Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,516,689	T→C	100%	T382A (ACG→GCG)	yfeA ←	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,516,689	0	T	C	99.2%	347.5 / ‑6.0	95	T382A (ACG→GCG)	yfeA	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (50/44);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCT  >  NC_000913/2516593‑2516784                                                                                                 |                                                                                                gTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACg                                                                                              >  1:1204060/1‑100 (MQ=255) gTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACg                                                                                              >  1:910894/1‑100 (MQ=255)   cGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGc                                                                                            >  2:1666378/1‑100 (MQ=255)    gCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCACCGGCAGCAGCGCACGGCa                                                                                           <  2:1523417/100‑1 (MQ=255)       cTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAt                                                                                        <  1:1529546/100‑1 (MQ=255)        ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCCATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAtt                                                                                       <  2:2348030/100‑1 (MQ=255)        ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAtt                                                                                       >  2:1320874/1‑100 (MQ=255)        ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAtt                                                                                       <  2:2350501/100‑1 (MQ=255)         tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                      >  2:150086/1‑100 (MQ=255)           cGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAg                                                                                    >  1:2344244/1‑100 (MQ=255)             tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                  <  1:743953/100‑1 (MQ=255)             tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                  >  2:467560/1‑100 (MQ=255)              ttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCgg                                                                                 <  2:1566950/100‑1 (MQ=255)              ttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCgg                                                                                 <  2:378554/100‑1 (MQ=255)               tCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGa                                                                                <  1:2077452/100‑1 (MQ=255)                ccGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGAt                                                                               >  2:582062/1‑100 (MQ=255)                 cGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATa                                                                              <  2:2220265/100‑1 (MQ=255)                  ggCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATAc                                                                             >  2:831971/1‑100 (MQ=255)                     ccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGt                                                                          <  2:2249323/100‑1 (MQ=255)                      cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                         <  1:1666389/100‑1 (MQ=255)                      cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                         <  2:178266/100‑1 (MQ=255)                       gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGa                                                                        <  1:2211761/100‑1 (MQ=255)                       gCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGa                                                                        >  2:419765/1‑100 (MQ=255)                        cTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGaa                                                                       <  1:1858450/100‑1 (MQ=255)                        cTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGaa                                                                       <  1:27097/100‑1 (MQ=255)                         tCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAc                                                                      >  1:1510755/1‑100 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   <  2:2235537/100‑1 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   <  2:2267710/100‑1 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   >  2:1578734/1‑100 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   >  2:1070450/1‑100 (MQ=255)                            gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAACGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                   >  1:1616578/1‑100 (MQ=255)                               cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                <  1:970956/100‑1 (MQ=255)                               cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                >  2:37409/1‑100 (MQ=255)                                  aCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatcat                                                             >  1:2353987/1‑100 (MQ=255)                                  aCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatc                                                               >  2:65736/1‑98 (MQ=255)                                   cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                            <  2:519525/100‑1 (MQ=255)                                   cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                            >  2:993998/1‑100 (MQ=255)                                   cagaagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCATCGCACGTCAAATTGAGCAGATACAGTGAACgcgccacact                                                            >  2:466962/1‑94 (MQ=255)                                      cagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAg                                                         <  2:1133705/100‑1 (MQ=255)                                      cagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAg                                                         <  1:1618797/100‑1 (MQ=255)                                      cagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAg                                                         <  1:304798/100‑1 (MQ=255)                                          tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                     >  1:357686/1‑100 (MQ=255)                                           cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTa                                                    >  2:2321188/1‑100 (MQ=255)                                           cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTa                                                    >  1:2179516/1‑100 (MQ=255)                                               aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATga                                                >  1:447718/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               <  1:2072484/100‑1 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  1:751960/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  1:144839/1‑100 (MQ=255)                                                cccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgac                                               >  1:831021/1‑100 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              <  2:1217789/100‑1 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              <  1:1698734/100‑1 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              <  1:2131902/100‑1 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              >  2:455057/1‑100 (MQ=255)                                                 ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                              <  1:923042/100‑1 (MQ=255)                                                  cGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGCGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacga                                             >  2:378585/1‑100 (MQ=255)                                                   ggCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacgac                                            <  1:230010/100‑1 (MQ=255)                                                      aaTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCa                                         >  2:1388506/1‑100 (MQ=255)                                                      aaTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCa                                         >  1:796535/1‑100 (MQ=255)                                                      aaTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCa                                         >  1:476527/1‑100 (MQ=255)                                                       aTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCAt                                        >  2:244046/1‑100 (MQ=255)                                                       aTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCAt                                        >  2:243764/1‑100 (MQ=255)                                                          gATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATaaa                                     >  1:375649/1‑100 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAAc                                    >  2:962209/1‑99 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                   >  2:102206/1‑100 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                   >  1:145690/1‑100 (MQ=255)                                                            tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                   >  2:2178180/1‑100 (MQ=255)                                                              cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                 >  1:1247681/1‑100 (MQ=255)                                                              cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                 >  1:1884944/1‑100 (MQ=255)                                                              cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                 <  2:1145501/100‑1 (MQ=255)                                                                 cTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGa                              >  2:639699/1‑100 (MQ=255)                                                                  ttttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAt                             <  1:443173/100‑1 (MQ=255)                                                                  ttttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAt                             <  2:1169341/100‑1 (MQ=255)                                                                     tCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTAt                          <  2:910890/100‑1 (MQ=255)                                                                     tCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTAt                          <  2:843490/100‑1 (MQ=255)                                                                      cGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATACACTCAAGATTATc                         >  2:2218131/1‑100 (MQ=255)                                                                      cGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATc                         >  1:1539721/1‑100 (MQ=255)                                                                       gTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCCTTAAGCCGTAATGACGACTCATAAACTCAAGATTCTCa                        >  1:2312958/1‑100 (MQ=255)                                                                         tttCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAAt                      <  2:211918/100‑1 (MQ=255)                                                                           tCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgc                    >  1:1571838/1‑100 (MQ=255)                                                                             cTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcgc                  >  1:816263/1‑100 (MQ=255)                                                                              tGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgca                 <  2:1824028/100‑1 (MQ=255)                                                                              tGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgca                 >  1:2129194/1‑100 (MQ=255)                                                                              tGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgca                 <  2:604681/100‑1 (MQ=255)                                                                                cATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcagg               <  1:1399768/100‑1 (MQ=255)                                                                                cATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcagg               <  2:357683/100‑1 (MQ=255)                                                                                 aTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggc              <  2:509083/100‑1 (MQ=255)                                                                                   cAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggcn             <  2:2247375/99‑2 (MQ=255)                                                                                   cAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGAATCATAAACTCAAGATTATCAATTCgcaggcag            >  2:521567/1‑100 (MQ=255)                                                                                    aaCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGgcagc           >  1:194975/1‑100 (MQ=255)                                                                                    aaCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGgcagc           <  1:150087/100‑1 (MQ=255)                                                                                    aaCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGgcagc           <  1:1689462/100‑1 (MQ=255)                                                                                       ggCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaaa        <  1:467563/100‑1 (MQ=255)                                                                                         cagcagCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCCTAAACTCAAGATTATCAATGCGCAGGCAGCaaaa       >  2:311342/1‑99 (MQ=255)                                                                                            cagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActc   <  1:283037/100‑1 (MQ=255)                                                                                             cgcgcACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATCCGCAAGCAGGAAAAAcgct  >  1:533027/2‑100 (MQ=255)                                                                                             agcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActct  <  1:2031310/100‑1 (MQ=255)                                                                                                 |                                                                                                GTCGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCT  >  NC_000913/2516593‑2516784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑