Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,489,197 C→T D322D (GAC→GAT)  aldA → aldehyde dehydrogenase A, NAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,489,1970CT98.7% 252.9 / ‑4.4 76D322D (GAC→GAT) aldAaldehyde dehydrogenase A, NAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (45/30);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCG  >  NC_000913/1489101‑1489287
                                                                                                |                                                                                          
tgtTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATAtt                                                                                         >  2:1247005/1‑100 (MQ=255)
  tttATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGc                                                                                       <  2:1588102/100‑1 (MQ=255)
        tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg                                                                                 >  1:1921424/1‑100 (MQ=255)
        tACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATggg                                                                                 >  2:2048030/1‑100 (MQ=255)
          cAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCGCGCTGAACGCAACGATATTGCGATGGGGc                                                                               >  1:387934/1‑100 (MQ=255)
          cAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGc                                                                               >  1:1042799/1‑100 (MQ=255)
            gAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCg                                                                             >  1:1137420/1‑100 (MQ=255)
              aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGtt                                                                           >  1:258330/1‑100 (MQ=255)
              aaGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGtt                                                                           >  1:606250/1‑100 (MQ=255)
                ggCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGa                                                                         >  2:1021636/1‑100 (MQ=255)
                 gCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAt                                                                        <  1:1733378/100‑1 (MQ=255)
                 gCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAt                                                                        <  2:1604535/100‑1 (MQ=255)
                  cATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGAtt                                                                       >  1:951017/1‑100 (MQ=255)
                   aTTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTa                                                                      <  2:1589166/100‑1 (MQ=255)
                   aTTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTa                                                                      >  1:1698549/1‑100 (MQ=255)
                    tttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTaa                                                                     >  2:899160/1‑100 (MQ=255)
                    tttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGAtt                                                                       >  2:1857433/1‑98 (MQ=255)
                     ttATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAc                                                                    <  1:1681624/100‑1 (MQ=255)
                      tATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcg                                                                   >  2:484832/1‑100 (MQ=255)
                       aTGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgc                                                                  >  2:960984/1‑100 (MQ=255)
                        tGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgcc                                                                 <  2:1118155/100‑1 (MQ=255)
                        attaaggTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgcc                                                                 >  1:1667487/7‑100 (MQ=255)
                         gATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAAcgccg                                                                >  2:1012366/1‑100 (MQ=255)
                           tCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCcgcg                                                              >  1:1200268/1‑100 (MQ=255)
                             aGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCgcggc                                                            >  2:1930907/1‑100 (MQ=255)
                                 cGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTg                                                        >  2:1022448/1‑100 (MQ=255)
                                 cGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATCGCGATGGGGCCGTTGATTAACGCCGCGGCGCTg                                                        <  1:1470479/100‑1 (MQ=255)
                                  gTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTgg                                                       <  2:1127655/100‑1 (MQ=255)
                                    cAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTg                                                        >  1:879118/1‑97 (MQ=255)
                                    cAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGaa                                                     >  2:1592345/1‑100 (MQ=255)
                                     aaTCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGaa                                                     >  2:321331/1‑99 (MQ=255)
                                     aaTCGGCGGGGTGAAGCGATGCAGGCGGTTCAAATTGGTAACCCCGCTGAACGCAACGATATTACAATGGGTCGCCTGATTAACGCCCATGCGCTGGaaa                                                    >  1:1110984/1‑100 (MQ=255)
                                         ggCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAggg                                                 >  2:605039/1‑99 (MQ=255)
                                         ggCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGt                                                >  1:1071060/1‑100 (MQ=255)
                                          gCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTc                                               <  2:1667496/100‑1 (MQ=255)
                                           cTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCg                                              <  2:1042800/100‑1 (MQ=255)
                                            tGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGa                                             >  1:1041874/1‑100 (MQ=255)
                                             gggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAg                                            >  1:1053230/1‑100 (MQ=255)
                                             gggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGACCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAg                                            <  2:270488/100‑1 (MQ=255)
                                              ggTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGc                                           <  1:205464/100‑1 (MQ=255)
                                               gTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCa                                          <  1:129167/100‑1 (MQ=255)
                                                  aaGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCaaaa                                       >  1:1345434/1‑100 (MQ=255)
                                                    gCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAg                                     >  2:950300/1‑100 (MQ=255)
                                                      gATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGGAAGGGTCGAGCAAAAAGTg                                   >  1:452427/1‑100 (MQ=255)
                                                      gATGCAGGCGGTTAAATTTGGTAACCCCGCTGAAGGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTg                                   <  1:490143/100‑1 (MQ=255)
                                                           aGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcg                              >  1:627869/1‑100 (MQ=255)
                                                            ggCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgc                             >  1:705557/1‑100 (MQ=255)
                                                            ggCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCAGCGGCGCTAGAAAGGGTCGAGCAAAAAGTGgcgcgc                             >  1:767470/1‑100 (MQ=255)
                                                             gCGGTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgcg                            <  1:1022447/100‑1 (MQ=255)
                                                              cGGTGCAATTTGGTAACCCCACTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGgcgcgcgc                           <  2:812552/100‑1 (MQ=255)
                                                               ggTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCa                          <  2:1857450/100‑1 (MQ=255)
                                                                gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg                         <  2:606251/100‑1 (MQ=255)
                                                                gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg                         >  1:430447/1‑100 (MQ=255)
                                                                gTTCAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAg                         >  2:1938738/1‑100 (MQ=255)
                                                                   cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag                      >  1:87335/1‑100 (MQ=255)
                                                                   cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag                      >  1:698190/1‑100 (MQ=255)
                                                                   cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag                      >  1:1065081/1‑100 (MQ=255)
                                                                   cAATTTGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTag                      >  1:2052098/1‑100 (MQ=255)
                                                                       ttGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTagaaga                  <  2:951018/100‑1 (MQ=255)
                                                                        tGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAgaagaa                 <  2:2049831/100‑1 (MQ=255)
                                                                        tGGTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAgaagaa                 >  2:920856/1‑100 (MQ=255)
                                                                          gTAACCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgg               <  2:843097/100‑1 (MQ=255)
                                                                            aaCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAgggg             <  2:1020670/100‑1 (MQ=255)
                                                                             aCCCCGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGc            >  2:374810/1‑100 (MQ=255)
                                                                              ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg           >  2:546994/1‑100 (MQ=255)
                                                                              ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg           >  1:850826/1‑100 (MQ=255)
                                                                              ccccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg           >  1:1709118/1‑100 (MQ=255)
                                                                              ccccGCTGAACGCAACGATATTGCGATGGGGCCGGTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCg           <  2:1709127/100‑1 (MQ=255)
                                                                                ccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgag         <  2:1053231/100‑1 (MQ=255)
                                                                                ccGCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCgag         <  1:1623997/100‑1 (MQ=255)
                                                                                  gCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGagag       <  2:2158004/100‑1 (MQ=255)
                                                                                  gCTGAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGagag       <  1:1040469/100‑1 (MQ=255)
                                                                                     gAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg    <  2:1059523/100‑1 (MQ=255)
                                                                                     gAACGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTgg    <  1:461276/100‑1 (MQ=255)
                                                                                       aCGCAACGATATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCg  <  1:1021635/100‑1 (MQ=255)
                                                                                        cGCAACGATATTGCGGTGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGGGGCg  >  1:1993123/1‑99 (MQ=255)
                                                                                                |                                                                                          
TGTTTATGTACAGAAAGGCATTTATGATCAGTTCGTCAATCGGCTGGGTGAAGCGATGCAGGCGGTTCAATTTGGTAACCCCGCTGAACGCAACGACATTGCGATGGGGCCGTTGATTAACGCCGCGGCGCTGGAAAGGGTCGAGCAAAAAGTGGCGCGCGCAGTAGAAGAAGGGGCGAGAGTGGCG  >  NC_000913/1489101‑1489287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑