Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	410,479	A→G	S112S (TCA→TCG)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,479	0	A	G	100.0%	287.3 / NA	78	S112S (TCA→TCG)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/40);  total (38/40)

AACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410383‑410574                                                                                                 |                                                                                                aaCCTGGCTCAACGGTTAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGaa                                                                                              <  1:1300631/100‑1 (MQ=255)  aCCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAg                                                                                             <  2:589109/100‑1 (MQ=255)   ccTGGCTCAACGGTCAGCCATTCGATCAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:2039469/100‑1 (MQ=255)   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  2:1035578/100‑1 (MQ=255)   ccTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGc                                                                                            <  1:189444/100‑1 (MQ=255)      ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAg                                                                                          <  2:2188156/99‑1 (MQ=255)      ggCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGt                                                                                         >  1:1352728/1‑100 (MQ=255)       gCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTa                                                                                        <  2:251388/100‑1 (MQ=255)        cTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAg                                                                                       >  1:1702346/1‑100 (MQ=255)          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     >  2:1382606/1‑100 (MQ=255)          cAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGAt                                                                                     <  2:1937906/100‑1 (MQ=255)                 cAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCgg                                                                              <  1:1733526/100‑1 (MQ=255)                 cAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGCGAAGTGCGGCTGGCAAATGACGCTAACTGTATGGCGGTGTCGGAAGCAGTAGATgg                                                                                   >  1:1743885/1‑95 (MQ=255)                 cAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCCGGAAGTGCGGCTGGCAAATGACGCTGACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCgg                                                                              >  2:79886/1‑100 (MQ=255)                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGc                                                                             >  1:415403/1‑99 (MQ=255)                   gCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCa                                                                            >  2:529777/1‑100 (MQ=255)                     cATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGc                                                                          >  2:1481104/1‑100 (MQ=255)                      aTTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCg                                                                         >  2:1654539/1‑100 (MQ=255)                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCggg                                                                       >  1:422852/1‑99 (MQ=255)                         cGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGATTCGGAAGCAGTAGATGGCGCGGCAGCgg                                                                        >  1:2106261/1‑98 (MQ=255)                          gATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAg                                                                     >  1:199826/1‑100 (MQ=255)                           aTAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgc                                                                    <  2:1466835/100‑1 (MQ=255)                            tAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAgcg                                                                   <  2:1294391/100‑1 (MQ=255)                             aaaCACATAAGCGCGAGGTTGCAGCGGGAAGGGCGCCTGGCAAATGACGCTAACTGTCTGGCTGTTTCGGAAGCAGTAGATGGCGCGGCAGCGTGAgcgc                                                                  <  1:2010984/100‑1 (MQ=255)                              aaGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCa                                                                 >  1:1982272/1‑100 (MQ=255)                               aGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAg                                                                <  1:278370/100‑1 (MQ=255)                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                           >  2:1593333/1‑100 (MQ=255)                                    tAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGt                                                           >  2:27525/1‑100 (MQ=255)                                      aGCTCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAt                                                         <  2:183089/100‑1 (MQ=255)                                       gcgcGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTAtt                                                        >  1:2216766/1‑100 (MQ=255)                                         gcgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTg                                                      <  2:283257/100‑1 (MQ=255)                                          cgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGc                                                     <  2:568996/100‑1 (MQ=255)                                          cgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGc                                                     >  1:1927561/1‑100 (MQ=255)                                          cgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGc                                                     >  1:1059847/1‑100 (MQ=255)                                          cgAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGc                                                     <  2:1214706/100‑1 (MQ=255)                                            aGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCg                                                   >  1:2052616/1‑100 (MQ=255)                                             ggTTGCAGCGGGAAGTGCGGCTGGCATATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  1:1319321/100‑1 (MQ=255)                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  2:157818/100‑1 (MQ=255)                                             ggTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGt                                                  <  2:452760/100‑1 (MQ=255)                                              gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                 <  1:529776/100‑1 (MQ=255)                                              gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                 >  2:1005769/1‑100 (MQ=255)                                              gTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTg                                                 <  1:27924/100‑1 (MQ=255)                                                 gCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGAtt                                              >  2:1473348/1‑100 (MQ=255)                                                  cAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTa                                             >  2:1109343/1‑100 (MQ=255)                                                   aGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTAt                                            >  2:482339/1‑100 (MQ=255)                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGATGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTa                                             <  1:416992/98‑1 (MQ=255)                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATc                                           >  2:239254/1‑100 (MQ=255)                                                    gCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATc                                           >  1:687094/1‑100 (MQ=255)                                                       ggAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGc                                        >  1:259454/1‑100 (MQ=255)                                                          aGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACg                                     <  1:1667814/100‑1 (MQ=255)                                                            tGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGAGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACggg                                   <  1:1419289/100‑1 (MQ=255)                                                             gCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGa                                  >  1:1026294/1‑100 (MQ=255)                                                              cGGCTGGCAGATGGCGCTAACTGTCTGGCGGGTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                 <  1:308321/100‑1 (MQ=255)                                                              cGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAt                                 >  2:1182949/1‑100 (MQ=255)                                                                gCTGGCAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATgc                               <  2:40367/100‑1 (MQ=255)                                                                     cAAATGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGgcgc                          >  2:498904/1‑100 (MQ=255)                                                                      aaaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGcgcg                         <  2:1129075/100‑1 (MQ=255)                                                                      aaaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGcgcg                         <  2:259454/100‑1 (MQ=255)                                                                       aaTGACGCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCgg                        >  1:281244/1‑100 (MQ=255)                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                  <  2:1260926/100‑1 (MQ=255)                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                  <  1:1382604/100‑1 (MQ=255)                                                                             gCTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTgg                  <  1:1005768/100‑1 (MQ=255)                                                                              cTAACTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGc                 <  2:422853/100‑1 (MQ=255)                                                                                aaCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAt               >  1:910819/1‑100 (MQ=255)                                                                                aaCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAt               <  1:1931780/100‑1 (MQ=255)                                                                                 aCTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCAtt              <  2:1026295/100‑1 (MQ=255)                                                                                  cTGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTc             >  1:1769206/1‑100 (MQ=255)                                                                                   tGTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCa            >  1:725332/1‑100 (MQ=255)                                                                                    gTCTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCaa           >  2:26325/1‑100 (MQ=255)                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGGGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg         >  2:1360552/1‑100 (MQ=255)                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg         <  2:1927571/100‑1 (MQ=255)                                                                                      cTGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATg         >  1:454972/1‑100 (MQ=255)                                                                                       tGGCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgg        <  1:302069/100‑1 (MQ=255)                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg       <  1:972252/100‑1 (MQ=255)                                                                                        ggCGGTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATggg       <  2:987138/100‑1 (MQ=255)                                                                                          cGGTTTCGGAAGCAGTAGATGGCGCGGCAGGGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGTGTGGCACTCAATgggcg     >  1:1464958/1‑100 (MQ=255)                                                                                           ggTTTCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgg    >  2:791683/1‑100 (MQ=255)                                                                                             tttCGGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATgggcgggc  <  2:1982282/100‑1 (MQ=255)                                                                                                 |                                                                                                AACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGC  >  NC_000913/410383‑410574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑