Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,143,641 G→A H243Y (CAT→TAT)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,143,6410GA99.2% 447.9 / ‑4.4 126H243Y (CAT→TAT) rneendoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (63/62);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 126 total aligned reads displayed.

ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736
                                                                                                 |                                                                                               
    gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGa                                                                                             >  2:1344178/1‑98 (MQ=255)
    gtgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACg                                                                                           >  1:603531/1‑100 (MQ=255)
     tgACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGt                                                                                          <  2:964637/100‑1 (MQ=255)
      gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         <  2:2852558/100‑1 (MQ=255)
      gACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTg                                                                                         <  1:512888/100‑1 (MQ=255)
        cTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGcc                                                                                       <  2:2170220/100‑1 (MQ=255)
         tCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCa                                                                                      <  1:2564143/100‑1 (MQ=255)
          cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     <  2:2867642/100‑1 (MQ=255)
          cGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAg                                                                                     >  1:1534842/1‑100 (MQ=255)
             tCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTc                                                                                  <  2:1184106/100‑1 (MQ=255)
              cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  2:1543875/100‑1 (MQ=255)
              cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 >  1:626243/1‑100 (MQ=255)
              cTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCg                                                                                 <  1:1618677/100‑1 (MQ=255)
                ggaaGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAAGGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               >  2:1141118/4‑100 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  2:2552723/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:2452124/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:2917529/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:1350961/100‑1 (MQ=255)
                ggTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAg                                                                               <  1:1238803/100‑1 (MQ=255)
                 gTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTCTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGc                                                                              <  2:2303360/100‑1 (MQ=255)
                   aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCAc                                                                            <  2:2705086/100‑1 (MQ=255)
                   aGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCAc                                                                            >  2:1039820/1‑100 (MQ=255)
                    gTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACt                                                                           >  2:446696/1‑100 (MQ=255)
                     tGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACtt                                                                          <  2:101269/100‑1 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:2669762/1‑100 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:2570525/1‑100 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  1:303796/1‑100 (MQ=255)
                      ggCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTGTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACttt                                                                         >  2:1836810/1‑100 (MQ=255)
                          gAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCggg                                                                     <  1:2801030/100‑1 (MQ=255)
                            aCAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGtt                                                                   >  1:733365/1‑100 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  >  1:327623/1‑100 (MQ=255)
                             cAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTa                                                                  <  1:2261622/100‑1 (MQ=255)
                               gCGGGATCTCGCTGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:787316/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:539534/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:2125071/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  1:1195799/100‑1 (MQ=255)
                               gCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                >  2:2208565/1‑100 (MQ=255)
                                cGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCg                                                               <  2:1870915/100‑1 (MQ=255)
                                 gggATCTGGCCGGTGTACAGTTTGATTTTGCGGCTGGAATCCGGGCGACCTCATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATc                                                                <  2:2513206/98‑1 (MQ=255)
                                 gggATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGACATTTCGAGCACTTTCGGGTTATc                                                                >  2:1026771/1‑98 (MQ=255)
                                   gATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATa                                                            <  2:2809480/100‑1 (MQ=255)
                                     tctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAg                                                          <  2:2340522/100‑1 (MQ=255)
                                     tctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAg                                                          <  2:2906142/100‑1 (MQ=255)
                                      ctcGCCGGTTTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACATGACAGTTCGAGCACTTTCGGGTTATCGATAAg                                                          <  2:1966035/99‑1 (MQ=255)
                                      ctcGCCGGTGTCCAGTTTGATTTTGCTGCTGAACTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATaatg                                                         <  2:2041736/100‑3 (MQ=255)
                                      ctcGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAgg                                                         >  1:1514198/1‑100 (MQ=255)
                                        cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       >  2:819919/1‑100 (MQ=255)
                                        cGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAt                                                       >  1:2062158/1‑100 (MQ=255)
                                          ccGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGAttt                                                     <  2:1922997/100‑1 (MQ=255)
                                             gtgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGc                                                  <  2:626240/100‑1 (MQ=255)
                                             gtgtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGc                                                  <  2:69629/100‑1 (MQ=255)
                                               gtACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCg                                                <  1:227176/100‑1 (MQ=255)
                                                tACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGa                                               >  1:1165615/1‑100 (MQ=255)
                                                 aCAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGAt                                              >  2:943965/1‑100 (MQ=255)
                                                  cAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATg                                             >  2:66464/1‑100 (MQ=255)
                                                    gTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTc                                           >  1:1104953/1‑100 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  1:2185974/100‑1 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         >  2:879890/1‑100 (MQ=255)
                                                      ttGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         <  2:13898/100‑1 (MQ=255)
                                                       tGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCt                                         >  2:1459854/1‑99 (MQ=255)
                                                        gATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGa                                       >  2:1694706/1‑100 (MQ=255)
                                                         aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      >  1:1689138/1‑100 (MQ=255)
                                                         aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      >  1:1978426/1‑100 (MQ=255)
                                                         aTTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGAc                                      >  2:1115254/1‑100 (MQ=255)
                                                            ttGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgta                                   <  1:2209980/100‑1 (MQ=255)
                                                             tgctgcTGAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGGTATCGATAAGGATTTCGCCGATGTCCTGACgt                                    >  2:127522/1‑98 (MQ=255)
                                                               ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                >  1:722086/1‑100 (MQ=255)
                                                               ctgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagt                                <  2:2287809/100‑1 (MQ=255)
                                                                tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  2:2755974/1‑100 (MQ=255)
                                                                tgctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagta                               >  1:102244/1‑100 (MQ=255)
                                                                 gctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACgtaagtaa                              >  2:2821076/1‑100 (MQ=255)
                                                                  ctgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAAt                             >  1:2490195/1‑100 (MQ=255)
                                                                   tgAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATc                            >  2:1882266/1‑100 (MQ=255)
                                                                    gAAATCCGGGCGCCCTAATGCAGCGATATATTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGCTTTCGCCGATGTCCTGACATAAGTAATc                            >  2:1959132/1‑99 (MQ=255)
                                                                    gAAATCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcg                           >  1:1252852/1‑100 (MQ=255)
                                                                      aaTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATcgcg                         >  2:1281560/1‑100 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        >  2:481376/1‑100 (MQ=255)
                                                                       aTCCGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCgg                        <  2:1689127/100‑1 (MQ=255)
                                                                         ccGGGCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATACGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGaa                      >  2:2537389/1‑100 (MQ=255)
                                                                             gCGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcg                  <  2:2118021/100‑1 (MQ=255)
                                                                              cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgcgc                 <  1:209665/100‑1 (MQ=255)
                                                                              cGACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATgccc                 <  1:2024976/100‑3 (MQ=255)
                                                                               gACCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGcgcg                >  1:2559206/1‑100 (MQ=255)
                                                                                aCCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa               >  2:2074729/1‑100 (MQ=255)
                                                                                aCCTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGa               >  2:1834929/1‑100 (MQ=255)
                                                                                  cTAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAAc             >  1:726172/1‑100 (MQ=255)
                                                                                   tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg            <  1:819922/100‑1 (MQ=255)
                                                                                   tAATGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACg            >  2:2063563/1‑100 (MQ=255)
                                                                                     aTGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAt          <  1:1836821/100‑1 (MQ=255)
                                                                                      tGCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGAtt         >  1:1295208/1‑100 (MQ=255)
                                                                                       gCAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTa        >  2:2902947/1‑100 (MQ=255)
                                                                                        cAGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTAc       <  2:2062146/100‑1 (MQ=255)
                                                                                         aGCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACg      <  2:1534833/100‑1 (MQ=255)
                                                                                          gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     >  2:61393/1‑100 (MQ=255)
                                                                                          gCGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGt     <  1:1535987/100‑1 (MQ=255)
                                                                                           cGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGtt    >  2:2227501/1‑100 (MQ=255)
                                                                                           cGATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGtt    <  1:377795/100‑1 (MQ=255)
                                                                                            gATATACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGtt    >  2:1939079/1‑99 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  1:483916/100‑1 (MQ=255)
                                                                                             atatACTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGc  <  2:978542/100‑1 (MQ=255)
                                                                                                 |                                                                                               
ATCTGTGACTCGATCTGGTAGTGGCTGAACAGCGGGATCTCGCCGGTGTACAGTTTGATTTTGCTGCTGAAATCCGGGCGACCTAATGCAGCGATATGCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGC  >  NC_000913/1143544‑1143736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: