| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,645,886 | (C)5→4 | coding (1440/2313 nt) | pbpC ← | penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,645,882 | 0 | C | . | 98.6% | 315.6 / ‑2.1 | 69 | coding (1444/2313 nt) | pbpC | penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (36/32); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG > NC_000913/2645784‑2645977 | ggTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACgccgcc > 2:484018/1‑100 (MQ=255)ggTGCCCGTTTTCCATGCCAGCGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACgccgcc < 1:2086417/100‑1 (MQ=255) gTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccc > 1:2781631/1‑100 (MQ=255) tGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGcccc > 1:717524/1‑100 (MQ=255) cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga > 1:265659/1‑98 (MQ=255) cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga < 2:1783856/100‑3 (MQ=255) cccGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGccccga > 1:2475241/1‑98 (MQ=255) ttttCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCgacgac < 2:1179805/100‑1 (MQ=255) ttttCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATCATCCACGCCG‑CCCCgacgac < 2:1823978/100‑1 (MQ=255) ttCCATGCCCGTGGGGCGAGGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCGTCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACAt < 2:1387489/100‑1 (MQ=255) ttCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACAt < 2:2167043/100‑1 (MQ=255) tCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACAtt > 1:912120/1‑100 (MQ=255) ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTa > 2:2559172/1‑100 (MQ=255) ccATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTa > 2:1587565/1‑100 (MQ=255) cATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTaa < 2:313792/100‑1 (MQ=255) aTGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTaaa < 1:552107/100‑1 (MQ=255) aTGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTaaa > 2:2382480/1‑100 (MQ=255) tGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAg < 2:2112662/100‑1 (MQ=255) tGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAg > 1:1647381/1‑100 (MQ=255) gCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAgg < 2:1672611/100‑1 (MQ=255) ccAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGc < 2:2138652/100‑1 (MQ=255) cAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGcc < 2:580260/100‑1 (MQ=255) tGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGtt < 1:2920407/100‑1 (MQ=255) ggggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTc > 1:1083663/1‑100 (MQ=255) gggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCa > 1:2987621/1‑100 (MQ=255) gggCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCa > 1:1608375/1‑100 (MQ=255) gCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAag > 1:1094073/1‑100 (MQ=255) gCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAag > 1:2598677/1‑100 (MQ=255) cGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagc < 1:1876749/100‑1 (MQ=255) cgcgcgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCTTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAagcagc > 2:1559133/1‑100 (MQ=255) cgGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGAt < 1:2357777/100‑1 (MQ=255) gCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCg < 2:2987605/100‑1 (MQ=255) cAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGt < 1:814569/100‑1 (MQ=255) cAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGt > 1:117295/1‑100 (MQ=255) aGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCa > 1:68150/1‑100 (MQ=255) cACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGc < 1:2752414/100‑1 (MQ=255) cACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGc > 2:289999/1‑100 (MQ=255) ccGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAg > 1:348026/1‑100 (MQ=255) cGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgc < 2:1647372/100‑1 (MQ=255) cGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgc > 2:2792326/1‑100 (MQ=255) ggCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAgcg < 2:1532923/100‑1 (MQ=255) aaGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCaa > 1:1492305/1‑100 (MQ=255) tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc < 2:1643525/100‑1 (MQ=255) tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc < 1:875882/100‑1 (MQ=255) tGCGCTTCATCAGCCATAATCCGGCGAATGATCCACCCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcc < 1:2842895/100‑1 (MQ=255) cgcTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcctg < 1:2636394/100‑1 (MQ=255) cTTCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTtgcctgcc < 2:2543422/100‑1 (MQ=255) ttCATCAGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTgcctgcct < 2:2781615/100‑1 (MQ=255) tcatcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCtt > 1:887119/1‑100 (MQ=255) catcaGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTg < 2:1766543/100‑1 (MQ=255) caGCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCg > 2:2962644/1‑100 (MQ=255) aGCCATAATCCGGCGAATGATCCACGCCG‑CCACGCCGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGt > 2:1466204/1‑100 (MQ=255) gCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTg > 2:1861477/1‑100 (MQ=255) gCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTg < 1:2730563/100‑1 (MQ=255) gCCATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTg > 1:762318/1‑100 (MQ=255) ccATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTgg < 1:2962660/100‑1 (MQ=255) cATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGc > 1:410378/1‑100 (MQ=255) cATAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGc > 1:2673055/1‑100 (MQ=255) aTAATCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCg < 2:170567/100‑1 (MQ=255) aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg > 1:587598/1‑100 (MQ=255) aaTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAg > 1:2217802/1‑100 (MQ=255) aaccGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc > 1:2009733/3‑100 (MQ=255) aTCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGc > 1:1449300/1‑100 (MQ=255) tCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCACTTTGCCTGCCTTGCCGTGGCGAGc > 1:1417998/1‑99 (MQ=255) tCCGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCa < 1:448768/100‑1 (MQ=255) ccGGCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCaa > 2:1985936/1‑100 (MQ=255) ggCGAATGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAc > 1:1582602/1‑100 (MQ=255) aTGATCCACGCCG‑CCCCGACGACATTAAAGGCGGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAAcgcg > 1:2830906/1‑98 (MQ=255) aTGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt < 1:2136944/100‑1 (MQ=255) aTGATCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGt < 2:117293/100‑1 (MQ=255) gATCCACGCCG‑CCACGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtat > 2:1841661/1‑100 (MQ=255) aTCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGtata > 1:1671003/1‑100 (MQ=255) aTCCACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCACCGGATCGTCAGGCTGTATGCGCAATTCGCCTGCCTTGCCGTGACGAGCAAACGCGGtata > 2:702709/1‑100 (MQ=255) ccACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATAc > 1:2315748/1‑100 (MQ=255) ccACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACg > 2:1641870/1‑100 (MQ=255) ccACGCCG‑CCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACg < 2:746788/100‑1 (MQ=255) cgccg‑cCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTg > 1:955394/1‑100 (MQ=255) | GGTGCCCGTTTTCCATGCCAGTGGGGCGACGCGCGGCAAGGCACTATCCGGCAAGGGTTGCGCTTCATCAGCCATAATCCGGCGAATGATCCACGCCGCCCCCGACGACATTAAAGGCCGTTCAAGCAGCGGATCGTCAGGCTGTAAGCGCAATTTGCCTGCCTTGCCGTGGCGAGCAAACGCGGTATACGCTG > NC_000913/2645784‑2645977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |