Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,494,883 (A)7→8 coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,494,8761.A96.1% 360.3 / 6.0 104coding (254/564 nt)idnKD‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (3/1);  new base A (53/45);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494777‑4494970
                                                                                                    |                                                                                                  
tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAtt                                                                                                     >  1:585855/1‑100 (MQ=255)
tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAtt                                                                                                     <  1:61636/100‑1 (MQ=255)
   cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaa                                                                                               >  1:235635/1‑100 (MQ=255)
   cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaa                                                                                               >  1:1134037/1‑100 (MQ=255)
   cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaa                                                                                               >  2:871236/1‑100 (MQ=255)
     gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaa                                                                                            >  1:1981965/1‑100 (MQ=255)
     gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaa                                                                                            >  2:1833951/1‑100 (MQ=255)
         aaGATCGGCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                        <  2:1949315/100‑3 (MQ=255)
         aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                        <  2:299825/100‑3 (MQ=255)
         aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                        <  2:698594/100‑3 (MQ=255)
         aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATCAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                        <  1:1189694/100‑3 (MQ=255)
         aaGATCGACTTCCCTGGCTGGAACGCTGAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACATGATTTATTGTCTGTTCATCATT‑‑aaaaaaaac                                                                                        <  1:1081587/100‑3 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaaca                                                                                       >  1:816022/1‑97 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaaca                                                                                       >  1:1042262/1‑97 (MQ=255)
          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑aaaaaaaaca                                                                                       >  2:2056459/1‑97 (MQ=255)
              cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTa                                                                                    >  2:708530/1‑99 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGCTTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                  >  2:1583776/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                  >  2:2147378/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                  >  1:184840/1‑100 (MQ=255)
               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                  >  1:1779892/1‑100 (MQ=255)
                  ttCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                               >  1:1066317/1‑100 (MQ=255)
                  ttCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                               >  2:902601/1‑100 (MQ=255)
                  ttCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                               <  2:1167515/100‑1 (MQ=255)
                    cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGat                                                                             >  2:1982788/1‑100 (MQ=255)
                     ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTg                                                                               >  2:1453796/1‑97 (MQ=255)
                      ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGatat                                                                           <  2:146142/100‑1 (MQ=255)
                      ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGatan                                                                           <  2:2059259/100‑2 (MQ=255)
                       tggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATAtt                                                                          <  2:1376121/100‑1 (MQ=255)
                         gctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATAttt                                                                         <  1:266672/99‑1 (MQ=255)
                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTAc                                                                      <  2:1023506/100‑1 (MQ=255)
                            ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     >  1:233005/1‑100 (MQ=255)
                            ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     <  1:870770/100‑1 (MQ=255)
                              aaCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTAATAATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     <  1:949929/98‑1 (MQ=255)
                                cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 <  1:367379/100‑1 (MQ=255)
                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  2:2122762/1‑100 (MQ=255)
                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:1446704/1‑100 (MQ=255)
                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:467911/1‑100 (MQ=255)
                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                             >  2:1041261/1‑100 (MQ=255)
                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTa                                                             >  1:1047198/1‑100 (MQ=255)
                                     aaaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAg                                                            <  2:585856/100‑1 (MQ=255)
                                      aaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGc                                                           <  1:1266706/100‑1 (MQ=255)
                                       atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATAATAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                          <  1:1584531/100‑1 (MQ=255)
                                        tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                         >  2:1544533/1‑100 (MQ=255)
                                        tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATGTATTGTCTGTTCATCATT‑‑AGAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                         >  1:1213478/1‑100 (MQ=255)
                                         gatgCTTCATCAAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAAGCAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                        <  1:1462106/100‑1 (MQ=255)
                                          atgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccccc                                                       >  2:1815272/1‑100 (MQ=255)
                                            gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                     >  2:743833/1‑100 (MQ=255)
                                             cTTCATACAGTCTTTATAAAAAGAATGAACCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                    <  2:124581/100‑1 (MQ=255)
                                               tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                  <  2:636794/100‑1 (MQ=255)
                                                cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCACGGGTAGCCCCCAt                                                     >  1:184989/1‑96 (MQ=255)
                                                 aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                <  2:1461042/100‑1 (MQ=255)
                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                               >  1:1161624/1‑100 (MQ=255)
                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                               >  1:1448652/1‑100 (MQ=255)
                                                     aGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                            >  1:2082930/1‑100 (MQ=255)
                                                     aGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                            <  2:1112316/100‑1 (MQ=255)
                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                            >  1:1636310/1‑99 (MQ=255)
                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGCGATATGTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                           >  1:1503149/1‑100 (MQ=255)
                                                         tttATAAAAATAATGAAACAGGATTGATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  1:1245662/100‑1 (MQ=255)
                                                         tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTGAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  1:1424658/100‑1 (MQ=255)
                                                         tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        >  2:514718/1‑100 (MQ=255)
                                                         tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  2:1917382/100‑1 (MQ=255)
                                                         tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        <  1:833190/100‑1 (MQ=255)
                                                         tttATAAAAAGAAGGAAACAGTATTTATGGTTTTTTCATCATTAAAAAAAACATTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         <  1:648802/99‑1 (MQ=255)
                                                           tataAAAAGAATGAAACCGGCTTTATTGTCCGCTCATCATT‑‑AAACAAAACAGTATCGTGATACTTTACGCACGACTAGCCCCCATGTTCATTTCctctcg                                      >  1:434364/1‑98 (MQ=255)
                                                           tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctcttg                                      <  2:1496971/100‑3 (MQ=255)
                                                            ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     <  2:279504/100‑1 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAAACAGTGTCGTGATATTTTACGCTCGGGTAGCCCCCATGCTCATTTCGTCTGGt                                     >  1:571661/1‑100 (MQ=255)
                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGAAAGGGTACCCCCCATGTTCATGTCCTCTGCtt                                    >  1:2011098/1‑100 (MQ=255)
                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTCAAAAAAACAGTATCGCGATATTTTACGCCTGGGTATCACCCATGTTCGATTTCTCTGGTTa                                   >  1:587502/1‑100 (MQ=255)
                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                   >  2:1754886/1‑100 (MQ=255)
                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  <  2:1539152/100‑1 (MQ=255)
                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  2:2197975/1‑100 (MQ=255)
                                                                aaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                 <  2:562131/100‑1 (MQ=255)
                                                                aaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                 <  1:997203/100‑1 (MQ=255)
                                                                     aTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            <  1:2123891/100‑1 (MQ=255)
                                                                     aTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            >  1:677942/1‑100 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                          <  2:2089632/100‑1 (MQ=255)
                                                                       gAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAATGGTAGCCACCATGATCATATCATCTGGTTAGATGGCGACt                         >  1:1351065/1‑100 (MQ=255)
                                                                        aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                         >  2:518430/1‑100 (MQ=255)
                                                                        aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                         >  1:616262/1‑100 (MQ=255)
                                                                        aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                         >  2:866863/1‑100 (MQ=255)
                                                                          aCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                       <  2:184840/100‑1 (MQ=255)
                                                                             ggATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                    <  1:1508484/100‑1 (MQ=255)
                                                                               aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:937945/100‑1 (MQ=255)
                                                                               aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:938166/100‑1 (MQ=255)
                                                                               aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:1339863/100‑1 (MQ=255)
                                                                               aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  1:411326/100‑1 (MQ=255)
                                                                                      gTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc           >  1:1614540/1‑100 (MQ=255)
                                                                                       tCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg          >  2:2017801/1‑100 (MQ=255)
                                                                                        cTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgc         <  1:2093580/100‑1 (MQ=255)
                                                                                         tGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        <  1:45178/100‑1 (MQ=255)
                                                                                         tGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        >  1:249902/1‑100 (MQ=255)
                                                                                         tGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        >  1:248957/1‑100 (MQ=255)
                                                                                          gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc       >  2:1721492/1‑100 (MQ=255)
                                                                                          gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc       >  1:918689/1‑100 (MQ=255)
                                                                                          gTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc       >  1:1640562/1‑100 (MQ=255)
                                                                                           ttCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgcg      <  2:670793/100‑1 (MQ=255)
                                                                                            tcatcaTTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGa     <  2:1102756/100‑1 (MQ=255)
                                                                                              atcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAt   >  1:1098030/1‑100 (MQ=255)
                                                                                               tcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg  <  2:543336/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑‑AAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494777‑4494970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: