| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,554,731 | C→T | M313I (ATG→ATA) | maeA ← | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,554,731 | 0 | C | T | 91.2% | 234.2 / 11.5 | 80 | M313I (ATG→ATA) | maeA | malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme |
| Reads supporting (aligned to +/- strand): ref base C (3/4); new base T (45/28); total (48/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTG > NC_000913/1554638‑1554828 | cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCg > 2:1050345/1‑100 (MQ=255)cTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCg > 2:1883098/1‑100 (MQ=255) tGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGc > 1:725477/1‑100 (MQ=255) tGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGc < 2:2102569/100‑1 (MQ=255) tGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGc > 2:2205456/1‑100 (MQ=255) gtcagtcaGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCgtc > 1:435099/1‑97 (MQ=255) gtcagtcaGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCa > 2:423140/1‑100 (MQ=255) gtcagtcaGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCa > 2:1989778/1‑100 (MQ=255) cagtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCaa > 2:595/1‑99 (MQ=255) agtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATg > 1:278940/1‑100 (MQ=255) gtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGc > 1:2045294/1‑100 (MQ=255) cagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCg < 2:2017727/100‑1 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa < 2:1993672/100‑1 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa > 2:393688/1‑100 (MQ=255) gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCa < 2:1645175/100‑1 (MQ=255) cAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCan < 1:1579718/100‑2 (MQ=255) aaGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATc < 1:1159566/100‑1 (MQ=255) gCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATccc > 2:780578/1‑100 (MQ=255) cAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGc > 1:1729970/1‑100 (MQ=255) cAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGc > 2:146183/1‑100 (MQ=255) aaaGCGATCTACCAGAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGc < 2:408596/99‑1 (MQ=255) gCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGaa < 1:2123713/100‑1 (MQ=255) cGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc > 2:1717971/1‑100 (MQ=255) cGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAc > 1:1017651/1‑100 (MQ=255) gATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAAcc > 2:664057/1‑100 (MQ=255) aTCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCt > 2:981412/1‑100 (MQ=255) tCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTg > 1:2052112/1‑100 (MQ=255) tCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTg < 2:561212/100‑1 (MQ=255) gACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcg > 2:1448537/1‑100 (MQ=255) aCCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTgcgc > 2:2078626/1‑100 (MQ=255) aaaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAgg > 1:143957/1‑100 (MQ=255) aaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGa < 1:1263583/100‑1 (MQ=255) aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGaa > 1:95299/1‑100 (MQ=255) gACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAg < 1:1392112/100‑1 (MQ=255) cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGAc < 2:797334/100‑1 (MQ=255) ttCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGa > 1:1841456/1‑100 (MQ=255) cTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtt < 1:1641543/100‑1 (MQ=255) cTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtt < 1:1975001/100‑1 (MQ=255) tGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttt > 1:1760865/1‑100 (MQ=255) tGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttt > 2:135709/1‑100 (MQ=255) gccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttt > 2:1780626/1‑100 (MQ=255) ccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttttt > 2:687281/1‑100 (MQ=255) ccgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttttt < 2:559849/100‑1 (MQ=255) cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt > 2:1022299/1‑100 (MQ=255) cgcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt > 2:1795614/1‑100 (MQ=255) cgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtc < 2:2045306/100‑1 (MQ=255) gccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt < 1:173438/97‑1 (MQ=255) ccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtctc > 2:211217/1‑100 (MQ=255) ccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTtctc > 1:1926067/1‑100 (MQ=255) cgcTTCCGCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCg < 2:1621671/100‑1 (MQ=255) gcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGc < 1:299610/100‑1 (MQ=255) cTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCt > 1:1128439/1‑100 (MQ=255) ttCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCtt < 2:620631/100‑1 (MQ=255) tCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTa < 1:270172/100‑1 (MQ=255) ccTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTACGGCAATGCCGCATCCCGCCGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTaa > 1:1217794/1‑100 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGCTGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc < 2:1775022/100‑1 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc > 1:356650/1‑100 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc > 1:1844945/1‑100 (MQ=255) cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAAc > 2:1593677/1‑100 (MQ=255) tCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACt < 1:2205442/100‑1 (MQ=255) gCTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGa > 2:291467/1‑100 (MQ=255) cTTAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGac < 2:1841466/100‑1 (MQ=255) tAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacca > 1:1441888/1‑100 (MQ=255) tAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacca > 1:2214168/1‑100 (MQ=255) tAATCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGacc > 1:1956063/1‑99 (MQ=255) aTCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccacc < 2:356651/100‑1 (MQ=255) aTCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccacc < 2:2042758/100‑1 (MQ=255) aTCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGaccacc < 2:1765322/100‑1 (MQ=255) tCCTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCt < 2:1128440/100‑1 (MQ=255) ccTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTg > 1:510532/1‑100 (MQ=255) ccTTCGCGCTGGGTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTg > 2:111065/1‑100 (MQ=255) gcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCcgcgcg < 2:1926077/100‑1 (MQ=255) gTCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtg > 2:600234/1‑100 (MQ=255) tCTGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgc < 1:291059/100‑1 (MQ=255) tGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctg < 2:483984/100‑1 (MQ=255) tGGGAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctg > 2:180486/1‑100 (MQ=255) gggAGATGATTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctgc > 1:1459/1‑100 (MQ=255) gatgatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGCCGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATc < 1:1062292/100‑1 (MQ=255) ngatTATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTCACTGACCACCTGCCGCGCGGCTTGCTGCGATCa > 1:2084362/2‑99 (MQ=255) atCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAgtg > 2:697203/1‑100 (MQ=255) | CTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTG > NC_000913/1554638‑1554828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |