Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,194,516 A→G K551K (AAA→AAG yqiK → PHB family membrane protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,194,5160AG99.0% 365.7 / ‑4.6 103K551K (AAA→AAGyqiKPHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (56/46);  total (56/47)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 105 total aligned reads displayed.

CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGT  >  NC_000913/3194419‑3194612
                                                                                                 |                                                                                                
cGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                                <  1:2756210/100‑1 (MQ=255)
cGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                                >  1:1102416/1‑100 (MQ=255)
cGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGc                                                                                                >  2:2338127/1‑100 (MQ=255)
 gCTGATTGACTCCTTGCTCAATGAAATAGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCa                                                                                               >  2:2434561/1‑100 (MQ=255)
  cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                              <  2:1458816/100‑1 (MQ=255)
   tGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCaga                                                                                             >  2:687866/1‑100 (MQ=255)
    gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                            <  1:2150321/100‑1 (MQ=255)
    gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                            >  2:1483172/1‑100 (MQ=255)
      ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTa                                                                                          >  1:911291/1‑100 (MQ=255)
          cTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAcc                                                                                      >  1:2338280/1‑100 (MQ=255)
            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                    <  1:1331058/100‑1 (MQ=255)
            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                    >  2:423334/1‑100 (MQ=255)
            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                    >  1:1143589/1‑100 (MQ=255)
            ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                    >  1:2155138/1‑100 (MQ=255)
             cTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctc                                                                                   >  2:46642/1‑100 (MQ=255)
              ttGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                  <  2:19345/100‑1 (MQ=255)
              ttGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                  <  2:692579/100‑1 (MQ=255)
              ttGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                  >  1:882075/1‑100 (MQ=255)
              ttGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                  <  2:955715/100‑1 (MQ=255)
               tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCtt                                                                                 >  2:2750247/1‑100 (MQ=255)
                 cTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTcc                                                                               <  2:963598/100‑1 (MQ=255)
                  tCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCa                                                                              <  1:1583254/100‑1 (MQ=255)
                  tCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTCCCa                                                                              <  1:289853/100‑1 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACGACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCa                                                                              <  1:2370772/99‑1 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                             <  2:798157/100‑1 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                             <  1:1423262/100‑1 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                             >  2:350364/1‑100 (MQ=255)
                   cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                             <  2:963540/100‑1 (MQ=255)
                    aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                            <  1:1272152/100‑1 (MQ=255)
                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCGCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                           <  1:2362744/100‑1 (MQ=255)
                     aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGa                                                                           >  2:1666436/1‑100 (MQ=255)
                      tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                          >  1:2576728/1‑100 (MQ=255)
                      tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                          >  1:1193249/1‑100 (MQ=255)
                       gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                         >  1:1478518/1‑100 (MQ=255)
                       gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaat                                                                         <  1:1870147/100‑1 (MQ=255)
                        aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaata                                                                        <  1:46642/100‑1 (MQ=255)
                         aaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaataa                                                                       >  1:2594058/1‑100 (MQ=255)
                         aaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaataa                                                                       >  2:2456097/1‑100 (MQ=255)
                             ggCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATccc                                                                   >  1:814141/1‑100 (MQ=255)
                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                                >  2:940451/1‑100 (MQ=255)
                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                                >  1:2153459/1‑100 (MQ=255)
                                gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                                 >  2:932440/1‑99 (MQ=255)
                                     aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaa                                                           >  2:361830/1‑100 (MQ=255)
                                     aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCaa                                                           >  1:2322612/1‑100 (MQ=255)
                                         ggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAg                                                       >  1:2824030/1‑100 (MQ=255)
                                          gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                      <  1:1442557/100‑1 (MQ=255)
                                          gcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGc                                                      >  1:1809722/1‑100 (MQ=255)
                                           cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                     >  1:2746761/1‑100 (MQ=255)
                                            tCACTGGCGGCATTGACTTCACCCTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   <  1:535830/100‑1 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                     >  2:1920896/1‑98 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   >  2:2610167/1‑100 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   >  1:1173099/1‑100 (MQ=255)
                                             cACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgg                                                   >  2:890399/1‑100 (MQ=255)
                                              aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCGGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                  <  2:2028315/100‑1 (MQ=255)
                                              aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                  <  2:2385376/100‑1 (MQ=255)
                                               cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCgggg                                                 <  2:2322597/100‑1 (MQ=255)
                                                 ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                               >  2:674607/1‑100 (MQ=255)
                                                 ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                               >  2:662633/1‑100 (MQ=255)
                                                 ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                               >  1:2284663/1‑100 (MQ=255)
                                                 ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                               >  1:2551867/1‑100 (MQ=255)
                                                      cATTCACTTCACCCTTAACCTCAACAACACCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                          <  1:204653/100‑1 (MQ=255)
                                                        ttGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAGTAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                        <  2:1706261/100‑1 (MQ=255)
                                                        ttGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                        <  1:1038361/100‑1 (MQ=255)
                                                        ttGACTTCACCCTTAAACTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAACCCCTGGCCCAAAAAGCCGGGGATTTTTtat                                        <  2:612284/100‑1 (MQ=39)
                                                         tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                       <  1:890400/100‑1 (MQ=255)
                                                           aCTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATc                                     <  1:1693633/100‑1 (MQ=255)
                                                            cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                    >  1:2661259/1‑100 (MQ=255)
                                                               cACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg                                 >  1:2328542/1‑100 (MQ=255)
                                                               cACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGAAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCg                                 >  1:1957060/1‑100 (MQ=255)
                                                                aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGt                                >  1:1650081/1‑100 (MQ=255)
                                                                 cccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGtt                               >  2:1193723/1‑100 (MQ=255)
                                                                  ccTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTc                              >  2:2149899/1‑100 (MQ=255)
                                                                    ttAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCg                            <  1:1019570/100‑1 (MQ=255)
                                                                     tAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGc                           <  1:1018738/100‑1 (MQ=255)
                                                                      aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                          <  2:1650068/100‑1 (MQ=255)
                                                                      aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAAAAAAAAAAAAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCg                            <  2:1340225/98‑1 (MQ=21)
                                                                        ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                        >  2:1882733/1‑100 (MQ=255)
                                                                        ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                        >  1:2533995/1‑100 (MQ=255)
                                                                          tCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaa                      <  2:911290/100‑1 (MQ=255)
                                                                             acaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt                   <  2:1722162/100‑1 (MQ=255)
                                                                              caacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg                  >  1:1413883/1‑100 (MQ=255)
                                                                              caacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg                  <  2:2824009/100‑1 (MQ=255)
                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCan               <  1:80022/100‑2 (MQ=255)
                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa               <  1:1649182/100‑1 (MQ=255)
                                                                                 cTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa               >  1:1690235/1‑100 (MQ=255)
                                                                                    ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAAAAACAAGAACAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg            <  2:86782/100‑1 (MQ=21)
                                                                                     cGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAACTGCAAATg            >  2:2480843/1‑99 (MQ=255)
                                                                                      gTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATGATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt          >  2:1557435/1‑100 (MQ=255)
                                                                                       tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGTGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc         <  1:2329450/100‑1 (MQ=255)
                                                                                       tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc         <  2:1143585/100‑1 (MQ=255)
                                                                                       tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc         >  2:2199275/1‑100 (MQ=255)
                                                                                        cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa        >  1:2296948/1‑100 (MQ=255)
                                                                                         gaagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg       <  2:781052/100‑1 (MQ=255)
                                                                                          aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg      >  1:160527/1‑100 (MQ=255)
                                                                                          aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg      <  1:932442/100‑1 (MQ=255)
                                                                                           agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt     <  2:720580/100‑1 (MQ=255)
                                                                                            gaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt    >  1:2857538/1‑100 (MQ=255)
                                                                                            gaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt    >  2:2722991/1‑100 (MQ=255)
                                                                                             aaaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg   <  1:1196590/100‑1 (MQ=255)
                                                                                              aaagGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttgt  >  1:402458/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGT  >  NC_000913/3194419‑3194612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: