| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,465,236 | C→T | P315P (CCG→CCA) | treB ← | trehalose‑specific PTS enzyme: IIB and IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,465,236 | 0 | C | T | 95.2% | 263.7 / 0.8 | 84 | P315P (CCG→CCA) | treB | trehalose‑specific PTS enzyme: IIB and IIC component |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (50/30); total (51/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATC > NC_000913/4465139‑4465329 | aCAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgg < 2:888242/100‑1 (MQ=255) cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTggg > 2:1018245/1‑100 (MQ=255) aGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgggt < 1:93339/100‑2 (MQ=255) aGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgggg < 2:246351/100‑1 (MQ=255) gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGc > 1:204255/1‑100 (MQ=255) cAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTa > 1:1990600/1‑100 (MQ=255) aaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTAc < 1:206388/100‑1 (MQ=255) aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACa > 1:1368935/1‑100 (MQ=255) aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACa > 1:1442250/1‑100 (MQ=255) tCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAg < 2:431342/100‑1 (MQ=255) cGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAg > 1:1736743/1‑100 (MQ=255) ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGc < 2:1631945/100‑1 (MQ=255) cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc > 1:2008770/1‑100 (MQ=255) cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAAc > 2:1143137/1‑100 (MQ=255) cTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACaa > 1:294360/1‑100 (MQ=255) cGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgct > 1:79317/1‑100 (MQ=255) cGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgct < 2:938696/100‑1 (MQ=255) tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc > 1:541221/1‑100 (MQ=255) accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcg < 2:1529098/100‑1 (MQ=255) accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTgcg < 2:549985/100‑1 (MQ=255) caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGcc < 2:479054/100‑1 (MQ=255) caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGcc < 2:1107483/100‑1 (MQ=255) accCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCa > 2:343415/1‑100 (MQ=255) accCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCa > 2:45264/1‑100 (MQ=255) accCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCa > 1:236080/1‑100 (MQ=255) cccATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCaa > 1:1002242/1‑100 (MQ=255) cccATGCTTTGAATCATCTGCAAATCAATAGCAAGCGCGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCaa > 1:1186428/1‑100 (MQ=255) tGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACGCCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg > 2:317808/1‑100 (MQ=255) tGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg < 2:2082612/100‑1 (MQ=255) cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg < 1:1859190/100‑1 (MQ=255) tttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGc < 1:1894627/100‑1 (MQ=255) gAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCaaa < 1:436044/100‑1 (MQ=255) aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg > 1:534684/1‑100 (MQ=255) atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGTCAATCGGAGCAAAGc > 2:2195899/1‑100 (MQ=255) atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc > 2:605932/1‑100 (MQ=255) atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc < 1:1507848/100‑1 (MQ=255) atcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGc > 2:362975/1‑100 (MQ=255) cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg > 1:1095524/1‑100 (MQ=255) tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg < 2:2212684/100‑1 (MQ=255) gCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGAGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGc > 1:2062348/1‑96 (MQ=255) cAAATCAATAGCGAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtc > 2:1459391/1‑100 (MQ=255) cAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtc > 2:984838/1‑100 (MQ=255) cAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtc > 1:560875/1‑100 (MQ=255) aaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcat < 2:1186430/100‑1 (MQ=255) aTCAATAGCAAGCGTGGTCTGGTGTAAACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc > 2:1354864/1‑100 (MQ=255) cAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAg > 1:370189/1‑100 (MQ=255) aaTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAgg < 2:970848/100‑1 (MQ=255) aTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGTCGTACCGGAAGACAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCACGt < 1:2117919/100‑1 (MQ=255) gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc > 2:349780/1‑100 (MQ=255) cAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACg > 1:488100/1‑100 (MQ=255) aGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGCGACGTa < 2:1551780/100‑1 (MQ=255) cGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc < 1:1760668/100‑1 (MQ=255) cGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc < 2:654098/100‑1 (MQ=255) tGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGc > 1:510375/1‑100 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa > 2:21024/1‑100 (MQ=255) gTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa > 2:1936833/1‑100 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg < 1:287858/100‑1 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg > 1:1972530/1‑100 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg > 1:1692443/1‑100 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg < 2:714746/100‑1 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg < 2:1990610/100‑1 (MQ=255) cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTCACGTACCGCaagg < 2:1508790/100‑3 (MQ=255) tGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAgg < 2:1285806/100‑1 (MQ=255) nggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGGCGTACCGCAAAgg > 1:1897307/2‑100 (MQ=255) ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc < 2:560876/100‑1 (MQ=255) ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc < 1:371612/100‑1 (MQ=255) ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc > 2:1584424/1‑100 (MQ=255) tgtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCaa < 2:660648/100‑1 (MQ=255) gtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc > 2:1830154/1‑100 (MQ=255) tACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACg > 1:1600648/1‑100 (MQ=255) ncacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc > 1:2202806/2‑100 (MQ=255) acacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc > 2:249252/1‑100 (MQ=255) cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc > 1:1540036/1‑100 (MQ=255) acCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCa > 1:1597740/1‑100 (MQ=255) ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt > 2:1925993/1‑100 (MQ=255) ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt > 2:1259568/1‑100 (MQ=255) gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGGCGTACCGCAAAGGCAACGCCATCGc < 2:1897317/100‑1 (MQ=255) gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc > 2:1431857/1‑100 (MQ=255) aTCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCaa > 2:1272945/1‑100 (MQ=255) cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc < 1:1584415/100‑1 (MQ=255) cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc > 2:1690008/1‑100 (MQ=255) cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc > 1:1498984/1‑100 (MQ=255) ccAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGACATAAGGTGACGTACGGCAAAGGCAACGCCATCGcgactc > 1:538994/1‑93 (MQ=255) ccAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCCGTAATCAGGCGACGTACCGCAACGGCAACGCCATCGCCaacc > 1:1791721/1‑96 (MQ=255) | ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATC > NC_000913/4465139‑4465329 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |