Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,809,774:1 (T)7→8 100% coding (158/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,809,7671.T99.0% 359.2 / ‑3.3 103coding (151/738 nt)ygaZputative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (51/51);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 124 total aligned reads displayed.

GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC  >  NC_000913/2809669‑2809861
                                                                                                   |                                                                                               
gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGt                                                                                                >  2:1578097/1‑100 (MQ=255)
 aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtt                                                                                               >  2:1820113/1‑100 (MQ=255)
  tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttt                                                                                             >  1:2514822/1‑100 (MQ=255)
  tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttt                                                                                             >  1:1897111/1‑100 (MQ=255)
     aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGtttttt                                                                                          <  2:704748/100‑1 (MQ=255)
      aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttt                                                                                         >  2:35473/1‑100 (MQ=255)
      aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttt                                                                                         >  1:57129/1‑100 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGGTTCTCTCCTCTCGAAAGCGttttttttc                                                                                       <  1:2388993/100‑3 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                       <  2:2751991/100‑3 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                       <  2:2160194/100‑3 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                       <  1:2180961/100‑3 (MQ=255)
        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttc                                                                                       <  2:2317353/100‑3 (MQ=255)
         aCAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttct                                                                                      >  1:1089627/1‑97 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCTACCCGTCTGGGATTCTCTCCTCTCGGAAGCGttttttttctcc                                                                                    <  1:1497266/99‑6 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCt                                                                                    <  2:2289155/100‑1 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttctttt                                                                                   >  1:2020677/1‑94 (MQ=255)
            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttctc                                                                                     >  1:2370341/1‑94 (MQ=255)
             ttttCCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   <  2:2262144/100‑1 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCTTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   <  2:695544/100‑1 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   >  2:2245750/1‑100 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   >  1:406580/1‑100 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   >  1:149361/1‑100 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTg                                                                                   <  1:1578089/100‑1 (MQ=255)
             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGttttttttcgcctg                                                                                  >  2:2259393/1‑93 (MQ=255)
               tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGca                                                                                 >  2:2387224/1‑100 (MQ=255)
                 ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatc                                                                               >  2:325963/1‑100 (MQ=255)
                 ccGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcat                                                                                >  1:2380869/1‑99 (MQ=255)
                  cGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGcatca                                                                              >  2:2785090/1‑100 (MQ=255)
                     ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCAttt                                                                           >  1:1358661/1‑100 (MQ=255)
                     ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCAttt                                                                           <  2:2539317/100‑1 (MQ=255)
                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTAt                                                                         >  2:2608391/1‑100 (MQ=255)
                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTAt                                                                         >  2:107402/1‑100 (MQ=255)
                        ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATg                                                                        <  1:2970576/100‑1 (MQ=255)
                        ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATg                                                                        <  2:100618/100‑1 (MQ=255)
                        ttattaGTTATATTCAGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATg                                                                        <  2:3007022/100‑1 (MQ=255)
                         tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGc                                                                       <  1:654033/100‑1 (MQ=255)
                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCa                                                                      <  1:51039/100‑1 (MQ=255)
                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCa                                                                      >  1:2351425/1‑100 (MQ=255)
                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCa                                                                      <  2:2512115/100‑1 (MQ=255)
                            taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAgg                                                                    >  1:585762/1‑100 (MQ=255)
                             aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAgga                                                                   <  2:1806719/100‑2 (MQ=255)
                             aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCGCCTGCATAAATGATGCAgga                                                                   <  1:2259380/100‑2 (MQ=255)
                              gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGgcg                                                                  <  1:659971/100‑1 (MQ=255)
                               ttATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggaga                                                                 <  1:1160547/100‑4 (MQ=255)
                                tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGcgcg                                                                >  1:2186811/1‑100 (MQ=255)
                                 atatTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATTTTTTATGCATGCGCGa                                                               >  1:854670/1‑100 (MQ=255)
                                   atTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATAAATGATGCAggagaaaaa                                                             <  2:519430/100‑8 (MQ=37)
                                    ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                            <  1:899892/100‑1 (MQ=255)
                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                          >  2:2141893/1‑100 (MQ=255)
                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                         <  2:694806/100‑1 (MQ=255)
                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                         >  1:98175/1‑100 (MQ=255)
                                            gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                    <  2:570532/100‑1 (MQ=255)
                                            gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                    <  2:2437191/100‑1 (MQ=255)
                                              cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                  >  2:418462/1‑100 (MQ=255)
                                               tttGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAtt                                                 >  2:2890963/1‑100 (MQ=255)
                                                 tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                               >  2:606224/1‑100 (MQ=255)
                                                 tGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAc                                               <  2:2514836/100‑1 (MQ=255)
                                                   cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                             >  1:2412524/1‑100 (MQ=255)
                                                    gTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcgc                                            >  1:1260310/1‑100 (MQ=255)
                                                          tCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCt                                      <  2:1706338/100‑1 (MQ=255)
                                                           cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTTTTCATTTATGCAGGCGCGAGCCAGTTGGTCATTACCTTGATgtt                                      >  1:2028527/1‑97 (MQ=255)
                                                            tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                    >  1:820968/1‑100 (MQ=255)
                                                            tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                    <  2:850150/100‑1 (MQ=255)
                                                             gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   >  1:609402/1‑100 (MQ=255)
                                                             gAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGc                                   <  2:1012092/100‑1 (MQ=255)
                                                              aaTGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCa                                  <  2:3030034/100‑1 (MQ=255)
                                                                tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGc                                >  2:1412413/1‑100 (MQ=255)
                                                                tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGc                                >  2:1978854/1‑100 (MQ=255)
                                                                tGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGc                                >  2:2854514/1‑100 (MQ=255)
                                                                  cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTGATTACCGCGATGCTGGCAGCCg                              >  2:868486/1‑100 (MQ=255)
                                                                  cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCg                              >  2:868982/1‑100 (MQ=255)
                                                                  cGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCg                              >  2:102525/1‑100 (MQ=255)
                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                             >  2:606522/1‑100 (MQ=255)
                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                             >  2:122638/1‑100 (MQ=255)
                                                                    aCCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                            >  2:1560276/1‑100 (MQ=255)
                                                                      ccGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                          <  2:1089633/100‑1 (MQ=255)
                                                                       cGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                          >  2:2653368/1‑99 (MQ=255)
                                                                            gggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                    <  2:396866/100‑1 (MQ=255)
                                                                             ggATTCTTGCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtg                   <  1:971937/100‑1 (MQ=255)
                                                                             ggATTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCATGCGCGAGCCAGTTCGTCATTATCGCGATGCTGGCATCCGGGAGTAGTTtg                   >  1:2887133/1‑100 (MQ=255)
                                                                               aTTCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                 <  2:2025119/100‑1 (MQ=255)
                                                                                ttCTCTCCTCTCGAGGGCGTTGTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTgg                <  1:574941/100‑1 (MQ=255)
                                                                                ttCTCTCCTCTCGAAAGCGTTTTTTTTTTTTTTTTTCATTTATGCAGGCGCCAGCCCGTTCGTCCTTACCGCGATGCTGGCAGCCGGGAGTTTTTtgtt                 >  1:993138/1‑98 (MQ=255)
                                                                                ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                 <  2:2551079/99‑1 (MQ=255)
                                                                                ttCTCTCCTCTCGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                 <  1:327254/99‑1 (MQ=255)
                                                                                   tctcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAtt             <  1:2772098/100‑1 (MQ=255)
                                                                                     tcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc           <  1:1254404/100‑1 (MQ=255)
                                                                                     tcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc           <  2:2617131/100‑1 (MQ=255)
                                                                                     tcctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc           <  2:2012718/100‑1 (MQ=255)
                                                                                      cctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtact          >  1:839220/1‑100 (MQ=255)
                                                                                       ctctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg         <  2:1404774/100‑1 (MQ=255)
                                                                                        tctcGAAAGCGTTTTTTTTTTTTTTTTTTATTTATGCAGGCGCGAGCCATTTCGTCATTTCCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATttctgc        >  1:1639971/1‑100 (MQ=255)
                                                                                        tctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc        >  1:646181/1‑100 (MQ=255)
                                                                                        tctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc        <  2:868574/100‑1 (MQ=255)
                                                                                         ctcGAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa       >  1:1898873/1‑100 (MQ=255)
                                                                                            gAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg    <  1:304082/100‑1 (MQ=255)
                                                                                            gAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg    <  2:646186/100‑1 (MQ=255)
                                                                                            gAAAGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg    <  2:2412537/100‑1 (MQ=255)
                                                                                              aaGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg    >  1:216197/1‑98 (MQ=255)
                                                                                              aaGCGTTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAc  <  1:35472/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGCGTTTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGAC  >  NC_000913/2809669‑2809861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: