| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,489,197 | C→T | 100% | D322D (GAC→GAT) | aldA → | aldehyde dehydrogenase A, NAD‑linked |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,489,197 | 0 | C | T | 97.5% | 261.6 / ‑2.2 | 81 | D322D (GAC→GAT) | aldA | aldehyde dehydrogenase A, NAD‑linked |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (48/31); total (49/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01 | |||||||||||
TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGC > NC_000913/1489101‑1489296 | tgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATAtt > 1:790965/1‑100 (MQ=255) gtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTg < 1:86585/100‑1 (MQ=255) gtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTg < 1:1403395/100‑1 (MQ=255) tttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGc < 1:1888871/100‑1 (MQ=255) tttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGc > 1:372229/1‑100 (MQ=255) aTGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGAt < 2:1394266/100‑1 (MQ=255) aTGTACAGAAAGGCATTGATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGAt < 2:1757476/100‑1 (MQ=255) gTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGACCGCAACGACATTGCGCTg > 2:2102144/1‑99 (MQ=255) aCAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATgggg > 2:800364/1‑100 (MQ=255) aCAGAAAGGCA‑TTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGc > 1:323125/1‑100 (MQ=255) aGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGc > 2:1201467/1‑99 (MQ=255) gAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCg > 1:1528542/1‑100 (MQ=255) aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGtt > 2:455393/1‑100 (MQ=255) aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGtt < 1:135641/100‑1 (MQ=255) aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGtt > 1:571792/1‑100 (MQ=255) aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGt > 1:1219279/1‑99 (MQ=255) aGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTg > 1:1602185/1‑100 (MQ=255) ggCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGa > 2:2251236/1‑100 (MQ=255) ggCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGa > 1:1233393/1‑100 (MQ=255) cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt < 2:321017/100‑1 (MQ=255) aTTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTa < 1:676298/100‑1 (MQ=255) ttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCTCTGATCGCAACGATATTGCGATGGGGCCGTTGATTa < 2:1057884/98‑1 (MQ=255) ttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAc > 1:1712423/1‑100 (MQ=255) tATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcg > 2:502849/1‑100 (MQ=255) tATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcg > 2:243537/1‑100 (MQ=255) tGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgcc > 1:2049934/1‑100 (MQ=255) aTCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgccgc < 1:1370312/100‑1 (MQ=255) aTCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATCGCGATGGGGCCGTTGATTAAcgccgc > 1:1421152/1‑100 (MQ=255) tCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCcgcg > 1:1445075/1‑100 (MQ=255) tCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACACAACGTTATTGCGATGGGGCCGTTGCTTAACGCcgcg > 2:1589621/1‑100 (MQ=255) aGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggc > 2:2230685/1‑100 (MQ=255) aGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAAAGATATTGCGATGGGGCCGTTGGTTAACGAcgcg > 2:554737/1‑98 (MQ=255) aGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTACAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggc > 2:1261435/1‑100 (MQ=255) gTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggcg > 1:783800/1‑100 (MQ=255) tCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCt > 1:1185675/1‑100 (MQ=255) cGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTg > 2:1468105/1‑100 (MQ=255) tCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAgg < 1:1313077/100‑1 (MQ=255) cTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg < 1:1429089/100‑1 (MQ=255) tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGa < 2:967774/100‑1 (MQ=255) tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGa < 1:1943490/100‑1 (MQ=255) tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGa < 2:482644/100‑1 (MQ=255) gggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAg < 2:1166837/100‑1 (MQ=255) ggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAGAGGGTCGAGc > 1:575940/1‑100 (MQ=255) gCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg > 1:2237349/1‑100 (MQ=255) gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTg > 1:1289342/1‑100 (MQ=255) aTGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTgg > 2:165038/1‑100 (MQ=255) cAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgc > 1:882271/1‑100 (MQ=255) aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCAGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgc > 2:857807/1‑99 (MQ=255) ggCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgc > 2:2088578/1‑100 (MQ=255) gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg < 1:970037/100‑1 (MQ=255) gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg > 1:1579019/1‑100 (MQ=255) gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg > 1:4136/1‑100 (MQ=255) tCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTa < 2:1898137/100‑1 (MQ=255) cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGt > 2:803612/1‑98 (MQ=255) cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag > 2:969953/1‑100 (MQ=255) cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag < 1:1282959/100‑1 (MQ=255) cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag > 1:2354579/1‑100 (MQ=255) aaTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTaga > 2:1704589/1‑100 (MQ=255) aaTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTaga < 2:783797/100‑1 (MQ=255) aTTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaa > 1:1916750/1‑100 (MQ=255) tttGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaag > 1:1977435/1‑100 (MQ=255) tGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAgaagaa > 1:1914446/1‑100 (MQ=255) ggTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGaagaag > 2:226847/1‑100 (MQ=255) gTAACCCCGCTGATCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgg < 1:1219344/100‑1 (MQ=255) gTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgg < 1:455396/100‑1 (MQ=255) gTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgg < 1:1884347/100‑1 (MQ=255) aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg < 2:1712411/100‑1 (MQ=255) aCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg > 2:1702207/1‑99 (MQ=255) aCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGc < 2:2354557/100‑1 (MQ=255) aCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGGCGGGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg > 2:1600580/1‑99 (MQ=255) ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCACAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg > 2:621958/1‑100 (MQ=255) ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg < 2:571789/100‑1 (MQ=255) ccccGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg < 1:2102162/100‑1 (MQ=255) ccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgag < 1:800367/100‑1 (MQ=255) ccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgag > 1:904446/1‑100 (MQ=255) gCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGagagag < 2:1525454/100‑1 (MQ=255) gAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg > 2:1125175/1‑100 (MQ=255) aaCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGc < 2:790962/100‑1 (MQ=255) cAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTc > 1:1526104/1‑100 (MQ=255) cAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTc < 1:1303223/100‑1 (MQ=255) cGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGt < 2:1916736/100‑1 (MQ=255) cATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGc < 1:150249/100‑1 (MQ=255) | TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCGTTCGGTGGC > NC_000913/1489101‑1489296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |