Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,459,293 (G)5→6 coding (955/1482 nt) gspE → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,459,2881.G94.2% 305.2 / 11.3 88coding (950/1482 nt)gspEgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base G (28/54);  total (31/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459189‑3459382
                                                                                                      |                                                                                               
tGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCAtggt                                                                                                  <  2:2160047/100‑1 (MQ=255)
 ggAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCAtggtg                                                                                                >  1:1453335/1‑100 (MQ=255)
 ggAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCAtggtg                                                                                                >  1:387045/1‑100 (MQ=255)
   aaGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGTGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTggg                                                                                             <  2:628211/100‑1 (MQ=255)
   aaGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTggg                                                                                             >  2:2161982/1‑100 (MQ=255)
   aaGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTggg                                                                                             <  2:2832519/100‑1 (MQ=255)
   aaGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTggg                                                                                             <  1:2320860/100‑1 (MQ=255)
      gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggggg                                                                                          >  2:1521537/1‑99 (MQ=255)
      gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggggg                                                                                          <  2:2452784/100‑2 (MQ=255)
       cATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggggga                                                                                         <  2:950286/100‑3 (MQ=255)
       cATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTgggggga                                                                                         >  1:2585663/1‑98 (MQ=255)
        aTTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTggggggaa                                                                                        >  2:1216646/1‑97 (MQ=255)
           gggCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAAtt                                                                                      >  2:1887895/1‑100 (MQ=255)
           gggCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAAtt                                                                                      <  1:2612275/100‑1 (MQ=255)
           gggCAAACGCAGGTGAATACCCGTGTGGATAGGTCTTTCGCTCGCGGCGTGCGCGCCATGCTTCGCCAGGACCCGGATGGCGTCATGGTGGGGGGAAAtt                                                                                      <  2:2193147/100‑1 (MQ=255)
            ggCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTc                                                                                     >  1:532931/1‑100 (MQ=255)
             gCAAATGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCg                                                                                    <  2:1041840/100‑1 (MQ=255)
               aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  2:778283/100‑1 (MQ=255)
               aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTg                                                                                  <  1:1485800/100‑1 (MQ=255)
                aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGa                                                                                 >  1:1957299/1‑100 (MQ=255)
                aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCGGCGCGCCAAACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGa                                                                                 <  2:1281756/100‑1 (MQ=255)
                 aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGAt                                                                                <  1:1887906/100‑1 (MQ=255)
                 aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGAt                                                                                <  2:1413471/100‑1 (MQ=255)
                 aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGAt                                                                                <  1:2568620/100‑1 (MQ=255)
                  cGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATa                                                                               >  2:300493/1‑100 (MQ=255)
                   gCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATAc                                                                              <  2:702991/100‑1 (MQ=255)
                    cAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACa                                                                             >  1:618965/1‑100 (MQ=255)
                      ggTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGa                                                                           >  2:1534215/1‑100 (MQ=255)
                       gTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGaa                                                                          <  1:765105/100‑1 (MQ=255)
                        tGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGaaa                                                                         >  2:1501852/1‑100 (MQ=255)
                           aTACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCAGACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAg                                                                            <  2:1504870/94‑1 (MQ=255)
                             aCCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACcgcg                                                                    <  1:864787/100‑1 (MQ=255)
                              cccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCgcgc                                                                   >  1:734364/1‑100 (MQ=255)
                                 gtgtGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGa                                                                <  1:1976681/100‑1 (MQ=255)
                                  tgtgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAt                                                               <  2:2492689/100‑1 (MQ=255)
                                  tgtgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAt                                                               <  2:2677650/100‑1 (MQ=255)
                                  tgtgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAt                                                               <  1:208578/100‑1 (MQ=255)
                                  tgtgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAt                                                               <  2:1924727/100‑1 (MQ=255)
                                   gtgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAtt                                                              <  2:2971848/100‑1 (MQ=255)
                                    tgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGAtt                                                              >  1:548918/1‑99 (MQ=255)
                                    tgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTg                                                             <  2:2512172/100‑1 (MQ=255)
                                     ggATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGc                                                            <  2:1652190/100‑1 (MQ=255)
                                     ccgatctacTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCg                                                           <  2:310891/92‑1 (MQ=255)
                                       atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  1:477083/100‑1 (MQ=255)
                                       atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  1:2268587/100‑1 (MQ=255)
                                       atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:2646168/100‑1 (MQ=255)
                                       atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  1:2464571/100‑1 (MQ=255)
                                       atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGAACCCGGATGTCGTCATGGTAGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          >  2:1635334/1‑100 (MQ=255)
                                        tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGt                                                         <  1:2715222/100‑1 (MQ=255)
                                         atGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:352511/98‑1 (MQ=255)
                                          tGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCACAGATTGCGGTTc                                                       >  1:1737725/1‑100 (MQ=255)
                                            tCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAg                                                     >  1:1026780/1‑100 (MQ=255)
                                            acTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCgagatcgg                                                    >  1:310893/2‑93 (MQ=255)
                                             cTTTCGCTCGCGGTCTGCGCGCCATACTACGACAGGACCCGGATGTCGTCATGGAGGGGGGAAATTCGTGATACAGAAACCGCGCAGATCGCGGttga                                                      >  2:2586426/1‑96 (MQ=255)
                                              tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   >  2:1582118/1‑100 (MQ=255)
                                              tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   <  1:394539/100‑1 (MQ=255)
                                                 cgctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTc                                                >  2:683865/1‑100 (MQ=255)
                                                 cgctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTc                                                <  1:2328884/100‑1 (MQ=255)
                                                   ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  2:43992/1‑100 (MQ=255)
                                                   ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:2895/1‑100 (MQ=255)
                                                        ggCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGAAGATTGCGGTTCAGGCCTCGCTCAcc                                         >  2:598982/1‑100 (MQ=255)
                                                         gCCTGGGCGCCATACTTCGCCAGGACCCGGGTGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCGGCGCCCACCg                                        <  2:366877/100‑1 (MQ=255)
                                                                 gcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTg                                <  2:387043/100‑1 (MQ=255)
                                                                  ccATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTgg                               <  2:1453327/100‑1 (MQ=255)
                                                                  ccATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTgg                               <  2:1981198/100‑1 (MQ=255)
                                                                    ttccgatctcAGTACCCGGATGTCGTCATGGTGCGGTGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGt                              <  2:988320/90‑1 (MQ=255)
                                                                     tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            >  2:1642370/1‑100 (MQ=255)
                                                                     tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  2:589554/100‑1 (MQ=255)
                                                                     tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  2:2365915/100‑1 (MQ=255)
                                                                     tACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  1:2816489/100‑1 (MQ=255)
                                                                     gatctCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  1:1514914/96‑1 (MQ=255)
                                                                       cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  2:1692344/1‑100 (MQ=255)
                                                                       cTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          <  1:1773660/100‑1 (MQ=255)
                                                                        ttCGCCAGGACCCGGATGTCGTCATGGGGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActct                         <  1:1544384/100‑1 (MQ=255)
                                                                        ttCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:618962/100‑1 (MQ=255)
                                                                         tCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCGATCTGGTACtctc                        <  1:2005753/100‑1 (MQ=255)
                                                                         tCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACtctc                        <  2:2781948/100‑1 (MQ=255)
                                                                          cGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:2877198/98‑1 (MQ=255)
                                                                          cGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAcagatc                       >  2:1514905/1‑95 (MQ=255)
                                                                          cGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                       <  1:2832837/100‑1 (MQ=255)
                                                                          cGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                       <  1:2151973/100‑1 (MQ=255)
                                                                          cGCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                       <  2:403294/100‑1 (MQ=255)
                                                                           gCCAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCaa                      >  2:1101816/1‑100 (MQ=255)
                                                                             cAGGACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTagatcggaag                    >  1:988325/1‑91 (MQ=255)
                                                                                gACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTc                 >  1:903773/1‑100 (MQ=255)
                                                                                gACCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTc                 <  1:1904146/100‑1 (MQ=255)
                                                                                 aCCCGGATGTCGTCATGGTGGGGGGAAATTCTTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  1:1780226/100‑1 (MQ=255)
                                                                                 aCCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  1:933764/100‑1 (MQ=255)
                                                                                 aCCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  1:2874723/100‑1 (MQ=255)
                                                                                 aCCCGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  1:300495/100‑1 (MQ=255)
                                                                                  cccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCca               >  1:550277/1‑100 (MQ=255)
                                                                                  cccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCca               >  1:1647250/1‑100 (MQ=255)
                                                                                   ccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  1:574696/100‑1 (MQ=255)
                                                                                   ccGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  2:1759343/100‑1 (MQ=255)
                                                                                    cGGATGTCGTCATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcaca             >  1:810587/1‑100 (MQ=255)
                                                                                            gtcATGGTGGGGGGACATCCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGCTACTCTCAACACTCCACCCTAACAGt     >  2:1226063/1‑100 (MQ=255)
                                                                                             tcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTg    >  1:2136608/1‑100 (MQ=255)
                                                                                             tcATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTg    >  1:889473/1‑100 (MQ=255)
                                                                                              cATGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc   <  2:903770/100‑1 (MQ=255)
                                                                                               aTGGTGGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCa  <  2:442380/100‑1 (MQ=255)
                                                                                                      |                                                                                               
TGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459189‑3459382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: