BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,875	0	G	A	20.2%	277.2 / 10.0	126	intergenic (‑79/‑42)	rcnR/rcnA	transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
Reads supporting (aligned to +/- strand): ref base G (65/34); new base A (0/25); total (65/61)
Fisher's exact test for biased strand distribution p-value = 2.91e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

ATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGC  >  NC_000913/2185776‑2185973

aTCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggg                                                                                                    >  2:468523/1‑100 (MQ=255)

aTCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTggggggg                                                                                                    >  1:2643894/1‑100 (MQ=255)

 tCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCGGGTACTGGGGGGGa                                                                                                   >  2:2035036/1‑100 (MQ=255)

  cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGa                                                                                                   >  2:1379401/1‑99 (MQ=255)

  cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAg                                                                                                  >  1:1305782/1‑100 (MQ=255)

  cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACGATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAg                                                                                                  >  1:511122/1‑100 (MQ=255)

   aCGGATTGTATGAGACATGGAAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTGTCAGGTACTGGGGGGGAGt                                                                                                 <  1:622289/100‑1 (MQ=255)

     ggATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAg                                                                                               >  2:2027834/1‑100 (MQ=255)

     ggATTGTATGAGACATGGCAACACCTGGTTAAAAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGt                                                                                                 <  1:2666311/98‑1 (MQ=255)

       aTTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGaa                                                                                             >  2:749342/1‑100 (MQ=255)

          gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCa                                                                                          >  2:615089/1‑100 (MQ=255)

          gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCa                                                                                          >  1:2143332/1‑100 (MQ=255)

          gTATGAGACATGGAAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGGATCAGGTACTGGGGGGGAGTAGAATCa                                                                                          <  1:2956303/100‑1 (MQ=255)

           tATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAg                                                                                         >  1:2428997/1‑100 (MQ=255)

            aTGAGACGTGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGa                                                                                        >  1:1571365/1‑100 (MQ=255)

             tGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGAt                                                                                       >  2:1246215/1‑100 (MQ=255)

               agaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGGAGAATCaagat                                                                                      >  1:2045527/1‑95 (MQ=255)

               aaaCATTGAAACACCTGATTAACAAAAATATGAAAAATCATAGCACTATTAATCCACTGGGGGGTATTATCAGGTACTGCGGAGGAGTAGAATCAGAt                                                                                       <  2:1849897/96‑1 (MQ=255)

                    tgagaaCACCTGGTTAACAAGAATATAAAAAATTATAGCACTATTAAGCTACGGGGGGGTAGGATCAGGTACTGGGAGGGAGTAGAATCAGATTGCCGaa                                                                                <  2:1486960/96‑1 (MQ=255)

                    tGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGCGTAGAATCAGATTGCCGaa                                                                                >  1:2790062/1‑100 (MQ=255)

                     ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAAt                                                                               >  1:2468910/1‑100 (MQ=255)

                     ggCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGCGGGGAAGTATCAGGTACTGGGGGGGAGGAGAATCAGAGTACCGaa                                                                                >  2:1781510/1‑99 (MQ=255)

                      gCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTAGTAATCTAGTGGGGGGTAGTATCAGGTACTGGGGGAGAGTAGAATCAGATTGCCGAAtt                                                                              <  2:798110/100‑1 (MQ=255)

                         acaaCTGGTTAACAAGAATATGAAAAATTATAGCACTATTCGTCTACTAGGAGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGaa                                                                                <  2:795915/95‑1 (MQ=255)

                           acttGGATAACAAGAATATGAAAAATCATAGAACTATTAATTTACGGGTGAGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATAc                                                                         <  2:1545693/97‑1 (MQ=255)

                            ccTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                        >  2:1383936/1‑100 (MQ=255)

                            ccTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACt                                                                        >  1:428962/1‑100 (MQ=255)

                            ccTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAAGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATAc                                                                         <  2:2214765/99‑1 (MQ=255)

                              tGGTTAATAAGAATATGAAGAATCATATCTTTATTATTTTTGTGTGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTa                                                                       <  1:1034346/99‑1 (MQ=255)

                              tGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTaa                                                                      >  1:2074389/1‑100 (MQ=255)

                               ggTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAg                                                                     >  2:590857/1‑100 (MQ=255)

                                  tAACAAGAATATGAAAAATCAGAGCACTATGAATCTAATGGGTGGTAGTATCAATTACTGGTAAGAAGTAGAATCAGATTGCCGAATTAATACTAAGa                                                                    <  2:1955840/98‑1 (MQ=255)

                                   aagaaGAATATGAAAAATCATGGTACTATTAATATACGGGGGGGTAATATCAATTACTCGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAat                                                                  <  2:136051/96‑1 (MQ=255)

                                    aaaaGAATATGAAGAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                >  1:2020594/3‑100 (MQ=255)

                                    aCAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                >  1:2172569/1‑100 (MQ=255)

                                    aCAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                >  1:1254786/1‑100 (MQ=255)

                                    aCAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                >  1:82865/1‑100 (MQ=255)

                                     cAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattat                                                               >  2:2072351/1‑100 (MQ=255)

                                     cAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattat                                                               >  2:2621652/1‑100 (MQ=255)

                                      atgAATATGAAGAATTGTAGATCTTTTTATCCATTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                <  1:300725/96‑1 (MQ=255)

                                      aaGAATATGAAAAATCATATCACTATTAATCTACAAATGGGTAGAATCAATTACTAGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAatta                                                                <  2:428960/98‑1 (MQ=255)

                                      aaGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                                              >  1:2111937/1‑100 (MQ=255)

                                          atatTAAAAATCATAGCATTATTAATCGTATTTGTGGTTGGATCAATTAATGGTGAGAAGTATAATCAGATTGCCGAATTAATACTAAGAATtattat                                                            <  2:1097713/98‑1 (MQ=255)

                                          atatGAATAATCATTGTACTATTTATTTACTTGGGGGGAGGAGCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCa                                                          <  1:738962/100‑1 (MQ=255)

                                          agatGAAATAGCATAGCACTATGAAGCTACTGGGGGTTAGTATAAATTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCa                                                          <  2:2615534/98‑1 (MQ=255)

                                           tatGAAAAATCATAGCAGTATGAAGCTTCTGAGGGGTATTATCAAGTAATGGGAAGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCa                                                          <  2:2801317/99‑1 (MQ=255)

                                           tatGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCAt                                                         >  1:1715904/1‑100 (MQ=255)

                                            aggAAAAATCATAGCACTATTAATATACTGGGAGGTAGTATCAGGTACTAGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAATAATCATTATCa                                                          <  2:1571356/96‑1 (MQ=255)

                                              gAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                                      >  2:2925639/1‑100 (MQ=255)

                                              gAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                                      >  2:1420175/1‑100 (MQ=255)

                                              gAAAAATCAGAGCACTAGTAATCTACTGGGGGGTAGTATTAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAc                                                      <  2:180190/100‑1 (MQ=255)

                                               aaaaaTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGAcc                                                     >  1:178228/1‑100 (MQ=255)

                                                aaaaTCAGAGGATTATTAAACTTCTGGTGGGTAGTATGAGGTACTGGGAGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCa                                                          <  2:2820087/94‑1 (MQ=255)

                                                  aaTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGa                                                   >  2:2530237/1‑99 (MQ=255)

                                                  aaTCATAGAACTATTTATCTACTGGTGTTTAGTATCAGGTACTGGGTGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGaa                                                  <  1:2170915/100‑1 (MQ=255)

                                                       tAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTAc                                             >  2:1976442/1‑100 (MQ=255)

                                                       tAGAACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGATGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTAc                                             <  1:1105930/100‑1 (MQ=255)

                                                        aGCACTAGTAATCTACTGGGGGGTAGTCTAAAGTAATAGAAAGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTAc                                             <  2:2383108/99‑1 (MQ=255)

                                                         tcACTCTTATTCTACTGGTGGGTCGTGTTAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAAttt                                               <  1:1615608/95‑1 (MQ=255)

                                                         gcgctgtgaaTCTTCCGTGGGGTAGTCGCAGGGTCTGGTGAAAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACa                                            <  1:1884006/91‑1 (MQ=255)

                                                         gCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACaa                                           >  2:1803627/1‑100 (MQ=255)

                                                          cACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGAAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                          >  1:193072/1‑100 (MQ=255)

                                                          cACTATTAATCAACTGGGGGTTAGTATGAGCTACTGGTGGAGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                          <  1:835698/100‑1 (MQ=255)

                                                          cACTATGTACCTTCTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                          <  1:396949/100‑1 (MQ=255)

                                                           aCTATTAATCTACTGGGGGGTAGTATCAGTTACTTGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                          <  2:2951663/99‑1 (MQ=255)

                                                            cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                        >  2:1481001/1‑100 (MQ=255)

                                                            cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc                                        >  1:584426/1‑100 (MQ=255)

                                                             tATTAATCGACTGGGGGGGAGTATCAGGTACTGGGAGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc                                          <  2:1562697/97‑1 (MQ=255)

                                                              aTTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtctt                                      >  2:510668/1‑100 (MQ=255)

                                                              aTTAATCTACGGGTGAGTAGAATCAAGTAATTGTAAAAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACaa                                           <  2:2428982/95‑1 (MQ=255)

                                                                tAATGTACTAGGGAGTAGAAGCAATTAATGGTGAGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTa                                              <  2:2786576/90‑1 (MQ=255)

                                                                 aaTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt                                   >  2:1694183/1‑100 (MQ=255)

                                                                  aTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCttcttc                                  >  2:1561347/1‑100 (MQ=255)

                                                                  aTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCttcttc                                  >  1:2673811/1‑100 (MQ=255)

                                                                   tCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCa                                 >  2:470645/1‑100 (MQ=255)

                                                                     ttatGGGGGGTAGTATCAAGTACTGGTAAGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCa                                 <  2:1366704/95‑1 (MQ=255)

                                                                     tACTGAGTGGTAGTATCAATTACTGGTGAGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGc                               <  2:2074377/100‑1 (MQ=255)

                                                                        tGGGGGGTAGTATGAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAg                            >  1:2028618/1‑100 (MQ=255)

                                                                         ggggggTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAgg                           >  1:1412825/1‑100 (MQ=255)

                                                                         ggggggTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAgg                           >  1:1003615/1‑100 (MQ=255)

                                                                               tagtaTCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGc                     >  2:907401/1‑100 (MQ=255)

                                                                                agtaTCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGcc                    >  2:2176217/1‑100 (MQ=255)

                                                                                  taTCAGGTATTAGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg                  <  2:1275/100‑1 (MQ=255)

                                                                                  taTCAGGGACTGGGGGGGAGTAGAATCATATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTg                  <  1:615092/100‑1 (MQ=255)

                                                                                   aTCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTgg                 >  2:830381/1‑100 (MQ=255)

                                                                                   aTCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTgg                 >  2:1088606/1‑100 (MQ=255)

                                                                                    tgagtgacgggggtgaAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt                <  2:1283221/91‑1 (MQ=255)

                                                                                     cAGTTACTAGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCttcttc                                  <  2:2646476/81‑1 (MQ=255)

                                                                                     cAGGTACGGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGtt               >  1:1568213/1‑100 (MQ=255)

                                                                                       tggaCTGGTGGAGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttct             <  1:1661362/97‑1 (MQ=255)

                                                                                        gTACGGGTGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttctt            <  1:590860/100‑1 (MQ=255)

                                                                                         taatGGGAGGTAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt                <  2:1003704/92‑1 (MQ=255)

                                                                                          aCTGTGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAGACGCCTGGttct             <  2:1722022/97‑1 (MQ=255)

                                                                                           cTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCAt         >  2:147259/1‑100 (MQ=255)

                                                                                           cTGGGGGGAAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACCCCTGGTTCTTCAt         <  1:2027846/100‑1 (MQ=255)

                                                                                            ngggggggAGTAGAATCAGAGTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATc        >  2:1822051/2‑100 (MQ=255)

                                                                                             gggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATcc       >  1:905943/1‑100 (MQ=255)

                                                                                             gggggggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATcc       >  2:2761036/1‑100 (MQ=255)

                                                                                                 gggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttctt            <  2:2367256/91‑1 (MQ=255)

                                                                                                  ggAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAgc  >  2:2598824/1‑100 (MQ=255)

ATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTTCATCCCCAGC  >  NC_000913/2185776‑2185973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`