Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,542,842 C→T 15.4% Q39* (CAA→TAA)  feoC → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,542,8420CT15.4% 243.8 / 28.5 96Q39* (CAA→TAA) feoCputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (45/36);  new base T (5/10);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTGTCTGC  >  NC_000913/3542743‑3542941
                                                                                                   |                                                                                                   
cAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGcaac                                                                                                     >  1:78866/1‑100 (MQ=255)
cAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGcaac                                                                                                     <  1:1550038/100‑1 (MQ=255)
cAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGcaac                                                                                                     >  2:1126180/1‑100 (MQ=255)
cAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGcaac                                                                                                     >  2:1466286/1‑100 (MQ=255)
    tGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACt                                                                                                 <  1:274138/100‑1 (MQ=255)
    tGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACt                                                                                                 <  1:1142270/100‑1 (MQ=255)
     gcgcGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTg                                                                                                <  2:1047174/100‑1 (MQ=255)
     gcgcGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTg                                                                                                >  1:1440333/1‑100 (MQ=255)
     gcgcGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTg                                                                                                <  2:1193233/100‑1 (MQ=255)
     gcgcGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTg                                                                                                >  2:1494691/1‑100 (MQ=255)
      cgcgATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTgg                                                                                               <  2:1570734/100‑1 (MQ=255)
      cgcgATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTgg                                                                                               <  1:499806/100‑1 (MQ=255)
       gcgATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGa                                                                                              >  1:635114/1‑100 (MQ=255)
        cgATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGaa                                                                                             <  1:97811/100‑1 (MQ=255)
          aTTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAg                                                                                           <  2:1018362/100‑1 (MQ=255)
             tGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTAt                                                                                        >  1:2264159/1‑100 (MQ=255)
              gatGGCGTTACGGGGCCGTATTCGAGCGGCCCAGATAAGCCAGACATTGAACACTCGACAGCAAATGATTAACGCCATGCTGCAATAACTGGAAAg                                                                                           <  2:2242936/94‑1 (MQ=255)
              gCTGGCGTTATGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATg                                                                                       >  2:809429/1‑100 (MQ=255)
              gCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATg                                                                                       >  1:482159/1‑100 (MQ=255)
              gCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATg                                                                                       >  1:1146616/1‑100 (MQ=255)
              gCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATg                                                                                       >  2:1413787/1‑100 (MQ=255)
              gCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATg                                                                                       >  1:2054217/1‑100 (MQ=255)
                tGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATggg                                                                                     <  2:716505/100‑1 (MQ=255)
                tGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATggg                                                                                     <  2:247791/100‑1 (MQ=255)
                 ggCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGc                                                                                    >  1:1441753/1‑100 (MQ=255)
                    nttACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTCACGCCATGCTGCAATAACTGGAAAGTATGGGCaaa                                                                                 >  2:852960/2‑100 (MQ=255)
                    gTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCaaa                                                                                 >  1:1713806/1‑100 (MQ=255)
                      tACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGc                                                                               <  1:1060512/100‑1 (MQ=255)
                        cGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCg                                                                             >  1:1988108/1‑100 (MQ=255)
                         ggggCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGt                                                                            <  1:1668801/100‑1 (MQ=255)
                            gCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCg                                                                         <  2:944112/100‑1 (MQ=255)
                            gCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCg                                                                         <  1:181337/100‑1 (MQ=255)
                            gCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTCCAACAACTGGAAAGTATGGGCAAAGCCGTGCg                                                                         <  1:483739/100‑1 (MQ=255)
                              cGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGa                                                                       >  1:710355/1‑100 (MQ=255)
                               gTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGAt                                                                      <  2:589303/100‑1 (MQ=255)
                                tATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATACTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGAtt                                                                     >  2:163642/1‑100 (MQ=255)
                                 aTGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTc                                                                    >  2:1978368/1‑100 (MQ=255)
                                   ggAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAg                                                                  >  2:505132/1‑100 (MQ=255)
                                    gAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAgg                                                                 <  1:262356/100‑1 (MQ=255)
                                    gAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAgg                                                                 <  2:1013787/100‑1 (MQ=255)
                                     aaGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGga                                                                >  2:709624/1‑100 (MQ=255)
                                     aaGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGga                                                                <  2:936240/100‑1 (MQ=255)
                                      aGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaa                                                               <  2:2181190/100‑1 (MQ=255)
                                      aGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaa                                                               >  1:810409/1‑100 (MQ=255)
                                       gCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaag                                                              <  1:926725/100‑1 (MQ=255)
                                         ggCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaagaa                                                            >  2:935857/1‑100 (MQ=255)
                                         ggCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaagaa                                                            >  2:2238131/1‑100 (MQ=255)
                                         ggCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGgaagaa                                                            >  2:2306011/1‑100 (MQ=255)
                                          gCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAAc                                                           <  1:2098993/100‑1 (MQ=255)
                                           cccAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAAcc                                                          <  2:261139/100‑1 (MQ=255)
                                           cccAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAAcc                                                          >  2:1294549/1‑100 (MQ=255)
                                            ccAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCt                                                         <  2:624047/100‑1 (MQ=255)
                                             cAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTg                                                        <  2:1013167/100‑1 (MQ=255)
                                             cAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTg                                                        <  2:1720185/100‑1 (MQ=255)
                                               gagaaGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGAc                                                      <  2:1120986/97‑1 (MQ=255)
                                               gATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGAc                                                      <  1:332469/100‑1 (MQ=255)
                                                  aaGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGc                                                   <  2:308716/100‑1 (MQ=255)
                                                   aGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCt                                                  >  1:982702/1‑100 (MQ=255)
                                                    gccgggcATTCAACACTCCACTGGCGAAGATTTACGCCATGCTGCAACAACTGGACAGTAGGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCt                                                  >  2:2095760/7‑99 (MQ=255)
                                                    gCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTg                                                 >  1:2146528/1‑100 (MQ=255)
                                                    gCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTg                                                 >  2:1631156/1‑100 (MQ=255)
                                                    gCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTg                                                 <  2:635111/100‑1 (MQ=255)
                                                    gCCAGACATTAAAAACTCCAAAACCAATGATTAACGCCATGCTGCAACAACTGGAAAGAATGGCCAAAGCCGTGCGGATTCAGGAATAACCTGACgg                                                    >  2:192466/1‑97 (MQ=255)
                                                      cAGACATTGAACCCTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGcc                                               <  2:1283001/100‑1 (MQ=255)
                                                      cAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGcc                                               <  2:2321018/100‑1 (MQ=255)
                                                      cAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGcc                                               >  1:294080/1‑100 (MQ=255)
                                                          cATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCctctc                                           >  1:1805644/1‑100 (MQ=255)
                                                           aTTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCctctct                                          <  2:1793520/100‑1 (MQ=255)
                                                            ttGAACACTCCACAGCCAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTg                                         <  1:163643/100‑1 (MQ=255)
                                                              gAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGc                                       >  2:338666/1‑100 (MQ=255)
                                                               aaCACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCa                                      >  1:2018180/1‑100 (MQ=255)
                                                                acacTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAg                                     <  1:380332/100‑1 (MQ=255)
                                                                acacTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAg                                     <  2:1124979/100‑1 (MQ=255)
                                                                 cacTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGt                                    >  1:2184748/1‑100 (MQ=255)
                                                                  acTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGtt                                   >  2:1343435/1‑100 (MQ=255)
                                                                   cTCCACAGCCAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTg                                  >  1:705134/1‑100 (MQ=255)
                                                                   cTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGt                                    <  2:619271/98‑1 (MQ=255)
                                                                   cTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTg                                  >  2:1599209/1‑100 (MQ=255)
                                                                    tCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGt                                 <  2:2002632/100‑1 (MQ=255)
                                                                     ccACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGt                                 >  2:330414/1‑99 (MQ=255)
                                                                     ccACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTa                                >  2:2302240/1‑100 (MQ=255)
                                                                       acaGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTaaa                              >  1:241877/1‑100 (MQ=255)
                                                                          gCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGc                           >  1:2068885/1‑100 (MQ=255)
                                                                           ccAATGATTAACGCCATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCt                          >  2:1252292/1‑100 (MQ=255)
                                                                            cAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTg                         >  1:1695239/1‑100 (MQ=255)
                                                                             aaTGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGc                        <  1:1413795/100‑1 (MQ=255)
                                                                              aTGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGcc                       <  2:2018165/100‑1 (MQ=255)
                                                                                   tAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCggaa                  >  2:1817319/1‑100 (MQ=255)
                                                                                    aaCGCCATGCTGCAACAACTGGAAAGTGTGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCggaag                 >  1:201545/1‑100 (MQ=255)
                                                                                     aCGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCggaagg                >  2:1733765/1‑100 (MQ=255)
                                                                                       gCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCggaaggaa              >  1:401710/1‑100 (MQ=255)
                                                                                        ccATGCTGCAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCggaaggaan             <  1:852964/100‑2 (MQ=255)
                                                                                        ccATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGaaa             <  1:829887/100‑1 (MQ=255)
                                                                                          aTGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAg           <  1:323754/100‑1 (MQ=255)
                                                                                             ctgcAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCt        >  1:2307663/1‑100 (MQ=255)
                                                                                              tgcAATAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTg       <  1:709627/100‑1 (MQ=255)
                                                                                              tgcAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTg       >  2:2341861/1‑100 (MQ=255)
                                                                                               gcAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTGt      >  2:526926/1‑100 (MQ=255)
                                                                                                caacaaCTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTGTc     <  1:300291/100‑1 (MQ=255)
                                                                                                   caacTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTGTCTgc  >  2:1920412/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAGGTGCGCGATTTGCTGGCGTTACGGGGCCGTATGGAAGCGGCCCAGATAAGCCAGACATTGAACACTCCACAGCCAATGATTAACGCCATGCTGCAACAACTGGAAAGTATGGGCAAAGCCGTGCGGATTCAGGAAGAACCTGACGGCTGCCTCTCTGGCAGTTGTAAAAGCTGCCCGGAAGGAAAAGCCTGTCTGC  >  NC_000913/3542743‑3542941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: