Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,553,210 A→G K287E (AAA→GAA)  amiA → N‑acetylmuramoyl‑l‑alanine amidase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,553,2100AG98.9% 317.2 / ‑4.6 90K287E (AAA→GAA) amiAN‑acetylmuramoyl‑l‑alanine amidase I
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (47/42);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

TTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCG  >  NC_000913/2553114‑2553303
                                                                                                |                                                                                             
ttAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGTAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGgaaa                                                                                            >  2:2119537/1‑100 (MQ=255)
 tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGgaaaa                                                                                           <  2:34421/100‑1 (MQ=255)
 tAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGgaaa                                                                                            >  1:2771453/1‑99 (MQ=255)
  aGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAg                                                                                          >  2:475707/1‑100 (MQ=255)
  aGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAg                                                                                          <  2:1422644/100‑1 (MQ=255)
   ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGc                                                                                         >  1:311599/1‑100 (MQ=255)
   ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGc                                                                                         <  1:2023591/100‑1 (MQ=255)
   ggCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTACGACAACCAGAAAGCACATTCGGAAAAGc                                                                                         >  2:1734522/1‑100 (MQ=255)
     cACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGa                                                                                       <  1:555868/100‑1 (MQ=255)
      aCGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGAt                                                                                      >  2:2590358/1‑100 (MQ=255)
       cggcggCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATa                                                                                     >  1:640123/1‑100 (MQ=255)
       cggcggCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATa                                                                                     <  1:1083601/100‑1 (MQ=255)
         gcggcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAg                                                                                   <  1:1870566/100‑1 (MQ=255)
            gcgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTa                                                                                <  2:1643436/100‑1 (MQ=255)
             cgTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTAt                                                                               <  2:2583824/100‑1 (MQ=255)
                ttCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGaa                                                                            <  2:7609/100‑1 (MQ=255)
                ttCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGaa                                                                            <  2:36914/100‑1 (MQ=255)
                    tCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAAccc                                                                        <  2:393713/100‑1 (MQ=255)
                     cAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCg                                                                       >  1:2044434/1‑100 (MQ=255)
                     cAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCg                                                                       >  2:527755/1‑100 (MQ=255)
                      aGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGa                                                                      >  2:2044958/1‑100 (MQ=255)
                      aGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGa                                                                      <  1:2785302/100‑1 (MQ=255)
                       gAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGAc                                                                     <  1:234688/100‑1 (MQ=255)
                        aaaaTCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACg                                                                    >  2:2444288/1‑100 (MQ=255)
                          aaTCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGca                                                                  <  2:1018324/100‑1 (MQ=255)
                          aaTCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGca                                                                  <  1:722490/100‑1 (MQ=255)
                                cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg                                                            <  1:2324687/100‑1 (MQ=255)
                                cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg                                                            >  1:2271262/1‑100 (MQ=255)
                                cacaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg                                                            >  1:2501837/1‑100 (MQ=255)
                                 acaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAgg                                                           >  1:2492470/1‑100 (MQ=255)
                                 acaGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAg                                                            <  1:2323019/99‑1 (MQ=255)
                                   aGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGtt                                                         >  2:2594460/1‑100 (MQ=255)
                                   aGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGtt                                                         >  1:1629038/1‑100 (MQ=255)
                                    gCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGATGCACACCAGGTTa                                                        <  2:1789814/100‑1 (MQ=255)
                                    gCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTa                                                        >  1:2203426/1‑100 (MQ=255)
                                      gATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTaan                                                      <  1:2257875/100‑2 (MQ=255)
                                       aTTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAAc                                                     <  2:2881926/100‑1 (MQ=255)
                                       aTTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAAc                                                     <  2:810580/100‑1 (MQ=255)
                                        ttGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACa                                                    <  1:113266/100‑1 (MQ=255)
                                         tGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAg                                                   >  2:1630212/1‑100 (MQ=255)
                                          gCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGt                                                  >  1:517826/1‑100 (MQ=255)
                                          gCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGt                                                  >  1:2279136/1‑100 (MQ=255)
                                           cTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGtt                                                 >  2:585/1‑100 (MQ=255)
                                           cTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGtt                                                 >  2:1396840/1‑100 (MQ=255)
                                            tGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGttt                                                >  1:1314244/1‑100 (MQ=255)
                                             gAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTc                                               >  1:2329511/1‑100 (MQ=255)
                                              aaGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCt                                              <  2:2492454/100‑1 (MQ=255)
                                                ggCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGc                                            <  1:1616904/100‑1 (MQ=255)
                                                 gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATCAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt                                           >  2:2031925/1‑100 (MQ=255)
                                                 gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt                                           <  1:2119550/100‑1 (MQ=255)
                                                 gCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCt                                           >  2:2092289/1‑100 (MQ=255)
                                                    tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                        >  1:1253402/1‑100 (MQ=255)
                                                    tGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCaa                                        <  2:311598/100‑1 (MQ=255)
                                                     gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAc                                       >  2:100026/1‑100 (MQ=255)
                                                     gATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAAc                                       >  1:9186/1‑100 (MQ=255)
                                                       tCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAccc                                      >  2:2183541/1‑99 (MQ=255)
                                                         aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc                                   >  2:1105885/1‑100 (MQ=255)
                                                         aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc                                   >  2:905031/1‑100 (MQ=255)
                                                         aGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTc                                   <  2:2378290/100‑1 (MQ=255)
                                                          gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGGCGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                  <  2:625026/100‑1 (MQ=255)
                                                          gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                  >  1:2539321/1‑100 (MQ=255)
                                                          gTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCa                                  <  1:1734535/100‑1 (MQ=255)
                                                            tATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg                                <  1:2179177/100‑1 (MQ=255)
                                                            tATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg                                >  2:1712391/1‑100 (MQ=255)
                                                            tATTTCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAgg                                <  2:319644/100‑1 (MQ=255)
                                                               ttCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATa                             >  1:1656427/1‑100 (MQ=255)
                                                                tCCACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATAc                            >  2:863600/1‑100 (MQ=255)
                                                                 ccACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACg                           >  2:480035/1‑100 (MQ=255)
                                                                 ccACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACg                           >  1:83778/1‑100 (MQ=255)
                                                                  cACTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGa                          >  1:1495337/1‑100 (MQ=255)
                                                                   gatctttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAt                         <  1:1513772/95‑1 (MQ=255)
                                                                   aCTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAt                         <  1:2242940/100‑1 (MQ=255)
                                                                   aCTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAt                         <  1:952192/100‑1 (MQ=255)
                                                                   aCTGGTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAt                         <  1:1436023/100‑1 (MQ=255)
                                                                      ggTTCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTg                      >  1:2513158/1‑100 (MQ=255)
                                                                        ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGAtagatc                    >  2:1513764/1‑95 (MQ=255)
                                                                        ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc                    <  1:527757/100‑1 (MQ=255)
                                                                        ttCGACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTc                    <  2:2044420/100‑1 (MQ=255)
                                                                           gACAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagc                 <  2:1253398/100‑1 (MQ=255)
                                                                            aCAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagca                >  1:2747989/1‑100 (MQ=255)
                                                                            aCAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagca                <  1:1712404/100‑1 (MQ=255)
                                                                             cAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagcag               >  2:1078000/1‑100 (MQ=255)
                                                                             cAACCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTcagcag               >  1:1943204/1‑100 (MQ=255)
                                                                               aCCAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCagcagc              >  2:2258517/1‑99 (MQ=255)
                                                                                ccAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTg            <  1:1630224/100‑1 (MQ=255)
                                                                                 cAGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGa           <  1:1608983/100‑1 (MQ=255)
                                                                                  aGAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAc          <  2:2539303/100‑1 (MQ=255)
                                                                                   gAAAGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAcc         >  2:2746511/1‑100 (MQ=255)
                                                                                    aaaGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccg        <  2:954980/100‑1 (MQ=255)
                                                                                    aaaGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccg        >  1:90940/1‑100 (MQ=255)
                                                                                      aGCACATTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGAccgcc      >  1:1836159/1‑100 (MQ=255)
                                                                                        cacaTTCGGAAAAACGATAAGTTATGAAACCAGACGCACACCGGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGt    >  1:1950727/1‑100 (MQ=255)
                                                                                          caTTCGGAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCg  >  1:262851/1‑100 (MQ=255)
                                                                                                |                                                                                             
TTAGGCACGGCGGCGTTTCGTCAGAAAATCGCCACAGCGATTGCTGAAGGCGTGATCAGTTATTTCCACTGGTTCGACAACCAGAAAGCACATTCGAAAAAGCGATAAGTTATGAAACCCGACGCACACCAGGTTAAACAGTTTCTGCTCAACCTTCAGGATACGATTTGTCAGCAGCTGACCGCCGTCG  >  NC_000913/2553114‑2553303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑