Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,950,306	C→G	18.3%	G73R (GGC→CGC)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,950,306	0	C	G	18.3%	196.3 / 36.2	76	G73R (GGC→CGC)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (27/35);  new base G (8/6);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTC  >  NC_000913/1950207‑1950405                                                                                                     |                                                                                                    cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGcc                                                                                                     >  2:939440/1‑100 (MQ=255) cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGcc                                                                                                     <  1:2118690/100‑1 (MQ=255)  tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGccc                                                                                                    >  1:1379444/1‑100 (MQ=255)   gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                                   <  2:1222624/100‑1 (MQ=255)   gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                                   <  1:1237024/100‑1 (MQ=255)     gcgGTTGATGATAGTCAGCGAGTTCGCCAGCACTTCGAGTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                 <  2:1044787/100‑1 (MQ=255)     gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                                 <  1:608662/100‑1 (MQ=255)       ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                               <  2:1530711/100‑1 (MQ=38)       ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                               <  2:2102311/100‑1 (MQ=38)       ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                               <  2:350635/100‑1 (MQ=38)       ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                               <  1:1308825/100‑1 (MQ=255)          tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaa                                                                                            <  1:117642/100‑4 (MQ=37)           gatgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGa                                                                                           <  1:963557/100‑1 (MQ=255)           gatgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGGCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaaa                                                                                           <  2:1681617/100‑5 (MQ=25)             tgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAAt                                                                                         >  2:1394905/1‑100 (MQ=255)              gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaaaaaa                                                                                        <  1:757714/100‑8 (MQ=25)                tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                                      >  2:107806/1‑100 (MQ=255)                  gTCAGCGAGGACGCCGGCGCTTGGATTTCGCCGGTCGGCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAg                                                                                     <  2:2131878/99‑1 (MQ=255)                  gTCAGCGAGGACGCCAGTACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                                    >  1:1144411/1‑100 (MQ=255)                  gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                                    <  2:1737940/100‑1 (MQ=255)                    cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCTTCACTTGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  >  2:1917111/1‑100 (MQ=255)                    cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  <  2:2320232/100‑1 (MQ=255)                    cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  >  1:994344/1‑100 (MQ=255)                    cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                                  <  1:1977229/100‑1 (MQ=255)                    cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTAAGGTGCCCGTGACCTGAATGCAGaa                                                                                   >  2:1064594/1‑99 (MQ=255)                       cGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                                               <  1:1643036/100‑1 (MQ=255)                          ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGCCCTGAATGCAGAACTCAtt                                                                             >  2:897638/1‑99 (MQ=255)                          ggACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTa                                                                            >  1:1138449/1‑100 (MQ=255)                            aCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACg                                                                          <  1:473739/100‑1 (MQ=255)                             cGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGc                                                                         <  1:487549/100‑1 (MQ=255)                             cGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGc                                                                         <  1:570823/100‑1 (MQ=255)                              gCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCa                                                                        <  2:1176761/100‑1 (MQ=255)                               ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                       >  2:1747219/1‑100 (MQ=255)                               ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                       >  1:1608358/1‑100 (MQ=255)                               ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                       <  1:633964/100‑1 (MQ=255)                                     cTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCaga                                                                 >  2:55892/1‑100 (MQ=255)                                       tCGCTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGAAGAACTCATTACGCAGTTCa                                                                   >  2:413073/1‑96 (MQ=255)                                          aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                                            >  1:404046/1‑100 (MQ=255)                                           tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                           >  1:1287187/1‑100 (MQ=255)                                           gtttgCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                           >  2:590115/5‑100 (MQ=255)                                             tCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCt                                                         <  1:2295540/100‑1 (MQ=255)                                              cGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCtt                                                        <  1:1599829/100‑1 (MQ=255)                                                ccGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                      <  2:823832/100‑1 (MQ=255)                                                   gTCGCCATATCGCGGTTAATATTTTGTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTa                                                      <  2:319812/97‑1 (MQ=255)                                                      gCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGt                                                >  1:831378/1‑100 (MQ=255)                                                       ccATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTc                                               >  2:12151/1‑100 (MQ=255)                                                       ccATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTc                                               <  1:942582/100‑1 (MQ=255)                                                             cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACg                                         >  1:1510213/1‑100 (MQ=255)                                                             cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACg                                         >  1:53216/1‑100 (MQ=255)                                                                 gTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATcc                                     <  1:1701526/100‑1 (MQ=255)                                                                 gTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATcc                                     <  2:662410/100‑1 (MQ=255)                                                                  ttAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCg                                    <  2:1422075/100‑1 (MQ=255)                                                                    aaTATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGa                                  <  1:12151/100‑1 (MQ=255)                                                                      tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                                <  1:1064599/100‑1 (MQ=255)                                                                      tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                                <  2:580964/100‑1 (MQ=255)                                                                      tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                                <  1:590118/100‑1 (MQ=255)                                                                      tatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                                <  1:735663/100‑1 (MQ=255)                                                                       atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                               >  2:2204728/1‑100 (MQ=255)                                                                            tcaggccACGGGCACCGTAC‑GTGCGCGTGATCTGAATGCAGAACTGATTACGCAGTTCACAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaa                             >  2:1114577/7‑97 (MQ=25)                                                                             tgcGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAAt                         <  1:1847058/98‑1 (MQ=255)                                                                              aggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                        >  1:1937558/3‑100 (MQ=37)                                                                              aggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                        >  1:172706/3‑100 (MQ=37)                                                                               ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                       >  2:1334700/2‑100 (MQ=38)                                                                               cGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                       >  2:937529/1‑100 (MQ=255)                                                                                gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  1:1534185/1‑100 (MQ=38)                                                                                gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  2:1442162/1‑100 (MQ=38)                                                                                gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGACCTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                      >  2:139476/1‑100 (MQ=255)                                                                                  cACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg                    >  1:1307137/1‑100 (MQ=255)                                                                                   atgcgcA‑CGTACGGTGCCCGTGACCTGAATGCAGAGCTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt                     <  2:976675/96‑1 (MQ=255)                                                                                    cgggcaccGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa                  >  2:1255880/8‑100 (MQ=255)                                                                                    cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc                   >  1:1136475/1‑99 (MQ=255)                                                                                    cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa                  <  1:1232516/100‑1 (MQ=255)                                                                                     gcgcA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCAc                 <  1:1416950/100‑1 (MQ=255)                                                                                       gcA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGa               >  2:1952453/1‑100 (MQ=255)                                                                                         a‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATa             <  1:1081225/100‑1 (MQ=255)                                                                                           cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATa             <  1:1747231/99‑1 (MQ=255)                                                                                           cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc            >  1:751724/1‑100 (MQ=255)                                                                                             tACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGAACGATCCGGCTCGAAAAATACCTGCCCGATACCt          >  2:1736632/1‑100 (MQ=255)                                                                                               cGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTc        >  1:1104613/1‑100 (MQ=255)                                                                                                     ccGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTc  >  2:2036963/1‑100 (MQ=255)                                                                                                     |                                                                                                    CTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTCGCGGTC  >  NC_000913/1950207‑1950405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑