Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,304,813	(C)7→6	coding (1005/1245 nt)	mtr ←	tryptophan transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,304,807	0	C	.	100.0%	367.7 / NA	79	coding (1011/1245 nt)	mtr	tryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (40/39);  total (40/39)

CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAA  >  NC_000913/3304708‑3304901                                                                                                    |                                                                                               cgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGc                                                                                                >  1:1382945/1‑100 (MQ=255)  gATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcc                                                                                               <  1:2917979/100‑1 (MQ=255)  gATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGc                                                                                                <  1:600681/99‑1 (MQ=255)    tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                             <  2:906578/100‑1 (MQ=255)    tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                             <  2:377753/100‑1 (MQ=255)     gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                            >  2:2481514/1‑100 (MQ=255)     gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                            <  2:1413358/100‑1 (MQ=255)     gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                            <  2:1786022/100‑1 (MQ=255)     gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCCTACAGGAATCCGTTCGGTAACAACAGccctc                                                                                            <  1:2011521/100‑3 (MQ=255)      cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                           >  2:903208/1‑100 (MQ=255)      cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                           >  2:315246/1‑100 (MQ=255)       aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccca                                                                                          <  2:2407718/100‑2 (MQ=255)        cGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACGGccccccac                                                                                         <  1:1063283/100‑3 (MQ=255)          ngCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccacaa                                                                                       >  2:1605173/2‑96 (MQ=255)            cTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACt                                                                                    <  2:220591/100‑1 (MQ=255)             tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTg                                                                                   <  2:1097237/100‑1 (MQ=255)             tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTg                                                                                   <  1:1189502/100‑1 (MQ=255)              aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTgg                                                                                  >  1:2831874/1‑100 (MQ=255)               aCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGc                                                                                 <  2:1382937/100‑1 (MQ=255)                cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                                >  1:899729/1‑100 (MQ=255)                cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                                <  1:1379029/100‑1 (MQ=255)                  gcgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCggg                                                                              <  1:1106172/100‑1 (MQ=255)                   cgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCgggg                                                                             <  1:2607826/100‑1 (MQ=255)                       ggAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaaa                                                                         >  1:1638179/1‑100 (MQ=255)                       ggAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaaa                                                                         >  1:673675/1‑100 (MQ=255)                           cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     >  2:646787/1‑100 (MQ=255)                           cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     >  2:2145581/1‑100 (MQ=255)                           cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     >  1:2730362/1‑100 (MQ=255)                             aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAg                                                                   <  1:116264/100‑1 (MQ=255)                             aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAg                                                                   >  1:1745874/1‑100 (MQ=255)                               tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCa                                                                 >  2:2647799/1‑100 (MQ=255)                                gccgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCaa                                                                >  1:1219967/1‑100 (MQ=255)                                   gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGc                                                             <  1:2481529/100‑1 (MQ=255)                                    cccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGcc                                                            <  2:2680069/100‑1 (MQ=255)                                     ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                           <  1:716571/100‑1 (MQ=255)                                     ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                           <  2:288522/100‑1 (MQ=255)                                      cAGATGGTAGCCGCTAAACCAGCATAACCACTGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGt                                                          <  1:1605182/100‑1 (MQ=255)                                        gATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGttt                                                        >  1:1498613/1‑100 (MQ=255)                                        gATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGttt                                                        <  2:781241/100‑1 (MQ=255)                                         aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGtttt                                                       <  2:1925604/100‑1 (MQ=255)                                         aTGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGtttt                                                       >  1:1678623/1‑100 (MQ=255)                                           ggTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCa                                                     >  1:373869/1‑100 (MQ=255)                                             tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                   >  1:1461914/1‑100 (MQ=255)                                             tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                   >  1:2485498/1‑100 (MQ=255)                                              aGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGc                                                  <  1:945049/100‑1 (MQ=255)                                               gCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCg                                                 <  1:1632784/100‑1 (MQ=255)                                               gCCGCTAAACCAGCATAACCAATCGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGc                                                  <  1:2425962/99‑1 (MQ=255)                                                ccGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCgg                                                >  1:2332422/1‑100 (MQ=255)                                                 cGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                               >  1:1853386/1‑100 (MQ=255)                                                  gCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGcc                                              >  2:1901923/1‑100 (MQ=255)                                                       aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAg                                         >  1:2146242/1‑100 (MQ=255)                                                       aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTATCAAGCGGCCCACAg                                         >  1:2935298/1‑100 (MQ=255)                                                         cAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTTGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                       <  2:1290878/100‑1 (MQ=255)                                                           gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                     >  1:694905/1‑100 (MQ=255)                                                            cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                    <  2:277625/100‑1 (MQ=255)                                                            cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                    <  1:889313/100‑1 (MQ=255)                                                            cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                    >  2:23325/1‑100 (MQ=255)                                                            cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                    <  2:2299773/100‑1 (MQ=255)                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgt                                   <  2:1638170/100‑1 (MQ=255)                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgt                                   <  2:673672/100‑1 (MQ=255)                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgt                                   >  1:2434152/1‑100 (MQ=255)                                                             aTAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgt                                   >  1:1556160/1‑100 (MQ=255)                                                              tAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtc                                  >  1:699211/1‑100 (MQ=255)                                                               aaCCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcg                                 >  1:1413843/1‑100 (MQ=255)                                                                 ccAATGGCGTACAGGATTCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgtc                               <  2:1745863/100‑1 (MQ=255)                                                                 ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgtc                               <  1:199350/100‑1 (MQ=255)                                                                 ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgtc                               >  1:2192170/1‑100 (MQ=255)                                                                  cAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                              >  1:661084/1‑100 (MQ=255)                                                                      ggCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAc                          <  2:2192158/100‑1 (MQ=255)                                                                      ggCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAc                          <  2:1853375/100‑1 (MQ=255)                                                                       gCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAcc                         <  1:1901140/100‑1 (MQ=255)                                                                           aCAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                     <  1:1306376/100‑1 (MQ=255)                                                                            cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                    >  1:2217996/1‑100 (MQ=255)                                                                            cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                    >  2:2908826/1‑100 (MQ=255)                                                                            cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                    >  1:2324666/1‑100 (MQ=255)                                                                             aGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACan                   <  2:109730/100‑2 (MQ=255)                                                                             aGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                   <  2:901995/100‑1 (MQ=255)                                                                              ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGTAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                  >  2:1636993/1‑100 (MQ=255)                                                                              ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                  >  1:283057/1‑100 (MQ=255)                                                                              ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                  <  1:1903189/100‑1 (MQ=255)                                                                                aaTCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATc                >  2:46099/1‑100 (MQ=255)                                                                                  tCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTg              <  1:2804711/100‑1 (MQ=255)                                                                                  tCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAAGGCCGTTTTCAAGCGGCCCACAGCCGAATCGTCGAAACCAAACAGATCTg              <  2:1736894/100‑1 (MQ=255)                                                                                     gTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa           <  2:28205/100‑1 (MQ=255)                                                                                     gTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa           >  1:2252488/1‑100 (MQ=255)                                                                                        cGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGAt        >  2:2763047/1‑100 (MQ=255)                                                                                        cGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGAt        >  2:2707756/1‑100 (MQ=255)                                                                                         gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa       >  1:1487241/1‑100 (MQ=255)                                                                                           gAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt     >  2:2728120/1‑100 (MQ=255)                                                                                            aacaacAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTc    <  1:943394/100‑1 (MQ=255)                                                                                            aacaacAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTc    <  1:2425878/100‑1 (MQ=255)                                                                                             acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca   <  2:1109130/100‑1 (MQ=255)                                                                                              caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaa  >  1:876944/1‑100 (MQ=255)                                                                                                    |                                                                                               CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAA  >  NC_000913/3304708‑3304901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑