Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,146,997	G→A	55.0%	T68T (ACG→ACA)	yceD →	DUF177 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,997	0	G	A	55.0%	17.6 / 120.0	89	T68T (ACG→ACA)	yceD	DUF177 family protein
Reads supporting (aligned to +/- strand):  ref base G (19/21);  new base A (24/25);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

CGAATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146898‑1147093                                                                                                    |                                                                                                 cGAATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACg                                                                                                  <  2:1775059/100‑1 (MQ=255)    aTCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTa                                                                                               >  1:1218598/1‑100 (MQ=255)     tCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTaa                                                                                              <  2:217871/100‑1 (MQ=255)      ccGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAc                                                                                             >  2:2084255/1‑100 (MQ=255)      ccGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAAc                                                                                             >  2:1905013/1‑100 (MQ=255)       cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACg                                                                                            <  1:1999120/100‑1 (MQ=255)       cGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACg                                                                                            >  2:1049498/1‑100 (MQ=255)          aGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTc                                                                                         >  2:698119/1‑100 (MQ=255)             cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAg                                                                                      <  1:346918/100‑1 (MQ=255)             cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAg                                                                                      <  1:808736/100‑1 (MQ=255)             cAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAg                                                                                      >  1:1578312/1‑100 (MQ=255)              aGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAgt                                                                                     >  1:257532/1‑100 (MQ=255)              aGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgt                                                                                     >  2:1077066/1‑100 (MQ=255)               gtgtGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAgtg                                                                                    <  2:1796285/100‑1 (MQ=255)               gtgtGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAgtg                                                                                    >  1:668132/1‑100 (MQ=255)                   ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAg                                                                                >  1:321511/1‑100 (MQ=255)                   ggACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAg                                                                                >  2:990134/1‑100 (MQ=255)                    gACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGc                                                                               >  1:947188/1‑100 (MQ=255)                        gTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTg                                                                           >  1:1272952/1‑100 (MQ=255)                        gTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTg                                                                           <  1:576494/100‑1 (MQ=255)                          gATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCg                                                                         >  1:1414542/1‑100 (MQ=255)                           aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  2:521584/100‑1 (MQ=255)                           aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  1:699565/100‑1 (MQ=255)                           aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        <  2:2008059/100‑1 (MQ=255)                           aTGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCgg                                                                        >  2:1062348/1‑100 (MQ=255)                            tgtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCggg                                                                       <  2:646897/100‑1 (MQ=255)                             gtgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGa                                                                      <  1:1077071/100‑1 (MQ=255)                              tgGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGaa                                                                     <  2:1599435/100‑1 (MQ=255)                               ggAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    <  1:1026864/100‑1 (MQ=255)                               ggAATGCTCCACGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    >  1:30002/1‑100 (MQ=255)                               ggAATGCTCCACGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAg                                                                    >  1:318584/1‑100 (MQ=255)                                gAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGc                                                                   <  2:1578302/100‑1 (MQ=255)                                   tGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGt                                                                <  2:197138/100‑1 (MQ=255)                                    gCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGtt                                                               >  2:801698/1‑100 (MQ=255)                                     cTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGttt                                                              >  2:180863/1‑100 (MQ=255)                                       ccATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTAc                                                            >  1:452801/1‑100 (MQ=255)                                        cATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACt                                                           <  1:814291/100‑1 (MQ=255)                                         aTGTCGTTCGCTGTCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtc                                                          <  2:1382980/100‑1 (MQ=255)                                         aTGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtc                                                          <  1:2103339/100‑1 (MQ=255)                                         aTGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtc                                                          <  2:769850/100‑1 (MQ=255)                                          tGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtca                                                         <  1:1245755/100‑1 (MQ=255)                                            tCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatc                                                       <  1:911406/100‑1 (MQ=255)                                             cGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatca                                                      >  2:1678534/1‑100 (MQ=255)                                             cGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACtcatca                                                      >  2:1678515/1‑100 (MQ=255)                                              gTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAg                                                     <  2:318582/100‑1 (MQ=255)                                              gTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAg                                                     <  2:30002/100‑1 (MQ=255)                                               ttCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAgg                                                    >  1:1326228/1‑100 (MQ=255)                                                tCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGt                                                   >  1:299643/1‑100 (MQ=255)                                                  gCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTc                                                  <  2:519799/99‑1 (MQ=255)                                                   cTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTa                                                <  2:846354/100‑1 (MQ=255)                                                   cTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTa                                                <  2:400810/100‑1 (MQ=255)                                                      tCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTacac                                             >  1:1035957/1‑100 (MQ=255)                                                        gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACaa                                           >  1:336194/1‑100 (MQ=255)                                                        gATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGATCGAGTGTCAGCGTTGCGGCAAGCCGATTAATCCTAAAGTCTa                                                >  1:431995/1‑95 (MQ=255)                                                          tAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         <  1:1357934/100‑1 (MQ=255)                                                          tAACCAACGTCTCGCAGTGCTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACg                                         <  2:824134/100‑1 (MQ=255)                                                            aCCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTa                                       >  2:630535/1‑100 (MQ=255)                                                             ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  2:284556/1‑100 (MQ=255)                                                             ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  1:2258561/1‑100 (MQ=255)                                                             ccAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTAt                                      >  1:2274767/1‑100 (MQ=255)                                                                  gTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttt                                 <  1:1273744/100‑1 (MQ=255)                                                                   tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                >  1:296709/1‑100 (MQ=255)                                                                   tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGtttt                                <  1:2084273/100‑1 (MQ=255)                                                                   tctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGttgt                                >  2:1165164/1‑98 (MQ=255)                                                                    ctcGCAGTGTTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTa                               <  1:2013892/100‑1 (MQ=255)                                                                      cGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAg                              <  2:1324796/99‑1 (MQ=255)                                                                      cGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGt                             >  2:1134847/1‑100 (MQ=255)                                                                      cGCAGTGTAAAACGCCGATGCGCAGCTGACAGTCCAGCTCCAGTGTCAGCGTTGCGGGCAGCCGTTTACTCATCAGTTCTATACAACGTATTGtttt                                <  2:813978/97‑1 (MQ=255)                                                                       gcggtgtTAAACGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTc                            <  2:362067/97‑1 (MQ=255)                                                                            gtTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgtg                       <  2:2328181/100‑1 (MQ=255)                                                                            gtTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCtgtg                       <  2:1445009/100‑1 (MQ=255)                                                                            gtTAAACGGAGATGCGAAGGTGACAGTAACGATCGAGTGTAAGCGTTGCGGGAAGCAGTTTACTCATTAGGTATACACAAAGTATTGTTTTAGtt                            >  2:2166379/1‑94 (MQ=255)                                                                              tAAACGGCGATGCGAAGGTGACGGTAATGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     >  1:2252589/1‑100 (MQ=255)                                                                              tAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     >  1:502385/1‑100 (MQ=255)                                                                              tAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCg                     <  2:1272947/100‑1 (MQ=255)                                                                               aaaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGt                    >  2:2339732/1‑100 (MQ=255)                                                                               aaaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGt                    <  2:1031845/100‑1 (MQ=255)                                                                               aaaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTATACACAACGTATTGTTTTAGTCCTGTGCg                     >  2:1005155/1‑99 (MQ=255)                                                                                aaCGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGtt                   >  1:526164/1‑100 (MQ=255)                                                                                aaCGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGtt                   <  2:257531/100‑1 (MQ=255)                                                                                aaCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGtt                   <  2:398192/100‑1 (MQ=255)                                                                                 aCGGCGATGCGAAGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTc                  <  2:388501/100‑1 (MQ=255)                                                                                     cGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc              <  2:1798317/100‑1 (MQ=255)                                                                                     cGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc              >  2:1630744/1‑100 (MQ=255)                                                                                     cGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGAc              <  1:990139/100‑1 (MQ=255)                                                                                            aaGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAgg       <  1:1124529/100‑1 (MQ=255)                                                                                            aaGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAgg       <  2:1961538/100‑1 (MQ=255)                                                                                             aGGTGACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGc      <  1:1049503/100‑1 (MQ=255)                                                                                               gTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTg    >  2:1474660/1‑100 (MQ=255)                                                                                                 gACAGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGaa  >  1:2065280/1‑100 (MQ=255)                                                                                                    |                                                                                                 CGAATCCGTAGTCAGTGTGGACAGTGATGTGGAATGCTCCATGTCGTTCGCTATCGATAACCAACGTCTCGCAGTGTTAAACGGCGATGCGAAGGTGACGGTAACGCTCGAGTGTCAGCGTTGCGGGAAGCCGTTTACTCATCAGGTCTACACAACGTATTGTTTTAGTCCTGTGCGTTCAGACGAACAGGCTGAA  >  NC_000913/1146898‑1147093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑