Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,277,706 (A)7→6 100% coding (370/465 nt) yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,7000A.51.6% 170.2 / 234.3 95coding (364/465 nt)yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base . (26/23);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATT  >  NC_000913/3277601‑3277799
                                                                                                   |                                                                                                   
ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTa                                                                                                     >  1:2230571/1‑100 (MQ=255)
ggTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTa                                                                                                     >  2:2329386/1‑100 (MQ=255)
 gTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaa                                                                                                    >  1:142364/1‑100 (MQ=255)
   gCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                                  <  1:1092171/100‑1 (MQ=255)
   gCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                                  >  1:2279163/1‑100 (MQ=255)
   gCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                                  >  1:952535/1‑100 (MQ=255)
    cTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaa                                                                                                 <  1:1774002/100‑1 (MQ=255)
     tGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                                >  1:2194154/1‑100 (MQ=255)
       gttgTTATCGTCTCTTCTTTAAAAATAAAAAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaaca                                                                                              <  2:2032048/97‑3 (MQ=255)
        tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacag                                                                                             <  2:684775/100‑4 (MQ=255)
        tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa                                                                                             <  1:2107083/100‑1 (MQ=255)
         cGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacaga                                                                                            >  2:941633/1‑96 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGTGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                       <  1:1038721/100‑1 (MQ=255)
             aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                       >  1:1534848/1‑100 (MQ=255)
              tCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGcc                                                                                       <  1:1932906/100‑1 (MQ=255)
              gtgtatcttctTTTGTGATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGTATGAATGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                       <  2:2042787/94‑1 (MQ=255)
                gTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGCTGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtat                                                                                    >  1:579300/1‑100 (MQ=255)
                  ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata                                                                                   <  2:482860/100‑1 (MQ=255)
                  ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAc                                                                                  >  1:409033/1‑100 (MQ=255)
                   tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATAc                                                                                  >  1:1505890/1‑100 (MQ=255)
                    cttcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCg                                                                                >  2:1378933/1‑100 (MQ=255)
                     ttcttTCGTTATAGCGAAAAAGAGAAAGTCATCATTCTTGGATGGATGAACGATGAAAACACTCTTCGCACCTACGGTAAAAAACAGATGCCTATACCGt                                                                               <  2:471799/100‑1 (MQ=255)
                       cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGt                                                                               >  2:1431666/1‑99 (MQ=255)
                          tCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTc                                                                           <  2:1668615/100‑1 (MQ=255)
                           cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCa                                                                          <  2:60962/100‑1 (MQ=255)
                               ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaa                                                                      >  1:945924/1‑100 (MQ=255)
                                 aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                                   <  1:1954363/100‑1 (MQ=255)
                                  gTGAAAAAGAGAAGGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGTTAAAAACACAGATGCCTATACCGTATTCAGCaa                                                                      >  2:663501/1‑97 (MQ=255)
                                  gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                                  >  1:781216/1‑100 (MQ=255)
                                   tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                                  >  2:540811/1‑100 (MQ=255)
                                   tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                                  >  2:2042368/1‑100 (MQ=255)
                                   tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGt                                                                 <  1:898214/100‑1 (MQ=255)
                                    naaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGt                                                                 >  2:2291281/2‑99 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGt                                                                 >  1:1771675/1‑100 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGtt                                                                >  2:1295571/1‑100 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGtt                                                                >  1:316520/1‑100 (MQ=255)
                                    gAAAAAGAGAAAGTCATCATGCGGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCaaaacg                                                                  >  2:1437457/1‑97 (MQ=255)
                                      aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa                                                               <  1:968432/100‑1 (MQ=255)
                                         agagAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaa                                                            >  2:1014962/1‑100 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                          >  1:1188494/1‑100 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                          >  2:569542/1‑100 (MQ=255)
                                          gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCAAATAAAAAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAag                                                          <  2:843039/100‑1 (MQ=255)
                                            gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAagag                                                        >  2:368303/1‑100 (MQ=255)
                                              aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                      <  2:62672/100‑1 (MQ=255)
                                               aGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                      <  1:860312/100‑1 (MQ=255)
                                                 tcatcaTTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACa                                                    <  1:2193182/100‑1 (MQ=255)
                                                  catcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtc                                                  >  2:1494724/1‑100 (MQ=255)
                                                   atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                                 >  1:1409807/1‑100 (MQ=255)
                                                    tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                                 <  1:1085755/100‑1 (MQ=255)
                                                      atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                              >  2:1780361/1‑100 (MQ=255)
                                                      atTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATACCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                              >  1:947812/1‑100 (MQ=255)
                                                       ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                              <  2:184535/100‑1 (MQ=255)
                                                       ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctcc                                              <  1:1476624/100‑1 (MQ=255)
                                                        tCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                            >  1:981083/1‑100 (MQ=255)
                                                         cTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTg                                            >  2:1639521/1‑100 (MQ=255)
                                                           gggATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCg                                         <  1:1600825/100‑1 (MQ=255)
                                                              atggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGAc                                       <  1:655049/100‑1 (MQ=255)
                                                              atggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACt                                      >  1:1264537/1‑100 (MQ=255)
                                                              atggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACt                                      <  1:1469669/100‑1 (MQ=255)
                                                                ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTg                                     <  1:242393/100‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTgg                                    <  1:1137437/100‑1 (MQ=255)
                                                                 gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTgg                                    <  1:963611/100‑1 (MQ=255)
                                                                   tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa                                 <  2:316518/100‑1 (MQ=255)
                                                                    gAACGATGAAAACACTCTGCGCACCTACGGTAAAAACACAGATGCCTATACCGTATTCAGCACAATGCTAAAAAGAGGACATCCTCCTGCCGACTgg                                    >  2:1046136/1‑97 (MQ=255)
                                                                    gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa                                 <  1:217089/100‑1 (MQ=255)
                                                                    gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAACTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa                                 >  1:1146782/1‑99 (MQ=255)
                                                                     aaCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa                                >  1:801349/1‑100 (MQ=255)
                                                                       cGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                             <  1:1378941/100‑1 (MQ=255)
                                                                       cGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                             >  1:1461537/1‑100 (MQ=255)
                                                                        gATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAccc                             >  2:1994232/1‑100 (MQ=255)
                                                                         aTGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                            <  2:2177450/100‑1 (MQ=255)
                                                                          tGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTc                           >  1:1608138/1‑100 (MQ=255)
                                                                           gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCa                          >  1:296167/1‑100 (MQ=255)
                                                                           gAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAc                         >  1:829528/1‑100 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                        <  1:1804890/100‑1 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGCCATCCTCCTGCCGACTGGGAAACCCTCAccc                       >  2:1966986/1‑100 (MQ=255)
                                                                             aaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                       >  1:1986870/1‑100 (MQ=255)
                                                                              aaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg                      <  1:1807371/100‑1 (MQ=255)
                                                                               acacTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                       >  2:2155650/1‑99 (MQ=255)
                                                                                cacTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgag                    <  2:2194135/100‑1 (MQ=255)
                                                                                cacTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgag                    <  1:1727706/100‑1 (MQ=255)
                                                                                 acTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgaga                   <  1:1783583/100‑1 (MQ=255)
                                                                                  ctctGCGCACCTACGGTACAAAAACCGATGCACATACCGTATCCCGCAAAATGTTGCACAGCGGACCTCCCACTGCCGACTGGTaaaac                              >  2:2136906/1‑87 (MQ=255)
                                                                                   tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaa                  <  2:1816803/100‑1 (MQ=255)
                                                                                    ctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                <  2:2069366/100‑1 (MQ=255)
                                                                                    ctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc                <  1:751930/100‑1 (MQ=255)
                                                                                      gcgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa               <  2:539000/100‑1 (MQ=255)
                                                                                        gcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaa            <  2:1124077/100‑1 (MQ=255)
                                                                                        gcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACaga             <  2:121863/99‑1 (MQ=255)
                                                                                         cagcTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAGCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaa            <  1:2222173/97‑1 (MQ=255)
                                                                                         cACCTACGGTAAAAAACAGATGGCTATGCCGCATTCATCAAAATGTTCAAAAGAGGACATCGTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaag           <  2:1585229/100‑1 (MQ=255)
                                                                                          aCCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAACATGTTAAAAAGAGGACATACTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaaga          <  2:782925/100‑1 (MQ=255)
                                                                                           ccTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAgaagaa         >  1:1598814/1‑100 (MQ=255)
                                                                                            cTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa        >  1:905405/1‑100 (MQ=255)
                                                                                             tACGGTAAAAAACAGATGCCTATACCGAATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc       <  1:679844/100‑1 (MQ=255)
                                                                                              aCGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc       >  2:1149038/1‑100 (MQ=255)
                                                                                              aCGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACACTCACCCGAGAAACAGAAGAAAcc      >  2:566257/1‑100 (MQ=255)
                                                                                                 gTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCa    >  1:1790634/1‑100 (MQ=255)
                                                                                                  tAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt   >  1:306045/1‑100 (MQ=255)
                                                                                                   aaaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt  <  2:810667/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATT  >  NC_000913/3277601‑3277799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: