BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,182,712	G→A	A55T (GCC→ACC)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,712	0	G	A	99.1%	376.5 / ‑4.6	113	A55T (GCC→ACC)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (54/57); total (55/58)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 113 total aligned reads displayed.

CGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCAC  >  NC_000913/3182615‑3182808

cGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTAcc                                                                                                <  1:1663388/100‑1 (MQ=255)

  ttCTCGGGCAAAAACCCTCGAACCGCTTACTGTCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGc                                                                                              >  2:1114373/1‑100 (MQ=255)

  ttCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGc                                                                                              <  1:1419024/100‑1 (MQ=255)

   tctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCa                                                                                             >  1:1377991/1‑100 (MQ=255)

    ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCAc                                                                                            <  2:31364/100‑1 (MQ=255)

    ctcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCAc                                                                                            >  1:553329/1‑100 (MQ=255)

     tcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACt                                                                                           <  1:967231/100‑1 (MQ=255)

     tcGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACt                                                                                           >  1:426708/1‑100 (MQ=255)

      cGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTa                                                                                          >  1:352566/1‑100 (MQ=255)

      cGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTa                                                                                          >  2:2567140/1‑100 (MQ=255)

       gggCAAAAACCCTCGGACCGCGTACTGGCGTTTTCGCTCGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTag                                                                                         <  2:276447/100‑1 (MQ=255)

       gggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCCTCTCATTAATGGAAATGCTTCCTACCGCACTag                                                                                         >  2:517305/1‑100 (MQ=255)

       gggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTag                                                                                         <  1:505194/100‑1 (MQ=255)

        ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGGAATGCTTCCTACCGCACTagc                                                                                        >  2:1331257/1‑100 (MQ=255)

        ggCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagc                                                                                        <  2:863053/100‑1 (MQ=255)

          cgatctcccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcag                                                                                      <  2:1459911/94‑1 (MQ=255)

          cAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcag                                                                                      >  2:1318712/1‑100 (MQ=255)

           aaaaaCCCTCGAACCGCTTACTCGCGTTTTCTCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcagc                                                                                     <  2:1945179/100‑1 (MQ=255)

            aaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCt                                                                                    <  1:1374435/100‑1 (MQ=255)

            aaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCt                                                                                    <  2:2541193/100‑1 (MQ=255)

            aaaaCCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTACCAGCt                                                                                    >  1:1929046/1‑100 (MQ=255)

                cccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTagcagagatcg                                                                                >  1:1459918/1‑94 (MQ=255)

                cccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAg                                                                                >  2:1245087/1‑100 (MQ=255)

                cccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAg                                                                                >  2:1912295/1‑100 (MQ=255)

                cccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAg                                                                                <  1:1247869/100‑1 (MQ=255)

                 ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAgg                                                                               >  2:1085404/1‑100 (MQ=255)

                 ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAgg                                                                               <  2:1337646/100‑1 (MQ=255)

                 ccTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAgg                                                                               >  1:1292802/1‑100 (MQ=255)

                  cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGa                                                                              >  2:2439548/1‑100 (MQ=255)

                  cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGa                                                                              >  1:2714062/1‑100 (MQ=255)

                  cTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGa                                                                              >  1:2440398/1‑100 (MQ=255)

                     gAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATg                                                                           <  1:2567158/100‑1 (MQ=255)

                         cGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGc                                                                       >  1:2387795/1‑100 (MQ=255)

                            ttACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCtg                                                                    <  1:1382680/100‑1 (MQ=255)

                              aCTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCtgtg                                                                  >  2:1327846/1‑100 (MQ=255)

                                 ggCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTg                                                               >  1:918713/1‑100 (MQ=255)

                                 ggCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTg                                                               >  1:2662980/1‑100 (MQ=255)

                                 ggCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTg                                                               <  1:261947/100‑1 (MQ=255)

                                  gCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTgg                                                              <  2:863349/100‑1 (MQ=255)

                                   cGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTggg                                                             >  1:1066821/1‑100 (MQ=255)

                                   cGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTggg                                                             <  1:883332/100‑1 (MQ=255)

                                    gTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGt                                                            <  2:1668004/100‑1 (MQ=255)

                                      tttCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTa                                                          <  1:2661425/100‑1 (MQ=255)

                                      tttCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAAAGTCGCCTGTGTTGGGTTa                                                          <  2:1286631/100‑1 (MQ=255)

                                      ggttcgagggTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTa                                                          <  2:194007/92‑1 (MQ=255)

                                         cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                       <  2:1402390/100‑1 (MQ=255)

                                         cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                       <  1:146866/100‑1 (MQ=255)

                                         cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                       >  1:1093107/1‑100 (MQ=255)

                                         cGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATgg                                                       <  1:98597/100‑1 (MQ=255)

                                           cTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGaa                                                     >  2:6293/1‑100 (MQ=255)

                                            tAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAAt                                                    <  1:106809/100‑1 (MQ=255)

                                            tAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAAt                                                    <  1:839759/100‑1 (MQ=255)

                                              ggTTTTGCGGCGGGGATCAGGTAACTCATCTCATTAATGGAAATGCTTCCTACCGCACGAGCAGCTGCAGGAATGTCGCCTCTGTTTGGTTATGGGATGt                                                  >  2:1681429/1‑100 (MQ=255)

                                               gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                 <  1:1620742/100‑1 (MQ=255)

                                               gTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGtt                                                 >  2:1449853/1‑100 (MQ=255)

                                                 tttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTa                                               <  1:2040145/100‑1 (MQ=255)

                                                 tttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTa                                               <  2:407889/100‑1 (MQ=255)

                                                 tttGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTa                                               >  1:518094/1‑100 (MQ=255)

                                                   tGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATc                                             <  1:1177502/100‑1 (MQ=255)

                                                    gcggcgGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCt                                            <  2:187295/100‑1 (MQ=255)

                                                      ggcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTc                                          >  1:1486634/1‑100 (MQ=255)

                                                       gcggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCg                                         >  1:2268877/1‑100 (MQ=255)

                                                        cggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                        <  2:2474894/100‑1 (MQ=255)

                                                        cggGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                        <  1:2840656/100‑1 (MQ=255)

                                                        cggGGATCATGTTGCGCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCgg                                        <  1:2863695/100‑1 (MQ=255)

                                                          gggATCATGTTGCTCATCTCATTAATGGAAGTGCTTCCTTCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTc                                      <  2:2033103/100‑1 (MQ=255)

                                                          gggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTc                                      <  1:2439564/100‑1 (MQ=255)

                                                           ggATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCt                                     >  1:830809/1‑100 (MQ=255)

                                                             aTCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGc                                   <  1:1202220/100‑1 (MQ=255)

                                                              tCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCt                                  >  1:2826567/1‑100 (MQ=255)

                                                              tCATGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCt                                  <  1:1611964/100‑1 (MQ=255)

                                                                aTGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTg                                <  2:2371970/100‑1 (MQ=255)

                                                                 tGTTGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTgg                               <  2:1221156/100‑1 (MQ=255)

                                                                  gTTGCTCATCTCATTAATGGAAATGCTTCCTATCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGc                              <  2:2586633/100‑1 (MQ=255)

                                                                   ttGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTTAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTTTGCTTGGCt                             >  2:1641478/1‑100 (MQ=255)

                                                                    tGCTCATCTCATTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTa                            >  2:460545/1‑100 (MQ=255)

                                                                      ctcatctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAtt                          >  1:1799037/1‑100 (MQ=255)

                                                                       tcatctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAttt                         >  2:188955/1‑100 (MQ=255)

                                                                          tctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTgg                      <  1:906300/100‑1 (MQ=255)

                                                                           ctcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGc                     <  1:372134/100‑1 (MQ=255)

                                                                            tcatTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGcc                    >  2:329132/1‑100 (MQ=255)

                                                                              atTAATGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTg                  >  2:192964/1‑100 (MQ=255)

                                                                                 aaTGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGACTGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAc               >  2:333846/1‑100 (MQ=255)

                                                                                 aaTGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAc               >  1:704927/1‑100 (MQ=255)

                                                                                 aaTGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGAc               >  1:704752/1‑100 (MQ=255)

                                                                                   tGGAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCg             >  2:2726923/1‑100 (MQ=255)

                                                                                     gAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCa           >  2:2675850/1‑100 (MQ=255)

                                                                                     gAAATGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCa           >  1:64457/1‑100 (MQ=255)

                                                                                      aaaTGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCAt          <  2:791714/100‑1 (MQ=255)

                                                                                      aaaTGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCAt          >  1:1216738/1‑100 (MQ=255)

                                                                                        aTGCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGt        <  2:2387780/100‑1 (MQ=255)

                                                                                          gCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTg      >  1:2760634/1‑100 (MQ=255)

                                                                                          gCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTg      <  1:493169/100‑1 (MQ=255)

                                                                                          gCTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTg      >  2:2844663/1‑100 (MQ=255)

                                                                                           cTTCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGc     <  2:830808/100‑1 (MQ=255)

                                                                                            ttCCTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc    <  2:518092/100‑1 (MQ=255)

                                                                                            ctCCTACCGCACTAGCCGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGcc    <  2:1691677/99‑1 (MQ=255)

                                                                                             tCCTACCGCACTAGCTGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca   <  1:2844684/100‑1 (MQ=255)

                                                                                              ccTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcag  >  1:1691690/1‑99 (MQ=255)

                                                                                              ccTACCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcac  >  1:1093094/1‑100 (MQ=255)

CGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGTTGCTCATCTCATTAATGGAAATGCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCAC  >  NC_000913/3182615‑3182808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`