttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGa                                  >  1:2302931/1‑100 (MQ=255)
                                                         gttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                 >  2:949003/1‑100 (MQ=255)
                                                         gttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                                 <  1:380354/100‑1 (MQ=255)
                                                          ttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                <  1:2454228/100‑1 (MQ=255)
                                                          ttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                >  1:1462573/1‑100 (MQ=255)
                                                          ttggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACa                                >  2:649240/1‑100 (MQ=255)
                                                           tgatgaccaACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAACTAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACAt                               >  2:604166/8‑100 (MQ=255)
                                                            ggtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATc                              >  1:83557/1‑100 (MQ=255)
                                                             gtCATCAACCACTCGACCACGCTTTCACGGCACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCa                             >  1:1004240/1‑100 (MQ=255)
                                                             gtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCa                             >  1:2484092/1‑100 (MQ=255)
                                                             gtCATCAACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCa                             >  2:294504/1‑100 (MQ=255)
                                                              tcatcaACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAc                            <  2:469042/100‑1 (MQ=255)
                                                               catcaACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc                           >  2:387891/1‑100 (MQ=255)
                                                                atcaACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCa                          >  1:1977710/1‑100 (MQ=255)
                                                                 tcaACCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAg                         >  1:2884159/1‑100 (MQ=255)
                                                                   aaCCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAgg                        >  2:436553/1‑99 (MQ=255)
                                                                    aCCACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGATGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCg                      >  1:2342235/1‑100 (MQ=255)
                                                                      cACTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAc                    <  1:213156/100‑1 (MQ=255)
                                                                       aCTCGACCACGCTTTCACGGAACTGGTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAcc                   <  1:2752862/100‑1 (MQ=255)
                                                                       aCTCGACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGAcc                   >  1:743995/1‑100 (MQ=255)
                                                                           gACCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcgc               >  2:1096463/1‑100 (MQ=255)
                                                                            aCCACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATATAGGTCATCACCAGGGGACCAcgcg              <  2:1730554/100‑1 (MQ=255)
                                                                              caggCTTTCACGCAACTGTTTCGCTTCATAGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg            <  2:1496460/97‑1 (MQ=255)
                                                                              cACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg            <  1:2136379/100‑1 (MQ=255)
                                                                              cACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg            <  1:2822683/100‑1 (MQ=255)
                                                                              cACGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCgtg            <  2:2580788/100‑1 (MQ=255)
                                                                                cGCTTTCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTc          >  1:563024/1‑100 (MQ=255)
                                                                                    ttCACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTcc      >  1:250134/1‑100 (MQ=255)
                                                                                      cACGGAACTGCTTCGCTTCTTCGTCGTCAATGGCAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCcgc    >  1:872587/1‑100 (MQ=255)
                                                                                      cACGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCcgc    >  2:2869/1‑100 (MQ=255)
                                                                                        cGGAACTGCTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGt  >  2:2882085/1‑100 (MQ=255)
                                                                                                |                                                                                           
CATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGCGTGTCTTCCGCGT  >  NC_000913/2399815‑2400002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑