Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,397,557	G→T	G49V (GGT→GTT)	mutL →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,397,557	0	G	T	100.0%	366.6 / NA	100	G49V (GGT→GTT)	mutL	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (52/48);  total (52/48)

GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652                                                                                                   |                                                                                                ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGC‑ACGCGTTg                                                                                               >  1:243979/1‑100 (MQ=255) ggtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTg                                                                                               >  2:1619509/1‑100 (MQ=255)  gtgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgg                                                                                              <  2:2443459/100‑1 (MQ=255)   tgaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTggg                                                                                             <  1:2805470/100‑1 (MQ=255)    gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGATACGCGTTggg                                                                                             <  2:941741/100‑1 (MQ=255)    gaggtgGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            >  2:893230/1‑100 (MQ=255)      ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  1:1370215/1‑100 (MQ=255)      ggtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCg                                                                                          >  1:1370388/1‑100 (MQ=255)       gtggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTgggg                                                                                            <  1:22255/97‑1 (MQ=255)         ggtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaaa                                                                                       >  1:2740255/1‑100 (MQ=255)          gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAAc                                                                                      <  2:1239211/100‑1 (MQ=255)          gtCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCATCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGaa                                                                                        <  2:858055/98‑1 (MQ=255)           tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     <  2:2659397/100‑1 (MQ=255)           tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     >  2:2781170/1‑100 (MQ=255)           tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     <  1:2501004/100‑1 (MQ=255)           tCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACt                                                                                     <  1:715517/100‑1 (MQ=255)             gAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTa                                                                                   <  1:2077430/100‑1 (MQ=255)              aGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTAt                                                                                  >  2:1183051/1‑100 (MQ=255)               gCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATc                                                                                 <  1:2129979/100‑1 (MQ=255)               gCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATc                                                                                 >  1:2204317/1‑100 (MQ=255)                   ccTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCa                                                                             >  2:2489531/1‑100 (MQ=255)                    cTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCAt                                                                            >  2:852690/1‑100 (MQ=255)                       cGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCg                                                                         >  1:1735594/1‑100 (MQ=255)                          cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      >  1:2798338/1‑100 (MQ=255)                          cGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGa                                                                      <  1:660791/100‑1 (MQ=255)                           ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     <  1:1867900/100‑1 (MQ=255)                           ggTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGAt                                                                     >  1:1525540/1‑100 (MQ=255)                            gTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATa                                                                    <  2:2798322/100‑1 (MQ=255)                             tAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATaa                                                                   <  1:2633140/100‑1 (MQ=255)                              aGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAAc                                                                  <  2:2478463/100‑1 (MQ=255)                               gTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACg                                                                 >  2:861608/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:2062546/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1042052/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1320515/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:1806981/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:2564117/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:2481370/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  1:1842285/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:2886484/1‑100 (MQ=255)                                 cAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGc                                                               >  2:398135/1‑100 (MQ=255)                                    aGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGc                                                            <  1:2781186/100‑1 (MQ=255)                                     gAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCg                                                           <  2:1891233/100‑1 (MQ=255)                                      aaCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCgg                                                          <  2:2781614/100‑1 (MQ=255)                                       aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                         >  1:1434501/1‑100 (MQ=255)                                       aCTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGt                                                         <  2:2257307/100‑1 (MQ=255)                                        cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        >  2:2602390/1‑100 (MQ=255)                                        cTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTa                                                        <  2:1943703/100‑1 (MQ=255)                                         tAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTAt                                                       <  2:2175254/100‑1 (MQ=255)                                          aGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      <  1:1613709/100‑1 (MQ=255)                                           gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATc                                                      >  2:1946860/1‑99 (MQ=255)                                           gTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCa                                                     >  2:735756/1‑100 (MQ=255)                                            tGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                    >  2:1034372/1‑100 (MQ=255)                                            tGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaa                                                    <  2:2481355/100‑1 (MQ=255)                                             ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  2:2432375/1‑100 (MQ=255)                                             ggAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaa                                                   >  2:174287/1‑100 (MQ=255)                                               aaaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaa                                                 <  1:1042057/100‑1 (MQ=255)                                                aaaCAGCCTGGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  2:828768/100‑1 (MQ=255)                                                aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  2:974504/100‑1 (MQ=255)                                                aaaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCaaaaaa                                                <  1:2509203/100‑1 (MQ=255)                                                 aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAg                                               <  2:1326186/100‑1 (MQ=255)                                                 aaCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGATGAAGAATAAAAAAAg                                               <  2:2536748/100‑1 (MQ=255)                                                  aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTTATAACGGCTAAAGTATCAAAAAAGa                                              <  2:2978205/100‑1 (MQ=255)                                                  aCAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGa                                              <  2:875860/100‑1 (MQ=255)                                                   cAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGAt                                             >  1:2975605/1‑100 (MQ=255)                                                    aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            >  1:1703761/1‑100 (MQ=255)                                                    aGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATg                                            <  2:1067410/100‑1 (MQ=255)                                                     gCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGa                                           <  2:2242915/100‑1 (MQ=255)                                                     gCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGa                                           >  2:1071126/1‑100 (MQ=255)                                                      ccTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAg                                          >  2:837554/1‑100 (MQ=255)                                                           aTGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggc                                     <  1:1040604/100‑1 (MQ=255)                                                              cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  <  2:2316418/100‑1 (MQ=255)                                                              cAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgct                                  >  1:1936937/1‑100 (MQ=255)                                                                  tGCGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcg                              >  2:420330/1‑100 (MQ=255)                                                                    cGACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgct                            <  1:2780264/100‑1 (MQ=255)                                                                     gACGCGTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctg                           <  1:151288/100‑1 (MQ=255)                                                                       cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         >  2:1493125/1‑100 (MQ=255)                                                                       cgcgTATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAgctggcgctggcgctggc                         <  1:2602405/100‑1 (MQ=255)                                                                          cgttCGATATCAATATCGATACGCGTCGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTc                       >  1:1554969/4‑100 (MQ=37)                                                                           tATCGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGt                     >  2:700306/1‑100 (MQ=255)                                                                              cGATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCAt                  <  1:370320/100‑1 (MQ=255)                                                                               gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 >  2:2665773/1‑100 (MQ=255)                                                                               gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 >  2:1400842/1‑100 (MQ=255)                                                                               gATATTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTAAAAAAAAAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATg                 <  2:2419843/100‑1 (MQ=255)                                                                                 tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               <  1:823457/100‑1 (MQ=255)                                                                                 tatTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcc               >  1:13296/1‑100 (MQ=255)                                                                                  atTGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGcca              >  2:2478604/1‑100 (MQ=255)                                                                                   ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  2:1444143/1‑100 (MQ=255)                                                                                   ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  1:2080211/1‑100 (MQ=255)                                                                                   ttGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccac             >  1:947635/1‑100 (MQ=255)                                                                                    tGATATCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  1:1664058/100‑1 (MQ=255)                                                                                    tGATATCGA‑AAGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccacc            <  1:420332/100‑1 (MQ=255)                                                                                      atatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAg          <  1:837557/100‑1 (MQ=255)                                                                                       tatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGccaccggt         >  1:1525097/1‑97 (MQ=255)                                                                                       tatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGt         >  1:304284/1‑100 (MQ=255)                                                                                       tatCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGt         <  2:1225771/100‑1 (MQ=255)                                                                                        atCGA‑ACGCGTTGGGGCGAAACTTATCCGCATTTGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTa        >  2:2552207/1‑100 (MQ=255)                                                                                          cGA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaa      >  1:1325600/1‑100 (MQ=255)                                                                                           gA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     >  1:1490662/1‑100 (MQ=255)                                                                                           gA‑ACGCGTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTaaaa     <  1:1400850/100‑1 (MQ=255)                                                                                               cgcgTTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCg  <  1:2674737/100‑1 (MQ=255)                                                                                                   |                                                                                                GGTGAGGTGGTCGAGCGACCTGCGTCGGTAGTCAAAGAACTAGTGGAAAACAGCCTCGATGCAGGTGCGACGCGTATCGATATTGATATCGA‑ACGCGGTGGGGCGAAACTTATCCGCATTCGTGATAACGGCTGCGGTATCAAAAAAGATGAGCTGGCGCTGGCGCTGGCTCGTCATGCCACCAGTAAAATCG  >  NC_000913/4397460‑4397652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑