Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,755,639	G→A	100%	intergenic (‑498/‑59)	ydhZ ← / → pykF	uncharacterized protein/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,639	0	G	A	100.0%	281.6 / NA	82	intergenic (‑498/‑59)	ydhZ/pykF	uncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/48);  total (34/48)

GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755544‑1755735                                                                                                |                                                                                                 gagaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGt                                                                                              >  1:508659/1‑100 (MQ=255)     cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATCCCGGATTAGCTTTCCGACAGTgcgc                                                                                          <  1:438173/100‑1 (MQ=255)      acaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                          <  2:68550/99‑1 (MQ=255)      acaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGcc                                                                                         <  2:1526641/100‑1 (MQ=255)       caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                        <  1:1431394/100‑1 (MQ=255)       caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc                                                                                        >  2:1955338/1‑100 (MQ=255)        aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                       <  2:1196137/100‑1 (MQ=255)        aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                       <  1:1939818/100‑1 (MQ=255)            acacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGaaa                                                                                   <  2:2042420/100‑1 (MQ=255)             cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                  >  1:1281609/1‑100 (MQ=255)               caTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCa                                                                                <  2:2216659/100‑1 (MQ=255)                  tCCTCTGCACGCTTTTTTTTTTTTTTTTTTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                      >  2:999348/1‑91 (MQ=255)                   ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                            >  1:966753/1‑100 (MQ=255)                   ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTGTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                            <  1:1145422/100‑1 (MQ=255)                   ccTCTGCACGCTTGTTAGATGTCACCTATCCTTAGAGCTAGGCACCACCACTTTCGTATTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                            <  2:1283730/100‑1 (MQ=255)                    ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt                                                                           >  1:1519251/1‑100 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCAGCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  2:1654059/100‑1 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  1:1638142/100‑1 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  2:1905437/100‑1 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  1:725561/100‑1 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       <  1:682814/100‑1 (MQ=255)                        gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                       >  1:553360/1‑100 (MQ=255)                         cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                      <  2:2290286/100‑1 (MQ=255)                           cGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCAt                                                                    >  1:1622444/1‑100 (MQ=255)                            gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATc                                                                   <  1:999353/100‑1 (MQ=255)                            gCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATc                                                                   >  1:1170093/1‑100 (MQ=255)                              tttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCt                                                                 <  2:1929263/100‑1 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                               >  1:284706/1‑100 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                               <  1:1955352/100‑1 (MQ=255)                                tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                               >  2:2172247/1‑100 (MQ=255)                                    gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa                                                           >  1:836747/1‑100 (MQ=255)                                     aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                          <  2:1493096/100‑1 (MQ=255)                                     aTGTCACCGATCCGTAGAGCGAGGCACCACCACGTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                          <  2:2290656/100‑1 (MQ=255)                                      tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt                                                         <  1:159479/100‑1 (MQ=255)                                       gatctcTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACtt                                                        <  1:1537168/95‑1 (MQ=255)                                         cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                      >  2:227068/1‑100 (MQ=255)                                         cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                      >  2:1671900/1‑100 (MQ=255)                                         cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                      >  2:870419/1‑100 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  2:377649/100‑1 (MQ=255)                                           ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                    <  1:382709/100‑1 (MQ=255)                                            cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTagatc                                                   >  2:1537159/1‑96 (MQ=255)                                             tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                  <  1:272716/100‑1 (MQ=255)                                               tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta                                                <  1:1473805/100‑1 (MQ=255)                                                ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                               >  2:1020638/1‑100 (MQ=255)                                                ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                               >  1:793239/1‑100 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGTAAGCAATTTTCTCCCATCCTTCTCAACTTAaagactaag                                              >  2:1654368/1‑100 (MQ=255)                                                 cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                              >  2:115078/1‑100 (MQ=255)                                                   tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                            >  1:987247/1‑100 (MQ=255)                                                      agcttgtccccaccacTTTCGTAATACCGACGTCGTTTTCCGACATTGCGCCCAGAAACCAAGTTTATCCCATCTTTCTCAACTTAAAGACTAAAACTGt                                         <  2:2233458/91‑1 (MQ=255)                                                       gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTc                                        >  1:1913591/1‑100 (MQ=255)                                                        cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                       <  1:944040/100‑1 (MQ=255)                                                          aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                     >  2:1290437/1‑100 (MQ=255)                                                          aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg                                     <  2:404674/100‑1 (MQ=255)                                                           ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                    <  2:276292/100‑1 (MQ=255)                                                               ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa                                >  1:975566/1‑100 (MQ=255)                                                                caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                               <  1:697667/100‑1 (MQ=255)                                                                  ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                             <  1:561114/100‑1 (MQ=255)                                                                  ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                             <  2:793236/100‑1 (MQ=255)                                                                  ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                             <  1:348141/100‑1 (MQ=255)                                                                   cacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAcc                            <  1:1460531/100‑1 (MQ=255)                                                                      tttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa                         >  2:2030159/1‑100 (MQ=255)                                                                          gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                     >  1:1458942/1‑100 (MQ=255)                                                                           tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                    >  1:1956674/1‑100 (MQ=255)                                                                             aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                  <  1:648234/100‑1 (MQ=255)                                                                             aTACCGGATTCGCTTTCCGACAGTGCGCCAAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                  <  2:350329/100‑1 (MQ=255)                                                                              tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 <  2:1993958/100‑1 (MQ=255)                                                                              tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                 <  2:1191656/100‑1 (MQ=255)                                                                               aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc                >  1:2224731/1‑100 (MQ=255)                                                                                ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa               >  1:1673255/1‑100 (MQ=255)                                                                                 cGGATTCTCTTTCCGACATTTCGCCCAGAAAGCAAGGTTCGCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGATAAAGACCAAAATTGTTTGCa               <  2:374228/99‑1 (MQ=255)                                                                                 cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc              <  1:1636053/100‑1 (MQ=255)                                                                                     ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAGAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc          <  2:455679/100‑1 (MQ=255)                                                                                      tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAATACCAAAATTGTTTGCACCATCg         <  2:806332/100‑1 (MQ=255)                                                                                      tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg         >  1:590207/1‑100 (MQ=255)                                                                                      tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg         >  1:590195/1‑100 (MQ=255)                                                                                         cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      >  1:1137091/1‑100 (MQ=255)                                                                                         cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      <  2:1519242/100‑1 (MQ=255)                                                                                         cTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAc      >  1:1520315/1‑100 (MQ=255)                                                                                          tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAATACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc     <  2:1281603/100‑1 (MQ=255)                                                                                           ttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGTCCAAAATTGTTTGCACCATCGGACCg    <  1:1438592/100‑1 (MQ=255)                                                                                           ttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg    <  2:584052/100‑1 (MQ=255)                                                                                             ccGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGaa  >  2:381561/1‑100 (MQ=255)                                                                                                |                                                                                                 GAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAA  >  NC_000913/1755544‑1755735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑