Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,516,689 T→C T382A (ACG→GCG)  yfeA ← putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,516,6890TC99.0% 376.9 / ‑4.5 104T382A (ACG→GCG) yfeAputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (48/55);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 104 total aligned reads displayed.

CGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGC  >  NC_000913/2516595‑2516787
                                                                                              |                                                                                                  
cGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGc                                                                                               <  1:479442/100‑1 (MQ=255)
  cccTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACACCTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCaa                                                                                             >  2:1388659/1‑100 (MQ=255)
    cTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAt                                                                                           <  1:955803/100‑1 (MQ=255)
    cTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAt                                                                                           >  1:1745581/1‑100 (MQ=255)
     ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAtt                                                                                          >  2:1368000/1‑100 (MQ=255)
     ttCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAAtt                                                                                          >  2:1720590/1‑100 (MQ=255)
      tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCACTTGATACAACTTTTCGTTTTCCTGCATCACCGGCAGCAGCGCACGGCAAAtt                                                                                          >  2:1365318/1‑99 (MQ=255)
      tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                         <  1:1640487/100‑1 (MQ=255)
      tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                         <  1:868570/100‑1 (MQ=255)
      tCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTg                                                                                         >  2:1574879/1‑100 (MQ=255)
        cGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAg                                                                                       <  2:2163393/100‑1 (MQ=255)
        cGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAg                                                                                       >  1:2249958/1‑100 (MQ=255)
         gTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGc                                                                                      <  2:295576/100‑1 (MQ=255)
         gTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGc                                                                                      >  1:1428618/1‑100 (MQ=255)
          tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCGGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                     <  2:1480573/100‑1 (MQ=255)
          tttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCg                                                                                     <  1:1913709/100‑1 (MQ=255)
           ttCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCgg                                                                                    <  2:2157936/100‑1 (MQ=255)
             ccGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGAt                                                                                  >  2:2653712/1‑100 (MQ=255)
              cGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATa                                                                                 <  1:877097/100‑1 (MQ=255)
                 cccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAg                                                                              >  2:2761295/1‑100 (MQ=255)
                 cccGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAg                                                                              <  2:1037864/100‑1 (MQ=255)
                   cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                            <  1:2560662/100‑1 (MQ=255)
                   cGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTg                                                                            >  2:137813/1‑100 (MQ=255)
                     cTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGaa                                                                          >  1:699483/1‑100 (MQ=255)
                      tCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAc                                                                         >  1:561032/1‑100 (MQ=255)
                      tCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAc                                                                         <  1:126522/100‑1 (MQ=255)
                        aGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgc                                                                       >  2:1964841/1‑100 (MQ=255)
                         gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATCCAGTGAAcgcg                                                                      >  1:2490756/1‑100 (MQ=255)
                         gCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAAcgcg                                                                      <  1:1629324/100‑1 (MQ=255)
                          cACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACgcgc                                                                     >  2:25744/1‑100 (MQ=255)
                          cACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACgcgc                                                                     >  2:1361112/1‑100 (MQ=255)
                           aatAACAGCAGTTAACTACCCGGCAATTGATTCAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGca                                                                    >  1:2007898/3‑100 (MQ=255)
                           aCTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGca                                                                    <  1:1680249/100‑1 (MQ=255)
                           aCTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGca                                                                    <  1:2777821/100‑1 (MQ=255)
                            cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                   <  2:2052199/100‑1 (MQ=255)
                            cTAACAGCAGTTCACTACCCGGCAATTGATACAACTTCTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcat                                                                   <  2:2007886/100‑1 (MQ=255)
                             tAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatc                                                                  >  2:1582607/1‑100 (MQ=255)
                             tAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatc                                                                  <  1:2970121/100‑1 (MQ=255)
                              aaCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                                 >  2:2263112/1‑100 (MQ=255)
                              aaCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatca                                                                 <  2:615575/100‑1 (MQ=255)
                              aaCAGCAGTTCACTACCCGGCAATTGATACAACTTATCGTATTCCTGCATCAACGGCAGCAGCGCACGGCACATTGAGCGGATACAATGGACGCGcatca                                                                 >  2:814881/1‑100 (MQ=255)
                               aCAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGcatcat                                                                >  1:2417187/1‑100 (MQ=255)
                                cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                               >  2:736059/1‑100 (MQ=255)
                                cagcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCAtt                                                               >  2:2458532/1‑100 (MQ=255)
                                  gcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTaa                                                             >  2:1507038/1‑100 (MQ=255)
                                  gcagTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTaa                                                             <  1:1714057/100‑1 (MQ=255)
                                    agTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                           <  1:2578446/100‑1 (MQ=255)
                                    agTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGc                                                           <  2:1513845/100‑1 (MQ=255)
                                       tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                        <  1:1574888/100‑1 (MQ=255)
                                       tCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGt                                                        >  2:1904683/1‑100 (MQ=255)
                                        cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTa                                                       <  2:380402/100‑1 (MQ=255)
                                        cACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTa                                                       <  1:1665146/100‑1 (MQ=255)
                                          cTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAAt                                                     <  2:544491/100‑1 (MQ=255)
                                            aCCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAACGCACGGCAAATTGAGCGGATACCGTGAACGCGCATCATTAAGCCGTaa                                                      >  2:1079446/1‑97 (MQ=255)
                                              ccGGCAATTGATACAACTTTTCGTTTTCCTGCGTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                                 <  1:571169/100‑1 (MQ=255)
                                              ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                                 >  2:2977960/1‑100 (MQ=255)
                                              ccGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacg                                                 <  2:227589/100‑1 (MQ=255)
                                               cGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacga                                                >  1:1264916/1‑100 (MQ=255)
                                               cGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacga                                                <  2:1880554/100‑1 (MQ=255)
                                                ggCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATgacgac                                               <  2:413414/100‑1 (MQ=255)
                                                  cAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTc                                             >  1:1792468/1‑100 (MQ=255)
                                                   aaTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCa                                            <  1:1295967/100‑1 (MQ=255)
                                                    aTTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCAt                                           <  1:2062181/100‑1 (MQ=255)
                                                       gATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATaaa                                        <  2:83729/100‑1 (MQ=255)
                                                        aTACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAAc                                       <  1:1720601/100‑1 (MQ=255)
                                                         tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                      <  2:2456543/100‑1 (MQ=255)
                                                         tACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACt                                      <  2:615429/100‑1 (MQ=255)
                                                          aCAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTc                                     >  1:97240/1‑100 (MQ=255)
                                                           cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                    >  1:238875/1‑100 (MQ=255)
                                                           cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                    >  1:1405067/1‑100 (MQ=255)
                                                           cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                    <  1:675896/100‑1 (MQ=255)
                                                           cAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCa                                    >  2:945887/1‑100 (MQ=255)
                                                               ttttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATAAAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAt                                >  2:1642432/1‑100 (MQ=255)
                                                                tttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAtt                               >  1:268649/1‑100 (MQ=255)
                                                                tttCGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGAtt                               >  2:1640498/1‑100 (MQ=255)
                                                                   cGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATc                            >  2:907796/1‑100 (MQ=255)
                                                                   cGTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATc                            >  1:2395725/1‑100 (MQ=255)
                                                                    gTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCa                           <  2:396504/100‑1 (MQ=255)
                                                                    gTTTTCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCa                           <  2:2417172/100‑1 (MQ=255)
                                                                     ttttCCTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTAtaga                          >  1:986595/1‑97 (MQ=255)
                                                                         ccTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcg                      >  2:959367/1‑100 (MQ=255)
                                                                         ccTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcg                      >  2:1116742/1‑100 (MQ=255)
                                                                          cTGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATgcgc                     >  1:2005937/1‑100 (MQ=255)
                                                                           tGCATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgca                    <  1:2953347/100‑1 (MQ=255)
                                                                             cATCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcagg                  >  2:2463072/1‑100 (MQ=255)
                                                                              aTCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCAGCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggc                 >  2:245855/1‑100 (MQ=255)
                                                                               tCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggca                <  2:1460291/100‑1 (MQ=255)
                                                                               tCAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggca                <  1:719451/100‑1 (MQ=255)
                                                                                cAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggcag               >  2:2169637/1‑100 (MQ=255)
                                                                                cAACGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCgcaggcag               >  2:1698805/1‑100 (MQ=255)
                                                                                 aaCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGgcagc              <  1:2147518/100‑1 (MQ=255)
                                                                                  aCGGCAGCAGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGgcagca             <  1:1704490/100‑1 (MQ=255)
                                                                                     gcagcaGCGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCaaaa          <  1:2891230/100‑1 (MQ=255)
                                                                                       agcagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAc        <  1:1582616/100‑1 (MQ=255)
                                                                                       agcagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAc        <  1:505036/100‑1 (MQ=255)
                                                                                       agcagcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAAc        <  1:2653727/100‑1 (MQ=255)
                                                                                          agcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActct     <  1:2761311/100‑1 (MQ=255)
                                                                                          agcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActct     <  1:1116748/100‑1 (MQ=255)
                                                                                          agcGCACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAActct     <  2:2249946/100‑1 (MQ=255)
                                                                                             gTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGc  <  1:359468/100‑1 (MQ=255)
                                                                                              |                                                                                                  
CGCCCTTCCGTTTCCGGCCCGCTCAGCACTAACAGCAGTTCACTACCCGGCAATTGATACAACTTTTCGTTTTCCTGCATCAACGGCAGCAGCGTACGGCAAATTGAGCGGATACAGTGAACGCGCATCATTAAGCCGTAATGACGACTCATAAACTCAAGATTATCAATGCGCAGGCAGCAAAAACTCTTGC  >  NC_000913/2516595‑2516787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑