Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,494,883:1 (A)7→8 100% coding (261/564 nt) idnK → D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,494,8761.A95.5% 463.3 / 13.0 132coding (254/564 nt)idnKD‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base A (66/60);  total (69/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 154 total aligned reads displayed.

GATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494782‑4494970
                                                                                                |                                                                                                
gatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaa                                                                                           >  1:3046675/1‑100 (MQ=255)
 atgaAGATCGACTTCCCTGGCTGGAACGCTTAAATTATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaa                                                                                          <  2:2502113/100‑1 (MQ=255)
 atgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaa                                                                                          >  1:2086747/1‑100 (MQ=255)
  tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAAGAGGATTTACTGTCTGTTCATCATTaaaaaaa                                                                                         >  1:2059784/1‑100 (MQ=255)
    aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaac                                                                                       <  2:2649775/100‑3 (MQ=255)
    aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaac                                                                                       <  2:1977564/100‑3 (MQ=255)
     aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaaca                                                                                      >  2:554127/1‑97 (MQ=255)
     aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaac                                                                                       >  2:796811/1‑97 (MQ=255)
      gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaacag                                                                                     >  2:1149424/1‑96 (MQ=255)
      gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaacag                                                                                     >  2:506124/1‑96 (MQ=255)
       aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTaaaaaaaacagt                                                                                    >  2:2631956/1‑95 (MQ=255)
         cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTAt                                                                                   <  2:2426204/100‑1 (MQ=255)
         cGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTAt                                                                                   >  2:394035/1‑100 (MQ=255)
          gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATc                                                                                  >  1:1466468/1‑100 (MQ=255)
             tttccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAACAGTATCGTg                                                                               <  1:1183081/96‑1 (MQ=255)
               cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGat                                                                             <  2:2205838/100‑1 (MQ=255)
                ccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGata                                                                            >  1:900426/1‑100 (MQ=255)
                 ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGatat                                                                           >  1:356414/1‑100 (MQ=255)
                 ctggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGatat                                                                           <  1:1462462/100‑1 (MQ=255)
                  tggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATAtt                                                                          <  1:440250/100‑1 (MQ=255)
                   ggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATAttt                                                                         <  2:101757/100‑1 (MQ=255)
                    gctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATAtttt                                                                        <  1:7232/100‑1 (MQ=255)
                      tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTAc                                                                      <  1:2455077/100‑1 (MQ=255)
                       ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACg                                                                     <  2:1493020/100‑1 (MQ=255)
                        gAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGc                                                                    <  2:3012218/100‑1 (MQ=255)
                         aaCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAACAGTATCGTGATATTTTACGCaa                                                                  <  2:1505114/100‑1 (MQ=255)
                           cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 <  2:233224/100‑1 (MQ=255)
                           cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 <  2:2271742/100‑1 (MQ=255)
                            ncTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTCCGCa                                                                   >  1:2707186/2‑97 (MQ=255)
                            gCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAgg                                                                <  2:2652549/100‑1 (MQ=255)
                             cTTAAATGATGCTTCATCCAGTCTTTATAAAAAGAATGAAACCGGCTATATTGTCTGTTAATCATAAAAAAAAAAAGTATCATGATATGTTAc                                                                      >  1:1922591/1‑93 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTTTTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:2329969/1‑100 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               >  1:2625260/1‑100 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 >  1:1194735/1‑98 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAg                                                                 <  1:2936798/98‑1 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTAATAATTAAAAAAAACAGTATCGTGATATTTTACGCAAggg                                                               <  1:916384/100‑1 (MQ=255)
                             cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGCTCATCATTAAAAAAAACAGTATCGTGATATTTTAc                                                                      >  1:876610/1‑93 (MQ=255)
                               tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTAATAATTAAAAAAAACAGTATCATGATATTTTACGCAAGGGTa                                                             >  1:542897/1‑100 (MQ=255)
                                aaaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAg                                                            >  2:1797563/1‑100 (MQ=255)
                                  atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                          <  2:2612311/100‑1 (MQ=255)
                                  atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                          <  1:1280673/100‑1 (MQ=255)
                                  atgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGcc                                                          <  2:567152/100‑1 (MQ=255)
                                   tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                         <  2:2470418/100‑1 (MQ=255)
                                   tgatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGAAAAAAATAAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                         <  1:1073213/100‑1 (MQ=255)
                                      tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                      <  1:1398336/100‑1 (MQ=255)
                                       gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                      >  2:1342479/1‑99 (MQ=255)
                                       gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                     >  1:983516/1‑100 (MQ=255)
                                         ttCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGt                                                   >  1:783554/1‑100 (MQ=255)
                                           cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                 <  2:900431/100‑1 (MQ=255)
                                           cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                 >  1:897624/1‑100 (MQ=255)
                                            aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                  >  1:3038735/1‑98 (MQ=255)
                                            aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                <  1:3004862/100‑1 (MQ=255)
                                             tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                               <  1:2999957/100‑1 (MQ=255)
                                              aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                              <  2:2053211/100‑1 (MQ=255)
                                               cAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAttt                                             >  2:116286/1‑100 (MQ=255)
                                               cAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCACCAATGTTCAttt                                             >  1:780023/1‑100 (MQ=255)
                                                 gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                           >  2:1290431/1‑100 (MQ=255)
                                                 gTCTTTATAAAAAAAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                           >  2:2052962/1‑100 (MQ=255)
                                                   cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTCTACGCAAGGATACCCCCCATGTTcctgttc                                           >  1:1228349/1‑92 (MQ=255)
                                                   cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAACAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCct                                          >  2:1332187/1‑99 (MQ=255)
                                                     ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                       >  2:2870953/1‑100 (MQ=255)
                                                     ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                       <  1:696862/100‑1 (MQ=255)
                                                      tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  2:1552874/100‑1 (MQ=255)
                                                      tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  2:1764816/100‑1 (MQ=255)
                                                      tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGAGTAGCCCCCATGTTCATTTCCTCTgg                                      >  1:790143/1‑100 (MQ=255)
                                                       ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     <  2:2632395/100‑1 (MQ=255)
                                                       ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGt                                     >  2:2450675/1‑100 (MQ=255)
                                                        taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCTTGATATTTTACTCAAGGGTAACACCCATGTTCATTTCCTCTGGtt                                    >  1:2240588/1‑100 (MQ=255)
                                                        taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    >  1:2867084/1‑100 (MQ=255)
                                                        taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    >  1:1721004/1‑100 (MQ=255)
                                                        taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    >  1:866448/1‑100 (MQ=255)
                                                          aaaaGAATGAAACAGGTTTTTTTGTCTGTTCTTCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  1:1877186/1‑100 (MQ=255)
                                                          aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  <  1:2117493/100‑1 (MQ=255)
                                                          aaaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  2:2399966/1‑100 (MQ=255)
                                                           aaaGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                 <  2:2590734/100‑1 (MQ=255)
                                                             aGAATGAAACAGTATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                               <  1:2429734/100‑1 (MQ=255)
                                                             aGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                               >  2:713540/1‑100 (MQ=255)
                                                                aTGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            <  1:2277478/100‑1 (MQ=255)
                                                                  gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                          <  2:2958783/100‑1 (MQ=255)
                                                                  gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                          >  1:2509385/1‑100 (MQ=255)
                                                                  gAAACAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGAc                          >  2:2348694/1‑100 (MQ=255)
                                                                   aaaCAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                         >  1:2251302/1‑100 (MQ=255)
                                                                      cAGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATg                      >  1:484162/1‑100 (MQ=255)
                                                                       aGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                     <  1:1114736/100‑1 (MQ=255)
                                                                       aGGATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                     >  2:3052294/1‑100 (MQ=255)
                                                                        ggATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                    >  2:1702486/1‑100 (MQ=255)
                                                                        ggATTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                    <  1:1316150/100‑1 (MQ=255)
                                                                         gATTTATTGTGTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaaa                   <  1:2014179/100‑1 (MQ=255)
                                                                          aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:1660928/100‑1 (MQ=255)
                                                                          aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  2:443510/100‑1 (MQ=255)
                                                                          aTTTATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  1:1273990/100‑1 (MQ=255)
                                                                            ttATTGTATGTTCATCATTAAACAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                  <  1:2594098/98‑1 (MQ=255)
                                                                             tATTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt               <  2:1272835/100‑1 (MQ=255)
                                                                              aTTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAtt              >  1:265779/1‑100 (MQ=255)
                                                                              aTTGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAtt              >  2:70448/1‑100 (MQ=255)
                                                                               ttGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtc             <  2:1877197/100‑1 (MQ=255)
                                                                                tGTCTGTTCATCATTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct            <  2:396179/100‑1 (MQ=255)
                                                                                        catcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGaa    >  1:1169332/1‑100 (MQ=255)
                                                                                         atcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAAt   <  2:2871436/100‑1 (MQ=255)
                                                                                          tcatTAAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg  >  2:809699/1‑100 (MQ=255)
                                                                                                |                                                                                                
GATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494782‑4494970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: