Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,410 (T)7→6 100% coding (1280/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4040T.98.0% 464.8 / 2.2 102coding (1274/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base . (50/50);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 118 total aligned reads displayed.

ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCT  >  NC_000913/2470306‑2470498
                                                                                                  |                                                                                              
aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtt                                                                                               <  1:2112314/100‑1 (MQ=255)
  tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttt                                                                                             <  1:684019/100‑1 (MQ=255)
  tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttt                                                                                             <  1:684071/100‑1 (MQ=255)
     aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttta                                                                                          <  1:288155/100‑2 (MQ=255)
       gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        >  1:2234912/1‑97 (MQ=255)
       gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        <  2:323948/100‑4 (MQ=255)
       gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        <  1:2133046/100‑4 (MQ=255)
       gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                        <  1:1402209/100‑4 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       >  1:485977/1‑96 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       >  2:2319024/1‑96 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       >  2:2246676/1‑96 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       >  2:2246666/1‑96 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       <  1:1244518/100‑5 (MQ=255)
        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                       <  2:78423/100‑5 (MQ=255)
          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTta                                                                                    <  1:1940035/100‑1 (MQ=255)
           cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTtat                                                                                   >  2:149001/1‑100 (MQ=255)
            aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGTCCTTTGCTTATACATAACAGTCGTTTTTTAATTtata                                                                                  <  2:1254791/100‑1 (MQ=255)
            aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTtata                                                                                  <  1:53274/100‑1 (MQ=255)
             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGAGCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                 <  2:1262435/100‑1 (MQ=255)
             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATCACAGTCGTTTTTTAATTTATaa                                                                                 <  2:1363581/100‑1 (MQ=255)
                  cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTTTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaataa                                                                            >  2:514370/1‑100 (MQ=255)
                     ggAAGGATATATAGATTTAAGCAAGCATTTATTTTGAATGTAGTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                         <  2:1649718/100‑1 (MQ=255)
                     ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAAtt                                                                          <  2:348268/99‑1 (MQ=255)
                     ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                         <  2:2322377/100‑1 (MQ=255)
                     ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                         <  2:2322155/100‑1 (MQ=255)
                     ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                         <  1:2159473/100‑1 (MQ=255)
                     ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                         <  1:1178604/100‑1 (MQ=255)
                       aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaa                                                                       <  1:2189105/100‑1 (MQ=255)
                        aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaaa                                                                      >  2:1928105/1‑100 (MQ=255)
                          gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                     <  2:1837794/99‑1 (MQ=255)
                             ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                 <  1:1005603/100‑1 (MQ=255)
                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                >  1:800767/1‑100 (MQ=255)
                              taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                >  2:583075/1‑100 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                               <  1:149002/100‑1 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                               >  2:1790527/1‑100 (MQ=255)
                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                               >  2:1794464/1‑100 (MQ=255)
                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTGTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                 >  2:43621/1‑97 (MQ=255)
                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                              <  2:182385/100‑1 (MQ=255)
                                 agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                               <  2:2061776/98‑1 (MQ=255)
                                 agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                             <  2:1612540/100‑1 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  1:1896555/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  2:1551034/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  2:643304/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                            >  2:1059502/1‑100 (MQ=255)
                                  gaTTTAAGCAAGCATTTATTTGGAATGTATTTTACGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGa                                                            >  2:2246530/1‑100 (MQ=255)
                                    tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                          >  1:466561/1‑100 (MQ=255)
                                     ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                         <  2:722122/100‑1 (MQ=255)
                                     ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                          <  1:1861199/99‑1 (MQ=255)
                                       aagcaagcATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                       <  1:1004751/100‑1 (MQ=255)
                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                       >  2:373885/1‑98 (MQ=255)
                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                    <  2:343231/100‑1 (MQ=255)
                                            agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                   >  2:1012974/1‑99 (MQ=255)
                                            agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                  <  1:1311142/100‑1 (MQ=255)
                                            agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                  >  1:879796/1‑100 (MQ=255)
                                             gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                 <  2:2314644/100‑1 (MQ=255)
                                                  tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                            <  1:1489523/100‑1 (MQ=255)
                                                   atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                           <  1:879532/100‑1 (MQ=255)
                                                   atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                           <  1:1824336/100‑1 (MQ=255)
                                                   atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                           <  2:1805736/100‑1 (MQ=255)
                                                     ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAc                                         >  2:281857/1‑100 (MQ=255)
                                                      tGGAATGTATTTTTTTTTTTTTTTTTGCTTATACATAACAGTCGTTTTTTATTTTATAAATTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                        >  2:889977/1‑100 (MQ=255)
                                                       ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                       >  2:1593029/1‑100 (MQ=255)
                                                        gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                      <  1:2305832/100‑1 (MQ=255)
                                                          aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                    <  1:1542211/100‑1 (MQ=255)
                                                           tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                   >  1:1245655/1‑100 (MQ=255)
                                                            gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                  >  1:1204040/1‑100 (MQ=255)
                                                             tccgatctgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAACGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                 <  2:1259392/92‑1 (MQ=255)
                                                             tATTTTTCGTTCAACCTTTGCTTATACATAACCGTCGTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                 >  2:1134606/1‑100 (MQ=255)
                                                               tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                               <  2:947635/100‑1 (MQ=255)
                                                                 tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                             >  2:497449/1‑100 (MQ=255)
                                                                    cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACACTAGCGATGCAAAGCACTAAGTTTAAATTgcg                          >  2:1588105/1‑100 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaagatcgga                         >  1:1259397/1‑93 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAGATTgcgc                         <  1:1236109/100‑1 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                         <  1:1945778/100‑1 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                         >  1:261004/1‑100 (MQ=255)
                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                         >  1:44478/1‑100 (MQ=255)
                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                        <  2:2025335/100‑1 (MQ=255)
                                                                      ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                        <  2:619517/100‑1 (MQ=255)
                                                                       tcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                       >  2:2142149/1‑100 (MQ=255)
                                                                        cgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                      >  1:1133551/1‑100 (MQ=255)
                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                     >  1:1224652/1‑100 (MQ=255)
                                                                              ttGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                >  2:1012731/1‑100 (MQ=255)
                                                                                gCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg              <  1:585026/100‑1 (MQ=255)
                                                                                gCTTATACATAACAGTCGTTTTTTAATTGATAAAATAGTTCAAAGAATTCAAGATAATAACGATGTAAAAACCAAAGCTTAACTTAGACGCCAATAATg               >  2:1254698/1‑99 (MQ=255)
                                                                                  ttATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcg            <  2:2320674/100‑1 (MQ=255)
                                                                                     taaaTAACAGTCGTTTTTTAATTTATAAAATAATTCAAAAAAATCAAGACAATCACGATGTAAAGCACTAAGTTTAAATTACAAGCCAATCATGccgc           >  2:1162942/4‑98 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTTTTTTTTATTATAATTCAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGtcg            >  2:1691248/1‑97 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc         >  1:1748855/1‑100 (MQ=255)
                                                                                     taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc         <  2:2021804/100‑1 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATTAAGACAATAGCGATGTAAAGAACTAAGTTAAAATTGCGCGCCAATCATGGAGCGca        >  2:1694152/1‑99 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac       >  1:338700/1‑100 (MQ=255)
                                                                                       cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac       <  1:514373/100‑1 (MQ=255)
                                                                                        aTAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaat      <  1:1043239/100‑3 (MQ=255)
                                                                                         tAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa     >  1:2191139/1‑100 (MQ=255)
                                                                                         tAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGAGCGCACaa     >  2:818964/1‑100 (MQ=255)
                                                                                          aaCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCATTAAGTTTAAATTGCGCGTCAATCATGGCGCGCACAAg    >  2:1224134/1‑100 (MQ=255)
                                                                                          aaCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg    <  1:67147/100‑1 (MQ=255)
                                                                                           aCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc   <  1:1934817/100‑1 (MQ=255)
                                                                                            cAGTCGTTTTTTATTTTTTAAAATATTTCATATTAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGAGCGCCAATCATGGCGCGCACAAg    >  2:894741/1‑98 (MQ=255)
                                                                                            cAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt  >  2:2223985/1‑100 (MQ=255)
                                                                                                  |                                                                                              
ATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCT  >  NC_000913/2470306‑2470498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: