Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0130G.95.4% 277.5 / 10.1 66intergenic (‑132/+56)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base . (34/28);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCG  >  NC_000913/379916‑380108
                                                                                                  |                                                                                               
ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                               >  2:891737/1‑100 (MQ=255)
ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                               >  1:1411919/1‑100 (MQ=255)
 gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                <  1:1971713/98‑1 (MQ=255)
       aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                                                                        <  1:1604116/100‑2 (MQ=255)
        ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                                                                       <  1:71498/100‑3 (MQ=255)
            atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATc                                                                                  >  1:579920/1‑100 (MQ=255)
             tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCg                                                                                 <  1:590434/100‑1 (MQ=255)
             tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCg                                                                                 >  1:983752/1‑100 (MQ=255)
              atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCg                                                                                 <  2:871401/100‑1 (MQ=255)
               tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGc                                                                               >  1:1706293/1‑100 (MQ=255)
                  cAATTCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAgg                                                                            <  2:870922/100‑1 (MQ=255)
                   aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAggg                                                                           <  1:1479068/100‑1 (MQ=255)
                    aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGa                                                                          >  1:753581/1‑100 (MQ=255)
                         tACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                     >  1:563625/1‑100 (MQ=255)
                          aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                    >  1:93488/1‑100 (MQ=255)
                          aCCCCCCTATAGTATATAACACTGGAGAATAAAATTTACCCGGTGAACGTGGGCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                     <  1:190319/99‑1 (MQ=255)
                           ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                   >  2:1541964/1‑100 (MQ=255)
                           ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                    >  1:692739/1‑99 (MQ=255)
                            cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                  >  1:187824/1‑100 (MQ=255)
                            cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                  >  2:1656803/1‑100 (MQ=255)
                            cccccTAAAGTATAAAAAAATGGGAGAAAAAAATTTTCCGGGTGAATTTGTTGGGAAAAAAAAGGGAAAGGGGGGGGGGCTAATCGGCAGGGAAgg                                                                       <  1:7094/96‑1 (MQ=255)
                             ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                 >  1:1177981/1‑100 (MQ=255)
                             ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                 >  2:2090679/1‑100 (MQ=255)
                             ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                 >  2:924848/1‑100 (MQ=255)
                                cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                              >  1:1595530/1‑100 (MQ=255)
                                cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                              >  1:1088581/1‑100 (MQ=255)
                                  ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                            <  2:1264716/100‑1 (MQ=255)
                                   taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                           >  1:1928699/1‑100 (MQ=255)
                                       atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                       >  1:999259/1‑100 (MQ=255)
                                          taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                    >  2:447233/1‑100 (MQ=255)
                                          taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                    <  2:753582/100‑1 (MQ=255)
                                            acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                  <  2:1771238/100‑1 (MQ=255)
                                            acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                 >  1:930524/1‑100 (MQ=255)
                                             cacTGGAGGATAATATTTACGCGTTGAATGTGGTCGGAAAACAAAGAGGAAAGTGGGGGGACTAATCGGCAAGGAAGGCCGCCCCGGATCgcgggcgg                                                   >  1:1093742/1‑98 (MQ=255)
                                               cTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                                >  1:1323032/1‑100 (MQ=255)
                                                tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                              <  1:1099029/100‑1 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                             >  2:398070/1‑100 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                             >  1:1058633/1‑100 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                             >  2:1548969/1‑100 (MQ=255)
                                                   gagaATAAAATTTATCCGGTTAATGTGGTCGGAAAATAAAGAGGAAAGGGGGGGAGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                            <  2:1177133/100‑1 (MQ=255)
                                                   gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                            <  2:2181969/100‑1 (MQ=255)
                                                      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                         <  1:762498/100‑1 (MQ=255)
                                                      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAAAGAAAAGGGGGGGGGCTACTGGGCAGGGAAGGACGCCCCGGATAGCGGGCGGCAGAAGGaag                                         >  1:912992/1‑99 (MQ=255)
                                                       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                        >  2:954960/1‑100 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                       >  2:498855/1‑100 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                       <  1:392612/100‑1 (MQ=255)
                                                           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                    <  1:1005748/100‑1 (MQ=255)
                                                            aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                   <  1:1092084/100‑1 (MQ=255)
                                                               tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                <  2:465576/100‑1 (MQ=255)
                                                                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                              <  2:2116970/100‑1 (MQ=255)
                                                                  ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                             >  1:1995975/1‑100 (MQ=255)
                                                                  ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                             >  2:1681444/1‑100 (MQ=255)
                                                                   cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                            <  2:1595539/100‑1 (MQ=255)
                                                                   cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                            <  2:2202847/100‑1 (MQ=255)
                                                                     gTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                          <  2:2175188/100‑1 (MQ=255)
                                                                         aTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                      <  1:1541957/100‑1 (MQ=255)
                                                                          tgtgGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                     >  1:934853/1‑100 (MQ=255)
                                                                           gtgGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                     <  2:2004075/99‑1 (MQ=255)
                                                                            tgGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                   <  2:1688446/100‑1 (MQ=255)
                                                                              gTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                 >  1:936349/1‑100 (MQ=255)
                                                                               tCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                <  2:1411925/100‑1 (MQ=255)
                                                                                     aaCAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt          <  2:106296/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg         >  1:1823718/1‑100 (MQ=255)
                                                                                      aCAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg         <  1:1408461/100‑1 (MQ=255)
                                                                                       cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc        <  2:1928709/100‑1 (MQ=255)
                                                                                       cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc        >  2:1085308/1‑100 (MQ=255)
                                                                                        aaaGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa       <  2:629122/100‑1 (MQ=255)
                                                                                        aaaGAGGAAAGGGGGGGAGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa       <  2:23950/100‑1 (MQ=255)
                                                                                             ggAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg  >  1:207941/1‑100 (MQ=255)
                                                                                             ggAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg  >  2:1697467/1‑100 (MQ=255)
                                                                                             ggAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg  >  1:115035/1‑100 (MQ=255)
                                                                                                  |                                                                                               
GGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCG  >  NC_000913/379916‑380108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: