Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	953,802	A→G	100%	V222A (GTA→GCA)	focA ←	formate channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,802	0	A	G	100.0%	293.2 / NA	81	V222A (GTA→GCA)	focA	formate channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/47);  total (34/47)

GTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953705‑953897                                                                                                  |                                                                                                gTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCa                                                                                               <  1:1312752/100‑1 (MQ=255)  ttATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                              <  1:34566/100‑1 (MQ=255)   tgaccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGTTGCGAAGTCGCGGATTGCaa                                                                                              >  2:161652/6‑99 (MQ=255)   tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                             >  1:2269492/1‑100 (MQ=255)    aTCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATa                                                                                            >  1:2330676/1‑100 (MQ=255)     tCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                           <  1:624936/100‑1 (MQ=255)        gTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCa                                                                                        <  1:82301/100‑1 (MQ=255)        gTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCa                                                                                        >  1:1456315/1‑100 (MQ=255)          gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  2:1102376/1‑100 (MQ=255)          gatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATc                                                                                      >  1:1778776/1‑100 (MQ=255)           atgaAATTCATCACGGTAAGATGAGAAAAAATTTCCGATGAAAAAACGAATGCGGTCCAAAATTCCGGGGATGCGAAATCGCGGATTGCAATACCCATCg                                                                                     >  2:1756379/1‑100 (MQ=255)             gaAATTCATCACGGTCAGGTGAGAAAAATTTTCTGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCggg                                                                                   >  2:1336335/1‑100 (MQ=255)             gaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCggg                                                                                   >  1:921306/1‑100 (MQ=255)                aTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATc                                                                                <  2:179018/100‑1 (MQ=255)                   catcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATa                                                                             >  1:825571/1‑100 (MQ=255)                   catcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATa                                                                             <  1:1901909/100‑1 (MQ=255)                    atcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaa                                                                            <  1:473574/100‑1 (MQ=255)                     tcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaaa                                                                           <  2:2285261/100‑1 (MQ=255)                      caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                          <  1:240616/100‑1 (MQ=255)                      caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                          <  1:1445928/100‑1 (MQ=255)                          gTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGt                                                                      >  1:390844/1‑100 (MQ=255)                            cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                    >  2:1270349/1‑100 (MQ=255)                             aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  1:554975/100‑1 (MQ=255)                             aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                   <  2:947950/100‑1 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  1:345951/1‑100 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  1:2146841/1‑100 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:840629/100‑1 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:955044/100‑1 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  <  1:2024589/100‑1 (MQ=255)                              ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                  >  2:1950234/1‑100 (MQ=255)                                   gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  2:2274390/1‑100 (MQ=255)                                   gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             >  2:588951/1‑100 (MQ=255)                                   gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                             <  2:481074/100‑1 (MQ=255)                                      aaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtgt                                                          >  1:1953694/1‑100 (MQ=255)                                         ttttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCt                                                       <  1:2333903/100‑1 (MQ=255)                                          tttCCGGTGCAGAACCTACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTc                                                      <  2:1380504/100‑1 (MQ=255)                                          tttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTc                                                      <  1:1249122/100‑1 (MQ=255)                                          tgtCCGGTGCAGCACCGACTGCGGTCCAAAATTCCGGGGATGCAAAATCGCGGATTGCAATACCCATCGGGATCACAAACATGTTTGCGATACTGTGCTc                                                      <  2:916938/98‑1 (MQ=255)                                           ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATGGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                     <  2:1025815/100‑1 (MQ=255)                                            tCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaa                                                    <  1:1461439/100‑1 (MQ=255)                                              cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTTCGATACTGTGCTCaaa                                                   >  1:93597/1‑99 (MQ=255)                                              cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaaa                                                  >  2:744767/1‑100 (MQ=255)                                              cGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaaa                                                  >  1:124714/1‑100 (MQ=255)                                               ggTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                 >  1:1820650/1‑100 (MQ=255)                                                 tGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCg                                               >  1:415599/1‑100 (MQ=255)                                                  gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              <  1:1617982/100‑1 (MQ=255)                                                  gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              >  2:2331409/1‑100 (MQ=255)                                                  gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              >  1:1425989/1‑100 (MQ=255)                                                  gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                              >  2:1855357/1‑100 (MQ=255)                                                   cAGAACCGACTGCGGTCCAAAATTCCGCGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCt                                             <  2:1129822/100‑1 (MQ=255)                                                     gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                           <  1:1170951/100‑1 (MQ=255)                                                      aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  2:1059929/100‑1 (MQ=255)                                                      aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                          <  1:472402/100‑1 (MQ=255)                                                        ccGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaa                                        <  1:489721/100‑1 (MQ=255)                                                         cGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaac                                       <  1:151480/100‑1 (MQ=255)                                                          gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  1:180622/1‑100 (MQ=255)                                                          gACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaaca                                      >  1:248027/1‑100 (MQ=255)                                                           aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                     <  1:2135872/100‑1 (MQ=255)                                                            cTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaa                                    >  1:2239216/1‑100 (MQ=255)                                                             tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:661975/100‑1 (MQ=255)                                                             tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                   <  1:1678806/100‑1 (MQ=255)                                                                 gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               <  1:593094/100‑1 (MQ=255)                                                                 gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  2:821568/1‑100 (MQ=255)                                                                 gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                               >  2:45372/1‑100 (MQ=255)                                                                    cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            >  1:373466/1‑100 (MQ=255)                                                                    cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                            <  1:1844210/100‑1 (MQ=255)                                                                         ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                       >  2:792636/1‑100 (MQ=255)                                                                          tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                      <  1:1448898/100‑1 (MQ=255)                                                                           ccGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcag                     <  1:1961036/100‑1 (MQ=255)                                                                                 aTGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCAt               <  1:1307417/100‑1 (MQ=255)                                                                                  tGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATa              <  2:2162255/100‑1 (MQ=255)                                                                                    cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATaa             <  1:1804270/99‑1 (MQ=255)                                                                                    cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt            <  2:1456307/100‑1 (MQ=255)                                                                                    cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt            <  1:871799/100‑1 (MQ=255)                                                                                        gTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAAc        <  1:957681/100‑1 (MQ=255)                                                                                         tCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACg       <  2:2330654/100‑1 (MQ=255)                                                                                          cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc      >  2:1716079/1‑100 (MQ=255)                                                                                            cgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCtt    <  1:451969/100‑1 (MQ=255)                                                                                            cgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCtt    <  2:907010/100‑1 (MQ=255)                                                                                              gATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTg  <  1:1187762/100‑1 (MQ=255)                                                                                              gATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTg  <  1:1122229/100‑1 (MQ=255)                                                                                                  |                                                                                                GTTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTG  >  NC_000913/953705‑953897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑