Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,541,493 T→C F363L (TTC→CTC)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,541,4930TC99.0% 346.8 / NA 97F363L (TTC→CTC) feoBferrous iron transporter protein B and GTP‑binding protein; membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (44/52);  minor base G (0/1);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTG  >  NC_000913/3541397‑3541589
                                                                                                |                                                                                                
ttCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTcc                                                                                               <  2:1025168/100‑1 (MQ=255)
ttCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTcc                                                                                               >  1:345889/1‑100 (MQ=255)
ttCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTcc                                                                                               >  1:1389237/1‑100 (MQ=255)
ttCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTcc                                                                                               >  1:158383/1‑100 (MQ=255)
   ccGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCctct                                                                                            <  2:1152325/100‑1 (MQ=255)
   ccGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATGAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGctcctc                                                                                             >  1:1745284/1‑99 (MQ=255)
    cGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCctctn                                                                                           <  2:1490123/100‑2 (MQ=255)
      gACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGctcctctcct                                                                                         >  1:1162965/1‑100 (MQ=255)
          ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCtccttcct                                                                                     <  1:1446351/100‑1 (MQ=255)
          ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCtccttcct                                                                                     >  1:171646/1‑100 (MQ=255)
          ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCtccttcct                                                                                     >  2:371540/1‑100 (MQ=255)
          ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCtccttcct                                                                                     <  2:322353/100‑1 (MQ=255)
          ggCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCtccttcct                                                                                     >  1:1304181/1‑100 (MQ=255)
            cTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTg                                                                                   >  2:1033877/1‑100 (MQ=255)
            cTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTg                                                                                   <  2:484877/100‑1 (MQ=255)
            cTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTg                                                                                   >  2:466096/1‑100 (MQ=255)
             tGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGTCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGa                                                                                  <  2:1041691/100‑1 (MQ=255)
             tGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGa                                                                                  <  1:482343/100‑1 (MQ=255)
              gACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAg                                                                                 <  1:2542467/100‑1 (MQ=255)
              gACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAg                                                                                 <  2:1367180/100‑1 (MQ=255)
              gACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAg                                                                                 <  1:1235272/100‑1 (MQ=255)
               aCTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAgg                                                                                <  2:457926/100‑1 (MQ=255)
                cTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGa                                                                               >  1:1437935/1‑100 (MQ=255)
                    cTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTcc                                                                           >  2:1002013/1‑100 (MQ=255)
                    cTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTcc                                                                           >  1:2241624/1‑100 (MQ=255)
                     ttCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGCTTGGCATGCTGTACCTGCTCCTCTCCTTCCTTGAGGACTCCg                                                                          >  1:2314656/1‑100 (MQ=255)
                     ttCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCg                                                                          >  1:2727917/1‑100 (MQ=255)
                       ccTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCggg                                                                        <  1:2221764/100‑1 (MQ=255)
                       ccTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCggg                                                                        <  1:777079/100‑1 (MQ=255)
                        cTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGt                                                                       >  2:2838117/1‑100 (MQ=255)
                           gCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGtata                                                                    <  1:2408166/100‑1 (MQ=255)
                            cccAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTatat                                                                   >  2:1878048/1‑100 (MQ=255)
                            cccAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTatat                                                                   >  1:1428512/1‑100 (MQ=255)
                               agcgCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGgc                                                                <  2:686087/97‑1 (MQ=255)
                               aGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGgc                                                                <  2:560523/100‑1 (MQ=255)
                                  gCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGcgcg                                                             >  2:598519/1‑100 (MQ=255)
                                    cTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTg                                                           >  2:354475/1‑100 (MQ=255)
                                    cTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTg                                                           <  2:345888/100‑1 (MQ=255)
                                     tGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgc                                                          <  2:335329/100‑1 (MQ=255)
                                      gggTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcg                                                         <  1:2216572/100‑1 (MQ=255)
                                      gggTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcg                                                         <  1:937050/100‑1 (MQ=255)
                                       ggTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcgg                                                        >  1:1019864/1‑100 (MQ=255)
                                        gTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcggc                                                       <  2:1236554/100‑1 (MQ=255)
                                         tGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTgcggcg                                                      <  2:1389232/100‑1 (MQ=255)
                                          ggcggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCGTGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGt                                                     >  1:342346/1‑100 (MQ=255)
                                          ggcggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGt                                                     >  1:1295271/1‑100 (MQ=255)
                                           gcggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGtt                                                    >  2:147521/1‑100 (MQ=255)
                                           gcggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGtt                                                    <  1:2291693/100‑1 (MQ=255)
                                             ggcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTtg                                                  <  1:1070609/100‑1 (MQ=255)
                                              gcATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTtgt                                                 <  2:74583/100‑1 (MQ=255)
                                               cATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTtgtg                                                <  1:2474409/100‑1 (MQ=255)
                                               cATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTtgtg                                                >  2:1652082/1‑100 (MQ=255)
                                                tccgatctcGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGa                                               <  1:2222096/92‑1 (MQ=255)
                                                aTTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGa                                               <  1:1137005/100‑1 (MQ=255)
                                                 ttAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGAt                                              <  1:2332942/100‑1 (MQ=255)
                                                 ttAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGAt                                              <  1:2241962/100‑1 (MQ=255)
                                                 ttAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGAt                                              <  1:2169909/100‑1 (MQ=255)
                                                 ttAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGAt                                              <  2:1051093/100‑1 (MQ=255)
                                                  tAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATg                                             >  2:1593475/1‑100 (MQ=255)
                                                   aaCACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATgg                                            <  2:2068207/100‑1 (MQ=255)
                                                   aaCACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATgg                                            <  2:2677521/100‑1 (MQ=255)
                                                   aaCACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATgg                                            <  1:1486112/100‑1 (MQ=255)
                                                    acacCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGa                                           >  2:600642/1‑100 (MQ=255)
                                                     cacCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGan                                          <  2:158382/100‑2 (MQ=255)
                                                     cacCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGAc                                          >  1:2554303/1‑100 (MQ=255)
                                                       ccGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCg                                        >  1:1344546/1‑100 (MQ=255)
                                                       ccGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCg                                        >  1:542154/1‑100 (MQ=255)
                                                        cGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGaagatcgga                                       >  2:2222083/1‑92 (MQ=255)
                                                         gTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTc                                      <  1:167413/100‑1 (MQ=255)
                                                          tgctgcCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCt                                     >  1:979054/1‑100 (MQ=255)
                                                            ctgcCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGa                                   >  2:164308/1‑100 (MQ=255)
                                                            ctgcCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGa                                   <  1:147521/100‑1 (MQ=255)
                                                              gcCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATg                                 >  2:859988/1‑100 (MQ=255)
                                                              gcCACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATAGCGCGTGCGGCGTTTGTGATGGACCGTCTGATg                                 >  2:2830845/1‑100 (MQ=255)
                                                              gcCACTGGTGCCGCAGAGTGGCATGGTGGGCCTGGGCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGGGCGGCGTTTGTGATGGACCGTCTGAt                                  <  2:559829/99‑1 (MQ=255)
                                                               ccACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGc                                <  2:2241611/100‑1 (MQ=255)
                                                               ccACTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGc                                <  1:2414474/100‑1 (MQ=255)
                                                                 aCTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAg                              >  1:995876/1‑100 (MQ=255)
                                                                  cTGGTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAgg                             <  2:2727896/100‑1 (MQ=255)
                                                                     gTGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGgcgc                          >  2:1228408/1‑100 (MQ=255)
                                                                      tGCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGATGACTCCGGGTATATGGCGCCTGCGGCGTTTGTGATGGACCGTCTGATGCAGgcgc                          <  2:773115/99‑1 (MQ=255)
                                                                       gCCGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTg                        >  2:917060/1‑100 (MQ=255)
                                                                        ccGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTgg                       <  1:1830563/100‑1 (MQ=255)
                                                                         cGCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTggg                      >  1:2167956/1‑100 (MQ=255)
                                                                          gCAGATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGc                     <  2:2468537/100‑1 (MQ=255)
                                                                             gATTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTg                  >  1:2487906/1‑100 (MQ=255)
                                                                              aTTGGCATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGc                 <  1:2208596/100‑1 (MQ=255)
                                                                                   cATGATGTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCgggg            <  2:2506256/100‑1 (MQ=255)
                                                                                     tgatgTACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGaa          >  1:2084635/1‑100 (MQ=255)
                                                                                         gTACCTGCTCCTCTCCTTCCTAGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGAGCGTCTGATGCAGGCGCTGGGCTTGCCGGGGACAt        >  2:2699222/1‑98 (MQ=255)
                                                                                          tACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCt     >  2:2091549/1‑100 (MQ=255)
                                                                                          tACCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCt     >  1:1868770/1‑100 (MQ=255)
                                                                                           aCCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCtt    <  2:1019862/100‑1 (MQ=255)
                                                                                           aCCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCtt    <  2:313586/100‑1 (MQ=255)
                                                                                           aCCTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCtt    <  1:1593484/100‑1 (MQ=255)
                                                                                            ccTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCttt   >  1:2233481/1‑100 (MQ=255)
                                                                                            ccTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCttt   <  1:1164896/100‑1 (MQ=255)
                                                                                             cTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTtg  <  2:2803248/100‑1 (MQ=255)
                                                                                             cTGCTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTtg  >  1:950190/1‑100 (MQ=255)
                                                                                                |                                                                                                
TTCCCGGACTGGCTGACTATCTTCCTCGCCCAGGGCCTGGGTGGCGGCATTAACACCGTGCTGCCACTGGTGCCGCAGATTGGCATGATGTACCTGTTCCTCTCCTTCCTTGAGGACTCCGGGTATATGGCGCGTGCGGCGTTTGTGATGGACCGTCTGATGCAGGCGCTGGGCTTGCCGGGGAAATCCTTTG  >  NC_000913/3541397‑3541589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: