BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,809,774	(T)_7→8	coding (158/738 nt)	ygaZ →	putative L‑valine exporter, norvaline resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,809,767	1	.	T	93.2%	295.6 / 13.5	88	coding (151/738 nt)	ygaZ	putative L‑valine exporter, norvaline resistance protein
Reads supporting (aligned to +/- strand): ref base . (4/2); new base T (39/43); total (43/45)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809669‑2809860

gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t                                                                                                >  1:1276223/1‑100 (MQ=255)

gATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t                                                                                                >  2:339751/1‑100 (MQ=255)

 aTGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t                                                                                              >  2:2461621/1‑100 (MQ=255)

  tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑t                                                                                            <  1:371540/100‑1 (MQ=255)

  tGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑t                                                                                            >  2:1897014/1‑100 (MQ=255)

   gCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑tt                                                                                           <  1:1280963/100‑1 (MQ=255)

    cAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttt                                                                                          >  2:262780/1‑100 (MQ=255)

     aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑tttt                                                                                         <  1:426901/100‑1 (MQ=255)

     aaaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCGGAATGCGACTCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑tttt                                                                                         <  2:2444857/100‑1 (MQ=255)

      aaGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttt                                                                                        <  1:1696618/100‑1 (MQ=255)

        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttc                                                                                      <  1:106447/100‑3 (MQ=255)

        gACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttc                                                                                      >  2:1548468/1‑98 (MQ=255)

           aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttctcc                                                                                   <  1:1897025/100‑6 (MQ=255)

           aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttctcc                                                                                   <  1:385987/100‑6 (MQ=255)

           aGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttctcc                                                                                   <  1:446320/100‑6 (MQ=255)

            gTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑t‑t‑ttttttctcc                                                                                   >  2:1397499/1‑94 (MQ=255)

             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTg                                                                                  <  1:1079739/100‑1 (MQ=255)

             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTg                                                                                  <  1:753462/100‑1 (MQ=255)

             tttACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTg                                                                                  <  1:2536190/100‑1 (MQ=255)

               tACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGca                                                                                <  1:2727598/100‑1 (MQ=255)

                   gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGcatcat                                                                            >  2:1206214/1‑100 (MQ=255)

                   gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGcatcat                                                                            >  2:1789548/1‑100 (MQ=255)

                   gATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGcatcat                                                                            <  2:1555450/100‑1 (MQ=255)

                     ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCAttt                                                                          <  2:198565/100‑1 (MQ=255)

                     ttGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCAttt                                                                          >  2:2787463/1‑100 (MQ=255)

                     ttGTTATTAGTTATATTCCGGTAGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTTTCCTGCATCAttt                                                                          >  2:1733678/1‑100 (MQ=255)

                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTa                                                                         <  1:752421/100‑1 (MQ=255)

                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTa                                                                         <  1:1254428/100‑1 (MQ=255)

                      tGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTa                                                                         >  2:2427536/1‑100 (MQ=255)

                       gtacttaGTTATATTCCGGTGGCAATTGCGTTCGGTCTAAATGAGACATGTCTGGGATTCTCTCCTCTCGACAGCGG‑‑A‑T‑TTTTTCTCCTGcatcgtgtgt                                                                        >  2:200179/5‑94 (MQ=255)

                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTAt                                                                        <  1:1132764/100‑1 (MQ=255)

                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTAt                                                                        >  2:550921/1‑100 (MQ=255)

                       gTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTAt                                                                        >  1:1586559/1‑100 (MQ=255)

                        ttattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATg                                                                       <  1:1789559/100‑1 (MQ=255)

                         tattaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGc                                                                      >  2:2120466/1‑100 (MQ=255)

                          attaGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCa                                                                     <  1:2210435/100‑1 (MQ=255)

                            taGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCa                                                                     >  2:1145532/1‑98 (MQ=255)

                             aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTCTGAAGgc                                                                  >  1:2808118/1‑100 (MQ=255)

                             aGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAg                                                                    >  2:777713/1‑98 (MQ=255)

                              gTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGgcg                                                                 >  1:2677070/1‑100 (MQ=255)

                                tataTTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGcgcg                                                               >  1:2657229/1‑100 (MQ=255)

                                    ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  2:1686948/100‑1 (MQ=255)

                                    ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  1:973967/100‑1 (MQ=255)

                                    ttCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGcc                                                           <  2:2432127/100‑1 (MQ=255)

                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                         >  1:81295/1‑100 (MQ=255)

                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                         >  1:921466/1‑100 (MQ=255)

                                      ccGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAg                                                         >  2:181952/1‑100 (MQ=255)

                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                        >  1:2883426/1‑100 (MQ=255)

                                       cGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGt                                                        >  1:894447/1‑100 (MQ=255)

                                           ggCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                   >  2:1373681/1‑100 (MQ=255)

                                            gCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                   <  2:2045685/100‑1 (MQ=255)

                                             ccTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GTTT‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTc                                                   <  2:83535/100‑1 (MQ=255)

                                              cTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAt                                                 <  2:80546/100‑1 (MQ=255)

                                                  gCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑T‑TGTTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTAcc                                             >  2:651698/1‑100 (MQ=255)

                                                   cGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACcg                                            >  1:958546/1‑100 (MQ=255)

                                                        ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                       >  2:1269530/1‑100 (MQ=255)

                                                        ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                       >  1:2269407/1‑100 (MQ=255)

                                                        ggTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATg                                       >  2:749748/1‑100 (MQ=255)

                                                         gTCTGAATGCGACGCGTCTGGGATTCTCTCCTCTCGAAAGCGG‑‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGc                                      >  1:2016989/1‑100 (MQ=255)

                                                           cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTg                                    <  1:2381322/100‑1 (MQ=255)

                                                           cTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCAATACCGCGATGCTg                                    <  1:2028300/100‑1 (MQ=255)

                                                            tGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTgg                                   >  1:2631310/1‑100 (MQ=255)

                                                                  cGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTTTCTTTCTTCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGcc                              >  2:1142492/1‑99 (MQ=255)

                                                                  cGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCg                             >  1:2285876/1‑100 (MQ=255)

                                                                  agACTCGTCTTGGTTTGTCTCCTTTCGAACGCTT‑‑T‑T‑TTTTTCCCCTTTATCATTTATGCAGGCGCGAGCCAGTTCGCCGTTACCGTGGTGGTGGCAg                                <  2:1829637/96‑1 (MQ=255)

                                                                   gACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCgg                            >  1:781466/1‑100 (MQ=255)

                                                                    aCCCGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                           <  1:402518/100‑1 (MQ=255)

                                                                     cccGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCggg                           <  2:2677055/99‑1 (MQ=255)

                                                                     cccGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑TAT‑TTTTCCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGa                          <  2:2016977/100‑1 (MQ=255)

                                                                      ccGTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGag                         <  1:464175/100‑1 (MQ=255)

                                                                        gTCTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagta                       <  2:2285864/100‑1 (MQ=255)

                                                                          cTGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagtagt                     <  1:1145538/100‑1 (MQ=255)

                                                                           tGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTTTTTTTTTTCATTTATGCAGGCGCGAGCCACTTCGTCATTACCTCAATGCTGGCAGCCGGGAgttgtt                    >  2:2008568/1‑100 (MQ=255)

                                                                           tGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGagt                        <  1:1397507/96‑1 (MQ=255)

                                                                           tGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                    <  2:1578744/100‑1 (MQ=255)

                                                                           tGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                    <  1:963519/100‑1 (MQ=255)

                                                                           tGGGATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGtt                    <  1:1825806/100‑1 (MQ=255)

                                                                            gggATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                   >  2:1784686/1‑100 (MQ=255)

                                                                            gggATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTCCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGttt                   >  2:1648955/1‑100 (MQ=255)

                                                                              gATTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgt                 <  1:851398/100‑1 (MQ=255)

                                                                               aTTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  1:169830/100‑1 (MQ=255)

                                                                               aTTCTCTCCTCTCGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTtgtg                <  1:1076556/100‑1 (MQ=255)

                                                                                  ctctcctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt             <  2:2675612/100‑1 (MQ=255)

                                                                                  ctctcctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGAt             >  2:2713667/1‑100 (MQ=255)

                                                                                     tcctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgc          <  2:1586550/100‑1 (MQ=255)

                                                                                      cctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgct         >  2:2455860/1‑100 (MQ=255)

                                                                                       ctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  2:2453640/100‑1 (MQ=255)

                                                                                       ctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:1117737/100‑1 (MQ=255)

                                                                                       ctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        >  1:411530/1‑100 (MQ=255)

                                                                                       ctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:1548477/100‑1 (MQ=255)

                                                                                       ctctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctg        <  1:1648964/100‑1 (MQ=255)

                                                                                        tctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACAGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       >  2:2898405/1‑100 (MQ=255)

                                                                                         ctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCTCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       <  2:1299595/99‑1 (MQ=255)

                                                                                         ctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATtgctgc       <  1:320650/99‑1 (MQ=255)

                                                                                         ctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa      >  1:1997856/1‑100 (MQ=255)

                                                                                         ctcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCa      >  1:2149616/1‑100 (MQ=255)

                                                                                          tcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc     <  1:2443291/100‑1 (MQ=255)

                                                                                          tcGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCAc     >  1:1841582/1‑100 (MQ=255)

                                                                                           cGAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACt    <  2:435865/100‑1 (MQ=255)

                                                                                            gAAAGC‑GT‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTg   >  2:1134844/1‑100 (MQ=255)

GATGCAAAGACAGTTTACCGATTGTTATTAGTTATATTCCGGTGGCCTTTGCGTTCGGTCTGAATGCGACCCGTCTGGGATTCTCTCCTCTCGAAAGC‑G‑‑T‑T‑TTTTTCTCCTGCATCATTTATGCAGGCGCGAGCCAGTTCGTCATTACCGCGATGCTGGCAGCCGGGAGTAGTTTGTGGATTGCTGCACTGA  >  NC_000913/2809669‑2809860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`