Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 281,599 C→T 63.8% G129S (GGC→AGC)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,5990CT63.8% 72.2 / 101.8 104G129S (GGC→AGC) yagACP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (16/21);  new base T (27/39);  total (44/60)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 104 total aligned reads displayed.

TGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTA  >  NC_000913/281502‑281696
                                                                                                 |                                                                                                 
tGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCg                                                                                                 <  1:79620/100‑1 (MQ=255)
 ggCAGCGTCCACCGTCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCgg                                                                                                <  2:1600225/100‑1 (MQ=255)
 ggCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCgg                                                                                                >  1:1508808/1‑100 (MQ=255)
  gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGt                                                                                               <  2:898429/100‑1 (MQ=255)
  gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGt                                                                                               <  1:1705167/100‑1 (MQ=255)
   cAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTg                                                                                              >  1:1126086/1‑100 (MQ=255)
     gCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGGTCGAACCGGCCCGTGGCGGGAATGCTCGGTGaa                                                                                            >  1:1644935/1‑100 (MQ=255)
      cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAg                                                                                           >  2:2262893/1‑100 (MQ=255)
       gTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAgc                                                                                          >  1:922236/1‑100 (MQ=255)
          cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGcc                                                                                       >  1:766535/1‑100 (MQ=255)
           aCCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGccc                                                                                      >  1:1657275/1‑100 (MQ=255)
           aCCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGccc                                                                                      >  1:165885/1‑100 (MQ=255)
             cGCTAAAAGGAAAGTGGCTATAAAAATCCATCGACCAAAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGCAATGCTCGGTGGAgc                                                                                          <  2:121892/94‑1 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCgac                                                                                   >  1:985230/1‑98 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                                                   >  1:1424019/1‑100 (MQ=255)
              gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTAGCGGGAATGCCCGGTGAAGCGCCCGgc                                                                                   >  1:1485333/1‑100 (MQ=255)
               ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgca                                                                                  >  1:874078/1‑100 (MQ=255)
               ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgc                                                                                   >  2:1324525/1‑99 (MQ=255)
               ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgca                                                                                  <  1:1844186/100‑1 (MQ=255)
                cAAACGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcag                                                                                 >  1:2307593/1‑100 (MQ=255)
                cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGACCCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcag                                                                                 >  2:2215118/1‑100 (MQ=255)
                cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcag                                                                                 <  2:1846047/100‑1 (MQ=255)
                cAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGgcag                                                                                 >  1:499145/1‑100 (MQ=255)
                   aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGcagcag                                                                              <  1:395382/100‑1 (MQ=255)
                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGCATGCCCGGTGAAGCGCCCGGCAGCAgg                                                                             >  2:1998829/1‑100 (MQ=255)
                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                                             <  2:664134/100‑1 (MQ=255)
                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                                             >  1:1382289/1‑100 (MQ=255)
                    aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                                             <  2:388075/100‑1 (MQ=255)
                     ggAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGc                                                                            <  1:1762432/100‑1 (MQ=255)
                      gAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGcc                                                                           <  2:2024804/100‑1 (MQ=255)
                       aaaGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCa                                                                          <  2:1296858/100‑1 (MQ=255)
                          gTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATgg                                                                       >  1:1681776/1‑100 (MQ=255)
                           tGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGc                                                                      <  2:477106/100‑1 (MQ=255)
                              cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                                                   <  1:847381/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                  <  1:1172264/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCACGGCGGGc                                                                  <  1:1240651/100‑1 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGGGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                  >  1:57885/1‑100 (MQ=255)
                               ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                                                  <  1:145129/100‑1 (MQ=255)
                                cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                                                 >  2:329142/1‑100 (MQ=255)
                                cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                                                 >  2:453057/1‑100 (MQ=255)
                                  tAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCAt                                                               >  2:1126888/1‑100 (MQ=255)
                                   aaaaTCCATCTGCCAGCGGCCGTTGGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc                                                              <  2:1909178/100‑1 (MQ=255)
                                   aaaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATc                                                              <  2:1388830/100‑1 (MQ=255)
                                      aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAg                                                            <  2:614350/99‑1 (MQ=255)
                                      aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                                           <  2:1670412/100‑1 (MQ=255)
                                      aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                                           <  1:989586/100‑1 (MQ=255)
                                        ccATCTGCCAGAGGCGGTTCGGCGCGTCGTTTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATCGCGGGCCATCAg                                                            >  2:688313/1‑97 (MQ=255)
                                        ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                                         <  2:1013719/100‑1 (MQ=255)
                                        ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                                         <  1:1009779/100‑1 (MQ=255)
                                        ccAGCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGt                                                          <  2:1774665/99‑1 (MQ=255)
                                         cATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTa                                                        <  1:322381/100‑1 (MQ=255)
                                          aTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAt                                                       >  1:1888980/1‑100 (MQ=255)
                                          aTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAt                                                       <  2:1194455/100‑1 (MQ=255)
                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                     >  1:63275/1‑100 (MQ=255)
                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGGGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                     <  2:2004709/100‑1 (MQ=255)
                                            cTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAAACGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATgg                                                     >  2:1553649/1‑100 (MQ=255)
                                             tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGa                                                    >  1:173706/1‑100 (MQ=255)
                                                cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACg                                                  >  2:1753940/1‑99 (MQ=255)
                                                cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                                                 <  1:1359376/100‑1 (MQ=255)
                                                cAGAGGCGGGTCGGCGCGTCGTGCTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGACATCAGGTTATGGACgg                                                 <  2:1697773/100‑1 (MQ=255)
                                                   agGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAACGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                                              <  1:1359053/100‑1 (MQ=255)
                                                    ggCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                                             >  1:632825/1‑100 (MQ=255)
                                                    ggCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCt                                             <  1:912765/100‑1 (MQ=255)
                                                     gCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTg                                            <  2:437863/100‑1 (MQ=255)
                                                      cGGTTCGGCGCGTCGAGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGa                                           >  1:262951/1‑100 (MQ=255)
                                                          tCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGc                                       <  1:2262912/100‑1 (MQ=255)
                                                            ggCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                                     >  1:479755/1‑100 (MQ=255)
                                                               gcgTCGTGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa                                  <  2:755484/100‑1 (MQ=255)
                                                                cgtcgtGTTCGAACCGGCCCGTGGAGGGAATGCTCGGTGAAGCGCCCGGCAGCAGTCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc                                   >  2:1562889/1‑98 (MQ=255)
                                                                  tcgtGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg                               <  1:1425443/100‑1 (MQ=255)
                                                                  tcgtGTTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg                               <  1:861744/100‑1 (MQ=255)
                                                                    gtgtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg                             <  1:289633/100‑1 (MQ=255)
                                                                    gggtTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg                             <  2:1126081/98‑1 (MQ=255)
                                                                     tgtTCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt                            <  2:1595402/100‑1 (MQ=255)
                                                                     tgtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtgt                            >  1:606868/1‑100 (MQ=255)
                                                                      gtTCGAACCGGCCCTTTGCGGGAATACTCGGTGAGGCGCCCGGCAACAGGCCATGGCGGGCCAACAGGTCATGGAACGGGCTCAAGCCGAGCAAGgt                              >  2:1387531/1‑97 (MQ=255)
                                                                        tCGAACCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc                         >  1:1896159/1‑100 (MQ=255)
                                                                        tCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcc                         >  2:2291497/1‑100 (MQ=255)
                                                                         cGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGccc                        <  2:733540/100‑1 (MQ=255)
                                                                           aaCCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc                       <  2:1755309/99‑1 (MQ=255)
                                                                            aCCGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg                     >  1:2050346/1‑100 (MQ=255)
                                                                             ccGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTgg                    <  1:1213421/100‑1 (MQ=255)
                                                                             ccGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTg                     <  2:1610953/99‑1 (MQ=255)
                                                                             ccGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTgg                    <  2:643205/100‑1 (MQ=255)
                                                                              cGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATTGTGTGCCCCTGGt                   <  2:1457455/100‑1 (MQ=255)
                                                                              cGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGt                   <  2:752944/100‑1 (MQ=255)
                                                                              cGGCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGt                   <  2:748484/100‑1 (MQ=255)
                                                                               ggCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTc                  >  2:226435/1‑100 (MQ=255)
                                                                                gCCCGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTcc                 <  2:849343/100‑1 (MQ=255)
                                                                                  ccGTGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTc               <  1:980639/100‑1 (MQ=255)
                                                                                    gTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGa             >  2:891469/1‑100 (MQ=255)
                                                                                     tGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGTTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAg            <  2:1254067/100‑1 (MQ=255)
                                                                                     tGGCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAg            <  1:1110540/100‑1 (MQ=255)
                                                                                     tGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAg            <  2:571884/100‑1 (MQ=255)
                                                                                      ggCGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCAGGGCGGGCCATGAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGc           <  2:2307572/100‑1 (MQ=255)
                                                                                        cGGGAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGcc          <  2:884651/99‑1 (MQ=255)
                                                                                         gggAATGCTCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAg        >  1:1156392/1‑100 (MQ=255)
                                                                                             aTGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAgcgc     <  2:36500/99‑1 (MQ=255)
                                                                                              tGCTCGGTGAAGCGCCCGGCAGCGGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCtt   <  2:361934/100‑1 (MQ=255)
                                                                                               gCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTa  >  1:938118/1‑100 (MQ=255)
                                                                                                 |                                                                                                 
TGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTA  >  NC_000913/281502‑281696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: